Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 451,853 C→A 15.4% T422N (ACC→AAC)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273451,8530CA15.4% 833.5 / 98.5 266T422N (ACC→AAC) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (111/114);  new base A (25/16);  total (136/130)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 274 total aligned reads displayed.

AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGC  >  CP009273/451704‑452002
                                                                                                                                                     |                                                                                                                                                     
agagATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAc                                                                                                                                                       <  1:1103551/150‑1 (MQ=255)
 gagaTGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAcc                                                                                                                                                      >  1:2088431/1‑150 (MQ=255)
  agaTGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCa                                                                                                                                                     >  2:4251453/1‑150 (MQ=255)
    aTGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACt                                                                                                                                                   >  2:2171997/1‑150 (MQ=255)
     tGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACtt                                                                                                                                                  <  2:489027/150‑1 (MQ=255)
         ttCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCaa                                                                                                                                              >  2:47166/1‑150 (MQ=255)
                 aCGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCt                                                                                                                                        >  2:1027453/1‑148 (MQ=255)
                 aCGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCt                                                                                                                                        <  1:1027453/148‑1 (MQ=255)
                  cGAAGATCCGAAAGAAGTTATCGATTTCTACAGCAAAAACAAAGAACTGTTGGACAACATGCGAAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCTAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCtgat                                                                                                                                     <  1:4081759/150‑1 (MQ=255)
                    aaGATCCGAACTAAGTTATCGAGTTCTACAGCAAAAACAACGAACTGATGGACAACTTGCGCAATGTTGCTCTGGAAGAACCGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGACACCACTTTCAACGAGCtgatga                                                                                                                                   <  1:3400279/150‑1 (MQ=255)
                    aaGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCtgatga                                                                                                                                   >  2:3933243/1‑150 (MQ=255)
                     aGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGaa                                                                                                                                  >  1:1440407/1‑150 (MQ=255)
                      gATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAAc                                                                                                                                 >  2:2905260/1‑150 (MQ=255)
                      gATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAAc                                                                                                                                 >  2:985968/1‑150 (MQ=255)
                       aTCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAAcc                                                                                                                                >  1:3774476/1‑150 (MQ=255)
                        tCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACca                                                                                                                               >  2:1503123/1‑150 (MQ=255)
                            aaaGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACcagcag                                                                                                                           >  2:3295018/1‑150 (MQ=255)
                             aagaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAgg                                                                                                                          >  1:885392/1‑150 (MQ=255)
                               gaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCg                                                                                                                        >  1:3214653/1‑150 (MQ=255)
                                aagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGt                                                                                                                       >  1:1904543/1‑150 (MQ=255)
                                aagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGt                                                                                                                       >  1:3085793/1‑150 (MQ=255)
                                   ttATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAt                                                                                                                    <  2:4257847/150‑1 (MQ=255)
                                    tATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCACTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAtt                                                                                                                   <  2:3765864/150‑1 (MQ=255)
                                    tATCGAGTTCAACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAtt                                                                                                                   >  1:726075/1‑150 (MQ=255)
                                      tCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACCGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTa                                                                                                                 <  2:2312860/150‑1 (MQ=255)
                                       cGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTAc                                                                                                                >  2:3381718/1‑150 (MQ=255)
                                        gAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACg                                                                                                               >  2:2431446/1‑150 (MQ=255)
                                          gTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgca                                                                                                             >  1:3979972/1‑150 (MQ=255)
                                           ttCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgcag                                                                                                            >  1:2331993/1‑150 (MQ=255)
                                             cTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgcagca                                                                                                          >  2:3784135/1‑150 (MQ=255)
                                             cTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgcagca                                                                                                          >  2:3904262/1‑150 (MQ=255)
                                              tACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                         <  1:3384803/150‑1 (MQ=255)
                                              tACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                         >  2:3981703/1‑150 (MQ=255)
                                              tACAGCAAAAACAAAGAACTGATGGACAACATGCGCAAGGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                         <  1:2974765/150‑1 (MQ=255)
                                              aaCAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTGGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                         <  1:2244350/149‑1 (MQ=255)
                                                 aGCAAAAACAACGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAc                                                                                                      <  1:497152/150‑1 (MQ=255)
                                                  gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAcg                                                                                                     <  2:4349064/150‑1 (MQ=255)
                                                  gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAcg                                                                                                     <  1:1716361/150‑1 (MQ=255)
                                                           aaGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAAtt                                                                                            <  2:499044/150‑1 (MQ=255)
                                                             gAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCg                                                                                          >  1:3200052/1‑150 (MQ=255)
                                                             gAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCg                                                                                          >  1:3501251/1‑150 (MQ=255)
                                                               aCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGca                                                                                        >  1:4021548/1‑150 (MQ=255)
                                                               aCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGca                                                                                        <  2:1655329/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       <  1:3134841/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       <  2:292172/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       >  2:1099836/1‑150 (MQ=255)
                                                                 tGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcaca                                                                                      >  1:2521593/1‑150 (MQ=255)
                                                                     ggACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAgg                                                                                  <  2:2088576/150‑1 (MQ=255)
                                                                      gACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGc                                                                                 <  2:3607169/150‑1 (MQ=255)
                                                                       acaacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGTAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGcc                                                                                <  2:4333942/150‑1 (MQ=255)
                                                                       acaacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGcc                                                                                >  1:276474/1‑150 (MQ=255)
                                                                       acaacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGcc                                                                                >  2:4092226/1‑150 (MQ=255)
                                                                        caacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGccc                                                                               <  1:4267074/150‑1 (MQ=255)
                                                                         aacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCg                                                                              <  2:1995098/150‑1 (MQ=255)
                                                                                 cAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGcc                                                                      >  1:3643539/1‑150 (MQ=255)
                                                                                  aaTGTTGCTCTGGAAGAACAGGCTGTTGAAACTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCa                                                                     >  2:1565289/1‑150 (MQ=255)
                                                                                   aTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAg                                                                    >  1:3417658/1‑150 (MQ=255)
                                                                                   aTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAg                                                                    <  2:3687873/150‑1 (MQ=255)
                                                                                     gTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggt                                                                  <  1:1545197/150‑1 (MQ=255)
                                                                                     gTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggt                                                                  >  1:1605105/1‑150 (MQ=255)
                                                                                      ttGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtg                                                                 <  1:1382217/150‑1 (MQ=255)
                                                                                       tGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtgg                                                                >  1:4068460/1‑150 (MQ=255)
                                                                                         ctctGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGgtggtg                                                              >  1:2657986/1‑150 (MQ=255)
                                                                                         ctctGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGgtggtg                                                              <  2:1794192/150‑1 (MQ=255)
                                                                                              gaagaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtt                                                         <  2:3591073/150‑1 (MQ=255)
                                                                                              gaagaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtt                                                         >  1:199689/1‑150 (MQ=255)
                                                                                               aagaaCAGGCTGTTGAAGCTGGACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttt                                                        <  2:2303013/150‑1 (MQ=255)
                                                                                                 gaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttttt                                                      <  1:571743/150‑1 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttttttt                                                    >  1:1172515/1‑150 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCttttttt                                                    <  2:3116566/150‑1 (MQ=255)
                                                                                                    cAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtttttttt                                                   <  2:1205778/150‑1 (MQ=255)
                                                                                                    cAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCtttttttt                                                   >  1:126511/1‑150 (MQ=255)
                                                                                                     aGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTg                                                  >  1:3151359/1‑150 (MQ=255)
                                                                                                           ttGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGa                                            >  1:2482972/1‑150 (MQ=255)
                                                                                                             gAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAt                                          >  1:4195527/1‑150 (MQ=255)
                                                                                                               aGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAttt                                        <  2:546773/150‑1 (MQ=255)
                                                                                                               aGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCACAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAttt                                        >  2:598848/1‑150 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   <  2:3766434/150‑1 (MQ=255)
                                                                                                                    tACTCGCGAAAGCGAAAGTGACTGAAAAAGAAACCACATACAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCAt                                   <  2:3465289/150‑1 (MQ=255)
                                                                                                                       tGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGa                                <  1:175119/150‑1 (MQ=255)
                                                                                                                          cgaaagcgaaagTGACTGAAAAAGAAACCTCTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCTCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAcc                             <  2:825567/150‑1 (MQ=255)
                                                                                                                          cgaaagcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAcc                             >  2:4174530/1‑150 (MQ=255)
                                                                                                                            aaagcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGt                           <  2:171850/150‑1 (MQ=255)
                                                                                                                             acgcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTg                          <  1:3581971/148‑1 (MQ=255)
                                                                                                                                cgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGa                       >  2:2080647/1‑150 (MQ=255)
                                                                                                                                 gaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaa                      >  1:2413389/1‑150 (MQ=255)
                                                                                                                                 gaaagTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaa                      <  1:2558120/150‑1 (MQ=255)
                                                                                                                                       gACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCct                >  2:3511783/1‑150 (MQ=255)
                                                                                                                                          tGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCtt             <  1:47166/150‑1 (MQ=255)
                                                                                                                                           gAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCttt            <  1:1649785/150‑1 (MQ=255)
                                                                                                                                            aaaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc           <  2:1307033/150‑1 (MQ=255)
                                                                                                                                                aGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGgtg       >  1:3380664/1‑150 (MQ=255)
                                                                                                                                                 gAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGgtgt      <  1:2919594/150‑1 (MQ=255)
                                                                                                                                                  aaaaCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     <  2:673295/150‑1 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGACAAGCCTCTTTCGGTGtt     >  2:2709293/1‑150 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     >  2:4071829/1‑150 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     >  2:1905128/1‑150 (MQ=255)
                                                                                                                                                  aaaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGtt     <  1:953610/150‑1 (MQ=255)
                                                                                                                                                    aCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAg   >  1:2809235/1‑150 (MQ=255)
                                                                                                                                                     ccACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGc  >  2:1451606/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGC  >  CP009273/451704‑452002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A