Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 451,853 C→A 5.9% T422N (ACC→AAC)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273451,8530CA 5.9% 1091.4 / 26.4 282T422N (ACC→AAC) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (165/100);  new base A (10/7);  total (175/107)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGC  >  CP009273/451704‑452002
                                                                                                                                                     |                                                                                                                                                     
agagATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAc                                                                                                                                                       >  2:4631259/1‑150 (MQ=255)
  agaTGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCa                                                                                                                                                     >  1:3273884/1‑150 (MQ=255)
      ggCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACttt                                                                                                                                                 >  1:1376172/1‑150 (MQ=255)
      ggCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACttt                                                                                                                                                 >  2:2437812/1‑150 (MQ=255)
        cTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTCCTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCa                                                                                                                                               >  2:3870920/1‑150 (MQ=255)
        cTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAAAGTAGCTCTGGAAGAACAGGCACTTGAAGCAGTACTGGCGAAAGCGAAAGTGACTGACAAAGAAACCACTTACa                                                                                                                                               >  2:1739125/1‑150 (MQ=255)
         ttCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCaa                                                                                                                                              <  1:1016317/150‑1 (MQ=255)
          tCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAAc                                                                                                                                             >  2:4501709/1‑150 (MQ=255)
           cTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACg                                                                                                                                            >  1:3546766/1‑150 (MQ=255)
            tGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGa                                                                                                                                           >  1:1010369/1‑150 (MQ=255)
            tGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGa                                                                                                                                           <  2:1847544/150‑1 (MQ=255)
               gTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCt                                                                                                                                        >  2:1240040/1‑150 (MQ=255)
                     aGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGaa                                                                                                                                  >  1:2395120/1‑150 (MQ=255)
                      gATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGTACAAAATGCGCAATGTTGCTCTGGAAGAACAGGGTGTTGAAGCTGTACTGGCGAAAGCGATAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAAc                                                                                                                                 >  2:306147/1‑150 (MQ=255)
                       aTCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAAcc                                                                                                                                >  2:4291895/1‑150 (MQ=255)
                       aTCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAAcc                                                                                                                                >  1:280284/1‑150 (MQ=255)
                          cGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACcagc                                                                                                                             >  1:197431/1‑150 (MQ=255)
                           gAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACcagca                                                                                                                            <  1:2602795/150‑1 (MQ=255)
                            aaaGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACcagcag                                                                                                                           >  1:3386398/1‑150 (MQ=255)
                              agaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGc                                                                                                                         >  2:4096564/1‑150 (MQ=255)
                              agaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGc                                                                                                                         >  1:2694214/1‑150 (MQ=255)
                              agaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGc                                                                                                                         >  2:1984618/1‑150 (MQ=255)
                                aagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGt                                                                                                                       >  2:3512392/1‑150 (MQ=255)
                                aagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGt                                                                                                                       >  2:4197387/1‑150 (MQ=255)
                                   ttATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAt                                                                                                                    >  1:4080733/1‑150 (MQ=255)
                                     ttCGATTTCTACAGCATAAACTAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAAGAGGCTGTTGAAGCTTTACTGGCGATTGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTTAttt                                                                                                                  <  1:198519/149‑1 (MQ=255)
                                     aTCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAttt                                                                                                                  >  2:3442010/1‑150 (MQ=255)
                                        gAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACg                                                                                                               <  2:3203708/150‑1 (MQ=255)
                                         aGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgc                                                                                                              >  1:1077983/1‑150 (MQ=255)
                                           ttCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgcag                                                                                                            <  1:4271307/150‑1 (MQ=255)
                                             cTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgcagca                                                                                                          >  2:2168052/1‑150 (MQ=255)
                                              tACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                         >  2:3506286/1‑150 (MQ=255)
                                               aCAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATa                                                                                                        <  1:181524/150‑1 (MQ=255)
                                                 aGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGCACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAc                                                                                                      <  2:1045/150‑1 (MQ=255)
                                                  gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAcg                                                                                                     <  1:4585399/150‑1 (MQ=255)
                                                      aaaCAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCt                                                                                                 >  2:897934/1‑150 (MQ=255)
                                                         cAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTaaa                                                                                              >  1:223583/1‑150 (MQ=255)
                                                          aaaGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAAt                                                                                             >  1:444981/1‑150 (MQ=255)
                                                           aaGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAAtt                                                                                            <  2:2351920/150‑1 (MQ=255)
                                                           aaGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAAtt                                                                                            >  1:805183/1‑150 (MQ=255)
                                                              aaCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGc                                                                                         >  2:4589135/1‑150 (MQ=255)
                                                               aCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGca                                                                                        >  2:1569765/1‑150 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       <  2:1454876/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       >  1:399585/1‑150 (MQ=255)
                                                                 tGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcaca                                                                                      <  1:3857963/150‑1 (MQ=255)
                                                                   aTGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACaaa                                                                                    >  2:513004/1‑150 (MQ=255)
                                                                    tGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAg                                                                                   <  2:2852863/150‑1 (MQ=255)
                                                                      gACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGc                                                                                 >  2:1089903/1‑150 (MQ=255)
                                                                        caacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGccc                                                                               >  1:3084928/1‑150 (MQ=255)
                                                                         aacaTGCGCAATGTTTCTCTGGAAGAACAGGCTGGTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTAACGCAGCATAACGCGCTAAAATCGCACAAAGGCCCg                                                                              >  2:714184/1‑150 (MQ=255)
                                                                         aacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCg                                                                              <  2:2942278/150‑1 (MQ=255)
                                                                               cgcAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCg                                                                        >  2:3677580/1‑150 (MQ=255)
                                                                                 cAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGcc                                                                      <  2:3555877/150‑1 (MQ=255)
                                                                                    tGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAgg                                                                   >  2:271703/1‑150 (MQ=255)
                                                                                      ttGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtg                                                                 >  2:574363/1‑150 (MQ=255)
                                                                                        gCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtggt                                                               >  1:3486116/1‑150 (MQ=255)
                                                                                         ctctGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGgtggtg                                                              <  1:2155674/150‑1 (MQ=255)
                                                                                            tGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGc                                                           >  2:2057202/1‑150 (MQ=255)
                                                                                              gaagaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtt                                                         <  1:3199442/150‑1 (MQ=255)
                                                                                               aagaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttt                                                        >  1:1626327/1‑150 (MQ=255)
                                                                                                  aaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtttttt                                                     >  1:2059398/1‑150 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttttttt                                                    <  1:3943462/150‑1 (MQ=255)
                                                                                                    cAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtttttttt                                                   <  2:393748/150‑1 (MQ=255)
                                                                                                     aGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTg                                                  >  1:4236262/1‑150 (MQ=255)
                                                                                                         tGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCAt                                              <  1:1652615/150‑1 (MQ=255)
                                                                                                             gAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAt                                          <  1:3656246/150‑1 (MQ=255)
                                                                                                                 cTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTg                                      >  2:112808/1‑150 (MQ=255)
                                                                                                                   gTACTGGCGAAAGCGTAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCa                                    <  1:1750669/150‑1 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   >  1:976181/1‑150 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   <  1:2307279/150‑1 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   >  1:4606541/1‑150 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   >  2:4439066/1‑150 (MQ=255)
                                                                                                                     aCTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCCCTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATg                                  <  1:3744426/150‑1 (MQ=255)
                                                                                                                     aCTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATg                                  <  1:620651/150‑1 (MQ=255)
                                                                                                                     aCTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATg                                  >  2:3893674/1‑150 (MQ=255)
                                                                                                                       tGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGa                                >  2:1996270/1‑150 (MQ=255)
                                                                                                                       tGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGa                                >  2:3125420/1‑150 (MQ=255)
                                                                                                                        ggCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGaa                               <  1:2935378/150‑1 (MQ=255)
                                                                                                                        ggCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGaa                               >  1:1322194/1‑150 (MQ=255)
                                                                                                                         gcgaaagcgaaaGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAc                              <  2:2532842/150‑1 (MQ=255)
                                                                                                                         gcgaaagcgaaaGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAc                              >  2:2600207/1‑150 (MQ=255)
                                                                                                                           gaaagcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCg                            >  1:2490580/1‑150 (MQ=255)
                                                                                                                              agcgaaagTTACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGc                         <  2:3580840/150‑1 (MQ=255)
                                                                                                                                cgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGTACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGa                       >  2:3132576/1‑150 (MQ=255)
                                                                                                                                cgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGa                       >  1:1172248/1‑150 (MQ=255)
                                                                                                                                 gaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaa                      <  1:1210153/150‑1 (MQ=255)
                                                                                                                                  aaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATTAATTTTGCATGGAACCGTGCGaaa                     <  2:3240140/150‑1 (MQ=255)
                                                                                                                                   aagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaaaa                    >  1:3505329/1‑150 (MQ=255)
                                                                                                                                    agTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAg                   <  2:679162/150‑1 (MQ=255)
                                                                                                                                      tGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGcc                 >  1:9445/1‑150 (MQ=255)
                                                                                                                                       gACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCct                >  1:1841564/1‑150 (MQ=255)
                                                                                                                                        aCTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCctc               <  1:1894487/150‑1 (MQ=255)
                                                                                                                                        aCTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCATGCGAAAAGCctc               <  2:3322833/150‑1 (MQ=255)
                                                                                                                                            aaaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc           >  1:3167541/1‑150 (MQ=255)
                                                                                                                                             aaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCg          <  2:3492547/150‑1 (MQ=255)
                                                                                                                                                aGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGgtg       >  1:63589/1‑150 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     >  2:337204/1‑150 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     <  2:1094833/150‑1 (MQ=255)
                                                                                                                                                   aaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTa    >  2:3658020/1‑150 (MQ=255)
                                                                                                                                                     ccACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGc  <  1:1528434/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGC  >  CP009273/451704‑452002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A