Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,220,996 G→T 100% R181R (CGG→AGG)  minC ← cell division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,220,9960GT98.3% 891.7 / 4.2 263R181R (CGG→AGG) minCcell division inhibitor
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (129/129);  total (132/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 263 total aligned reads displayed.

ACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGC  >  CP009273/1220849‑1221141
                                                                                                                                                   |                                                                                                                                                 
accgTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGt                                                                                                                                                 >  1:2502636/4‑150 (MQ=255)
  ggTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGTACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTca                                                                                                                                               <  2:3743856/150‑1 (MQ=255)
   gTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcac                                                                                                                                              <  2:2079512/150‑1 (MQ=255)
    ttGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcacc                                                                                                                                             <  2:3307834/150‑1 (MQ=255)
      gAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcaccac                                                                                                                                           >  2:1005644/1‑150 (MQ=255)
        aCGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACtt                                                                                                                                         <  2:2598182/150‑1 (MQ=255)
        aCGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACtt                                                                                                                                         <  2:4026317/150‑1 (MQ=255)
           gTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGcc                                                                                                                                      >  2:483289/1‑150 (MQ=255)
           gTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGcc                                                                                                                                      >  2:4455951/1‑150 (MQ=255)
            tCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGccc                                                                                                                                     >  1:4555516/1‑150 (MQ=255)
              aaaGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCt                                                                                                                                   >  1:1015058/1‑150 (MQ=255)
                accGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGc                                                                                                                                 >  2:2222216/3‑150 (MQ=255)
                aGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGc                                                                                                                                 >  1:406090/1‑150 (MQ=255)
                       tCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCa                                                                                                                          <  1:3949708/150‑1 (MQ=255)
                        cGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCAc                                                                                                                         <  1:4175830/150‑1 (MQ=255)
                        cGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCAc                                                                                                                         >  2:2699568/1‑150 (MQ=255)
                           cTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAc                                                                                                                      >  1:3368574/1‑150 (MQ=255)
                            tAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAcc                                                                                                                     >  1:2186774/1‑150 (MQ=255)
                            tAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAcc                                                                                                                     <  1:3143750/150‑1 (MQ=255)
                             aaCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcg                                                                                                                    >  2:3585601/1‑150 (MQ=255)
                             aaCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcg                                                                                                                    >  2:4499253/1‑150 (MQ=255)
                             aaCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcg                                                                                                                    >  2:3497747/1‑150 (MQ=255)
                              aCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcgc                                                                                                                   <  2:3615697/150‑1 (MQ=255)
                               cTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcgcg                                                                                                                  <  2:4183394/150‑1 (MQ=255)
                                   aGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatc                                                                                                              >  1:845095/1‑150 (MQ=255)
                                    gTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatca                                                                                                             <  1:3424617/150‑1 (MQ=255)
                                      cgcgCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATg                                                                                                           >  1:4347436/1‑150 (MQ=255)
                                         gccgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCa                                                                                                        >  2:3887857/1‑150 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                >  2:429792/1‑150 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                >  2:1441468/1‑150 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATAACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                <  1:2895042/150‑1 (MQ=255)
                                                  ccATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGa                                                                                               >  2:2917701/1‑150 (MQ=255)
                                                    aTAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAt                                                                                             >  1:2942320/1‑150 (MQ=255)
                                                     tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATg                                                                                            <  2:878419/150‑1 (MQ=255)
                                                      aaaaTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGt                                                                                           >  1:2562530/1‑150 (MQ=255)
                                                         aTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTcc                                                                                        <  1:2429708/150‑1 (MQ=255)
                                                         aTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTcc                                                                                        <  1:3551509/150‑1 (MQ=255)
                                                           tCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCa                                                                                      <  1:2192100/150‑1 (MQ=255)
                                                           tCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCa                                                                                      <  1:3989114/150‑1 (MQ=255)
                                                             tgctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATc                                                                                    >  2:1223546/1‑150 (MQ=255)
                                                              gctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCg                                                                                   <  2:678585/150‑1 (MQ=255)
                                                              gctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCg                                                                                   >  1:1916274/1‑150 (MQ=255)
                                                               ctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCgg                                                                                  >  2:926147/1‑150 (MQ=255)
                                                                tgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                 <  1:268456/150‑1 (MQ=255)
                                                                    aTTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatc                                                                             >  1:3770283/1‑150 (MQ=255)
                                                                       tGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                          <  2:3680618/150‑1 (MQ=255)
                                                                       tGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                          <  2:1974512/150‑1 (MQ=255)
                                                                        gATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCCTAGACATGAATGTTCCCATCGGCAATCAAtt                                                                         >  2:820425/1‑150 (MQ=255)
                                                                         aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTc                                                                        <  1:3653375/150‑1 (MQ=255)
                                                                          tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCg                                                                       >  2:1237148/1‑150 (MQ=255)
                                                                          tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCg                                                                       <  2:1329908/150‑1 (MQ=255)
                                                                           cACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCgg                                                                      <  1:3504595/150‑1 (MQ=255)
                                                                             cTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcc                                                                    <  2:1870836/150‑1 (MQ=255)
                                                                               cAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcccc                                                                  <  1:2016070/150‑1 (MQ=255)
                                                                                    aGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAgcgc                                                             <  1:1864280/150‑1 (MQ=255)
                                                                                     gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                            <  1:1651023/150‑1 (MQ=255)
                                                                                     gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                            >  1:2629453/1‑150 (MQ=255)
                                                                                     gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                            <  2:148369/150‑1 (MQ=255)
                                                                                     gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                            >  1:1155369/1‑150 (MQ=255)
                                                                                       aTTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTaa                                                          <  1:932191/150‑1 (MQ=255)
                                                                                       aTTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTaa                                                          >  1:2215847/1‑150 (MQ=255)
                                                                                           aCCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTg                                                      <  2:1488493/150‑1 (MQ=255)
                                                                                            ccTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTgg                                                     >  2:3080643/1‑150 (MQ=255)
                                                                                              tGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCt                                                   <  1:1336844/150‑1 (MQ=255)
                                                                                                 gATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGt                                                >  2:1758375/1‑150 (MQ=255)
                                                                                                  aTGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTa                                               <  1:4050718/150‑1 (MQ=255)
                                                                                                       cacCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAAGAAt                                          <  2:4180792/150‑1 (MQ=255)
                                                                                                       cacCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAt                                          <  1:475172/150‑1 (MQ=255)
                                                                                                        acCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATc                                         <  2:2333792/150‑1 (MQ=255)
                                                                                                           aGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGa                                      >  1:3041548/1‑150 (MQ=255)
                                                                                                           aGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGa                                      >  1:838985/1‑150 (MQ=255)
                                                                                                              tCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATca                                   <  1:271902/150‑1 (MQ=255)
                                                                                                              tCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATca                                   <  1:1803491/150‑1 (MQ=255)
                                                                                                                  ccATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAtt                               >  2:2047611/1‑150 (MQ=255)
                                                                                                                  ccATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAtt                               <  2:3280360/150‑1 (MQ=255)
                                                                                                                   cATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtg                              >  2:1722198/1‑150 (MQ=255)
                                                                                                                   cATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtg                              <  2:1180543/150‑1 (MQ=255)
                                                                                                                    aTCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtgt                             <  1:201410/150‑1 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCCGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                          >  1:503145/1‑150 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                          <  2:459299/150‑1 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                          <  2:178429/150‑1 (MQ=255)
                                                                                                                        ggTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAg                         >  2:1654054/1‑150 (MQ=255)
                                                                                                                         gTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGc                        >  2:4583655/1‑150 (MQ=255)
                                                                                                                             gTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATaa                    <  2:4097070/150‑1 (MQ=255)
                                                                                                                               aCAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAAt                  <  2:641820/150‑1 (MQ=255)
                                                                                                                               aCAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAAt                  <  2:641202/150‑1 (MQ=255)
                                                                                                                                cAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATa                 >  2:3149682/1‑150 (MQ=255)
                                                                                                                                cAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATa                 >  2:3094151/1‑150 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                >  1:4405490/1‑150 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                >  1:1191909/1‑150 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                >  1:4405437/1‑150 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                <  1:2960602/150‑1 (MQ=255)
                                                                                                                                  aaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACg               >  1:3446410/1‑150 (MQ=255)
                                                                                                                                    atatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCt             >  1:2741633/1‑150 (MQ=255)
                                                                                                                                      atTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGa           >  1:420333/1‑150 (MQ=255)
                                                                                                                                          gCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCgg       >  2:2364491/1‑150 (MQ=255)
                                                                                                                                           cGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa      <  1:3139224/150‑1 (MQ=255)
                                                                                                                                             tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAc    <  2:3960490/150‑1 (MQ=255)
                                                                                                                                              ttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACg   >  2:1260828/1‑150 (MQ=255)
                                                                                                                                              ttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACg   >  2:1260575/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGC  >  CP009273/1220849‑1221141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACC  >  CP009273/1220847‑1221144
                                                                                                                                                     |                                                                                                                                                    
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCT                                                                                                                                                      <  SRR19973072.2927193/150‑1 (MQ=60)
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCT                                                                                                                                                      <  SRR19973072.2926875/150‑1 (MQ=60)
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCT                                                                                                                                                      <  SRR19973072.3682555/150‑1 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                     >  SRR19973072.3330560/1‑150 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                     <  SRR19973072.3763511/150‑1 (MQ=60)
  ACCGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGT                                                                                                                                                    >  SRR19973072.2507993/1‑150 (MQ=60)
  ACCGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGT                                                                                                                                                    >  SRR19973072.2508078/1‑150 (MQ=60)
      TTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACC                                                                                                                                                >  SRR19973072.4360192/1‑150 (MQ=60)
       TGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCA                                                                                                                                               >  SRR19973072.4452997/1‑150 (MQ=60)
           CGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTG                                                                                                                                           >  SRR19973072.2742038/1‑150 (MQ=60)
           CGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTG                                                                                                                                           <  SRR19973072.1608164/150‑1 (MQ=60)
            GGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGC                                                                                                                                          <  SRR19973072.2702017/150‑1 (MQ=60)
              TCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCC                                                                                                                                        >  SRR19973072.4565112/1‑150 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                      <  SRR19973072.2140138/150‑1 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                      <  SRR19973072.2140075/150‑1 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                      >  SRR19973072.1017265/1‑150 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                      >  SRR19973072.1017283/1‑150 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                      >  SRR19973072.916976/1‑150 (MQ=60)
                  AGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGC                                                                                                                                    >  SRR19973072.406992/1‑150 (MQ=60)
                     GTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAG                                                                                                                                 >  SRR19973072.3086174/1‑150 (MQ=60)
                     GTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAG                                                                                                                                 >  SRR19973072.3507235/1‑150 (MQ=60)
                      TTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGC                                                                                                                                >  SRR19973072.3277972/1‑150 (MQ=60)
                         TCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCA                                                                                                                             <  SRR19973072.3957956/150‑1 (MQ=60)
                          CGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCAC                                                                                                                            >  SRR19973072.1869392/1‑150 (MQ=60)
                          CGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCAC                                                                                                                            <  SRR19973072.4184572/150‑1 (MQ=60)
                             CTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAC                                                                                                                         >  SRR19973072.3375711/1‑150 (MQ=60)
                             CTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAC                                                                                                                         >  SRR19973072.1746439/1‑150 (MQ=60)
                             CTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAC                                                                                                                         >  SRR19973072.2540973/1‑150 (MQ=60)
                              TAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACC                                                                                                                        <  SRR19973072.3150380/150‑1 (MQ=60)
                              TAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACC                                                                                                                        <  SRR19973072.3379474/150‑1 (MQ=60)
                              TAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACC                                                                                                                        <  SRR19973072.3150314/150‑1 (MQ=60)
                              TAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACC                                                                                                                        >  SRR19973072.2191529/1‑150 (MQ=60)
                                     AGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATC                                                                                                                 >  SRR19973072.846916/1‑150 (MQ=60)
                                     AGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATC                                                                                                                 >  SRR19973072.846911/1‑150 (MQ=60)
                                      GTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCA                                                                                                                <  SRR19973072.3431825/150‑1 (MQ=60)
                                      GTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCA                                                                                                                <  SRR19973072.3431935/150‑1 (MQ=60)
                                        CGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATG                                                                                                              >  SRR19973072.4356550/1‑150 (MQ=60)
                                        CGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATG                                                                                                              >  SRR19973072.4356083/1‑150 (MQ=60)
                                              GCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAG                                                                                                        <  SRR19973072.906149/150‑1 (MQ=60)
                                              GCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAG                                                                                                        <  SRR19973072.3103702/150‑1 (MQ=60)
                                               CTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGA                                                                                                       <  SRR19973072.2224849/150‑1 (MQ=60)
                                               CTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGA                                                                                                       <  SRR19973072.4562589/150‑1 (MQ=60)
                                                TTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAC                                                                                                      <  SRR19973072.788136/150‑1 (MQ=60)
                                                   GCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAACATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATG                                                                                                   <  SRR19973072.2901279/150‑1 (MQ=60)
                                                   GCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATG                                                                                                   <  SRR19973072.2901394/150‑1 (MQ=60)
                                                   GCCATAAAATTCTGCTGGGATTTGATAACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATG                                                                                                   <  SRR19973072.2901244/150‑1 (MQ=60)
                                                     CATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAA                                                                                                 >  SRR19973072.4248764/1‑150 (MQ=60)
                                                      ATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAT                                                                                                >  SRR19973072.2948613/1‑150 (MQ=60)
                                                       TAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATG                                                                                               <  SRR19973072.985757/150‑1 (MQ=60)
                                                       TAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATG                                                                                               >  SRR19973072.219504/1‑150 (MQ=60)
                                                        AAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGT                                                                                              >  SRR19973072.2568081/1‑150 (MQ=60)
                                                        AAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGT                                                                                              >  SRR19973072.2568319/1‑150 (MQ=60)
                                                        AAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGT                                                                                              <  SRR19973072.4030818/150‑1 (MQ=60)
                                                           ATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCC                                                                                           <  SRR19973072.2434976/150‑1 (MQ=60)
                                                           ATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCC                                                                                           <  SRR19973072.3558998/150‑1 (MQ=60)
                                                           ATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCC                                                                                           <  SRR19973072.3558969/150‑1 (MQ=60)
                                                             TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                         <  SRR19973072.2509931/150‑1 (MQ=60)
                                                             TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                         <  SRR19973072.3997439/150‑1 (MQ=60)
                                                             TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                         <  SRR19973072.3854562/150‑1 (MQ=60)
                                                             TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                         <  SRR19973072.3997462/150‑1 (MQ=60)
                                                             TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                         <  SRR19973072.2196871/150‑1 (MQ=60)
                                                               TGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATC                                                                                       <  SRR19973072.1749763/150‑1 (MQ=60)
                                                                GCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATATACATGAATGTTCCCATCG                                                                                      >  SRR19973072.1920351/1‑150 (MQ=60)
                                                                GCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCG                                                                                      <  SRR19973072.3705960/150‑1 (MQ=60)
                                                                GCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCG                                                                                      >  SRR19973072.3377635/1‑150 (MQ=60)
                                                                GCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCG                                                                                      >  SRR19973072.1920808/1‑150 (MQ=60)
                                                                 CTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGG                                                                                     >  SRR19973072.2960214/1‑150 (MQ=60)
                                                                  TGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGC                                                                                    <  SRR19973072.269090/150‑1 (MQ=60)
                                                                      ATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATC                                                                                <  SRR19973072.1852595/150‑1 (MQ=60)
                                                                      ATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATC                                                                                >  SRR19973072.3778191/1‑150 (MQ=60)
                                                                          GATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATT                                                                            >  SRR19973072.945913/1‑150 (MQ=60)
                                                                           ATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTC                                                                           <  SRR19973072.3661077/150‑1 (MQ=60)
                                                                            TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACGTGAATGTTCCCATCGGCAATCAATTCG                                                                          >  SRR19973072.2209390/1‑150 (MQ=60)
                                                                            TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                          >  SRR19973072.3628884/1‑150 (MQ=60)
                                                                             CACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGG                                                                         <  SRR19973072.3511939/150‑1 (MQ=60)
                                                                                 CAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCC                                                                     <  SRR19973072.2020422/150‑1 (MQ=60)
                                                                                   GCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAG                                                                   <  SRR19973072.1225131/150‑1 (MQ=60)
                                                                                      AGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGC                                                                <  SRR19973072.1868301/150‑1 (MQ=60)
                                                                                       GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                               >  SRR19973072.1157776/1‑150 (MQ=60)
                                                                                       GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                               >  SRR19973072.2635102/1‑150 (MQ=60)
                                                                                       GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                               <  SRR19973072.1654634/150‑1 (MQ=60)
                                                                                       GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                               >  SRR19973072.2635137/1‑150 (MQ=60)
                                                                                        TATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTA                                                              <  SRR19973072.3966011/150‑1 (MQ=60)
                                                                                         ATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAA                                                             <  SRR19973072.4151747/150‑1 (MQ=60)
                                                                                         ATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAA                                                             <  SRR19973072.934224/150‑1 (MQ=60)
                                                                                         ATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAA                                                             >  SRR19973072.2220661/1‑150 (MQ=60)
                                                                                          TTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAAC                                                            >  SRR19973072.2258671/1‑150 (MQ=60)
                                                                                                TGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCT                                                      <  SRR19973072.1339766/150‑1 (MQ=60)
                                                                                                 GCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTT                                                     <  SRR19973072.2207851/150‑1 (MQ=60)
                                                                                                   GATGGACAACAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGT                                                   >  SRR19973072.3518190/1‑150 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                  <  SRR19973072.1176573/150‑1 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                  <  SRR19973072.4059220/150‑1 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                  <  SRR19973072.4059227/150‑1 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                  <  SRR19973072.4199113/150‑1 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                  <  SRR19973072.1176557/150‑1 (MQ=60)
                                                                                                         CACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAT                                             <  SRR19973072.476286/150‑1 (MQ=60)
                                                                                                         CACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAT                                             >  SRR19973072.3463571/1‑150 (MQ=60)
                                                                                                          ACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATC                                            >  SRR19973072.4627924/1‑150 (MQ=60)
                                                                                                          ACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATC                                            <  SRR19973072.488313/150‑1 (MQ=60)
                                                                                                           CCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCA                                           <  SRR19973072.4378059/150‑1 (MQ=60)
                                                                                                             AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                         >  SRR19973072.840791/1‑150 (MQ=60)
                                                                                                             AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                         >  SRR19973072.3047987/1‑150 (MQ=60)
                                                                                                                TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                      <  SRR19973072.1807430/150‑1 (MQ=60)
                                                                                                                TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                      <  SRR19973072.272540/150‑1 (MQ=60)
                                                                                                                TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                      <  SRR19973072.4143453/150‑1 (MQ=60)
                                                                                                                   GCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAT                                   <  SRR19973072.316532/150‑1 (MQ=60)
                                                                                                                      ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                                <  SRR19973072.201925/150‑1 (MQ=60)
                                                                                                                      ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                                <  SRR19973072.201913/150‑1 (MQ=60)
                                                                                                                      ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATAGGAAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                                >  SRR19973072.821326/1‑150 (MQ=60)
                                                                                                                       TCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTG                               >  SRR19973072.3031045/1‑150 (MQ=60)
                                                                                                                        CAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTCTAACAATCAGATCACATTGTGG                              >  SRR19973072.4052534/1‑150 (MQ=60)
                                                                                                                         AGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCCGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGA                             >  SRR19973072.504343/1‑150 (MQ=60)
                                                                                                                          GGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAG                            >  SRR19973072.3752271/1‑150 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                   >  SRR19973072.4414758/1‑150 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                   >  SRR19973072.4414720/1‑150 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                   >  SRR19973072.4414705/1‑150 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                   >  SRR19973072.1194433/1‑150 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                   <  SRR19973072.2966923/150‑1 (MQ=60)
                                                                                                                                    AAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACG                  >  SRR19973072.3453641/1‑150 (MQ=60)
                                                                                                                                     AATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGAGGCTTGTAACAATCAGATCACATTGAGGAGCATAAATACGC                 >  SRR19973072.2573350/1‑150 (MQ=60)
                                                                                                                                      ATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCT                >  SRR19973072.2747493/1‑150 (MQ=60)
                                                                                                                                       TATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCCTGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTG               <  SRR19973072.2801394/150‑1 (MQ=60)
                                                                                                                                       TATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTG               <  SRR19973072.2801217/150‑1 (MQ=60)
                                                                                                                                       TATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTG               <  SRR19973072.4640172/150‑1 (MQ=60)
                                                                                                                                        ATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGA              >  SRR19973072.421281/1‑150 (MQ=60)
                                                                                                                                          TTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACC            <  SRR19973072.2705310/150‑1 (MQ=60)
                                                                                                                                             CGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGA         <  SRR19973072.1895963/150‑1 (MQ=60)
                                                                                                                                             CGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGA         <  SRR19973072.1929868/150‑1 (MQ=60)
                                                                                                                                             CGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGA         <  SRR19973072.3145845/150‑1 (MQ=60)
                                                                                                                                             CGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGA         <  SRR19973072.301692/150‑1 (MQ=60)
                                                                                                                                              GTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAA        <  SRR19973072.1351725/150‑1 (MQ=60)
                                                                                                                                                   CCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC   <  SRR19973072.453764/150‑1 (MQ=60)
                                                                                                                                                    CTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACC  >  SRR19973072.4573400/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACC  >  CP009273/1220847‑1221144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑