Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I184 R1	278	29.6	1166432	97.3%	1134938	124.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	453,912	A→G	E344G (GAA→GGA)	clpX →	ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	453,912	0	A	G	100.0%	116.7 / NA	33	E344G (GAA→GGA)	clpX	ATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

ATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGA  >  NZ_CP009273/453794‑454038

aTTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTcc                                                                                                            >  1:394009/1‑139 (MQ=255)

      cgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGAc                                                                                                       >  2:435344/1‑138 (MQ=255)

                     gCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCt                                                                                        <  1:435344/138‑1 (MQ=255)

                             gaatgaaCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCGCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTaa                                                                                <  2:413125/138‑1 (MQ=255)

                                      gAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg                                                                      >  2:113274/1‑139 (MQ=255)

                                      gAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg                                                                      >  1:488657/1‑139 (MQ=255)

                                       aGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATgg                                                                     >  1:233926/1‑139 (MQ=255)

                                         cGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGa                                                                                            <  1:108302/114‑1 (MQ=255)

                                         cGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGa                                                                                            >  2:108302/1‑114 (MQ=255)

                                            agaagCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGcgcg                                                                 >  1:267227/1‑138 (MQ=255)

                                                           gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGt                                                 <  2:488735/139‑1 (MQ=255)

                                                                 cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGCTCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg                                           >  2:571739/1‑139 (MQ=255)

                                                                 cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATgg                                                                     >  2:529436/1‑113 (MQ=255)

                                                                 cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATgg                                                                     <  1:529436/113‑1 (MQ=255)

                                                                          gAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATc                                  >  2:368338/1‑139 (MQ=255)

                                                                                       aCCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                      <  1:368338/138‑1 (MQ=255)

                                                                                       aCCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                      <  2:4990/138‑1 (MQ=255)

                                                                                       aCCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                      <  2:267227/138‑1 (MQ=255)

                                                                                        ccAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACt                    <  1:5674/139‑1 (MQ=255)

                                                                                           aGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCt                 <  1:193778/139‑1 (MQ=255)

                                                                                            gCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGAc                                                                                                       >  1:502101/1‑52 (MQ=255)

                                                                                            gCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGAc                                                                                                       <  2:502101/52‑1 (MQ=255)

                                                                                             cAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCg               <  2:394009/139‑1 (MQ=255)

                                                                                                tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATa            <  2:233926/139‑1 (MQ=255)

                                                                                                tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATa            <  1:113274/139‑1 (MQ=255)

                                                                                                     ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGa              <  2:35433/132‑1 (MQ=255)

                                                                                                     ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGa              >  1:35433/1‑132 (MQ=255)

                                                                                                       cgcTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa                                                               <  2:368568/81‑1 (MQ=255)

                                                                                                       cgcTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa                                                               >  1:368568/1‑81 (MQ=255)

                                                                                                          tGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGa  >  1:294185/1‑139 (MQ=255)

                                                                                                          tGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGa  >  1:131425/1‑139 (MQ=255)

                                                                                                                aTCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAc           >  1:433608/1‑124 (MQ=255)

                                                                                                                aTCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAc           <  2:433608/124‑1 (MQ=255)

ATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGA  >  NZ_CP009273/453794‑454038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGA  >  NZ_CP009273/453766‑454053

GATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGG                                                                                                                                            <  SRR3722139.164703/150‑1 (MQ=60)

                 TCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCG                                                                                                                          >  SRR3722139.398693/1‑151 (MQ=60)

                                           gtgtataagagacaGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTA                                                                                                <  SRR3722139.109627/137‑1 (MQ=60)

                                                CGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAA                                                                                            <  SRR3722139.440468/150‑1 (MQ=60)

                                                       TTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGG                                                                                    >  SRR3722139.494333/1‑151 (MQ=60)

                                                        TGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGC                                                                                   >  SRR3722139.236811/1‑151 (MQ=60)

                                                             GAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGT                                                                               >  SRR3722139.270630/1‑150 (MQ=60)

                                                                   gtgtataagagacaGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACC                                                                         <  SRR3722139.535531/136‑1 (MQ=60)

                                                                                                   agtacggtctcgtgggctcggagatgtgtataagagacagAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAG                                             <  SRR3722139.121014/106‑1 (MQ=60)

                                                                                                             GCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGActgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttgaaaaaaaaaa                               >  SRR3722139.507901/1‑74 (MQ=60)

                                                                                                                 cggagatgtgtataagagacagGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAC                          <  SRR3722139.459495/129‑1 (MQ=60)

                                                                                                                  GACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAC                          <  SRR3722139.372753/150‑1 (MQ=60)

                                                                                                                   ACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCA                        <  SRR3722139.5729/151‑1 (MQ=60)

                                                                                                                      AAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGT                     >  SRR3722139.35816/1‑151 (MQ=60)

                                                                                                                      AAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGT                     <  SRR3722139.196166/151‑1 (MQ=60)

                                                                                                                        GCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCtgtctcttatacacatctccgagcccacgagaccgtactagatctcg                   >  SRR3722139.372985/1‑104 (MQ=60)

                                                                                                                           GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAT                >  SRR3722139.297878/1‑151 (MQ=60)

                                                                                                                           GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAT                >  SRR3722139.133033/1‑151 (MQ=60)

                                                                                                                           GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAT                <  SRR3722139.114638/151‑1 (MQ=60)

                                                                                                                                 GGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCctgtc          >  SRR3722139.438687/1‑146 (MQ=60)

                                                                                                                                     CTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCC       >  SRR3722139.26486/1‑150 (MQ=60)

                                                                                                                                       GTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgt    >  SRR3722139.473706/1‑95 (MQ=60)

                                                                                                                                          TAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGA  <  SRR3722139.322640/150‑1 (MQ=60)

GATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGA  >  NZ_CP009273/453766‑454053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`