Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 678,325 G→T 40.3% G175G (GGC→GGA)  hscC ← Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273678,3250GT40.3% 326.3 / 303.4 268G175G (GGC→GGA) hscCHsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription
Reads supporting (aligned to +/- strand):  ref base G (66/94);  new base T (51/57);  total (117/151)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 278 total aligned reads displayed.

AGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCT  >  CP009273/678176‑678474
                                                                                                                                                     |                                                                                                                                                     
aGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTgccg                                                                                                                                                       <  2:1805923/150‑1 (MQ=255)
   gTGGTCCTGGCGACATCCGCGCGATTTAAACCCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCa                                                                                                                                                    <  2:3455358/150‑1 (MQ=255)
    tGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCAc                                                                                                                                                   <  2:2093504/150‑1 (MQ=255)
    tGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCAc                                                                                                                                                   <  1:554884/150‑1 (MQ=255)
    tGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCAc                                                                                                                                                   >  2:1644108/1‑150 (MQ=255)
     ggTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCAcc                                                                                                                                                  <  1:4570217/150‑1 (MQ=255)
        ccTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCgag                                                                                                                                               <  1:3730766/150‑1 (MQ=255)
        ccTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCgag                                                                                                                                               >  1:84632/1‑150 (MQ=255)
         cTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCgaga                                                                                                                                              <  2:3303267/150‑1 (MQ=255)
         cTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCgaga                                                                                                                                              >  2:3436804/1‑150 (MQ=255)
          tGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGAt                                                                                                                                             <  1:1713932/150‑1 (MQ=255)
             cGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaa                                                                                                                                          >  1:4541636/1‑150 (MQ=255)
             cGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaa                                                                                                                                          >  1:3940571/1‑150 (MQ=255)
              gACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCaaa                                                                                                                                         >  1:2674509/1‑150 (MQ=255)
                cATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAaca                                                                                                                                       <  1:4450608/150‑1 (MQ=255)
                   ccGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCa                                                                                                                                    <  1:1472927/150‑1 (MQ=255)
                    cgcgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAg                                                                                                                                   >  2:1019500/1‑150 (MQ=255)
                         gTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAAc                                                                                                                              <  1:2328981/150‑1 (MQ=255)
                         gTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAAc                                                                                                                              >  2:802380/1‑150 (MQ=255)
                         gTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAAc                                                                                                                              <  2:3042653/150‑1 (MQ=255)
                          atttAAAACCTCCTCGACCCGCATATGGGTAAAATCTACGCCACCAAGACAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAATTACAAACGTGCCTCCACCGATATCAAACACCAGCGAACg                                                                                                                             <  2:586671/149‑1 (MQ=255)
                           tttAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACgg                                                                                                                            <  1:1267067/150‑1 (MQ=255)
                           tctcaaaCCTCCTCGACCAGCATATGGGTAAAATCTCCGCCACCACGAACGCTGTCGCCAGCGGAGGCGTGAACTTCAATCCCCGGCGTGGCGTACTCAAGCACCGTATCGTCAAACGTGCCGCCACCGTGATCATACACCAGCGAACgg                                                                                                                            <  2:3941009/146‑1 (MQ=255)
                              aaaaCCTCCTCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACTTCAAACGTGCCGCCACCGTGATCAAACACCAGCGAACGGGTa                                                                                                                         <  2:905025/150‑1 (MQ=255)
                              aaaaCCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTa                                                                                                                         <  1:946711/150‑1 (MQ=255)
                               aaaCCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTAt                                                                                                                        >  1:1612399/1‑150 (MQ=255)
                                  ccTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTAtttt                                                                                                                     <  1:4293093/150‑1 (MQ=255)
                                   cTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTttg                                                                                                                    >  1:3603930/1‑150 (MQ=255)
                                        cGACCAGCATATGGGTACAATCTTCGCCACCCAGACCGTAGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               <  2:1480178/150‑1 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCCAGACAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               <  2:2374708/150‑1 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               >  2:3727034/1‑150 (MQ=255)
                                        cGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTggg                                                                                                               <  1:1899347/150‑1 (MQ=255)
                                          aCCAGCATCTGGGTAAAATCTTCGCCACCAAGCAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa                                                                                                             <  2:3366454/150‑1 (MQ=255)
                                          aCCAGCATATGGGTAACATCTTCGCCACCACGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCCCCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTa                                                                                                             <  2:930572/150‑1 (MQ=255)
                                            cAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                           >  2:4556195/1‑150 (MQ=255)
                                            cAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                           <  1:3585532/150‑1 (MQ=255)
                                            cAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                           <  1:4072669/150‑1 (MQ=255)
                                                  atGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGcc                                                                                                     >  2:3355543/1‑150 (MQ=255)
                                                   tGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCa                                                                                                    <  1:2587024/150‑1 (MQ=255)
                                                    gggTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCAt                                                                                                   <  1:3498926/150‑1 (MQ=255)
                                                        aaaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGc                                                                                               >  2:1028995/1‑150 (MQ=255)
                                                        aaaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGc                                                                                               >  2:1551108/1‑150 (MQ=255)
                                                         aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATATGcc                                                                                              >  2:4178310/1‑150 (MQ=255)
                                                         aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTAAGCAGGCCATACGcc                                                                                              >  2:1298117/1‑150 (MQ=255)
                                                         aaaTCTTCGCCAACAATAAAGATTTCGCCATCTGAGGCGTTAACTTAAATCACCGGCGTGGCGTACTCAAGCACCGTAACTTAAAACGAGAAGCCACCCATATAAAACAAAAGCGAACGTGTATATTGTTGGGTATTCAAGACATGCGcc                                                                                              >  2:1298222/1‑150 (MQ=255)
                                                            tcgtCGCCACCACGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCCCCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATc                                                                                           <  2:838750/147‑1 (MQ=255)
                                                              ttCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGc                                                                                         >  1:1517874/1‑150 (MQ=255)
                                                                cGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAg                                                                                       <  2:3205751/150‑1 (MQ=255)
                                                                 gCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgc                                                                                      >  1:463509/1‑150 (MQ=255)
                                                                 gCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgc                                                                                      <  1:1646943/150‑1 (MQ=255)
                                                                  ccaccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgct                                                                                     <  1:2568360/150‑1 (MQ=255)
                                                                  ccaccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGGTGGGTATGCAGGCCATACGCCATCGCAgct                                                                                     >  1:734652/1‑150 (MQ=255)
                                                                   caccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctg                                                                                    <  2:2749076/150‑1 (MQ=255)
                                                                     ccaAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctgct                                                                                  <  1:2904569/150‑1 (MQ=255)
                                                                       aaGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCtgt                                                                                <  2:4118406/150‑1 (MQ=255)
                                                                       aaGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCtgt                                                                                <  2:2993156/150‑1 (MQ=255)
                                                                       aaGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCtgt                                                                                >  2:2756060/1‑150 (MQ=255)
                                                                        aGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCtgtg                                                                               >  1:2196747/1‑150 (MQ=255)
                                                                         gAAAGTTGTCGCCAGCGGAGGCGTGACCTTCCACCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTgg                                                                              <  2:2527917/150‑1 (MQ=255)
                                                                             gTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTc                                                                          <  1:3466730/150‑1 (MQ=255)
                                                                              ttGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAAAGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCa                                                                         >  1:1161533/1‑150 (MQ=255)
                                                                                 tCGCCAGCGGAGGCGTGAACTTCACTCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatta                                                                      <  2:1245412/150‑1 (MQ=255)
                                                                                 tCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatta                                                                      <  1:2130748/150‑1 (MQ=255)
                                                                                   gCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCattaat                                                                    <  2:1865834/150‑1 (MQ=255)
                                                                                      aGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCAttaattaa                                                                 >  1:634438/1‑150 (MQ=255)
                                                                                      aGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCAttaattaa                                                                 >  2:1713425/1‑150 (MQ=255)
                                                                                      aGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCAttaattaa                                                                 >  2:1420899/1‑150 (MQ=255)
                                                                                            ggCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTAc                                                           <  1:2697263/150‑1 (MQ=255)
                                                                                               gTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTCTTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGc                                                        >  2:2151195/1‑150 (MQ=255)
                                                                                               gTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTAGTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGc                                                        >  2:733395/1‑150 (MQ=255)
                                                                                                tGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCa                                                       >  1:1298120/1‑150 (MQ=255)
                                                                                                tGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCa                                                       >  1:1867411/1‑150 (MQ=255)
                                                                                                tGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCa                                                       <  1:2812224/150‑1 (MQ=255)
                                                                                                 gAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCAt                                                      >  2:4439138/1‑150 (MQ=255)
                                                                                                   aCTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCAttt                                                    <  2:1226075/150‑1 (MQ=255)
                                                                                                      tcactcacCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGTGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                 <  2:1363165/150‑1 (MQ=255)
                                                                                                      tCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCTAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                 <  2:1047749/150‑1 (MQ=255)
                                                                                                        aaTCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAccc                                               <  1:3304065/150‑1 (MQ=255)
                                                                                                             ccGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTa                                          <  1:182813/150‑1 (MQ=255)
                                                                                                                 cgtggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTc                                      <  1:3330117/150‑1 (MQ=255)
                                                                                                                   tggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCg                                    <  1:327546/150‑1 (MQ=255)
                                                                                                                   tggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCg                                    <  1:3009537/150‑1 (MQ=255)
                                                                                                                      cgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCgctg                                 >  1:1351276/1‑150 (MQ=255)
                                                                                                                       gtACTCAAGCACCGTAACGTCAAACGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCgctgc                                >  1:3754919/1‑150 (MQ=255)
                                                                                                                       gtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGGGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACACCgctgc                                >  2:1009825/1‑150 (MQ=255)
                                                                                                                                 aCCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTa                      >  2:2368531/1‑150 (MQ=255)
                                                                                                                                    gTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGc                   >  2:1340736/1‑150 (MQ=255)
                                                                                                                                      aaCGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCtt                 <  1:101637/150‑1 (MQ=255)
                                                                                                                                       acgactaaCGTGCCGCCACCGATATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTg                <  2:2478519/144‑1 (MQ=255)
                                                                                                                                         gTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCg              <  2:564891/150‑1 (MQ=255)
                                                                                                                                           cAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCgtt            <  1:3699273/150‑1 (MQ=255)
                                                                                                                                            aaaCGTGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCgttg           >  2:3033524/1‑150 (MQ=255)
                                                                                                                                            aaaCGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCgttg           <  2:3145332/150‑1 (MQ=255)
                                                                                                                                             aaCGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCgttgt          <  2:3607686/150‑1 (MQ=255)
                                                                                                                                                gTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCa       >  2:2225134/1‑150 (MQ=255)
                                                                                                                                                gTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCa       >  1:2925613/1‑150 (MQ=255)
                                                                                                                                                 tGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCAt      >  2:2495184/1‑150 (MQ=255)
                                                                                                                                                 tGCCTCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCAt      >  2:2896344/1‑150 (MQ=255)
                                                                                                                                                  gcctccACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATc     >  1:143207/1‑150 (MQ=255)
                                                                                                                                                   ccgccACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCg    <  1:1717883/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCT  >  CP009273/678176‑678474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A