Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 404 265.1 10478538 96.8% 10143224 150.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,209 A→G intergenic (+461/‑560) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,2090AG100.0% 68.9 / NA 44intergenic (+461/‑560)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTC  >  CP009974/4147068‑4147246
                                                                                                                                                    |                                     
ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                     <  1:4941841/151‑1 (MQ=11)
 gCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTg                                    >  2:2268442/1‑151 (MQ=11)
  cGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTgg                                   >  2:3176323/1‑151 (MQ=11)
   gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  <  1:2172156/151‑1 (MQ=11)
   gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  >  1:285785/1‑151 (MQ=11)
   gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                  >  2:1840035/1‑151 (MQ=11)
    aaaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcg                                 <  2:1145600/151‑1 (MQ=11)
     aaaGGTGAGTCAGCGTCGCCATCGCAAGTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  2:4071716/1‑151 (MQ=11)
     aaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  1:2603871/1‑151 (MQ=11)
     aaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                >  2:448572/1‑151 (MQ=11)
      aaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCa                               >  1:1261695/1‑151 (MQ=11)
        ggTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATa                             <  2:5058405/151‑1 (MQ=11)
         gTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAg                            >  2:4743152/1‑151 (MQ=11)
          tGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGt                           <  2:1161906/151‑1 (MQ=11)
          tGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGt                           <  1:4721095/151‑1 (MQ=11)
           gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                          <  2:40469/151‑1 (MQ=11)
           gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                          <  2:3092968/151‑1 (MQ=11)
             gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCCTAGTCAc                        >  2:404306/1‑151 (MQ=11)
             gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:5170870/151‑1 (MQ=11)
             gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:3801178/151‑1 (MQ=11)
             gTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAc                        <  2:2196851/151‑1 (MQ=11)
              tCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCAcc                       <  2:2190766/151‑1 (MQ=11)
                aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  1:3903814/1‑151 (MQ=11)
                aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  2:104930/1‑151 (MQ=11)
                aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtt                     >  1:581185/1‑151 (MQ=11)
                  cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                   >  1:4072577/1‑151 (MQ=11)
                  cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                   >  1:1333256/1‑151 (MQ=11)
                   gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCtttac                  <  2:1754817/151‑3 (MQ=11)
                   gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  <  2:1754818/151‑1 (MQ=11)
                   gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  <  1:873288/151‑1 (MQ=11)
                   gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTAGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                  >  1:3070718/1‑151 (MQ=11)
                    tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                 >  2:1249817/1‑151 (MQ=11)
                    tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                 >  2:4123263/1‑151 (MQ=11)
                     cGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGc                <  1:1021427/151‑1 (MQ=11)
                     cGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGc                >  1:7982/1‑151 (MQ=11)
                       ccATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGATTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGccc              <  2:2603737/151‑1 (MQ=11)
                        cATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCt             >  1:2919498/1‑151 (MQ=11)
                        cATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCt             >  1:2247284/1‑151 (MQ=11)
                          tCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa           <  2:189014/151‑1 (MQ=11)
                          tCGCAAATGGACTCTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa           <  1:5046223/151‑1 (MQ=11)
                              aaaTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggc       >  1:3363215/1‑151 (MQ=11)
                                aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggcgg     <  1:2832746/151‑1 (MQ=11)
                                 tGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCggg    <  2:7982/151‑1 (MQ=11)
                                   gACTGTTCACTGAGCCAAAACAGACGAGTTCACTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTAGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTc  <  1:447663/151‑1 (MQ=11)
                                                                                                                                                    |                                     
GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTC  >  CP009974/4147068‑4147246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A