Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 238,195 G→T 100% V532V (GTC→GTA)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273238,1950GT98.7% 807.7 / 1.5 233V532V (GTC→GTA) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (114/116);  total (115/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCA  >  CP009273/238048‑238343
                                                                                                                                                   |                                                                                                                                                    
    ttCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCa                                                                                                                                                <  1:3404439/150‑1 (MQ=255)
      cAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                              <  1:886110/150‑1 (MQ=255)
       aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTg                                                                                                                                             <  2:3753635/150‑1 (MQ=255)
       aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTg                                                                                                                                             <  2:3263268/150‑1 (MQ=255)
        ggTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGt                                                                                                                                            >  2:1705497/1‑150 (MQ=255)
           gCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttct                                                                                                                                         <  1:2843629/150‑1 (MQ=255)
            cTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttctt                                                                                                                                        <  2:873173/150‑1 (MQ=255)
             tGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttcttc                                                                                                                                       >  2:4487054/1‑150 (MQ=255)
             tGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttcttc                                                                                                                                       <  2:1949391/150‑1 (MQ=255)
              gATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcg                                                                                                                                      >  1:1113062/1‑150 (MQ=255)
                 agtagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgccg                                                                                                                                   <  2:1467089/150‑1 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                 <  1:4039867/150‑1 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                 >  2:3914404/1‑150 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                 >  2:3914270/1‑150 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                 <  1:491437/150‑1 (MQ=255)
                         ttAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCCTTTACGTCATTGTTCTTCGCCGCTTCCAtc                                                                                                                           <  1:3893154/150‑1 (MQ=255)
                          tAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtct                                                                                                                          >  1:1214870/1‑150 (MQ=255)
                            gTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATctct                                                                                                                        >  2:3840789/1‑150 (MQ=255)
                              ggCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTc                                                                                                                      >  2:2731005/1‑150 (MQ=255)
                                     ccGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACg                                                                                                               <  1:2481101/150‑1 (MQ=255)
                                        gTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTAc                                                                                                            <  2:654715/150‑1 (MQ=255)
                                          tGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACgg                                                                                                          >  2:3908512/1‑150 (MQ=255)
                                          tGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACgg                                                                                                          <  1:3987283/150‑1 (MQ=255)
                                           ggCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGa                                                                                                         >  1:1204462/1‑150 (MQ=255)
                                            gCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGAt                                                                                                        <  2:2089540/150‑1 (MQ=255)
                                             cGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATg                                                                                                       <  1:1776404/150‑1 (MQ=255)
                                             cGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATg                                                                                                       <  1:3938426/150‑1 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                     <  2:4542909/150‑1 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                     >  2:2986338/1‑150 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                     <  2:3995907/150‑1 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTAATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                     >  1:2327052/1‑150 (MQ=255)
                                                          aaaCCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCt                                                                                          <  1:2175815/150‑1 (MQ=255)
                                                          aaaCCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCt                                                                                          <  2:3292793/150‑1 (MQ=255)
                                                          aaaCCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCt                                                                                          >  2:3350057/1‑150 (MQ=255)
                                                           aaCCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTc                                                                                         <  1:2369486/150‑1 (MQ=255)
                                                             ccGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCt                                                                                       <  2:4240044/150‑1 (MQ=255)
                                                              cgcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCtt                                                                                      >  2:3703173/1‑150 (MQ=255)
                                                              cgcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCtt                                                                                      <  2:457902/150‑1 (MQ=255)
                                                                   gTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                                 <  2:1149387/150‑1 (MQ=255)
                                                                   gTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                                 >  2:2676838/1‑150 (MQ=255)
                                                                     cAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGaa                                                                               >  1:3183656/1‑150 (MQ=255)
                                                                     cAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGaa                                                                               >  1:3184091/1‑150 (MQ=255)
                                                                      aGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAg                                                                              >  2:1696803/1‑150 (MQ=255)
                                                                      aGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAg                                                                              <  1:818539/150‑1 (MQ=255)
                                                                           cagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcat                                                                         <  2:3502573/150‑1 (MQ=255)
                                                                           cagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcat                                                                         >  1:352340/1‑150 (MQ=255)
                                                                           cagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcat                                                                         <  2:3502601/150‑1 (MQ=255)
                                                                           cagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcat                                                                         >  2:1357839/1‑150 (MQ=255)
                                                                            agccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcatc                                                                        >  2:2384912/1‑150 (MQ=255)
                                                                             gccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGAtcatca                                                                       <  1:2612488/150‑1 (MQ=255)
                                                                             gccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGAtcatca                                                                       <  1:1404425/150‑1 (MQ=255)
                                                                              ccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATcatcat                                                                      <  2:944348/150‑1 (MQ=255)
                                                                              ccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATcatcat                                                                      <  2:2815658/150‑1 (MQ=255)
                                                                               cagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtg                                                                     <  1:3073931/150‑1 (MQ=255)
                                                                               cagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtg                                                                     <  2:2055016/150‑1 (MQ=255)
                                                                                agAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgc                                                                    >  2:3950725/1‑150 (MQ=255)
                                                                                agAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgc                                                                    >  2:2611351/1‑150 (MQ=255)
                                                                                 gAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgct                                                                   <  2:3855602/150‑1 (MQ=255)
                                                                                  aaGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgctg                                                                  <  2:2451648/150‑1 (MQ=255)
                                                                                     cTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCgg                                                               >  1:2832270/1‑150 (MQ=255)
                                                                                     cTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCgg                                                               >  1:653403/1‑150 (MQ=255)
                                                                                        cGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTc                                                            >  2:2284899/1‑150 (MQ=255)
                                                                                         gAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCa                                                           >  2:3583409/1‑150 (MQ=255)
                                                                                          aaCTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAg                                                          <  2:644751/150‑1 (MQ=255)
                                                                                           aCTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGa                                                         >  1:3774076/1‑150 (MQ=255)
                                                                                             tttGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAAt                                                       <  1:4318983/150‑1 (MQ=255)
                                                                                               tgttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGt                                                     <  1:3315741/150‑1 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                   <  1:4186024/150‑1 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                   <  1:3463667/150‑1 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                   <  1:4185966/150‑1 (MQ=255)
                                                                                                      aCCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcccc                                              <  2:3049886/150‑1 (MQ=255)
                                                                                                      aCCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcccc                                              <  2:3930887/150‑1 (MQ=255)
                                                                                                      aCCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcccc                                              <  2:3930832/150‑1 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                             >  2:2836678/1‑150 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                             <  1:1998678/150‑1 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                             >  1:2308132/1‑150 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCGTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                             <  1:3013259/150‑1 (MQ=255)
                                                                                                         gACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTc                                           >  1:2948308/1‑150 (MQ=255)
                                                                                                            gTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAc                                        >  1:1529766/1‑150 (MQ=255)
                                                                                                              gACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACgg                                      >  1:766973/1‑150 (MQ=255)
                                                                                                               aCCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGt                                     >  1:1453370/1‑150 (MQ=255)
                                                                                                               aCCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGt                                     >  1:4204608/1‑150 (MQ=255)
                                                                                                                 cGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCTAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGa                                   <  2:604357/150‑1 (MQ=255)
                                                                                                                 cGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGa                                   >  2:2888805/1‑150 (MQ=255)
                                                                                                                  gATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGAt                                  >  1:4348926/1‑150 (MQ=255)
                                                                                                                      tGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCa                              >  2:4167751/1‑150 (MQ=255)
                                                                                                                         ttGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATc                           <  1:2096476/150‑1 (MQ=255)
                                                                                                                             acaacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTg                       >  1:2758781/1‑150 (MQ=255)
                                                                                                                              caacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgc                      >  2:982461/1‑150 (MQ=255)
                                                                                                                               aacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgcg                     >  2:257978/1‑150 (MQ=255)
                                                                                                                                acaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgcgc                    >  2:1759593/1‑150 (MQ=255)
                                                                                                                                acaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTAAGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgcgc                    >  2:1147956/1‑150 (MQ=255)
                                                                                                                                 caGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGNCCCTTCAACGGTGATGGCAATCGGTGCGcc                   <  1:4281416/150‑1 (MQ=255)
                                                                                                                                 caGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGcc                   <  2:1162689/150‑1 (MQ=255)
                                                                                                                                   gTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCt                 >  2:3728156/1‑150 (MQ=255)
                                                                                                                                   gTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCt                 <  1:65161/150‑1 (MQ=255)
                                                                                                                                   gTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCACTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCt                 <  1:1089268/150‑1 (MQ=255)
                                                                                                                                      tATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGt              >  2:3677705/1‑150 (MQ=255)
                                                                                                                                         cGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAg           >  2:1433192/1‑150 (MQ=255)
                                                                                                                                               cgTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAcgcg     >  1:3845063/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCA  >  CP009273/238048‑238343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238046‑238344
                                                                                                                                                     |                                                                                                                                                     
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       <  SRR19973072.4006753/150‑1 (MQ=60)
 GGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTA                                                                                                                                                      <  SRR19973072.3870520/150‑1 (MQ=60)
 GGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTA                                                                                                                                                      >  SRR19973072.3028297/1‑150 (MQ=60)
 GGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTA                                                                                                                                                      >  SRR19973072.2795867/1‑150 (MQ=60)
      TTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCA                                                                                                                                                 <  SRR19973072.3411631/150‑1 (MQ=60)
        CAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATT                                                                                                                                               <  SRR19973072.110438/150‑1 (MQ=60)
        CAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATT                                                                                                                                               <  SRR19973072.888013/150‑1 (MQ=60)
            TGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTC                                                                                                                                           >  SRR19973072.3067842/1‑150 (MQ=60)
             GCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCT                                                                                                                                          <  SRR19973072.2849712/150‑1 (MQ=60)
             GCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCT                                                                                                                                          <  SRR19973072.2849768/150‑1 (MQ=60)
              CTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTT                                                                                                                                         <  SRR19973072.4421009/150‑1 (MQ=60)
                GATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCG                                                                                                                                       >  SRR19973072.1115412/1‑150 (MQ=60)
                  TAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCC                                                                                                                                     >  SRR19973072.1147215/1‑150 (MQ=60)
                     TAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCT                                                                                                                                  <  SRR19973072.4048317/150‑1 (MQ=60)
                     TAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCT                                                                                                                                  <  SRR19973072.492597/150‑1 (MQ=60)
                     TAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCT                                                                                                                                  <  SRR19973072.4048345/150‑1 (MQ=60)
                     TAGCGTTTAGTGGAATCGCCGGTTGGCGTGCTGCTTGTTAAACCGCGCGTAAGGCCCATCCAGAATCTGCTAACTTTTTTGCTACCGACTTTACCGATATGTTTGAAAAACAGTTTATCGTACGCGTTTACTTCATTGTTCTTggcagat                                                                                                                                  <  SRR19973072.498576/150‑8 (MQ=60)
                      AGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTT                                                                                                                                 >  SRR19973072.3082808/1‑150 (MQ=60)
                         GTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCA                                                                                                                              <  SRR19973072.791262/150‑1 (MQ=60)
                           TTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCCTTTACGTCATTGTTCTTCGCCGCTTCCATC                                                                                                                            <  SRR19973072.3901255/150‑1 (MQ=60)
                           TTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCCTTTACGTCATTGTTCTTCGCCGCTTCCATC                                                                                                                            <  SRR19973072.3901241/150‑1 (MQ=60)
                           TTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCCTTTACGTCATTGTTCTTCGCCGCTTCCATC                                                                                                                            <  SRR19973072.3901303/150‑1 (MQ=60)
                           TTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCCTTTACGTCATTGTTCTTCGCCGCTTCCATC                                                                                                                            <  SRR19973072.3901261/150‑1 (MQ=60)
                            TAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCT                                                                                                                           >  SRR19973072.1217467/1‑150 (MQ=60)
                            TAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCT                                                                                                                           >  SRR19973072.1217514/1‑150 (MQ=60)
                             AGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTC                                                                                                                          >  SRR19973072.2974526/1‑150 (MQ=60)
                               TGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTT                                                                                                                        >  SRR19973072.2166445/1‑150 (MQ=60)
                                       CCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACG                                                                                                                <  SRR19973072.2486484/150‑1 (MQ=60)
                                        CGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGT                                                                                                               >  SRR19973072.234190/1‑150 (MQ=60)
                                        CGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGT                                                                                                               >  SRR19973072.234198/1‑150 (MQ=60)
                                          GTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTAC                                                                                                             <  SRR19973072.3403863/150‑1 (MQ=60)
                                            TGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGG                                                                                                           <  SRR19973072.3995630/150‑1 (MQ=60)
                                             GGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGA                                                                                                          >  SRR19973072.1207032/1‑150 (MQ=60)
                                               CGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATG                                                                                                        <  SRR19973072.1780292/150‑1 (MQ=60)
                                               CGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATG                                                                                                        <  SRR19973072.3946782/150‑1 (MQ=60)
                                               CGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATG                                                                                                        <  SRR19973072.3946650/150‑1 (MQ=60)
                                                 TGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTAATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGC                                                                                                      >  SRR19973072.2332037/1‑150 (MQ=60)
                                                    TGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAAC                                                                                                   >  SRR19973072.425393/1‑150 (MQ=60)
                                                       TGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAA                                                                                                >  SRR19973072.3608493/1‑150 (MQ=60)
                                                        GGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAAT                                                                                               <  SRR19973072.2043853/150‑1 (MQ=60)
                                                            AAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCT                                                                                           <  SRR19973072.2180554/150‑1 (MQ=60)
                                                             AACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTC                                                                                          <  SRR19973072.2374597/150‑1 (MQ=60)
                                                                CGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTT                                                                                       >  SRR19973072.1292294/1‑150 (MQ=60)
                                                                CGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTT                                                                                       <  SRR19973072.2843711/150‑1 (MQ=60)
                                                                 GCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTG                                                                                      >  SRR19973072.1208695/1‑150 (MQ=60)
                                                                     GTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCG                                                                                  <  SRR19973072.4324481/150‑1 (MQ=60)
                                                                     GTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCG                                                                                  <  SRR19973072.4285320/150‑1 (MQ=60)
                                                                       CAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAA                                                                                >  SRR19973072.3190805/1‑150 (MQ=60)
                                                                       CAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAA                                                                                >  SRR19973072.3190375/1‑150 (MQ=60)
                                                                       CAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAA                                                                                >  SRR19973072.3190369/1‑150 (MQ=60)
                                                                        AGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAG                                                                               <  SRR19973072.820297/150‑1 (MQ=60)
                                                                        AGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAG                                                                               <  SRR19973072.4059017/150‑1 (MQ=60)
                                                                             CAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCAT                                                                          >  SRR19973072.353123/1‑150 (MQ=60)
                                                                               GCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCA                                                                        <  SRR19973072.2618117/150‑1 (MQ=60)
                                                                               GCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCA                                                                        <  SRR19973072.2618125/150‑1 (MQ=60)
                                                                               GCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCA                                                                        <  SRR19973072.1407483/150‑1 (MQ=60)
                                                                                 CAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATG                                                                      <  SRR19973072.3080423/150‑1 (MQ=60)
                                                                                 CAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATG                                                                      <  SRR19973072.2837847/150‑1 (MQ=60)
                                                                                       CTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGG                                                                <  SRR19973072.1650960/150‑1 (MQ=60)
                                                                                       CTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGG                                                                >  SRR19973072.654883/1‑150 (MQ=60)
                                                                                       CTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGG                                                                >  SRR19973072.2838335/1‑150 (MQ=60)
                                                                                          CGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTC                                                             >  SRR19973072.2862859/1‑150 (MQ=60)
                                                                                             ACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGA                                                          >  SRR19973072.3391038/1‑150 (MQ=60)
                                                                                             ACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGA                                                          >  SRR19973072.3781990/1‑150 (MQ=60)
                                                                                               TTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAAT                                                        <  SRR19973072.4328017/150‑1 (MQ=60)
                                                                                                 TGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGT                                                      <  SRR19973072.3322782/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.3470931/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.4194734/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.4194726/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.4194784/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.4195161/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973072.3470918/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              <  SRR19973072.2002984/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              >  SRR19973072.2313059/1‑150 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCGTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              <  SRR19973072.3019662/150‑1 (MQ=60)
                                                                                                           GACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTC                                            >  SRR19973072.2954610/1‑150 (MQ=60)
                                                                                                              GTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAC                                         <  SRR19973072.346617/150‑1 (MQ=60)
                                                                                                              GTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAC                                         >  SRR19973072.1533006/1‑150 (MQ=60)
                                                                                                              GTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAC                                         <  SRR19973072.2558326/150‑1 (MQ=60)
                                                                                                                GACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGG                                       >  SRR19973072.768594/1‑150 (MQ=60)
                                                                                                                GACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGG                                       <  SRR19973072.2177641/150‑1 (MQ=60)
                                                                                                                 ACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGT                                      >  SRR19973072.4213395/1‑150 (MQ=60)
                                                                                                                 ACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGT                                      >  SRR19973072.1456495/1‑150 (MQ=60)
                                                                                                                 ACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGT                                      >  SRR19973072.832079/1‑150 (MQ=60)
                                                                                                                  CCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTG                                     <  SRR19973072.1948398/150‑1 (MQ=60)
                                                                                                                  CCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTG                                     <  SRR19973072.3710978/150‑1 (MQ=60)
                                                                                                                    GATATGTTTTAACAACAGTTTATCTAACGCGTTTACTTCAAAGTTCTACGCCGCTTCCAACTCATCCAGCACGTACGGATGGCACAGAAACGCTCCGTGTCCGAAGATCATCAAGCTGCGGGTCATAATGTTAGCCCCTTCAAAGGTGAT                                   >  SRR19973072.4357984/1‑150 (MQ=60)
                                                                                                                    GATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGAT                                   >  SRR19973072.4358042/1‑150 (MQ=60)
                                                                                                                     ATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATG                                  >  SRR19973072.1557231/1‑150 (MQ=60)
                                                                                                                        TGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCA                               >  SRR19973072.2329978/1‑150 (MQ=60)
                                                                                                                        TGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCA                               >  SRR19973072.14506/1‑150 (MQ=60)
                                                                                                                           TTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATC                            <  SRR19973072.2101049/150‑1 (MQ=60)
                                                                                                                              AACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGT                         >  SRR19973072.382267/1‑150 (MQ=60)
                                                                                                                               ACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTG                        >  SRR19973072.3198610/1‑150 (MQ=60)
                                                                                                                               ACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTG                        >  SRR19973072.2764681/1‑150 (MQ=60)
                                                                                                                                CAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGC                       >  SRR19973072.1521460/1‑150 (MQ=60)
                                                                                                                                   CAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGNCCCTTCAACGGTGATGGCAATCGGTGCGCC                    <  SRR19973072.4290325/150‑1 (MQ=60)
                                                                                                                                     GTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCT                  <  SRR19973072.65396/150‑1 (MQ=60)
                                                                                                                                     GTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCT                  <  SRR19973072.65335/150‑1 (MQ=60)
                                                                                                                                     GTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCACTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCT                  <  SRR19973072.1091572/150‑1 (MQ=60)
                                                                                                                                        TATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGT               >  SRR19973072.3133160/1‑150 (MQ=60)
                                                                                                                                        TATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGT               >  SRR19973072.2262350/1‑150 (MQ=60)
                                                                                                                                         ATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTA              >  SRR19973072.4103214/1‑150 (MQ=60)
                                                                                                                                           CGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAG            >  SRR19973072.2410441/1‑150 (MQ=60)
                                                                                                                                              ACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAC         <  SRR19973072.2679871/150‑1 (MQ=60)
                                                                                                                                                 CGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCG      >  SRR19973072.3853084/1‑150 (MQ=60)
                                                                                                                                                  GTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGC     >  SRR19973072.729217/1‑150 (MQ=60)
                                                                                                                                                  GTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGC     >  SRR19973072.729221/1‑150 (MQ=60)
                                                                                                                                                     TACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  <  SRR19973072.2727868/150‑1 (MQ=60)
                                                                                                                                                     TACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  <  SRR19973072.844160/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238046‑238344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑