Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 451,853 C→A 32.8% T422N (ACC→AAC)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273451,8530CA32.8% 519.5 / 246.2 282T422N (ACC→AAC) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (86/103);  new base A (56/37);  total (142/140)
Fisher's exact test for biased strand distribution p-value = 2.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAG  >  CP009273/451704‑452001
                                                                                                                                                     |                                                                                                                                                     
    aTGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACt                                                                                                                                                   <  2:881563/150‑1 (MQ=255)
     tGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACtt                                                                                                                                                  >  1:3648416/1‑150 (MQ=255)
       gCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTc                                                                                                                                                <  1:3017402/150‑1 (MQ=255)
        cTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCa                                                                                                                                               >  2:894495/1‑150 (MQ=255)
        cTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCa                                                                                                                                               >  1:882938/1‑150 (MQ=255)
        cTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCa                                                                                                                                               <  1:325918/150‑1 (MQ=255)
             gCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAg                                                                                                                                          <  1:2994410/150‑1 (MQ=255)
              cGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGc                                                                                                                                         >  1:3090422/1‑150 (MQ=255)
                tACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCtg                                                                                                                                       >  2:1213564/1‑150 (MQ=255)
                 aCGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCtga                                                                                                                                      >  2:3947677/1‑150 (MQ=255)
                  cGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCtgat                                                                                                                                     <  2:961524/150‑1 (MQ=255)
                  cGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCtgat                                                                                                                                     >  1:505986/1‑150 (MQ=255)
                    aaGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCtgatga                                                                                                                                   <  2:413244/150‑1 (MQ=255)
                     aGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGaa                                                                                                                                  >  1:4068187/1‑150 (MQ=255)
                       aTCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAAcc                                                                                                                                >  1:1102904/1‑150 (MQ=255)
                        tCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACca                                                                                                                               <  2:613034/150‑1 (MQ=255)
                           gAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACcagca                                                                                                                            >  2:309253/1‑150 (MQ=255)
                            aaaGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACcagcag                                                                                                                           >  2:229729/1‑150 (MQ=255)
                             aagaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAgg                                                                                                                          >  2:2464980/1‑150 (MQ=255)
                               gaagTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCg                                                                                                                        >  2:477352/1‑150 (MQ=255)
                                 agTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTa                                                                                                                      <  2:1984622/150‑1 (MQ=255)
                                 agTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTa                                                                                                                      <  1:1432877/150‑1 (MQ=255)
                                  gTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTaa                                                                                                                     <  1:3880002/150‑1 (MQ=255)
                                    tATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAtt                                                                                                                   >  2:3030148/1‑150 (MQ=255)
                                     aTCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAAttt                                                                                                                  >  2:3075235/1‑150 (MQ=255)
                                          gTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACgca                                                                                                             <  2:580126/150‑1 (MQ=255)
                                           ttCTACAGCAAAAACAAAGAACTGATGGACACCATGCGCAATGTTGATCTGGATGAACAGGCTGTTGAAGCTGTTCTTGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTTATGAACCAGCAGGCGTAATTTACgcag                                                                                                            <  1:566379/150‑1 (MQ=255)
                                                 aGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAc                                                                                                      <  1:320920/150‑1 (MQ=255)
                                                 aGCAAAAACAAAGAACTGATGGACAACATGCGCAAGGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAc                                                                                                      <  1:3513333/150‑1 (MQ=255)
                                                  gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAcg                                                                                                     <  1:1679329/150‑1 (MQ=255)
                                                  gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAAcg                                                                                                     <  2:2167268/150‑1 (MQ=255)
                                                      aaaCAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCt                                                                                                 >  2:274308/1‑150 (MQ=255)
                                                        aCAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTATCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTaa                                                                                               >  1:366451/1‑150 (MQ=255)
                                                            aGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTc                                                                                           >  2:2381914/1‑150 (MQ=255)
                                                            aGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTc                                                                                           >  2:731153/1‑150 (MQ=255)
                                                               aCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGca                                                                                        <  1:690727/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       <  2:722058/150‑1 (MQ=255)
                                                                cTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcac                                                                                       >  1:2694902/1‑150 (MQ=255)
                                                                 tGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGcaca                                                                                      <  1:2948365/150‑1 (MQ=255)
                                                                   aTGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACaaa                                                                                    >  2:1288109/1‑150 (MQ=255)
                                                                      gACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGc                                                                                 >  1:1321552/1‑150 (MQ=255)
                                                                           caTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTc                                                                            >  2:158387/1‑150 (MQ=255)
                                                                            aTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCa                                                                           <  2:1091574/150‑1 (MQ=255)
                                                                             tGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCAc                                                                          <  1:3068809/150‑1 (MQ=255)
                                                                             tGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCAc                                                                          >  2:1671597/1‑150 (MQ=255)
                                                                             tGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCAc                                                                          <  1:933778/150‑1 (MQ=255)
                                                                              gcgcAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCAcc                                                                         <  2:2001074/150‑1 (MQ=255)
                                                                                 cAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGcc                                                                      >  2:812949/1‑150 (MQ=255)
                                                                                  aaggTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCa                                                                     <  2:3528370/147‑1 (MQ=255)
                                                                                  aaTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCa                                                                     <  1:977814/150‑1 (MQ=255)
                                                                                  aaTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCa                                                                     <  1:2728949/150‑1 (MQ=255)
                                                                                     gTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggt                                                                  <  1:398368/150‑1 (MQ=255)
                                                                                     gTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggt                                                                  >  1:2499767/1‑150 (MQ=255)
                                                                                      ttGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtg                                                                 <  2:1476015/150‑1 (MQ=255)
                                                                                        gCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAggtggt                                                               >  2:2957891/1‑150 (MQ=255)
                                                                                         ctctGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGgtggtg                                                              >  2:2364173/1‑150 (MQ=255)
                                                                                            tGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGc                                                           >  1:3297899/1‑150 (MQ=255)
                                                                                             ggAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑t                                                         <  2:3614115/150‑1 (MQ=255)
                                                                                              gaagaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑tt                                                        <  1:1379750/150‑1 (MQ=255)
                                                                                              gaagaaCAGGCTGTTGAAGATGTACTGGCGATAGCGAAAGTGTCTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGAACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑tt                                                        <  1:2924535/150‑1 (MQ=255)
                                                                                                 gaaCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑ttttt                                                     <  2:3546202/150‑1 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑ttttttt                                                   <  2:3488762/150‑1 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑ttttttt                                                   <  1:3398684/150‑1 (MQ=255)
                                                                                                   aCAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑ttttttt                                                   >  2:260948/1‑150 (MQ=255)
                                                                                                    cAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑tttttttt                                                  <  2:2477711/150‑1 (MQ=255)
                                                                                                       gCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTc                                               <  1:1747260/150‑1 (MQ=255)
                                                                                                        cTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCa                                              >  1:1072101/1‑150 (MQ=255)
                                                                                                          gTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATg                                            >  1:778477/1‑150 (MQ=255)
                                                                                                           ttGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGa                                           <  1:3734907/150‑1 (MQ=255)
                                                                                                               aGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAAttt                                       <  1:4006343/150‑1 (MQ=255)
                                                                                                                gCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAAtttt                                      <  1:1559388/150‑1 (MQ=255)
                                                                                                                 cTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTg                                     >  1:2381158/1‑150 (MQ=255)
                                                                                                                  tGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGc                                    >  2:3695222/1‑150 (MQ=255)
                                                                                                                   gTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCa                                   <  1:1989433/150‑1 (MQ=255)
                                                                                                                    tACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCAt                                  <  1:1955533/150‑1 (MQ=255)
                                                                                                                         gcgaaagcgaaaGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAAc                             <  1:2484741/150‑1 (MQ=255)
                                                                                                                         gcgaaagcgaaaGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAAc                             <  1:2485111/150‑1 (MQ=255)
                                                                                                                          cgaaagcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAAcc                            <  1:1407226/150‑1 (MQ=255)
                                                                                                                            aaagcgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGt                          <  1:3154628/150‑1 (MQ=255)
                                                                                                                              agcgaaagTGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGc                        >  2:1405022/1‑150 (MQ=255)
                                                                                                                                cgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGa                      >  2:162354/1‑150 (MQ=255)
                                                                                                                                cgaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGa                      <  1:1230054/150‑1 (MQ=255)
                                                                                                                                 gaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaa                     <  1:3926450/150‑1 (MQ=255)
                                                                                                                                 gaaagTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGaa                     >  2:1150585/1‑150 (MQ=255)
                                                                                                                                      tGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGcc                >  2:1248689/1‑150 (MQ=255)
                                                                                                                                      tGACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGcc                >  1:1846806/1‑150 (MQ=255)
                                                                                                                                       gACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCct               <  1:2822369/150‑1 (MQ=255)
                                                                                                                                       gACTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCct               >  2:3322661/1‑150 (MQ=255)
                                                                                                                                        aCTGAAAAAGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCctc              <  2:2054730/150‑1 (MQ=255)
                                                                                                                                         cTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCctct             <  2:403093/150‑1 (MQ=255)
                                                                                                                                          tGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCtt            <  2:3216245/150‑1 (MQ=255)
                                                                                                                                            aaaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc          <  1:3237381/150‑1 (MQ=255)
                                                                                                                                            aaaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc          <  1:1636182/150‑1 (MQ=255)
                                                                                                                                            aaaaaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc          <  2:2862162/150‑1 (MQ=255)
                                                                                                                                              aaaGAAAACACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCgg        >  1:898209/1‑150 (MQ=255)
                                                                                                                                               aaGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCgg        <  2:1268105/150‑1 (MQ=255)
                                                                                                                                                 gAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGgtgt     >  2:3563645/1‑150 (MQ=255)
                                                                                                                                                   aaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCCGGTGGTGGGC‑TTTTTTTGGTCATGAATTTTGCATGGACCCGTGCGAATAGCCTCTTGCGGTGTTa   >  2:2130018/1‑150 (MQ=255)
                                                                                                                                                   aaCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTa   <  1:3206046/150‑1 (MQ=255)
                                                                                                                                                    aCCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAg  <  1:4115877/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGC‑TTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAG  >  CP009273/451704‑452001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A