Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I178 R1
|
167 |
28.0 |
1137820 |
95.3% |
1084342 |
115.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
609,496 |
A→C |
L33F (TTA→TTC) |
ybdZ → |
enterobactin biosynthesis protein YbdZ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 609,496 | 0 | A | C | 100.0%
| 91.2
/ NA
| 25 | L33F (TTA→TTC) | ybdZ | enterobactin biosynthesis protein YbdZ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (12/13); total (12/13) |
CCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAT > NZ_CP009273/609362‑609614
|
ccTCTGGCAACCCCTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg < 2:262890/139‑1 (MQ=255)
gCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCt < 2:56029/139‑1 (MQ=255)
ggCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCg > 2:129873/1‑139 (MQ=255)
ggCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCg > 2:505973/1‑139 (MQ=255)
gCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGt < 2:479331/139‑1 (MQ=255)
tCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTg > 1:327112/1‑139 (MQ=255)
tCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTg > 1:401911/1‑139 (MQ=255)
aTCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcagca < 1:129873/139‑1 (MQ=255)
tCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCt > 2:369755/1‑139 (MQ=255)
atCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg > 2:131896/1‑78 (MQ=255)
atCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg < 1:131896/78‑1 (MQ=255)
tCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGcc < 2:401911/139‑1 (MQ=255)
ccGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAg < 1:520715/105‑1 (MQ=255)
ccGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAg > 2:520715/1‑105 (MQ=255)
cAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACt > 2:124467/1‑139 (MQ=255)
gAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCg < 1:415061/139‑1 (MQ=255)
aGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcag < 1:234931/115‑1 (MQ=255)
aGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcag > 2:234931/1‑115 (MQ=255)
gcgcAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACcgac > 2:282233/1‑138 (MQ=255)
gcAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc < 1:124467/139‑1 (MQ=255)
aGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCaggagg < 2:450642/139‑1 (MQ=255)
aGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCaggagg < 1:369755/139‑1 (MQ=255)
gTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc < 2:386317/125‑1 (MQ=255)
gTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc > 1:386317/1‑125 (MQ=255)
ggCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAt > 1:166920/1‑138 (MQ=255)
|
CCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAT > NZ_CP009273/609362‑609614
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAG > NZ_CP009273/609361‑609645
|
atacgagatctagtacggtctcgtgggctcggagatgtgtataagagacagTCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGAC < SRR3722132.134338/100‑1 (MQ=60)
cggagatgtgtataagagacagCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTC < SRR3722132.529944/127‑1 (MQ=60)
GAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGC > SRR3722132.333219/1‑151 (MQ=60)
GAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGC > SRR3722132.409187/1‑151 (MQ=60)
gtataagagacagGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGA < SRR3722132.239337/137‑1 (MQ=60)
AATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAG < SRR3722132.132287/151‑1 (MQ=60)
GGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACAC < SRR3722132.422579/151‑1 (MQ=60)
CGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGA < SRR3722132.126799/151‑1 (MQ=60)
GGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGActgt > SRR3722132.393347/1‑147 (MQ=60)
ATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATG > SRR3722132.170062/1‑150 (MQ=60)
CAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCC < SRR3722132.376525/151‑1 (MQ=60)
TCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaacaaataagcgtaattataaactctgc > SRR3722132.394518/1‑43 (MQ=60)
|
ACCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAATGAGCCAGCATTTACCTTTGGTCGCCGCACAG > NZ_CP009273/609361‑609645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
