Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I178 R1
|
167 |
28.0 |
1137820 |
95.3% |
1084342 |
115.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
557,194 |
A→G |
Q681R (CAG→CGG) |
sfmD → |
fimbrial biogenesis usher protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 557,194 | 0 | A | G | 100.0%
| 61.5
/ NA
| 18 | Q681R (CAG→CGG) | sfmD | fimbrial biogenesis usher protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/5); total (13/5) |
AGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGA > NZ_CP009273/557061‑557326
|
aGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCt < 2:234453/137‑1 (MQ=255)
aTCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTgg > 2:351497/1‑139 (MQ=255)
cGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATgg > 2:27631/1‑139 (MQ=255)
cacGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagc > 1:525285/1‑139 (MQ=255)
acGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGagcc < 2:525285/139‑1 (MQ=255)
ggCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGa > 1:27562/1‑138 (MQ=255)
cTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGag < 2:102659/106‑1 (MQ=255)
cTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGag > 1:102659/1‑106 (MQ=255)
gggggCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAg < 2:208283/139‑1 (MQ=255)
ggCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAACCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCg > 2:397620/1‑139 (MQ=255)
ggTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGgtg > 1:384945/1‑138 (MQ=255)
gACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTaa > 1:307822/1‑107 (MQ=255)
gACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTaa < 2:307822/107‑1 (MQ=255)
gACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaa > 1:143217/1‑138 (MQ=255)
aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 1:308959/1‑139 (MQ=255)
aCCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGaaaa > 1:190018/1‑139 (MQ=255)
cgATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCa > 1:35414/1‑138 (MQ=255)
aCTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGa > 2:239974/1‑139 (MQ=255)
|
AGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGA > NZ_CP009273/557061‑557326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCG > NZ_CP009273/557091‑557332
|
AATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCC > SRR3722132.534582/1‑151 (MQ=60)
GGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGAT > SRR3722132.28042/1‑150 (MQ=60)
CTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGctgtctcttatacacatctccg > SRR3722132.104574/1‑128 (MQ=60)
GCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGT > SRR3722132.391961/1‑150 (MQ=60)
GGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCtgtctcttatacacatctccg > SRR3722132.313610/1‑130 (MQ=60)
GGTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAA > SRR3722132.145861/1‑150 (MQ=60)
GTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAAT > SRR3722132.193581/1‑151 (MQ=60)
GTATAACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAAT > SRR3722132.314762/1‑151 (MQ=60)
AACTACGACCGCGATCAACATGACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAA > SRR3722132.36030/1‑150 (MQ=60)
ACGTTAACTGGCGGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCG > SRR3722132.187442/1‑151 (MQ=60)
|
AATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAATCAAACTGGCATTTTAACCGACTGGCG > NZ_CP009273/557091‑557332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
