Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,011,464 |
T→C |
I155V (ATC→GTC) |
fabA ← |
bifunctional 3‑hydroxydecanoyl‑ACP dehydratase/trans‑2‑decenoyl‑ACP isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,011,464 | 0 | T | C | 100.0%
| 173.7
/ NA
| 46 | I155V (ATC→GTC) | fabA | bifunctional 3‑hydroxydecanoyl‑ACP dehydratase/trans‑2‑decenoyl‑ACP isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/24); total (22/24) |
TCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGA > NZ_CP009273/1011379‑1011540
|
tCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAgac < 1:1114068/91‑1 (MQ=255)
tttGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCAt < 1:123716/91‑1 (MQ=255)
tttGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCAt < 2:1095920/91‑1 (MQ=255)
tttGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCAt < 1:141396/91‑1 (MQ=255)
tACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAAcc < 2:1075001/91‑1 (MQ=255)
ctctGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAg > 2:310047/1‑91 (MQ=255)
gtctgATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACt > 2:229253/1‑91 (MQ=255)
ttATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCa > 2:187017/1‑91 (MQ=255)
tataATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCAt > 1:774507/1‑91 (MQ=255)
tataATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCAt < 1:388504/91‑1 (MQ=255)
aaTCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCg > 1:797735/1‑91 (MQ=255)
aaTCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCg > 1:719250/1‑91 (MQ=255)
aGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAg < 1:353922/91‑1 (MQ=255)
aGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAg > 2:74409/1‑91 (MQ=255)
gAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg > 2:144436/1‑91 (MQ=255)
gAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg > 1:69429/1‑91 (MQ=255)
gAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg > 1:69428/1‑91 (MQ=255)
gAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg > 1:210630/1‑91 (MQ=255)
ggCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGccc < 1:24142/91‑1 (MQ=255)
cAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCAt < 1:368357/91‑1 (MQ=255)
aGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATa < 2:956590/91‑1 (MQ=255)
aTCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAga > 2:1088817/1‑91 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac < 1:317390/91‑1 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac < 2:760461/91‑1 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac < 2:839886/91‑1 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac > 2:856207/1‑91 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac < 1:187017/91‑1 (MQ=255)
tCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgac < 1:123924/91‑1 (MQ=255)
ccTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgacg < 2:126492/91‑1 (MQ=255)
cTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgacga < 1:1098890/91‑1 (MQ=255)
aCAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGtt > 2:582941/1‑91 (MQ=255)
gACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGccc > 2:594349/1‑71 (MQ=255)
gACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGccc < 1:594349/71‑1 (MQ=255)
gACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAc > 2:137126/1‑91 (MQ=255)
gACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAc > 1:861529/1‑91 (MQ=255)
gACCGACTTTCAGGTCGCCGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAc > 1:261166/1‑91 (MQ=255)
cGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAAt < 2:378161/91‑1 (MQ=255)
cGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAAt < 2:702913/91‑1 (MQ=255)
ttCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGtt > 2:1068721/1‑91 (MQ=255)
cAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTa < 1:189998/91‑1 (MQ=255)
ggTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTaaa < 1:1068721/91‑1 (MQ=255)
gTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAg < 2:721439/91‑1 (MQ=255)
cGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTg < 2:44830/91‑1 (MQ=255)
gCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGa > 2:1108854/1‑91 (MQ=255)
gCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGa > 1:269949/1‑91 (MQ=255)
gCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGa > 2:872992/1‑91 (MQ=255)
|
TCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGA > NZ_CP009273/1011379‑1011540
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTT > NZ_CP009273/1011364‑1011557
|
CGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGAC < SRR3722125.742220/101‑1 (MQ=60)
GCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACG < SRR3722125.941399/101‑1 (MQ=60)
TCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAAC < SRR3722125.1139212/101‑1 (MQ=60)
TTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCAC < SRR3722125.126048/101‑1 (MQ=60)
TTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCAC < SRR3722125.144090/101‑1 (MQ=60)
TCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCAT > SRR3722125.791338/1‑101 (MQ=60)
GTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCG > SRR3722125.734766/1‑101 (MQ=60)
GTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCG > SRR3722125.815154/1‑101 (MQ=60)
ATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGG > SRR3722125.214796/1‑101 (MQ=60)
ATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGG > SRR3722125.70715/1‑101 (MQ=60)
AATATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGG > SRR3722125.70716/1‑101 (MQ=60)
TATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCC < SRR3722125.396642/101‑1 (MQ=60)
AGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATC < SRR3722125.361321/101‑1 (MQ=60)
taagagacagTCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGAC < SRR3722125.607130/91‑1 (MQ=60)
GGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGAC < SRR3722125.24565/101‑1 (MQ=60)
CAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGA < SRR3722125.376078/101‑1 (MQ=60)
TCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAGC < SRR3722125.126259/101‑1 (MQ=60)
TCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAC < SRR3722125.323917/101‑1 (MQ=60)
TCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAC > SRR3722125.880533/1‑101 (MQ=60)
TCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAC < SRR3722125.190647/101‑1 (MQ=60)
TCCTGGAACAGACCGACTTTCAGGTCGCCGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAAC > SRR3722125.266417/1‑101 (MQ=60)
CTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAA < SRR3722125.1123640/101‑1 (MQ=60)
gagatgtgtataagagacaGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAG < SRR3722125.850449/82‑1 (MQ=60)
CTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGA > SRR3722125.275384/1‑101 (MQ=60)
CAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATAC < SRR3722125.193709/101‑1 (MQ=60)
GGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGG < SRR3722125.1092811/101‑1 (MQ=60)
GTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTT > SRR3722125.345897/1‑101 (MQ=60)
|
CGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTT > NZ_CP009273/1011364‑1011557
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
