Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
857,539 |
T→C |
E175G (GAA→GGA) |
ybiW ← |
glycyl radical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 857,539 | 0 | T | C | 100.0%
| 102.6
/ NA
| 28 | E175G (GAA→GGA) | ybiW | glycyl radical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/7); total (21/7) |
CTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGAC > NZ_CP009273/857454‑857626
|
cTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCcagc < 1:517976/91‑1 (MQ=255)
ccGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCcagcagca > 1:92573/1‑91 (MQ=255)
gtgtAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCagcagcagc > 1:941615/1‑91 (MQ=255)
gtAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCgg > 1:50871/1‑91 (MQ=255)
tCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCa > 1:197123/1‑91 (MQ=255)
tCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCa > 1:466909/1‑91 (MQ=255)
tCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGCAACTTCa > 1:408820/1‑91 (MQ=255)
ccAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCAc < 1:1018508/91‑1 (MQ=255)
cGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAgg > 2:11410/1‑91 (MQ=255)
gTCGGGTTGATGCGCGAGCGACTTTCCGCTCCTTCCTCGCGCGAACCATCAAGCCCTTTCCCCAGCAGCAGCGGGAAATTCACCGCCAGGt > 2:394893/1‑91 (MQ=255)
gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGGGGGAAATTCACCGCCAGGt > 1:1055065/1‑91 (MQ=255)
gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGt > 1:503408/1‑91 (MQ=255)
gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGt > 1:1056354/1‑91 (MQ=255)
ggTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTgcgc > 1:569291/1‑91 (MQ=255)
gATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCg > 1:402305/1‑91 (MQ=255)
gATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCg > 1:743180/1‑91 (MQ=255)
tGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCCGGTGCGCATCGcc > 2:885442/1‑91 (MQ=255)
gcgagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGa > 2:673326/1‑91 (MQ=255)
gcgagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGa < 2:410195/91‑1 (MQ=255)
gcgagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGa < 2:385349/91‑1 (MQ=255)
gcgagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGa < 1:752496/91‑1 (MQ=255)
gagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAgg > 1:1062651/1‑91 (MQ=255)
aCTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCg > 2:947724/1‑91 (MQ=255)
tCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCtt < 2:1074184/91‑1 (MQ=255)
tCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCtt > 1:767214/1‑91 (MQ=255)
gcgcAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATg > 2:166490/1‑91 (MQ=255)
gACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTcc > 1:149147/1‑91 (MQ=255)
cTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCcagcag > 2:764655/1‑91 (MQ=255)
cTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCcagcag < 2:92573/91‑1 (MQ=255)
tttcCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGAc < 1:539055/91‑1 (MQ=255)
|
CTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGAC > NZ_CP009273/857454‑857626
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTC > NZ_CP009273/857448‑857636
|
GAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCA > SRR3722125.94318/1‑101 (MQ=60)
AATTGCTCAGCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGTAG < SRR3722125.367004/101‑1 (MQ=60)
ATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGC > SRR3722125.962547/1‑101 (MQ=60)
TGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGG > SRR3722125.51814/1‑101 (MQ=60)
CTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGA < SRR3722125.528993/101‑1 (MQ=60)
GTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCA > SRR3722125.201006/1‑101 (MQ=60)
GTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCA > SRR3722125.476762/1‑101 (MQ=60)
GTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGCAACTTCA > SRR3722125.417430/1‑101 (MQ=60)
TTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGGGGGAAATTCACCGCCAGGT > SRR3722125.1078800/1‑101 (MQ=60)
TTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGT > SRR3722125.514057/1‑101 (MQ=60)
TTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGT > SRR3722125.1080123/1‑101 (MQ=60)
CCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGC < SRR3722125.1041324/101‑1 (MQ=60)
AGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGC > SRR3722125.581462/1‑101 (MQ=60)
CCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCG > SRR3722125.410759/1‑101 (MQ=60)
CCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCG > SRR3722125.759212/1‑101 (MQ=60)
GTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGG > SRR3722125.1086574/1‑101 (MQ=60)
GCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTG < SRR3722125.768754/101‑1 (MQ=60)
TTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTT > SRR3722125.783864/1‑101 (MQ=60)
TCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCC > SRR3722125.151982/1‑101 (MQ=60)
TTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTC < SRR3722125.550566/101‑1 (MQ=60)
|
GAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTC > NZ_CP009273/857448‑857636
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
