Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I178 R1
|
167 |
28.0 |
1137820 |
95.3% |
1084342 |
115.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,015,913 |
T→G |
T155P (ACG→CCG) |
sulA ← |
cell division inhibitor SulA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,015,913 | 0 | T | G | 100.0%
| 57.1
/ NA
| 17 | T155P (ACG→CCG) | sulA | cell division inhibitor SulA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (8/9); total (8/9) |
GCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACA > NZ_CP009273/1015829‑1016037
|
gCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGc < 1:405226/88‑1 (MQ=255)
gCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGc > 2:405226/1‑88 (MQ=255)
gCGACAAAAAAAGATCCAGTATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGATGCATAATAAACCCCATAGCGTTACCtt > 2:196567/1‑138 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCa < 2:293621/138‑1 (MQ=255)
aTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAg > 1:215275/1‑138 (MQ=255)
aaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc < 1:233675/95‑1 (MQ=255)
aaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc > 2:233675/1‑95 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTAcc > 1:32982/1‑72 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTAcc < 2:32982/72‑1 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATg < 2:79614/131‑1 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATg > 1:79614/1‑131 (MQ=255)
cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCataat > 2:359164/1‑72 (MQ=255)
cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCataat < 1:359164/72‑1 (MQ=255)
aaTTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGccaaccaa < 2:140045/139‑1 (MQ=255)
ttAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGa < 2:215275/138‑1 (MQ=255)
ccGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATcacca > 2:433222/1‑139 (MQ=255)
ggAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACcaca < 2:405991/139‑1 (MQ=255)
|
GCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACA > NZ_CP009273/1015829‑1016037
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGC > NZ_CP009273/1015772‑1016053
|
ACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGA < SRR3722132.486045/151‑1 (MQ=60)
CCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAG < SRR3722132.194660/151‑1 (MQ=60)
GCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGG > SRR3722132.390890/1‑150 (MQ=60)
agtacggtctcgtgggctcggagatgtgtataagagacagGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATG < SRR3722132.412589/110‑1 (MQ=60)
gacggcatacgagatctagtacggtctcgtgggctcggagatgtgtataagagacagACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAG < SRR3722132.365774/94‑1 (MQ=60)
gtctcgtgggctcggagatgtgtataagagaCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACC < SRR3722132.238053/120‑1 (MQ=60)
TTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGC > SRR3722132.219290/1‑150 (MQ=60)
ATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtc > SRR3722132.33562/1‑94 (MQ=60)
ATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTC > SRR3722132.81057/1‑151 (MQ=60)
GTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttgaa > SRR3722132.221998/1‑82 (MQ=60)
|
ACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGC > NZ_CP009273/1015772‑1016053
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
