Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 679,765 G→T 41.7% S35Y (TCT→TAT)  rihA ← ribonucleoside hydrolase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273679,7650GT41.7% 303.5 / inf 275S35Y (TCT→TAT) rihAribonucleoside hydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (81/79);  new base T (67/48);  total (148/127)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

TTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAG  >  CP009273/679616‑679913
                                                                                                                                                      |                                                                                                                                                    
tttCGCCGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAg                                                                                                                                                      <  2:1609307/150‑1 (MQ=255)
    gCCGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGt                                                                                                                                                  >  1:3908845/1‑150 (MQ=255)
    gCCGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGt                                                                                                                                                  >  1:1306515/1‑150 (MQ=255)
     ccGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTa                                                                                                                                                 >  1:952777/1‑150 (MQ=255)
      cGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTaa                                                                                                                                                >  1:894349/1‑150 (MQ=255)
       gTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAAt                                                                                                                                               <  2:4115827/150‑1 (MQ=255)
        tGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAAtt                                                                                                                                              >  2:3723915/1‑150 (MQ=255)
          cacaTTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGc                                                                                                                                            <  2:3319395/150‑1 (MQ=255)
            caTTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCtt                                                                                                                                          <  2:2785220/150‑1 (MQ=255)
             aTTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCttt                                                                                                                                         <  2:2151414/150‑1 (MQ=255)
                gTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGAc                                                                                                                                      >  2:2915005/1‑150 (MQ=255)
                gTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGAc                                                                                                                                      >  1:2010688/1‑150 (MQ=255)
                  ccGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACAt                                                                                                                                    <  1:1175135/150‑1 (MQ=255)
                     cgATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCaa                                                                                                                                 >  2:2753638/1‑150 (MQ=255)
                     cgATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCaa                                                                                                                                 >  1:326668/1‑150 (MQ=255)
                      gATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAg                                                                                                                                >  2:1347684/1‑150 (MQ=255)
                      gATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAg                                                                                                                                >  2:3568086/1‑150 (MQ=255)
                          aTCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGctct                                                                                                                            <  1:3696274/150‑1 (MQ=255)
                             aaCTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGt                                                                                                                         <  1:2757556/150‑1 (MQ=255)
                              aCTCACGCATTAACGGTTTTACCGCGCCGCCAGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTg                                                                                                                        <  2:3782169/150‑1 (MQ=255)
                              aCTCACGCAATAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTg                                                                                                                        <  2:3580785/150‑1 (MQ=255)
                               cTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGa                                                                                                                       <  1:1504062/150‑1 (MQ=255)
                               cTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGTTGa                                                                                                                       <  1:1923963/150‑1 (MQ=255)
                                tCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAg                                                                                                                      <  2:978780/150‑1 (MQ=255)
                                 cACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAgg                                                                                                                     <  2:3954668/150‑1 (MQ=255)
                                   cGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAggcg                                                                                                                   >  2:2298409/1‑150 (MQ=255)
                                      atcaaCGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAggcgagg                                                                                                                >  2:219138/4‑150 (MQ=255)
                                      aTTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAggcgagg                                                                                                                >  2:3176039/1‑150 (MQ=255)
                                      aTTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAggcgagg                                                                                                                <  1:3728554/150‑1 (MQ=255)
                                          aCGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAggcgagggcga                                                                                                            <  2:3039087/150‑1 (MQ=255)
                                            ggTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCgaga                                                                                                          >  1:2659073/1‑150 (MQ=255)
                                            ggTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCgaga                                                                                                          <  1:4155294/150‑1 (MQ=255)
                                             gTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGaa                                                                                                         >  1:2445727/1‑150 (MQ=255)
                                                 tACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTAt                                                                                                     >  2:2614127/1‑150 (MQ=255)
                                                   ccGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTg                                                                                                   <  2:386425/150‑1 (MQ=255)
                                                   ccGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTg                                                                                                   <  1:1835762/150‑1 (MQ=255)
                                                    cgcgCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGc                                                                                                  >  2:2420325/1‑150 (MQ=255)
                                                      cgccgcCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATGGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGa                                                                                                >  2:12059/1‑150 (MQ=255)
                                                       gccgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGAt                                                                                               <  1:2914551/150‑1 (MQ=255)
                                                       gccgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGAt                                                                                               >  2:3398877/1‑150 (MQ=255)
                                                        ccgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGAt                                                                                               >  1:1650249/1‑150 (MQ=255)
                                                        ccgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATa                                                                                              <  1:1693201/150‑1 (MQ=255)
                                                         cgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAg                                                                                             <  2:902831/150‑1 (MQ=255)
                                                              gCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGcgtcg                                                                                        >  2:1816484/1‑150 (MQ=255)
                                                                   cGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATg                                                                                   <  2:3223738/150‑1 (MQ=255)
                                                                   cGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATg                                                                                   >  1:4140076/1‑150 (MQ=255)
                                                                   cGGAATATCGGTGAGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑ATTTTACTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATg                                                                                   <  1:1356738/150‑1 (MQ=255)
                                                                     gAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGAc                                                                                 >  1:3772101/1‑150 (MQ=255)
                                                                       atatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCt                                                                               <  2:1645200/150‑1 (MQ=255)
                                                                       atatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCt                                                                               <  2:1645212/150‑1 (MQ=255)
                                                                       atatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCt                                                                               >  2:2266923/1‑150 (MQ=255)
                                                                           cGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGt                                                                           <  2:2819639/150‑1 (MQ=255)
                                                                            ggTTCGATTAAGCAAGGTCAGAATACGCAGAACATTTCGTAATG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAATTATTGCGATAGCGTCGTCATGACCTGGGTc                                                                          <  1:3860539/150‑1 (MQ=255)
                                                                            ggTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTc                                                                          >  2:2545311/1‑150 (MQ=255)
                                                                            ggTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTc                                                                          <  1:4042509/150‑1 (MQ=255)
                                                                            ggTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGAAATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTc                                                                          >  2:2094530/1‑150 (MQ=255)
                                                                                 gATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAAt                                                                     >  1:2277557/1‑150 (MQ=255)
                                                                                  aTTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATc                                                                    >  2:1311065/1‑150 (MQ=255)
                                                                                  aTTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATc                                                                    >  1:1778261/1‑150 (MQ=255)
                                                                                     aaGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTaa                                                                 >  1:597995/1‑150 (MQ=255)
                                                                                      aGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAAc                                                                <  2:192213/150‑1 (MQ=255)
                                                                                      aGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGAGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGAGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAAc                                                                <  1:300539/150‑1 (MQ=255)
                                                                                         aaGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGa                                                             >  1:758991/1‑150 (MQ=255)
                                                                                         aaGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGa                                                             >  2:558412/1‑150 (MQ=255)
                                                                                           ggTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAAt                                                           >  2:713321/1‑150 (MQ=255)
                                                                                             tCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTg                                                         >  1:3460596/1‑150 (MQ=255)
                                                                                                gCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCa                                                      <  1:1545044/150‑1 (MQ=255)
                                                                                                  aTACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGt                                                    <  1:3483108/150‑1 (MQ=255)
                                                                                                     cGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGcc                                                 <  2:1490729/150‑1 (MQ=255)
                                                                                                        aGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCAtt                                              >  2:2387727/1‑150 (MQ=255)
                                                                                                          aaCATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCAttgt                                            <  2:2517828/150‑1 (MQ=255)
                                                                                                            ctttGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACTTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCAttgttg                                          <  1:810689/148‑1 (MQ=255)
                                                                                                                  gTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCtt                                    <  1:203088/150‑1 (MQ=255)
                                                                                                                   tAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttg                                   >  1:4051716/1‑150 (MQ=255)
                                                                                                                      gg‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttgttg                                <  1:2459301/150‑1 (MQ=255)
                                                                                                                      gg‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttgttg                                >  1:3297536/1‑150 (MQ=255)
                                                                                                                           ttttCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTtgtgtg                            <  2:1196007/150‑1 (MQ=255)
                                                                                                                           ttttCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTtgtgtg                            >  2:693512/1‑150 (MQ=255)
                                                                                                                              tCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGctt                         <  1:3240100/150‑1 (MQ=255)
                                                                                                                               cTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                        <  1:2091147/150‑1 (MQ=255)
                                                                                                                               cTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                        >  1:3543864/1‑150 (MQ=255)
                                                                                                                               cTGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                        >  1:959248/1‑150 (MQ=255)
                                                                                                                                tGGTGTCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttct                       <  1:3623920/150‑1 (MQ=255)
                                                                                                                                  gtgtCTGGTTTCCGGCGGAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCttt                     >  2:3138053/1‑150 (MQ=255)
                                                                                                                                   tgtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTg                    <  1:3062220/150‑1 (MQ=255)
                                                                                                                                    gtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGc                   >  1:2554471/1‑150 (MQ=255)
                                                                                                                                     tCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCg                  >  2:2294886/1‑150 (MQ=255)
                                                                                                                                       tGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGAc                <  1:384622/150‑1 (MQ=255)
                                                                                                                                       tGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGAc                <  1:384833/150‑1 (MQ=255)
                                                                                                                                        ggTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACa               <  1:170899/150‑1 (MQ=255)
                                                                                                                                         gTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACaa              >  2:312201/1‑150 (MQ=255)
                                                                                                                                          tttCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAg             <  1:2633436/150‑1 (MQ=255)
                                                                                                                                           ttCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAgg            <  2:644969/150‑1 (MQ=255)
                                                                                                                                             ccGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGt          >  1:1265351/1‑150 (MQ=255)
                                                                                                                                             ccGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGt          >  1:3231801/1‑150 (MQ=255)
                                                                                                                                              cggcggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTa         <  1:2820966/150‑1 (MQ=255)
                                                                                                                                              cggcggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTa         >  2:3685166/1‑150 (MQ=255)
                                                                                                                                              cggcggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTa         <  1:1517101/150‑1 (MQ=255)
                                                                                                                                                  ggAATACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGc     >  1:3380033/1‑150 (MQ=255)
                                                                                                                                                   gAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGcc    >  1:656726/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTTCGCCGTGCACATTGTCCGCGATAATCAACTCACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGG‑TTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAG  >  CP009273/679616‑679913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A