Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F4 I96 R1	227	23.3	882352	98.0%	864704	126.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,629,926	C→T	A66A (GCG→GCA)	arcA ←	two‑component system response regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,629,926	0	C	T	100.0%	140.9 / NA	35	A66A (GCG→GCA)	arcA	two‑component system response regulator ArcA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/17); total (18/17)

TCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCC  >  NZ_CP009273/4629790‑4630049

tCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACgtgc                                                                                                                           <  2:228498/139‑1 (MQ=255)

          aCGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACagaag                                                                                                                  <  1:30452/138‑1 (MQ=255)

           cgcgGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGAc                                                                                                                <  1:45446/139‑1 (MQ=255)

                 ttGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCt                                                                                                          >  2:15109/1‑139 (MQ=255)

                          ttGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCgg                                                                                                   >  2:96377/1‑137 (MQ=255)

                          ttGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCgg                                                                                                   >  2:347589/1‑137 (MQ=255)

                             gTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGa                                                                                               <  1:433807/138‑1 (MQ=255)

                                   tAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGAt                                                                                              >  2:130831/1‑133 (MQ=255)

                                   tAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGAt                                                                                              <  1:130831/133‑1 (MQ=255)

                                   tAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGatat                                                                                        >  1:62478/1‑139 (MQ=255)

                                   tAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGatat                                                                                        >  2:359436/1‑139 (MQ=255)

                                       cATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCAt                                                                                    >  2:75643/1‑139 (MQ=255)

                                       cATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCAt                                                                                    >  1:248078/1‑139 (MQ=255)

                                        aTCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAg                                                                                                >  2:147094/1‑126 (MQ=255)

                                        aTCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAg                                                                                                <  1:147094/126‑1 (MQ=255)

                                           tGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGAt                                                                                 >  2:367160/1‑138 (MQ=255)

                                                cGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAg                                                                           >  1:56925/1‑139 (MQ=255)

                                                cGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAg                                                                           >  2:109656/1‑139 (MQ=255)

                                                 gATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAgg                                                                          <  1:142985/139‑1 (MQ=255)

                                                  aTTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGt                                                                         <  1:420372/139‑1 (MQ=255)

                                                  aTTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGt                                                                         >  1:255072/1‑139 (MQ=255)

                                                  aTTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGt                                                                         >  2:294962/1‑139 (MQ=255)

                                                       gAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATg                                                                    <  1:368128/139‑1 (MQ=255)

                                                         ggCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTc                                                                  <  1:352301/139‑1 (MQ=255)

                                                                 aTTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCag                                                          <  1:256791/139‑1 (MQ=255)

                                                                    ttATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagag                                                        <  2:132528/138‑1 (MQ=255)

                                                                    ttATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagag                                                        <  2:62478/138‑1 (MQ=255)

                                                                    ttATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagag                                                        <  1:89952/138‑1 (MQ=255)

                                                                     tATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagaga                                                       <  1:131610/138‑1 (MQ=255)

                                                                      aTCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagagag                                                      <  1:187422/138‑1 (MQ=255)

                                                                                             tCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATc                              >  1:417194/1‑139 (MQ=255)

                                                                                                       cAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATg                                             <  2:210023/114‑1 (MQ=255)

                                                                                                       cAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATg                                             >  1:210023/1‑114 (MQ=255)

                                                                                                                aTTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatca           <  1:294962/139‑1 (MQ=255)

                                                                                                                    gCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGc       >  2:22363/1‑139 (MQ=255)

                                                                                                                         cTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTcc  >  2:116867/1‑139 (MQ=255)

TCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCC  >  NZ_CP009273/4629790‑4630049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGC  >  NZ_CP009273/4629778‑4630051

agtctcgtgggctcggagatgtgtataagagacagGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTG                                                                                                                              <  SRR3722119.362654/115‑1 (MQ=60)

 GCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGC                                                                                                                             <  SRR3722119.354267/150‑1 (MQ=60)

                     CACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTAC                                                                                                         <  SRR3722119.30716/150‑1 (MQ=60)

                      ACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCC                                                                                                       <  SRR3722119.45846/151‑1 (MQ=60)

                                    GTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATC                                                                                         >  SRR3722119.63058/1‑151 (MQ=60)

                                      caGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCA                                                                                       <  SRR3722119.148601/149‑1 (MQ=60)

                                        GGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATG                                                                                     <  SRR3722119.132153/151‑1 (MQ=60)

                                        GGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATG                                                                                     >  SRR3722119.250654/1‑151 (MQ=60)

                                        GGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCAT                                                                                      <  SRR3722119.438147/150‑1 (MQ=60)

                                                 GTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGG                                                                            >  SRR3722119.57454/1‑151 (MQ=60)

                                                   CATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTT                                                                          >  SRR3722119.257727/1‑151 (MQ=60)

                                                            CGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATA                                                                 <  SRR3722119.144466/151‑1 (MQ=60)

                                                             GATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCAGAT                                                                <  SRR3722119.424591/151‑1 (MQ=60)

                                                                  CGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGA                                                           <  SRR3722119.371848/151‑1 (MQ=60)

                                                                    AGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGA                                                         <  SRR3722119.355881/151‑1 (MQ=60)

                                                                            AATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGA                                                 <  SRR3722119.259459/151‑1 (MQ=60)

                                                                               TTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATG                                               <  SRR3722119.90834/150‑1 (MQ=60)

                                                                                TTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGC                                              <  SRR3722119.132947/150‑1 (MQ=60)

                                                                                 TATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCA                                             <  SRR3722119.189385/150‑1 (MQ=60)

                                                                                              GTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCT                               >  SRR3722119.421391/1‑151 (MQ=60)

                                                                                                        GTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCtgtctcttatacac                     >  SRR3722119.212279/1‑137 (MQ=60)

                                                                                                                           CATTCGCCTGCTCGCGCAGTTCACGTGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGC  <  SRR3722119.297974/151‑1 (MQ=60)

TGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGC  >  NZ_CP009273/4629778‑4630051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`