Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 368 332.2 13092066 97.0% 12699304 150.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,537,108 A→G Y105Y (TAT→TAC)  RPPX_20270 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,537,1080AG100.0% 297.8 / NA 94Y105Y (TAT→TAC) RPPX_20270hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (59/35);  total (59/35)

TGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536961‑4537159
                                                                                                                                                   |                                                   
tggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAg                                                  <  1:6388461/151‑1 (MQ=255)
 ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 >  2:1603567/1‑151 (MQ=255)
 ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                 <  2:386974/151‑1 (MQ=255)
  gcgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTg                                                >  2:3324275/1‑151 (MQ=255)
   cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               >  1:6099255/1‑151 (MQ=255)
   cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                               >  1:4178720/1‑151 (MQ=255)
    gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgc                                              >  2:4416064/1‑151 (MQ=255)
     ctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcg                                             >  1:3624603/1‑151 (MQ=255)
      tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                            >  2:2767117/1‑151 (MQ=255)
      tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                            >  2:305798/1‑151 (MQ=255)
       gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACATCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:1961802/1‑151 (MQ=255)
       gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCTCGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:2181033/1‑151 (MQ=255)
       gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:2180964/1‑151 (MQ=255)
       gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                           >  1:1963893/1‑151 (MQ=255)
        ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  1:6027716/1‑151 (MQ=255)
        ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  2:1882683/1‑151 (MQ=255)
        ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          >  1:3705077/1‑151 (MQ=255)
        cgcGGACGTTGAACGGGCGGCAGATGCCATACCAGCAGAGGGCGACGGGCAGGGTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                          <  2:5581456/149‑1 (MQ=255)
         tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGTGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTTGCGCCac                                         >  1:944121/1‑150 (MQ=255)
         tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         >  1:1321407/1‑151 (MQ=255)
         tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         <  1:306798/151‑1 (MQ=255)
         tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                         <  1:2643542/151‑1 (MQ=255)
          cGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAgg                                        <  1:4760620/151‑1 (MQ=255)
           ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  1:532067/1‑151 (MQ=255)
           ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  2:3282197/1‑151 (MQ=255)
           ggACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGc                                       >  1:3232876/1‑151 (MQ=255)
             aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCTGGTTCGCCAGGGGGCGTTGTGGCGCCAGGCtt                                     >  2:3530194/1‑151 (MQ=255)
             aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  2:5323806/1‑151 (MQ=255)
             aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  1:3948001/1‑151 (MQ=255)
             aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     >  2:4484851/1‑151 (MQ=255)
             aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                     <  1:3324275/151‑1 (MQ=255)
              cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    <  2:6380046/151‑1 (MQ=255)
              cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    >  1:697124/1‑151 (MQ=255)
              cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                    <  1:5921442/151‑1 (MQ=255)
               gTTGCACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   >  2:5378744/1‑151 (MQ=255)
               gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                   <  1:212838/151‑1 (MQ=255)
                 tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCTTGCATTTCACCGGGCTCGCCCGTTCGTCAGGGGGCGTCGCGGCGGCAGCCTTCTGc                                 >  2:1712980/1‑151 (MQ=17)
                 tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:4979339/1‑151 (MQ=255)
                 tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:1712997/1‑151 (MQ=255)
                 tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                 >  2:1243641/1‑151 (MQ=255)
                   aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                               <  2:3690942/151‑1 (MQ=255)
                    aCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgg                              >  1:5444375/1‑151 (MQ=255)
                      aGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                            <  2:5970663/151‑1 (MQ=255)
                        gcggcgGATGCCATGCCAGCAGAGGGCGGCGGTCAGGTTGTAGGGGAACAGCACGGCCGAGAACGGCAATTGCCGGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtg                          <  2:1329454/151‑2 (MQ=14)
                        gcggcAGCTGCCAGAGCAGCGGGGGGCGACGGGCAGGTTGTAGGGGGACAGCACGGCGCAGAACGGCAATTGCCAGTGCTTTTTGCCCTGCATGTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGGTTCTGCCcggggg                          <  2:5796243/151‑3 (MQ=11)
                         cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         >  1:4877492/1‑149 (MQ=255)
                         cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         <  1:2223176/151‑3 (MQ=255)
                         cggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGtga                         >  1:4384355/1‑149 (MQ=255)
                          ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGTGGTGAg                        >  2:538085/1‑151 (MQ=255)
                          ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAg                        <  1:6298794/151‑1 (MQ=255)
                          ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAg                        >  2:3858373/1‑151 (MQ=255)
                           gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      <  2:2900818/136‑1 (MQ=255)
                           gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                      >  1:2900818/1‑136 (MQ=255)
                           gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                       <  2:643669/151‑1 (MQ=255)
                           gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                       <  1:3079513/151‑1 (MQ=255)
                            cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  1:5139082/151‑1 (MQ=255)
                            cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      >  2:4181657/1‑151 (MQ=255)
                            cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      >  2:2377390/1‑151 (MQ=255)
                            cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                      <  1:3307879/151‑1 (MQ=255)
                             aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:5519146/151‑1 (MQ=255)
                             aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  2:390495/151‑1 (MQ=255)
                             aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                     <  1:2553206/151‑1 (MQ=255)
                              gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  2:1042791/1‑151 (MQ=255)
                              gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  1:3810490/1‑151 (MQ=255)
                              gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                    >  2:1647394/1‑151 (MQ=255)
                               aTGCGATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  2:5382319/1‑151 (MQ=255)
                               aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  1:306132/1‑151 (MQ=255)
                               aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                   >  2:169197/1‑151 (MQ=255)
                                tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGCACAGCGCGGCCCAGGACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  2:1785304/151‑1 (MQ=255)
                                tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  2:1786368/151‑1 (MQ=255)
                                tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                  <  1:1770845/151‑1 (MQ=255)
                                 gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  1:4896614/1‑151 (MQ=255)
                                 gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  1:4896404/1‑151 (MQ=255)
                                 gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 <  2:2857561/151‑1 (MQ=255)
                                 gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                 >  2:2039982/1‑151 (MQ=255)
                                   cATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGc               >  1:6074279/1‑151 (MQ=255)
                                   cATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGc               <  1:3642935/151‑1 (MQ=25)
                                     tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt             >  1:6050660/1‑151 (MQ=255)
                                     tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGACGt             >  2:1765477/1‑151 (MQ=255)
                                      aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg            <  1:4139962/151‑1 (MQ=19)
                                      aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg            >  1:3205579/1‑151 (MQ=21)
                                       ccAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGc           <  1:78710/151‑1 (MQ=255)
                                        cagcagAGGGCGACGGTCAGGTTGCAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCCTCTGCCGGGGTGAGGCAGATCGCGTGcc          >  2:1829986/1‑150 (MQ=11)
                                         agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTGACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGGGCCAGCCTTCTGCCGGGGTGGGGCAGAGCGCGTGccc         >  2:5113493/1‑149 (MQ=11)
                                         agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg         <  1:1748696/151‑1 (MQ=16)
                                           cagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTg       <  2:2048085/151‑1 (MQ=12)
                                             gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     >  2:2251095/1‑151 (MQ=14)
                                             gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc     >  2:2250714/1‑151 (MQ=9)
                                              agGGCGCCGGTCAGGTCGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTGACCGGGCCCGCCTGGCCCCCATGGGGCGTAGCGGCCCCAGTCTTCCGCCGGGAGGCGGCAGCTGCCGCGCAGTgccg    >  2:3745956/1‑148 (MQ=2)
                                              agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt    >  1:5713699/1‑151 (MQ=14)
                                              agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt    >  2:2233030/1‑151 (MQ=14)
                                               gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt   <  1:381167/151‑1 (MQ=9)
                                               gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt   >  1:6469902/1‑151 (MQ=14)
                                                ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg  <  1:3475672/151‑1 (MQ=2)
                                                                                                                                                   |                                                   
TGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTG  >  CP009974/4536961‑4537159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCATGAAGTGGCG  >  CP009974/4536959‑4537221
                                                                                                                                                     |                                                                                                                 
GGTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGT                                                                                                                  >  SRR10918232.4138394/1‑151 (MQ=60)
  TGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAG                                                                                                                <  SRR10918232.6418479/151‑1 (MQ=60)
     CGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGG                                                                                                             >  SRR10918232.6127441/1‑151 (MQ=60)
     CGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGG                                                                                                             >  SRR10918232.4196815/1‑151 (MQ=60)
       CTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCG                                                                                                           >  SRR10918232.3640430/1‑151 (MQ=60)
         GCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACATCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCC                                                                                                         >  SRR10918232.1970356/1‑151 (MQ=60)
         GCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCTCGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCC                                                                                                         >  SRR10918232.2190450/1‑151 (MQ=60)
         GCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCC                                                                                                         >  SRR10918232.2190381/1‑151 (MQ=60)
         GCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGATAGGGGGCGTAGTGGCGCC                                                                                                         >  SRR10918232.1972461/1‑151 (MQ=60)
          CTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCA                                                                                                        >  SRR10918232.6055496/1‑151 (MQ=60)
          CTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCA                                                                                                        >  SRR10918232.3721324/1‑151 (MQ=60)
           TCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGTGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTTGCGCCAC                                                                                                       >  SRR10918232.947814/1‑151 (MQ=60)
           TCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAG                                                                                                       >  SRR10918232.1327070/1‑151 (MQ=60)
           TCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAG                                                                                                       <  SRR10918232.307819/151‑1 (MQ=60)
           TCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAG                                                                                                       <  SRR10918232.2654739/151‑1 (MQ=60)
            CGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGG                                                                                                      <  SRR10918232.4781763/151‑1 (MQ=60)
             GGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGC                                                                                                     >  SRR10918232.533976/1‑151 (MQ=60)
             GGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGC                                                                                                     >  SRR10918232.3247095/1‑151 (MQ=60)
               ACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTT                                                                                                   >  SRR10918232.3965151/1‑151 (MQ=60)
               ACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTT                                                                                                   <  SRR10918232.3338867/151‑1 (MQ=60)
                CGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTC                                                                                                  >  SRR10918232.699646/1‑151 (MQ=60)
                CGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTC                                                                                                  <  SRR10918232.5948606/151‑1 (MQ=60)
                 GTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCT                                                                                                 <  SRR10918232.213537/151‑1 (MQ=60)
                      ACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGG                                                                                            >  SRR10918232.5469184/1‑151 (MQ=57)
                           CGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGA                                                                                       >  SRR10918232.4403477/1‑151 (MQ=46)
                           CGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGA                                                                                       >  SRR10918232.4899368/1‑151 (MQ=46)
                           CGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGA                                                                                       <  SRR10918232.2232721/151‑1 (MQ=46)
                            GGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAG                                                                                      <  SRR10918232.6328163/151‑1 (MQ=43)
                             GCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTagatcggaagagcac                                                                                     >  SRR10918232.2913302/1‑136 (MQ=44)
                             GCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGG                                                                                     <  SRR10918232.3092908/151‑1 (MQ=41)
                              CAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGC                                                                                    <  SRR10918232.3322400/151‑1 (MQ=39)
                              CAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGC                                                                                    <  SRR10918232.5162383/151‑1 (MQ=39)
                               AGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCA                                                                                   <  SRR10918232.2564018/151‑1 (MQ=37)
                                GATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAG                                                                                  >  SRR10918232.3827128/1‑151 (MQ=35)
                                 ATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGA                                                                                 >  SRR10918232.307153/1‑151 (MQ=33)
                                  TGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAT                                                                                <  SRR10918232.1778717/151‑1 (MQ=31)
                                   GCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATG                                                                               >  SRR10918232.4918640/1‑151 (MQ=29)
                                   GCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATG                                                                               >  SRR10918232.4918429/1‑151 (MQ=29)
                                     CATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGC                                                                             <  SRR10918232.3658850/151‑1 (MQ=24)
                                     CATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGC                                                                             >  SRR10918232.6102315/1‑151 (MQ=24)
                                       TACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGT                                                                           >  SRR10918232.6078568/1‑151 (MQ=20)
                                        ACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTG                                                                          <  SRR10918232.4157881/151‑1 (MQ=20)
                                        ACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTG                                                                          >  SRR10918232.3219634/1‑151 (MQ=20)
                                         CCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGC                                                                         <  SRR10918232.78936/151‑1 (MQ=18)
                                           AGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAG                                                                       <  SRR10918232.1756501/151‑1 (MQ=11)
                                                AGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCT                                                                  >  SRR10918232.5739795/1‑151 (MQ=3)
                                                 GGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTT                                                                 >  SRR10918232.6500521/1‑151 (MQ=1)
                                                 GGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTT                                                                 <  SRR10918232.382479/151‑1 (MQ=1)
                                                  GGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTG                                                                <  SRR10918232.3490893/151‑1 (MQ=0)
                                                     GACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGC                                                             >  SRR10918232.908185/1‑151 (MQ=0)
                                                      ACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCC                                                            <  SRR10918232.6108278/151‑1 (MQ=0)
                                                       CGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCA                                                           <  SRR10918232.3505609/151‑1 (MQ=0)
                                                           CAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAG                                                       >  SRR10918232.5571402/1‑151 (MQ=0)
                                                              GTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTG                                                    >  SRR10918232.734649/1‑151 (MQ=0)
                                                                    GGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAagatcggaagagcacacgtctgaact                                              >  SRR10918232.805993/1‑125 (MQ=0)
                                                                        AACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGG                                          <  SRR10918232.2149647/151‑1 (MQ=0)
                                                                        AACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGGGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTAACCGG                                          >  SRR10918232.4476295/1‑151 (MQ=0)
                                                                         ACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGG                                         <  SRR10918232.2252543/151‑1 (MQ=0)
                                                                          CAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGC                                        <  SRR10918232.3907714/151‑1 (MQ=0)
                                                                           AGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCG                                       >  SRR10918232.757337/1‑151 (MQ=0)
                                                                           AGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCG                                       <  SRR10918232.3954031/151‑1 (MQ=0)
                                                                            GCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGG                                      <  SRR10918232.4215526/151‑1 (MQ=0)
                                                                               CGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGG                                   <  SRR10918232.2141716/151‑1 (MQ=0)
                                                                                  CCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAG                                <  SRR10918232.2242395/151‑1 (MQ=0)
                                                                                         CGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCC                         <  SRR10918232.4803364/151‑1 (MQ=0)
                                                                                           GCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGG                       <  SRR10918232.4199022/151‑1 (MQ=0)
                                                                                               TTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCC                   >  SRR10918232.1241930/1‑151 (MQ=0)
                                                                                                 GCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTG                 >  SRR10918232.6500700/1‑151 (MQ=0)
                                                                                                  CCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGG                >  SRR10918232.2127340/1‑151 (MQ=0)
                                                                                                   CAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGT               <  SRR10918232.3263386/151‑1 (MQ=0)
                                                                                                    AGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTC              <  SRR10918232.3359623/151‑1 (MQ=0)
                                                                                                      TTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCA            <  SRR10918232.296896/151‑1 (MQ=0)
                                                                                                      TTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCA            >  SRR10918232.5005616/1‑151 (MQ=0)
                                                                                                       TCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCCCCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCAT           <  SRR10918232.4871449/151‑1 (MQ=0)
                                                                                                            TTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCATGAAGT      >  SRR10918232.2431573/1‑151 (MQ=0)
                                                                                                               CCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCATGAAGTGGC   <  SRR10918232.5936837/151‑1 (MQ=0)
                                                                                                                CCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCATGAAGTGGCG  <  SRR10918232.3732678/151‑1 (MQ=0)
                                                                                                                                                     |                                                                                                                 
GGTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGTTCACCGGGCGGGGGGAGTTTGTCCGGGCCGTGGTCCATGAAGTGGCG  >  CP009974/4536959‑4537221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑