Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 678,325 G→T 16.7% G175G (GGC→GGA)  hscC ← Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273678,3250GT16.7% 768.0 / 85.9 243G175G (GGC→GGA) hscCHsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription
Reads supporting (aligned to +/- strand):  ref base G (97/104);  new base T (17/25);  total (114/129)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

AGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCT  >  CP009273/678176‑678474
                                                                                                                                                     |                                                                                                                                                     
aGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTgccg                                                                                                                                                       <  1:4599791/150‑1 (MQ=255)
  cGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTgccgcc                                                                                                                                                     >  1:615613/1‑150 (MQ=255)
   gTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCa                                                                                                                                                    >  2:1088721/1‑150 (MQ=255)
   gTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCa                                                                                                                                                    >  1:1523755/1‑150 (MQ=255)
     ggTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCAcc                                                                                                                                                  <  2:3016178/150‑1 (MQ=255)
     ggTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCAcc                                                                                                                                                  <  1:1495676/150‑1 (MQ=255)
     ggTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGATGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCAcc                                                                                                                                                  >  2:3777827/1‑150 (MQ=255)
         cTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCgaga                                                                                                                                              >  2:3238247/1‑150 (MQ=255)
          tGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGAt                                                                                                                                             <  2:4848/150‑1 (MQ=255)
            gCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCa                                                                                                                                           <  2:2386022/150‑1 (MQ=255)
             cGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaa                                                                                                                                          >  1:2171302/1‑150 (MQ=255)
              gACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaaa                                                                                                                                         >  1:458204/1‑150 (MQ=255)
              gACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaaa                                                                                                                                         >  1:4423468/1‑150 (MQ=255)
                cATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAaca                                                                                                                                       >  1:3375911/1‑150 (MQ=255)
                cATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAaca                                                                                                                                       <  2:4309355/150‑1 (MQ=255)
                   ccGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCa                                                                                                                                    <  2:3670849/150‑1 (MQ=255)
                    cgcgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCTAACGTGCCGCCACCGAGATCAAACACCAg                                                                                                                                   <  2:1537419/150‑1 (MQ=255)
                    cgcgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAg                                                                                                                                   <  1:1971858/150‑1 (MQ=255)
                      cgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCg                                                                                                                                 >  2:1089569/1‑150 (MQ=255)
                       gcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGa                                                                                                                                >  1:2052311/1‑150 (MQ=255)
                        cgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGaa                                                                                                                               >  2:2079440/1‑150 (MQ=255)
                             tAACACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGt                                                                                                                          <  2:59973/150‑1 (MQ=255)
                              aaaaCCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTa                                                                                                                         >  1:1351631/1‑150 (MQ=255)
                              aaaaCCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTa                                                                                                                         >  2:1755228/1‑150 (MQ=255)
                                      aTCGACCCGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTttgttg                                                                                                                 <  2:1367167/150‑1 (MQ=255)
                                      aTCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTttgttg                                                                                                                 <  2:254226/150‑1 (MQ=255)
                                       tCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTgg                                                                                                                <  1:4081504/150‑1 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               <  1:390254/150‑1 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               <  1:390672/150‑1 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               >  1:240412/1‑150 (MQ=255)
                                          aCCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa                                                                                                             >  2:3891661/1‑150 (MQ=255)
                                          aCCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa                                                                                                             >  1:1397598/1‑150 (MQ=255)
                                           ccAGCATATGGGTAAAATCTTCGCCACCAAGACAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTAt                                                                                                            <  2:1161923/150‑1 (MQ=255)
                                            cAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                           >  1:101180/1‑150 (MQ=255)
                                             aGCATATGGGTAAAATCTTCGCCACCAAGANAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGc                                                                                                          >  1:4282703/1‑150 (MQ=255)
                                               cATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACTTCAAACGTGCCGCCACCGAGATAAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAg                                                                                                        <  1:1866605/150‑1 (MQ=255)
                                               cATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAg                                                                                                        >  2:2958337/1‑150 (MQ=255)
                                                atatGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAgg                                                                                                       >  2:1911319/1‑150 (MQ=255)
                                                atatGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAgg                                                                                                       >  1:254108/1‑150 (MQ=255)
                                                atatGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAgg                                                                                                       >  2:3852225/1‑150 (MQ=255)
                                                  atGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGACAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGACCGGGTATTTTGTTGGGTATGCAGGcc                                                                                                     >  2:4626050/1‑150 (MQ=255)
                                                    gggTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTATGTTGGGTATGCAGGACAt                                                                                                   >  2:36869/1‑150 (MQ=255)
                                                    gggTAAAATCTACGCCACCAAGAAAGTCGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGCACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCAt                                                                                                   <  2:2841482/150‑1 (MQ=255)
                                                       tAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACg                                                                                                >  1:1410281/1‑150 (MQ=255)
                                                            tCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATc                                                                                           <  1:1062880/150‑1 (MQ=255)
                                                            tCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATc                                                                                           <  1:441073/150‑1 (MQ=255)
                                                             cTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCg                                                                                          >  1:3995333/1‑150 (MQ=255)
                                                                 gCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCACCGAACGGGGAGTTTGTTGGGTATGCTGGCCATACGCCATCGCAgc                                                                                      >  2:2220689/1‑150 (MQ=255)
                                                                  ccaccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgct                                                                                     <  2:885666/150‑1 (MQ=255)
                                                                  ccaccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgct                                                                                     <  1:720318/150‑1 (MQ=255)
                                                                   caccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctg                                                                                    >  1:4191620/1‑150 (MQ=255)
                                                                   caccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctg                                                                                    >  1:2411031/1‑150 (MQ=255)
                                                                     ccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctgct                                                                                  >  2:4120205/1‑150 (MQ=255)
                                                                       aaGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCtgt                                                                                >  1:3429741/1‑150 (MQ=255)
                                                                         gAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTgg                                                                              <  1:1625312/150‑1 (MQ=255)
                                                                           aaGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGt                                                                            >  1:411018/1‑150 (MQ=255)
                                                                            aGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGtt                                                                           <  1:354400/150‑1 (MQ=255)
                                                                            aGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGtt                                                                           <  1:2767772/150‑1 (MQ=255)
                                                                             gTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACTTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTc                                                                          <  2:1304748/150‑1 (MQ=255)
                                                                             gTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTc                                                                          <  1:4447069/150‑1 (MQ=255)
                                                                                gTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatt                                                                       <  1:1901048/150‑1 (MQ=255)
                                                                                   gCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCattaat                                                                    <  2:3812570/150‑1 (MQ=255)
                                                                                    ccAGCCGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCattaatt                                                                   <  2:2591084/150‑1 (MQ=255)
                                                                                     cAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCattaatta                                                                  >  2:1627789/1‑150 (MQ=255)
                                                                                       gcggcggcgTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAg                                                                <  2:2718545/150‑1 (MQ=255)
                                                                                       gCGGAGGCGTGAACTTCACTCACCGGCGTGGCGGACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAg                                                                <  2:2718425/150‑1 (MQ=255)
                                                                                       gCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCGGCTGCTGTGGGTTCATTAATTAAg                                                                <  1:3617208/150‑1 (MQ=255)
                                                                                       gCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAg                                                                <  1:2671226/150‑1 (MQ=255)
                                                                                         ggAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCg                                                              >  1:4469623/1‑150 (MQ=255)
                                                                                           aGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACTTATAACGTGCCTCCACCGTGTTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTa                                                            <  2:4531222/150‑1 (MQ=255)
                                                                                              cGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCg                                                         <  2:2018134/150‑1 (MQ=255)
                                                                                              cGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCg                                                         >  1:833438/1‑150 (MQ=255)
                                                                                                tGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGAAACGTCAAACGTGACGCCACCGAGATCAAACAAAAGCGAACGGGTCATTTGTTGGGAATGCAGGCCAAACTCCATCGCAGATGCAGTGGGTTCATAAATTAAGCGTACCGCa                                                       >  2:2166258/1‑150 (MQ=255)
                                                                                                  aaCTTCACTCACCGGCGTGGCGTACTCTATCACCGTAACGTCAAACGTGCCTACACCGAGATCAAACACCAGCGAACTGGTATTTTGTTGGGTATGCAGGCCATACTCCATCGCAGCTGCTGTGGGTTCATTAATTCAGCGTACCGCAtt                                                     <  1:1802111/150‑1 (MQ=255)
                                                                                                  aaCTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCAtt                                                     >  2:2826273/1‑150 (MQ=255)
                                                                                                   aCTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCAttt                                                    <  1:4189719/150‑1 (MQ=255)
                                                                                                    cTTCACTCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTa                                                   <  2:352065/150‑1 (MQ=255)
                                                                                                     ttCACTCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTaa                                                  <  2:4233577/150‑1 (MQ=255)
                                                                                                      tCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                 <  1:291839/150‑1 (MQ=255)
                                                                                                      tCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                 <  1:1439811/150‑1 (MQ=255)
                                                                                                          tCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCgg                                             >  2:4471780/1‑150 (MQ=255)
                                                                                                           cACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGc                                            >  1:2494025/1‑150 (MQ=255)
                                                                                                             ccGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTa                                          <  2:3375911/150‑1 (MQ=255)
                                                                                                              cGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTaa                                         >  2:2469839/1‑150 (MQ=255)
                                                                                                              cGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTaa                                         <  1:1089569/150‑1 (MQ=255)
                                                                                                               ggcgtggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAAc                                        <  1:2958337/150‑1 (MQ=255)
                                                                                                                gcgtggcgtACTCAAGCACCGTATCGTCTAACGTGCCGCCACCGAGATCATACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACt                                       <  2:1502762/150‑1 (MQ=255)
                                                                                                                 cgtggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTc                                      <  2:1404979/150‑1 (MQ=255)
                                                                                                                      cgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCgctg                                 >  1:3318262/1‑150 (MQ=255)
                                                                                                                        tACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCgctgct                               <  2:472981/150‑1 (MQ=255)
                                                                                                                          cTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTaa                             >  2:3592655/1‑150 (MQ=255)
                                                                                                                            cAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAAc                           >  1:3274638/1‑150 (MQ=255)
                                                                                                                                cACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGt                       >  2:3946924/1‑150 (MQ=255)
                                                                                                                                    gTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGc                   >  2:1629610/1‑150 (MQ=255)
                                                                                                                                     tAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCt                  >  1:4535089/1‑150 (MQ=255)
                                                                                                                                     tAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCt                  >  1:1299112/1‑150 (MQ=255)
                                                                                                                                      aaCGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCtt                 >  2:78106/1‑150 (MQ=255)
                                                                                                                                           cAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCgtt            <  1:515153/150‑1 (MQ=255)
                                                                                                                                               cGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTc        <  1:4245589/150‑1 (MQ=255)
                                                                                                                                               cGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTc        <  2:1682749/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCT  >  CP009273/678176‑678474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A