Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,220,996 G→T 100% R181R (CGG→AGG)  minC ← cell division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,220,9960GT100.0% 531.4 / NA 160R181R (CGG→AGG) minCcell division inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (83/77);  total (83/77)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 160 total aligned reads displayed.

GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACG  >  CP009273/1220851‑1221140
                                                                                                                                                 |                                                                                                                                                
  ttGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTca                                                                                                                                              >  1:2126382/1‑148 (MQ=255)
       cGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACt                                                                                                                                         <  2:257076/148‑1 (MQ=255)
       cGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCAAATAATTCTGCTGCGATAACATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACt                                                                                                                                         >  1:3249688/1‑148 (MQ=255)
          tCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGc                                                                                                                                      >  1:336676/1‑148 (MQ=255)
             aaGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGcccc                                                                                                                                   <  2:1108733/148‑1 (MQ=255)
              aGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCt                                                                                                                                  >  1:2458154/1‑148 (MQ=255)
               gCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTg                                                                                                                                 >  1:2499341/1‑148 (MQ=255)
               gCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTg                                                                                                                                 >  1:1252548/1‑148 (MQ=255)
                 gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGcc                                                                                                                               >  2:2183308/1‑148 (MQ=255)
                   tttCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                             >  1:626367/1‑148 (MQ=255)
                      cGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAgcgc                                                                                                                          >  2:2975715/1‑148 (MQ=255)
                      cGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAgcgc                                                                                                                          <  1:1692823/148‑1 (MQ=255)
                       gACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCa                                                                                                                         <  1:557778/148‑1 (MQ=255)
                                  gTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcat                                                                                                              <  1:3015082/148‑1 (MQ=255)
                                  gTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcat                                                                                                              <  1:3015080/148‑1 (MQ=255)
                                    cgcgCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatca                                                                                                            <  2:1801439/148‑1 (MQ=255)
                                    cgcgCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatca                                                                                                            <  2:980611/148‑1 (MQ=255)
                                       gccgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGc                                                                                                         <  2:3313839/148‑1 (MQ=255)
                                       gccgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGc                                                                                                         <  2:1352271/148‑1 (MQ=255)
                                         cgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCa                                                                                                       <  2:25301/148‑1 (MQ=255)
                                         cgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCa                                                                                                       >  1:789810/1‑148 (MQ=255)
                                            tttGCCATAACATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAg                                                                                                    <  2:1309908/148‑1 (MQ=255)
                                             ttGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGa                                                                                                   >  1:2228689/1‑148 (MQ=255)
                                              tGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAc                                                                                                  <  2:1857758/148‑1 (MQ=255)
                                                ccATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACAt                                                                                                >  2:2659120/1‑148 (MQ=255)
                                                   tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGaa                                                                                             <  2:2034791/148‑1 (MQ=255)
                                                   tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGaa                                                                                             >  1:1669888/1‑148 (MQ=255)
                                                   tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGaa                                                                                             >  1:3307112/1‑148 (MQ=255)
                                                     aaaTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATg                                                                                           <  2:1478631/148‑1 (MQ=255)
                                                       aTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGtt                                                                                         >  2:2131733/1‑148 (MQ=255)
                                                         tCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTcc                                                                                       <  2:2598558/148‑1 (MQ=255)
                                                         tCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTcc                                                                                       >  2:1682078/1‑148 (MQ=255)
                                                                ggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                >  1:1331844/1‑148 (MQ=255)
                                                                 gATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGca                                                                               >  2:795827/1‑148 (MQ=255)
                                                                  aTTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                              >  2:1503653/1‑148 (MQ=255)
                                                                  aTTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                              >  1:1323649/1‑148 (MQ=255)
                                                                  aTTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                              <  2:1089508/148‑1 (MQ=255)
                                                                   tttGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaat                                                                             <  1:709205/148‑1 (MQ=255)
                                                                   tttGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaat                                                                             <  1:709237/148‑1 (MQ=255)
                                                                      gATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaa                                                                          <  1:552608/148‑1 (MQ=255)
                                                                      gATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaa                                                                          <  1:2145792/148‑1 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         >  2:293060/1‑148 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         >  2:2235800/1‑148 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         >  2:2739165/1‑148 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         <  1:1114961/148‑1 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         <  1:1672209/148‑1 (MQ=255)
                                                                       aTCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaat                                                                         <  1:2766247/148‑1 (MQ=255)
                                                                        tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAAtt                                                                        <  1:667889/148‑1 (MQ=255)
                                                                        tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAAtt                                                                        <  1:282204/148‑1 (MQ=255)
                                                                             cAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcc                                                                   >  2:1009127/1‑148 (MQ=255)
                                                                             cAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcc                                                                   >  2:3207576/1‑148 (MQ=255)
                                                                              aGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                  <  2:2353435/148‑1 (MQ=255)
                                                                              aGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                  <  2:1843922/148‑1 (MQ=255)
                                                                               gCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcccc                                                                 >  1:2589663/1‑148 (MQ=255)
                                                                                  aGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAgc                                                              >  2:1356583/1‑148 (MQ=255)
                                                                                   gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAgcg                                                             <  1:2975049/148‑1 (MQ=255)
                                                                                     aTTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                           >  1:1005370/1‑148 (MQ=255)
                                                                                       tCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTaa                                                         >  2:1182915/1‑148 (MQ=255)
                                                                                           cTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTg                                                     >  1:2478592/1‑148 (MQ=255)
                                                                                            tGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTgg                                                    >  1:1278893/1‑148 (MQ=255)
                                                                                                   gACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                             <  2:843413/148‑1 (MQ=255)
                                                                                                      acCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACaa                                          <  2:1290344/148‑1 (MQ=255)
                                                                                                      acCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACaa                                          <  2:1226062/148‑1 (MQ=255)
                                                                                                       ccAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAt                                         >  1:3234792/1‑148 (MQ=255)
                                                                                                       ccAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAt                                         >  1:3235380/1‑148 (MQ=255)
                                                                                                       ccAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAt                                         <  2:2228689/148‑1 (MQ=255)
                                                                                                           ttCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGa                                     >  1:1297770/1‑148 (MQ=255)
                                                                                                           ttCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGa                                     >  1:2611762/1‑148 (MQ=255)
                                                                                                            tCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                    <  1:2661061/148‑1 (MQ=255)
                                                                                                               gCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATcac                                 <  2:2431793/148‑1 (MQ=255)
                                                                                                                ccATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATcaca                                <  1:2834718/148‑1 (MQ=255)
                                                                                                                  aTCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAtt                              <  1:1356583/148‑1 (MQ=255)
                                                                                                                    cAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtgt                            >  1:231619/1‑148 (MQ=255)
                                                                                                                        ttCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAg                        <  2:1550398/148‑1 (MQ=255)
                                                                                                                        ttCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAg                        >  2:593816/1‑148 (MQ=255)
                                                                                                                        ttCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAg                        >  2:370202/1‑148 (MQ=255)
                                                                                                                        ttCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAg                        >  2:370199/1‑148 (MQ=255)
                                                                                                                         tCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGc                       <  2:3040487/148‑1 (MQ=255)
                                                                                                                             aCAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATaa                   >  2:3076391/1‑148 (MQ=255)
                                                                                                                              cAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATaaa                  <  1:2183308/148‑1 (MQ=255)
                                                                                                                               aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAAt                 <  1:80284/148‑1 (MQ=255)
                                                                                                                               aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAAt                 >  2:2525860/1‑148 (MQ=255)
                                                                                                                                aaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATa                >  1:172917/1‑148 (MQ=255)
                                                                                                                                  atatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACg              >  2:1772364/1‑148 (MQ=255)
                                                                                                                                    atTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCt            >  2:906743/1‑148 (MQ=255)
                                                                                                                                      gtGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGa          <  2:1262537/147‑1 (MQ=255)
                                                                                                                                       tGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAc         >  2:400382/1‑148 (MQ=255)
                                                                                                                                       tGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAc         >  2:2062982/1‑148 (MQ=255)
                                                                                                                                         cGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCg       >  1:1647123/1‑148 (MQ=255)
                                                                                                                                         cGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCg       >  1:1368591/1‑148 (MQ=255)
                                                                                                                                           tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa     <  2:34082/148‑1 (MQ=255)
                                                                                                                                           tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa     <  2:1787108/148‑1 (MQ=255)
                                                                                                                                           tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa     <  2:2908931/148‑1 (MQ=255)
                                                                                                                                           tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa     <  2:22654/148‑1 (MQ=255)
                                                                                                                                           tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGa     >  2:2198665/1‑148 (MQ=255)
                                                                                                                                            ttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa    >  1:669406/1‑148 (MQ=255)
                                                                                                                                            ttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa    >  2:3066599/1‑148 (MQ=255)
                                                                                                                                            ttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAGTGTGGAGCATAAATACGCTGACCGGaa    >  2:1421246/1‑148 (MQ=255)
                                                                                                                                             tCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAc   >  1:2717177/1‑148 (MQ=255)
                                                                                                                                              cccTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTCACGTGGCTTGTAACAATCAGATCACAGTGTGGAGCATAAATACGCTGACCTGAACg  >  2:1698092/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACG  >  CP009273/1220851‑1221140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC  >  CP009273/1220850‑1221143
                                                                                                                                                  |                                                                                                                                                   
CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTC                                                                                                                                                  >  SRR19973070.1299340/1‑150 (MQ=60)
CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTC                                                                                                                                                  >  SRR19973070.2958369/1‑150 (MQ=60)
 GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCA                                                                                                                                                 <  SRR19973070.2589197/150‑1 (MQ=60)
 GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCA                                                                                                                                                 >  SRR19973070.2140636/1‑150 (MQ=60)
 GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCA                                                                                                                                                 >  SRR19973070.2140607/1‑150 (MQ=60)
 GGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCA                                                                                                                                                 <  SRR19973070.3006578/150‑1 (MQ=60)
      AACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCAAATAATTCTGCTGCGATAACATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACT                                                                                                                                            >  SRR19973070.3270632/1‑150 (MQ=60)
         GGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGC                                                                                                                                         >  SRR19973070.339130/1‑150 (MQ=60)
             AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACCCCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                     >  SRR19973070.2474248/1‑150 (MQ=60)
             AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                     >  SRR19973070.2474217/1‑150 (MQ=60)
              AAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTG                                                                                                                                    >  SRR19973070.3078871/1‑150 (MQ=60)
              AAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTG                                                                                                                                    >  SRR19973070.2515558/1‑150 (MQ=60)
              AAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTG                                                                                                                                    >  SRR19973070.1261334/1‑150 (MQ=60)
                  GTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAG                                                                                                                                >  SRR19973070.630796/1‑150 (MQ=60)
                       CGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCAC                                                                                                                           <  SRR19973070.1704462/150‑1 (MQ=60)
                        GACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACG                                                                                                                          <  SRR19973070.561448/150‑1 (MQ=60)
                                 CAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCAT                                                                                                                 <  SRR19973070.527739/150‑1 (MQ=60)
                                   GTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCA                                                                                                               <  SRR19973070.3034315/150‑1 (MQ=60)
                                   GTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCA                                                                                                               <  SRR19973070.3034317/150‑1 (MQ=60)
                                        GCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCNATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCA                                                                                                          <  SRR19973070.1691502/150‑1 (MQ=60)
                                        GCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCA                                                                                                          >  SRR19973070.2097190/1‑150 (MQ=60)
                                        GCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCA                                                                                                          >  SRR19973070.795286/1‑150 (MQ=60)
                                        GCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCA                                                                                                          >  SRR19973070.795316/1‑150 (MQ=60)
                                            CTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGA                                                                                                      >  SRR19973070.2243354/1‑150 (MQ=60)
                                                  CATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAA                                                                                                >  SRR19973070.1681348/1‑150 (MQ=60)
                                                  CATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAA                                                                                                >  SRR19973070.3328280/1‑150 (MQ=60)
                                                          TCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCA                                                                                        <  SRR19973070.2217689/150‑1 (MQ=60)
                                                               TGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGC                                                                                   >  SRR19973070.1341024/1‑150 (MQ=60)
                                                                 GGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAA                                                                                 >  SRR19973070.1332799/1‑150 (MQ=60)
                                                                    TTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCA                                                                              <  SRR19973070.714265/150‑1 (MQ=60)
                                                                    TTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCA                                                                              <  SRR19973070.714298/150‑1 (MQ=60)
                                                                    TTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCA                                                                              <  SRR19973070.714297/150‑1 (MQ=60)
                                                                       GATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATT                                                                           <  SRR19973070.2160095/150‑1 (MQ=60)
                                                                       GATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATT                                                                           <  SRR19973070.556267/150‑1 (MQ=60)
                                                                        ATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTC                                                                          <  SRR19973070.2783965/150‑1 (MQ=60)
                                                                        ATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTC                                                                          <  SRR19973070.1122578/150‑1 (MQ=60)
                                                                        ATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTC                                                                          <  SRR19973070.1683673/150‑1 (MQ=60)
                                                                         TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                         <  SRR19973070.672657/150‑1 (MQ=60)
                                                                         TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                         <  SRR19973070.672584/150‑1 (MQ=60)
                                                                         TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                         <  SRR19973070.284065/150‑1 (MQ=60)
                                                                              CAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCC                                                                    >  SRR19973070.2606586/1‑150 (MQ=60)
                                                                                    GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                              >  SRR19973070.1012535/1‑150 (MQ=60)
                                                                                    GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                              <  SRR19973070.1141258/150‑1 (MQ=60)
                                                                                    GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                              <  SRR19973070.2994151/150‑1 (MQ=60)
                                                                                       TTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAAC                                                           <  SRR19973070.58736/150‑1 (MQ=60)
                                                                                          ACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTG                                                        <  SRR19973070.3265399/150‑1 (MQ=60)
                                                                                          ACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTG                                                        <  SRR19973070.2416820/150‑1 (MQ=60)
                                                                                          ACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTG                                                        >  SRR19973070.2494733/1‑150 (MQ=60)
                                                                                           CCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGG                                                       >  SRR19973070.1287826/1‑150 (MQ=60)
                                                                                                      CACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAT                                            >  SRR19973070.3256263/1‑150 (MQ=60)
                                                                                                      CACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAT                                            >  SRR19973070.3255671/1‑150 (MQ=60)
                                                                                                       ACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATC                                           >  SRR19973070.3325503/1‑150 (MQ=60)
                                                                                                          AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                        >  SRR19973070.2628803/1‑150 (MQ=60)
                                                                                                          AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                        >  SRR19973070.2816309/1‑150 (MQ=60)
                                                                                                          AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                        >  SRR19973070.1306806/1‑150 (MQ=60)
                                                                                                          AGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGA                                        >  SRR19973070.1306894/1‑150 (MQ=60)
                                                                                                             TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                     <  SRR19973070.2678363/150‑1 (MQ=60)
                                                                                                             TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                     <  SRR19973070.2676415/150‑1 (MQ=60)
                                                                                                                 CCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATT                                 <  SRR19973070.2853061/150‑1 (MQ=60)
                                                                                                                 CCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATT                                 <  SRR19973070.1479075/150‑1 (MQ=60)
                                                                                                                   ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                               >  SRR19973070.233254/1‑150 (MQ=60)
                                                                                                                   ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                               <  SRR19973070.1365820/150‑1 (MQ=60)
                                                                                                                   ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                               <  SRR19973070.1365851/150‑1 (MQ=60)
                                                                                                                               CAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATA                   <  SRR19973070.2197750/150‑1 (MQ=60)
                                                                                                                               CAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATA                   >  SRR19973070.174301/1‑150 (MQ=60)
                                                                                                                                AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                  <  SRR19973070.81121/150‑1 (MQ=60)
                                                                                                                                        TGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCG          >  SRR19973070.1377906/1‑150 (MQ=60)
                                                                                                                                        TGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCG          >  SRR19973070.1658497/1‑150 (MQ=60)
                                                                                                                                           GTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAA       >  SRR19973070.674183/1‑150 (MQ=60)
                                                                                                                                           GTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAA       >  SRR19973070.674188/1‑150 (MQ=60)
                                                                                                                                            TTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAC      >  SRR19973070.2734148/1‑150 (MQ=60)
                                                                                                                                            TTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAC      >  SRR19973070.2734722/1‑150 (MQ=60)
                                                                                                                                             TTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACG     >  SRR19973070.1664035/1‑150 (MQ=60)
                                                                                                                                                CCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC  <  SRR19973070.2427754/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC  >  CP009273/1220850‑1221143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑