Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I88 R1
|
217 |
30.5 |
1742422 |
95.9% |
1670982 |
87.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
453,912 |
A→G |
E344G (GAA→GGA) |
clpX → |
ATP‑dependent protease ATP‑binding subunit ClpX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 453,912 | 0 | A | G | 100.0%
| 112.7
/ NA
| 30 | E344G (GAA→GGA) | clpX | ATP‑dependent protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/12); total (18/12) |
AACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCC > NZ_CP009273/453828‑453995
|
aaCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGt < 2:36717/91‑1 (MQ=255)
tGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGa < 2:500283/91‑1 (MQ=255)
tGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGa < 2:332787/91‑1 (MQ=255)
gaagaaGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTgg < 1:186337/91‑1 (MQ=255)
aagaagCTCTGATTGAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGa > 1:746192/1‑91 (MQ=255)
gCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTcc > 1:219371/1‑91 (MQ=255)
gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcg > 1:107125/1‑91 (MQ=255)
gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcg > 2:493605/1‑91 (MQ=255)
aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcgc < 2:815115/91‑1 (MQ=255)
cTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTgg > 2:75025/1‑91 (MQ=255)
cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGAt < 1:594207/91‑1 (MQ=255)
aaaacCGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTa < 1:855004/91‑1 (MQ=255)
gACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg > 2:510446/1‑91 (MQ=255)
ccAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgc < 2:499747/91‑1 (MQ=255)
cAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa < 1:165849/91‑1 (MQ=255)
gTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaa > 2:848778/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 1:72233/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 2:382511/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 2:431501/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 1:594879/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 1:216362/1‑91 (MQ=255)
tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa > 2:868057/1‑91 (MQ=255)
ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGt < 1:868057/91‑1 (MQ=255)
ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGt > 1:433542/1‑91 (MQ=255)
cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc > 2:340469/1‑91 (MQ=255)
cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc > 1:625349/1‑91 (MQ=255)
cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc > 1:238026/1‑91 (MQ=255)
gTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGt > 1:827088/1‑91 (MQ=255)
tttAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTg < 1:300635/91‑1 (MQ=255)
aaTCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGcc < 1:497426/91‑1 (MQ=255)
|
AACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCC > NZ_CP009273/453828‑453995
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAA > NZ_CP009273/453813‑454012
|
TCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGA > SRR3722118.6450/1‑101 (MQ=60)
GAACTGAGCGAAGAAGCTCTGATTGAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGA > SRR3722118.762590/1‑101 (MQ=60)
GAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCC > SRR3722118.223781/1‑101 (MQ=60)
GAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGA < SRR3722118.190021/101‑1 (MQ=60)
CTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCG > SRR3722118.109097/1‑101 (MQ=60)
CAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTA < SRR3722118.606841/101‑1 (MQ=60)
AAAACCGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGAT < SRR3722118.873979/101‑1 (MQ=60)
GACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAA > SRR3722118.220706/1‑101 (MQ=60)
GACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAA > SRR3722118.73574/1‑101 (MQ=60)
GACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAA > SRR3722118.607527/1‑101 (MQ=60)
AGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGT > SRR3722118.442693/1‑101 (MQ=60)
CAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGC < SRR3722118.169090/101‑1 (MQ=60)
GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTctgtctcttatacacatctccgagcccacgagacggactcctatctcgtatgccgtcttctgctt > SRR3722118.320720/1‑36 (MQ=60)
GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCC > SRR3722118.242836/1‑101 (MQ=60)
GTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCC > SRR3722118.638789/1‑101 (MQ=60)
ATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGT > SRR3722118.845315/1‑101 (MQ=60)
GGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGCCC < SRR3722118.887392/101‑1 (MQ=60)
GTTTAATCTGGGAGGGGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTT > SRR3722118.547238/1‑101 (MQ=60)
GTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTT > SRR3722118.761666/1‑101 (MQ=60)
GTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTT > SRR3722118.316582/1‑101 (MQ=60)
TTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTC < SRR3722118.306810/101‑1 (MQ=60)
AATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAT < SRR3722118.507976/101‑1 (MQ=60)
GAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAA < SRR3722118.614907/101‑1 (MQ=60)
|
TCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAA > NZ_CP009273/453813‑454012
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
