Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I179 R1
|
182 |
40.3 |
1547118 |
96.8% |
1497610 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,015,913 |
T→G |
T155P (ACG→CCG) |
sulA ← |
cell division inhibitor SulA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,015,913 | 0 | T | G | 100.0%
| 81.6
/ NA
| 24 | T155P (ACG→CCG) | sulA | cell division inhibitor SulA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/11); total (13/11) |
AGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACA > NZ_CP009273/1015780‑1016037
|
aGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGt > 1:403837/1‑139 (MQ=255)
gCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGagag > 1:117136/1‑138 (MQ=255)
gCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGagag > 2:570885/1‑138 (MQ=255)
aCGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCataat < 2:117136/139‑1 (MQ=255)
aaGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa < 2:403837/139‑1 (MQ=255)
tGGCTGGGCGATAAAAAAAGTTCCAGGCTTAATCCTAAATTTACTTAATGATACACATTAGTGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGTCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 2:184152/1‑139 (MQ=255)
tGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:461373/1‑139 (MQ=255)
gCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCtt > 1:398105/1‑138 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACg < 1:485863/89‑1 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACg > 2:485863/1‑89 (MQ=255)
aTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc < 1:208237/101‑1 (MQ=255)
aTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc > 2:208237/1‑101 (MQ=255)
aaaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGct > 1:523737/1‑139 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGc < 1:694352/128‑1 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGc > 2:694352/1‑128 (MQ=255)
aCTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTcttctt > 2:230508/1‑139 (MQ=255)
aTGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTc < 1:575311/139‑1 (MQ=255)
gagTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCataat < 1:99517/65‑1 (MQ=255)
gagTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCataat > 2:99517/1‑65 (MQ=255)
gagTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGcca < 2:398105/139‑1 (MQ=255)
aaTTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGccaaccaa < 2:507545/139‑1 (MQ=255)
ggAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATca > 1:558548/1‑134 (MQ=255)
ggAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATca < 2:558548/134‑1 (MQ=255)
ggAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACcaca < 2:91735/139‑1 (MQ=255)
|
AGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACA > NZ_CP009273/1015780‑1016037
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GAAACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGCGTA > NZ_CP009273/1015769‑1016056
|
GAAACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTG > SRR3722133.409429/1‑151 (MQ=60)
GCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGG > SRR3722133.118702/1‑150 (MQ=60)
agtacggtctcgtgggctcggagatgtgtataagagacagGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACC < SRR3722133.492422/111‑1 (MQ=60)
agcagaagacggcatacgagatctagtacggtctcgtgggctcggagatgtgtataagagacagGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAG < SRR3722133.100814/87‑1 (MQ=60)
ACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT > SRR3722133.467640/1‑151 (MQ=60)
gggctcggagatgtgtataagagacAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACC < SRR3722133.211015/125‑1 (MQ=60)
GCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTC > SRR3722133.403636/1‑150 (MQ=60)
GGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTC > SRR3722133.530779/1‑151 (MQ=60)
GATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTT < SRR3722133.703361/151‑1 (MQ=60)
AATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGC < SRR3722133.582955/151‑1 (MQ=60)
ATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACAC > SRR3722133.565986/1‑151 (MQ=60)
GTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCtgtctcttatacacatctccgagcccacg > SRR3722133.338974/1‑121 (MQ=60)
|
GAAACCGCTTCAGACAAGCCTCCGCAAGGAAAATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGCGTA > NZ_CP009273/1015769‑1016056
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
