Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 238,195 G→T 83.3% V532V (GTC→GTA)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273238,1950GT83.3% 603.4 / 127.6 246V532V (GTC→GTA) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (18/23);  new base T (98/107);  total (116/130)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 248 total aligned reads displayed.

GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238048‑238344
                                                                                                                                                   |                                                                                                                                                     
gCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTAc                                                                                                                                                     <  1:3469412/150‑1 (MQ=255)
gCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGAc                                                                                                                                                     <  1:3158698/150‑1 (MQ=255)
  ggTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGt                                                                                                                                                   >  2:1059063/1‑150 (MQ=255)
  ggTTCAGGTGCTGATAGTACCGTTTAGTGGCATCGCCGGTTTGCGTGCTGCAGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAAATTTGTTGCTACCGACCTCAACGATATGTTTGAAAAACAGTTTATAGAACGCGTATACGt                                                                                                                                                   >  2:3100220/1‑150 (MQ=255)
      cAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                               <  2:3240551/150‑1 (MQ=255)
      cAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                               <  1:1520707/150‑1 (MQ=255)
          tGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGttc                                                                                                                                           <  2:4730984/150‑1 (MQ=255)
               aTAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgc                                                                                                                                      >  1:4194324/1‑150 (MQ=255)
               aTAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgc                                                                                                                                      >  1:3958812/1‑150 (MQ=255)
                tagtagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgcc                                                                                                                                     <  2:1393057/150‑1 (MQ=255)
                  gtagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCGGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACGACAGGTTATCGAACGCGGTTACGTCATGGTTCTTcgccgc                                                                                                                                   >  2:414304/1‑150 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                  >  1:372989/1‑150 (MQ=255)
                   tagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCACAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCt                                                                                                                                  <  2:1506528/150‑1 (MQ=255)
                    agCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCtt                                                                                                                                 <  1:1151955/150‑1 (MQ=255)
                    agCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCtt                                                                                                                                 <  2:1230645/150‑1 (MQ=255)
                           aGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtctc                                                                                                                          <  1:2659943/150‑1 (MQ=255)
                             tGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCtt                                                                                                                        <  1:4579096/150‑1 (MQ=255)
                              ggCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTc                                                                                                                       >  1:2393736/1‑150 (MQ=255)
                                cATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCa                                                                                                                     <  2:584407/150‑1 (MQ=255)
                                 aTCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGTACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAg                                                                                                                    >  2:3469648/1‑150 (MQ=255)
                                  tCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTCTCGAACGCGTTTACGTCAGTGTTCTTCGCCGCTTCCATCTCTTCCAGc                                                                                                                   >  2:2511483/1‑150 (MQ=255)
                                  tCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGc                                                                                                                   >  1:2790070/1‑150 (MQ=255)
                                  tCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGc                                                                                                                   <  1:4327467/150‑1 (MQ=255)
                                  tCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGc                                                                                                                   >  1:4760843/1‑150 (MQ=255)
                                    gCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCAc                                                                                                                 <  2:3025839/150‑1 (MQ=255)
                                    gCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCAc                                                                                                                 <  2:808716/150‑1 (MQ=255)
                                      cGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTATTCGCCGCTTCCATCTCTTCCAGCACGt                                                                                                               >  2:5111739/1‑150 (MQ=255)
                                       ggTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTa                                                                                                              >  2:4851835/1‑150 (MQ=255)
                                           ggCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGa                                                                                                          <  2:1493379/150‑1 (MQ=255)
                                           ggCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGa                                                                                                          <  2:3332658/150‑1 (MQ=255)
                                            gCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGAt                                                                                                         <  1:2191891/150‑1 (MQ=255)
                                             cGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATg                                                                                                        <  1:3909227/150‑1 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                      <  2:3603304/150‑1 (MQ=255)
                                               tgctgcTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGc                                                                                                      <  2:1566437/150‑1 (MQ=255)
                                                 ctgctgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCaa                                                                                                    >  2:134418/1‑150 (MQ=255)
                                                 ctgctgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCaa                                                                                                    >  2:2749727/1‑150 (MQ=255)
                                                 ctgctgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCaa                                                                                                    >  2:1785624/1‑150 (MQ=255)
                                                     tgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGaa                                                                                                <  2:2086546/150‑1 (MQ=255)
                                                      ggTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAAt                                                                                               <  1:370476/150‑1 (MQ=255)
                                                       gTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATc                                                                                              <  2:3823712/150‑1 (MQ=255)
                                                       gTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATc                                                                                              <  2:445967/150‑1 (MQ=255)
                                                         tAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGc                                                                                            <  1:2145004/150‑1 (MQ=255)
                                                          aaaCCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCt                                                                                           >  1:4966290/1‑150 (MQ=255)
                                                             ccGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCt                                                                                        >  1:5188706/1‑150 (MQ=255)
                                                              cgcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCtt                                                                                       <  1:2441919/150‑1 (MQ=255)
                                                                   gTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                                  >  1:2836078/1‑150 (MQ=255)
                                                                   gTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                                  <  2:5126133/150‑1 (MQ=255)
                                                                     cAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGaa                                                                                >  2:3654579/1‑150 (MQ=255)
                                                                      aGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAg                                                                               >  1:2004811/1‑150 (MQ=255)
                                                                        gCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGat                                                                             <  2:1548537/150‑1 (MQ=255)
                                                                        gCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGat                                                                             <  1:79450/150‑1 (MQ=255)
                                                                          ccagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatca                                                                           >  1:2846539/1‑150 (MQ=255)
                                                                          ccagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatca                                                                           >  1:779632/1‑150 (MQ=255)
                                                                               cagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtg                                                                      <  2:1669259/150‑1 (MQ=255)
                                                                                 gAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgct                                                                    <  1:1937894/150‑1 (MQ=255)
                                                                                      tGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCggg                                                               >  1:1050638/1‑150 (MQ=255)
                                                                                        cGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTc                                                             <  2:4532332/150‑1 (MQ=255)
                                                                                        cGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTc                                                             <  1:683010/150‑1 (MQ=255)
                                                                                         gAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCTAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTcg                                                            <  1:2088418/150‑2 (MQ=255)
                                                                                          aaCTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAg                                                           >  2:2109478/1‑150 (MQ=255)
                                                                                          aaCTTTGTTGCTACCGACGTGACCGATATGTTTGAACAAACGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCAGCTTACATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGGCAg                                                           >  2:3834177/1‑150 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                    <  1:3219905/150‑1 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                    <  1:614204/150‑1 (MQ=255)
                                                                                                 ttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTa                                                    <  1:3354800/150‑1 (MQ=255)
                                                                                                    cTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcc                                                 <  2:3412394/150‑1 (MQ=255)
                                                                                                      aCCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcccc                                               <  1:3053530/150‑1 (MQ=255)
                                                                                                      aCCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGcccc                                               <  1:443230/150‑1 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                              <  1:3393573/150‑1 (MQ=255)
                                                                                                       ccGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                              <  1:1622954/150‑1 (MQ=255)
                                                                                                          aCGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCa                                           <  2:1620080/150‑1 (MQ=255)
                                                                                                          aCGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCa                                           <  1:826983/150‑1 (MQ=255)
                                                                                                             tGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACg                                        <  1:1429417/150‑1 (MQ=255)
                                                                                                              gACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACgg                                       >  1:3584397/1‑150 (MQ=255)
                                                                                                                ccGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCGTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTg                                     >  2:4112447/1‑150 (MQ=255)
                                                                                                                 cGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGa                                    >  2:3944370/1‑150 (MQ=255)
                                                                                                                  gATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGAt                                   >  1:1777886/1‑150 (MQ=255)
                                                                                                                   atatGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATg                                  >  2:4709758/1‑150 (MQ=255)
                                                                                                                      tGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCa                               <  1:4922893/150‑1 (MQ=255)
                                                                                                                       gTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCaa                              >  1:4678857/1‑150 (MQ=255)
                                                                                                                       gTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCaa                              <  2:3542098/150‑1 (MQ=255)
                                                                                                                        tttGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAAt                             >  2:1049066/1‑150 (MQ=255)
                                                                                                                         ttGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATc                            <  1:3646951/150‑1 (MQ=255)
                                                                                                                         ttGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATc                            <  1:2592139/150‑1 (MQ=255)
                                                                                                                           gAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCgg                          <  1:1869356/150‑1 (MQ=255)
                                                                                                                           gAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCgg                          <  1:955151/150‑1 (MQ=255)
                                                                                                                            aacaacAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGt                         >  1:4544712/1‑150 (MQ=255)
                                                                                                                               aacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgcg                      >  2:2984583/1‑150 (MQ=255)
                                                                                                                                acaGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgcgc                     >  2:5100576/1‑150 (MQ=255)
                                                                                                                                 caGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGcc                    <  2:459147/150‑1 (MQ=255)
                                                                                                                                  aGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGccc                   <  1:4131151/150‑1 (MQ=255)
                                                                                                                                     ttATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTgg                <  2:1888288/150‑1 (MQ=255)
                                                                                                                                       aTCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTa              >  1:1237937/1‑150 (MQ=255)
                                                                                                                                       aTCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTa              >  2:4300319/1‑150 (MQ=255)
                                                                                                                                        tCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTaa             <  2:3986311/150‑1 (MQ=255)
                                                                                                                                         cGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAg            >  2:2127236/1‑150 (MQ=255)
                                                                                                                                           aaCGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCa          <  2:82948/150‑1 (MQ=255)
                                                                                                                                             cgcgTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAcg        >  2:2626041/1‑150 (MQ=255)
                                                                                                                                              gcgTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAcgc       >  2:11084/1‑150 (MQ=255)
                                                                                                                                               cgTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAcgcg      >  2:703593/1‑150 (MQ=255)
                                                                                                                                                  tGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCa   >  2:2223701/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238048‑238344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238046‑238344
                                                                                                                                                     |                                                                                                                                                     
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       >  SRR19973083.3040398/1‑150 (MQ=60)
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       >  SRR19973083.3040217/1‑150 (MQ=60)
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       >  SRR19973083.3851690/1‑150 (MQ=60)
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       >  SRR19973083.3851212/1‑150 (MQ=60)
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTT                                                                                                                                                       >  SRR19973083.3039964/1‑150 (MQ=60)
  GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTAC                                                                                                                                                     <  SRR19973083.3485276/150‑1 (MQ=60)
  GCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGAC                                                                                                                                                     <  SRR19973083.3173767/150‑1 (MQ=60)
   CGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACG                                                                                                                                                    <  SRR19973083.91450/150‑1 (MQ=60)
   CGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACG                                                                                                                                                    <  SRR19973083.91213/150‑1 (MQ=60)
   CGGTTCAGGTGCTGATAGGAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACG                                                                                                                                                    <  SRR19973083.825545/150‑1 (MQ=60)
       TCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAT                                                                                                                                                >  SRR19973083.405727/1‑150 (MQ=60)
        CAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATT                                                                                                                                               <  SRR19973083.1528355/150‑1 (MQ=60)
         AGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTG                                                                                                                                              >  SRR19973083.14445/1‑150 (MQ=60)
          GGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGT                                                                                                                                             <  SRR19973083.3492194/150‑1 (MQ=60)
           GTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTT                                                                                                                                            >  SRR19973083.1114241/1‑150 (MQ=60)
            TGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTC                                                                                                                                           <  SRR19973083.4827/150‑1 (MQ=60)
            TGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTC                                                                                                                                           <  SRR19973083.4609717/150‑1 (MQ=60)
               TGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTC                                                                                                                                        >  SRR19973083.635490/1‑150 (MQ=60)
                 ATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGC                                                                                                                                      >  SRR19973083.3976444/1‑150 (MQ=60)
                 ATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGC                                                                                                                                      >  SRR19973083.4212769/1‑150 (MQ=60)
                     TAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCT                                                                                                                                  >  SRR19973083.374732/1‑150 (MQ=60)
                      AGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTT                                                                                                                                 <  SRR19973083.1157696/150‑1 (MQ=60)
                             AGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTC                                                                                                                          <  SRR19973083.2673291/150‑1 (MQ=60)
                              GTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCT                                                                                                                         <  SRR19973083.591283/150‑1 (MQ=60)
                               TGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTT                                                                                                                        <  SRR19973083.4598895/150‑1 (MQ=60)
                                GGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTC                                                                                                                       >  SRR19973083.4024/1‑150 (MQ=60)
                                GGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTC                                                                                                                       >  SRR19973083.2108721/1‑150 (MQ=60)
                                GGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTC                                                                                                                       >  SRR19973083.2406044/1‑150 (MQ=60)
                                  CATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCA                                                                                                                     >  SRR19973083.4211251/1‑150 (MQ=60)
                                   ATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAG                                                                                                                    >  SRR19973083.2808535/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.792368/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   <  SRR19973083.4346245/150‑1 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.1606432/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.792527/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.2803936/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.4781133/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.4781114/1‑150 (MQ=60)
                                    TCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGC                                                                                                                   >  SRR19973083.4781184/1‑150 (MQ=60)
                                        CGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGT                                                                                                               >  SRR19973083.504591/1‑150 (MQ=60)
                                         GGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTA                                                                                                              >  SRR19973083.3282391/1‑150 (MQ=60)
                                         GGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTA                                                                                                              >  SRR19973083.3282386/1‑150 (MQ=60)
                                           TTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACG                                                                                                            >  SRR19973083.1897862/1‑150 (MQ=60)
                                              GCGTGCTGCTTGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATTTTTGAACAACAGTTTATCGAACGAGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTACAGCACGTACGGAT                                                                                                         <  SRR19973083.2202870/150‑1 (MQ=60)
                                              GCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGAT                                                                                                         <  SRR19973083.2202878/150‑1 (MQ=60)
                                               CGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATG                                                                                                        <  SRR19973083.3926624/150‑1 (MQ=60)
                                                        GGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAAT                                                                                               <  SRR19973083.3448965/150‑1 (MQ=60)
                                                        GGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAAT                                                                                               <  SRR19973083.372211/150‑1 (MQ=60)
                                                         GTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATC                                                                                              <  SRR19973083.1860062/150‑1 (MQ=60)
                                                           TAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGC                                                                                            <  SRR19973083.2155797/150‑1 (MQ=60)
                                                            AAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCT                                                                                           >  SRR19973083.4987622/1‑150 (MQ=60)
                                                               CCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCT                                                                                        <  SRR19973083.3713933/150‑1 (MQ=60)
                                                               CCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCT                                                                                        <  SRR19973083.3713034/150‑1 (MQ=60)
                                                               CCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCT                                                                                        >  SRR19973083.5210679/1‑150 (MQ=60)
                                                                CGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTT                                                                                       <  SRR19973083.2454440/150‑1 (MQ=60)
                                                                     GTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCG                                                                                  >  SRR19973083.2850106/1‑150 (MQ=60)
                                                                     GTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCG                                                                                  >  SRR19973083.2850103/1‑150 (MQ=60)
                                                                       CAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAA                                                                                <  SRR19973083.2738227/150‑1 (MQ=60)
                                                                       CAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAA                                                                                <  SRR19973083.2738305/150‑1 (MQ=60)
                                                                        AGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAG                                                                               >  SRR19973083.2015046/1‑150 (MQ=60)
                                                                          GCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGNT                                                                             <  SRR19973083.4560703/150‑1 (MQ=60)
                                                                          GCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGAT                                                                             <  SRR19973083.80018/150‑1 (MQ=60)
                                                                            CCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCA                                                                           >  SRR19973083.783420/1‑150 (MQ=60)
                                                                            CCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCA                                                                           >  SRR19973083.2860599/1‑150 (MQ=60)
                                                                             CAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCAT                                                                          <  SRR19973083.1365880/150‑1 (MQ=60)
                                                                                 CAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATG                                                                      <  SRR19973083.5074443/150‑1 (MQ=60)
                                                                                   GAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACTGATGTCAACGAATCGCTCCTTGTCCTAAGATCATCATGCT                                                                    <  SRR19973083.1948637/150‑1 (MQ=60)
                                                                                   GAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCT                                                                    <  SRR19973083.1947780/150‑1 (MQ=60)
                                                                                   GAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCT                                                                    <  SRR19973083.3126160/150‑1 (MQ=60)
                                                                                   GAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCT                                                                    <  SRR19973083.1947975/150‑1 (MQ=60)
                                                                                       CTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGG                                                                <  SRR19973083.186799/150‑1 (MQ=60)
                                                                                       CTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGG                                                                >  SRR19973083.3913749/1‑150 (MQ=60)
                                                                                        TGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGG                                                               >  SRR19973083.1055930/1‑150 (MQ=60)
                                                                                          CGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCCACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTC                                                             >  SRR19973083.3968641/1‑150 (MQ=60)
                                                                                          CGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTC                                                             >  SRR19973083.3212408/1‑150 (MQ=60)
                                                                                          CGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTC                                                             <  SRR19973083.686424/150‑1 (MQ=60)
                                                                                          CGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTC                                                             <  SRR19973083.685628/150‑1 (MQ=60)
                                                                                           GAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCTAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCG                                                            <  SRR19973083.2099019/150‑1 (MQ=60)
                                                                                           GAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCTAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCA                                                            <  SRR19973083.2099003/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973083.617286/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973083.3606102/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973083.3370424/150‑1 (MQ=60)
                                                                                                   TTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTA                                                    <  SRR19973083.3235160/150‑1 (MQ=60)
                                                                                                     GCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGACTGTTAGC                                                  >  SRR19973083.2545077/1‑150 (MQ=60)
                                                                                                        ACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCC                                               <  SRR19973083.445211/150‑1 (MQ=60)
                                                                                                        ACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCC                                               <  SRR19973083.3068294/150‑1 (MQ=60)
                                                                                                        ACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCC                                               <  SRR19973083.3068162/150‑1 (MQ=60)
                                                                                                        ACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCC                                               <  SRR19973083.4327482/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              <  SRR19973083.3409277/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              <  SRR19973083.1631057/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              <  SRR19973083.3409164/150‑1 (MQ=60)
                                                                                                         CCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCT                                              >  SRR19973083.2424680/1‑150 (MQ=60)
                                                                                                            ACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCA                                           <  SRR19973083.830908/150‑1 (MQ=60)
                                                                                                              GTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAC                                         >  SRR19973083.3724315/1‑150 (MQ=60)
                                                                                                               TGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACG                                        >  SRR19973083.2393386/1‑150 (MQ=60)
                                                                                                               TGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACG                                        <  SRR19973083.1436582/150‑1 (MQ=60)
                                                                                                                GACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGG                                       >  SRR19973083.3600820/1‑150 (MQ=60)
                                                                                                                  CCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTG                                     <  SRR19973083.2877055/150‑1 (MQ=60)
                                                                                                                  CCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTG                                     <  SRR19973083.250485/150‑1 (MQ=60)
                                                                                                                   CGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGA                                    >  SRR19973083.2644499/1‑150 (MQ=60)
                                                                                                                    GATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGAT                                   >  SRR19973083.1786739/1‑150 (MQ=60)
                                                                                                                     ATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATG                                  <  SRR19973083.1647066/150‑1 (MQ=60)
                                                                                                                        TGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCA                               <  SRR19973083.4589878/150‑1 (MQ=60)
                                                                                                                        TGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCA                               <  SRR19973083.4944079/150‑1 (MQ=60)
                                                                                                                        TGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCA                               <  SRR19973083.4944055/150‑1 (MQ=60)
                                                                                                                         GTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAA                              >  SRR19973083.4698865/1‑150 (MQ=60)
                                                                                                                         GTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAA                              >  SRR19973083.4698961/1‑150 (MQ=60)
                                                                                                                         CTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAA                              <  SRR19973083.3448398/150‑1 (MQ=60)
                                                                                                                           TTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATC                            <  SRR19973083.3663636/150‑1 (MQ=60)
                                                                                                                           TTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATC                            <  SRR19973083.2605194/150‑1 (MQ=60)
                                                                                                                           TTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATC                            <  SRR19973083.4519344/150‑1 (MQ=60)
                                                                                                                             GAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGG                          <  SRR19973083.959862/150‑1 (MQ=60)
                                                                                                                             GAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGG                          <  SRR19973083.1878808/150‑1 (MQ=60)
                                                                                                                              AACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGT                         >  SRR19973083.4564415/1‑150 (MQ=60)
                                                                                                                                CAACAGTTTATCGAACGCGTTTACGTCATTGTTCGTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGC                       <  SRR19973083.3816813/150‑1 (MQ=60)
                                                                                                                                 AACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCG                      >  SRR19973083.4436017/1‑150 (MQ=60)
                                                                                                                                    AGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCC                   <  SRR19973083.4149418/150‑1 (MQ=60)
                                                                                                                                     GTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCT                  <  SRR19973083.2145904/150‑1 (MQ=60)
                                                                                                                                         ATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTA              >  SRR19973083.1243810/1‑150 (MQ=60)
                                                                                                                                         ATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTA              <  SRR19973083.1561989/150‑1 (MQ=60)
                                                                                                                                         ATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTA              >  SRR19973083.1243994/1‑150 (MQ=60)
                                                                                                                                            GAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGC           >  SRR19973083.4288058/1‑150 (MQ=60)
                                                                                                                                             AACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCA          <  SRR19973083.1368676/150‑1 (MQ=60)
                                                                                                                                              ACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAC         <  SRR19973083.707652/150‑1 (MQ=60)
                                                                                                                                               CGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACG        <  SRR19973083.994227/150‑1 (MQ=60)
                                                                                                                                                    TTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCA   >  SRR19973083.4867045/1‑150 (MQ=60)
                                                                                                                                                    TTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCA   <  SRR19973083.3372865/150‑1 (MQ=60)
                                                                                                                                                    TTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCA   <  SRR19973083.3372903/150‑1 (MQ=60)
                                                                                                                                                     GACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  <  SRR19973083.233200/150‑1 (MQ=60)
                                                                                                                                                     GACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  <  SRR19973083.2859019/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAG  >  CP009273/238046‑238344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑