Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,220,996 G→T 100% R181R (CGG→AGG)  minC ← cell division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,220,9960GT96.3% 888.1 / 19.4 267R181R (CGG→AGG) minCcell division inhibitor
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base T (128/129);  total (131/136)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGC  >  CP009273/1220850‑1221141
                                                                                                                                                  |                                                                                                                                                 
 ggTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTca                                                                                                                                               >  2:949631/1‑150 (MQ=255)
   ttGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcacc                                                                                                                                             >  2:2413941/1‑150 (MQ=255)
    tGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcacca                                                                                                                                            >  2:1514312/1‑150 (MQ=255)
     gAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcaccac                                                                                                                                           >  1:2719792/1‑150 (MQ=255)
     gAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcaccac                                                                                                                                           >  2:4494620/1‑150 (MQ=255)
       aCGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACtt                                                                                                                                         <  2:4382808/150‑1 (MQ=255)
        cGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTg                                                                                                                                        >  2:2476878/1‑150 (MQ=255)
         ggTCAAAGAGTTTTCGACTAACTGCAGTCGCGCCGCTGTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCTGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGc                                                                                                                                       <  1:3417662/150‑1 (MQ=255)
             aaaGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCt                                                                                                                                   >  1:3974501/1‑150 (MQ=255)
               aGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGc                                                                                                                                 <  2:3340400/150‑1 (MQ=255)
                gCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGcc                                                                                                                                <  2:1159444/150‑1 (MQ=255)
                 cGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCa                                                                                                                               <  1:1901761/150‑1 (MQ=255)
                 cGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCa                                                                                                                               <  1:2523146/150‑1 (MQ=255)
                  gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                              >  1:1854076/1‑150 (MQ=255)
                  gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                              >  2:3809663/1‑150 (MQ=255)
                     ttCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAgcgc                                                                                                                           >  1:1817391/1‑150 (MQ=255)
                        gACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACg                                                                                                                        >  2:2543247/1‑150 (MQ=255)
                         aCTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGa                                                                                                                       >  1:2467242/1‑150 (MQ=255)
                          cTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAc                                                                                                                      >  1:1680822/1‑150 (MQ=255)
                          cTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAc                                                                                                                      >  2:2445550/1‑150 (MQ=255)
                           tAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGAcc                                                                                                                     <  2:2066173/150‑1 (MQ=255)
                            aaCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcg                                                                                                                    >  2:4739382/1‑150 (MQ=255)
                             aCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcgc                                                                                                                   <  1:4262840/150‑1 (MQ=255)
                                  aGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatc                                                                                                              >  2:4188313/1‑150 (MQ=255)
                                   gTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatca                                                                                                             >  2:2617674/1‑150 (MQ=255)
                                    tCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatcat                                                                                                            >  2:2127993/1‑150 (MQ=255)
                                    tCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGcatcat                                                                                                            <  2:4994735/150‑1 (MQ=255)
                                     cgcgCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATg                                                                                                           <  2:1279254/150‑1 (MQ=255)
                                          cgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATa                                                                                                      <  1:2336248/150‑1 (MQ=255)
                                          cgcTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATa                                                                                                      >  1:789873/1‑150 (MQ=255)
                                            cTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGa                                                                                                    >  2:4613068/1‑150 (MQ=255)
                                             tttGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAc                                                                                                   >  1:2678857/1‑150 (MQ=255)
                                               tGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCCTAGACAt                                                                                                 >  2:1699132/1‑150 (MQ=255)
                                               tGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACAt                                                                                                 >  2:3199072/1‑150 (MQ=255)
                                                gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                >  1:2810206/1‑150 (MQ=255)
                                                 ccATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGa                                                                                               <  2:3780744/150‑1 (MQ=255)
                                                  cATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGaa                                                                                              <  2:903149/150‑1 (MQ=255)
                                                  cATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGaa                                                                                              <  2:3478140/150‑1 (MQ=255)
                                                   aTAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAt                                                                                             <  2:2384767/150‑1 (MQ=255)
                                                   aTAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAt                                                                                             >  1:4424116/1‑150 (MQ=255)
                                                    tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATg                                                                                            <  1:2479806/150‑1 (MQ=255)
                                                    tAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATg                                                                                            >  1:681145/1‑150 (MQ=255)
                                                        aTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTcc                                                                                        >  1:1971043/1‑150 (MQ=255)
                                                         ttCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTccc                                                                                       <  2:826891/150‑1 (MQ=255)
                                                            tgctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATc                                                                                    >  2:1668153/1‑150 (MQ=255)
                                                             gcggggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCg                                                                                   <  1:964991/147‑1 (MQ=255)
                                                               tgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                 >  2:3078885/1‑150 (MQ=255)
                                                                 ggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                               <  2:556261/150‑1 (MQ=255)
                                                                  gATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaat                                                                              <  2:77806/150‑1 (MQ=255)
                                                                    tttGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatca                                                                            <  2:5092064/150‑1 (MQ=255)
                                                                     ttGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaa                                                                           >  2:687965/1‑150 (MQ=255)
                                                                     ttGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTGTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaatcaa                                                                           <  2:3246940/150‑1 (MQ=255)
                                                                         tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCg                                                                       >  1:1795834/1‑150 (MQ=255)
                                                                         tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCg                                                                       <  1:3377312/150‑1 (MQ=255)
                                                                         tCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCg                                                                       <  2:84377/150‑1 (MQ=255)
                                                                         gcACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCCGGTTCGTACAAGATATATGCGTTTCCCTGTCACCACTTGCCCCAGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCAACGGCAATCAATACg                                                                       >  2:1730800/2‑150 (MQ=255)
                                                                            cTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcc                                                                    >  1:5064775/1‑150 (MQ=255)
                                                                             tCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                   <  1:3816267/150‑1 (MQ=255)
                                                                             tCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                   >  1:4209386/1‑150 (MQ=255)
                                                                              cAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcccc                                                                  <  1:1417076/150‑1 (MQ=255)
                                                                               aGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCACTCAATTCGGCCCCa                                                                 >  1:1472689/1‑150 (MQ=255)
                                                                                gCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAg                                                                <  1:4899738/150‑1 (MQ=255)
                                                                                    gTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                            <  2:4179530/150‑1 (MQ=255)
                                                                                      aTTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTaa                                                          >  2:206589/1‑150 (MQ=255)
                                                                                         cACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGt                                                       >  2:4847921/1‑150 (MQ=255)
                                                                                         cACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGt                                                       >  2:2305449/1‑150 (MQ=255)
                                                                                         cACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGt                                                       <  2:1775903/150‑1 (MQ=255)
                                                                                          aCCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTg                                                      >  2:2319301/1‑150 (MQ=255)
                                                                                           ccTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTgg                                                     <  2:2589379/150‑1 (MQ=255)
                                                                                            cTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGc                                                    >  2:2554013/1‑150 (MQ=255)
                                                                                                gATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGt                                                >  2:2189640/1‑150 (MQ=255)
                                                                                                gATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGt                                                <  1:2050305/150‑1 (MQ=255)
                                                                                                 aTGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTa                                               <  2:752664/150‑1 (MQ=255)
                                                                                                  tGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                              >  1:4141938/1‑150 (MQ=255)
                                                                                                  tGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                              >  1:1135202/1‑150 (MQ=255)
                                                                                                  tGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                              <  2:3910015/150‑1 (MQ=255)
                                                                                                        ccAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCa                                        <  1:432338/150‑1 (MQ=255)
                                                                                                            ttCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATc                                    <  2:1182781/150‑1 (MQ=255)
                                                                                                             tCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATca                                   <  1:2962992/150‑1 (MQ=255)
                                                                                                              ccGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATcac                                  <  2:1623617/150‑1 (MQ=255)
                                                                                                               cGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATcaca                                 >  2:2973935/1‑150 (MQ=255)
                                                                                                                   aTCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtgt                             <  2:1357965/150‑1 (MQ=255)
                                                                                                                   aTCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtgt                             >  2:3617098/1‑150 (MQ=255)
                                                                                                                        gTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGc                        >  2:661147/1‑150 (MQ=255)
                                                                                                                          tCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCAt                      <  1:2397109/150‑1 (MQ=255)
                                                                                                                          tCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCAt                      <  1:173363/150‑1 (MQ=255)
                                                                                                                           cGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATa                     <  1:1536412/150‑1 (MQ=255)
                                                                                                                           cGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATa                     <  2:887324/150‑1 (MQ=255)
                                                                                                                             tACAAAATATGTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATaaa                   <  2:2811684/150‑1 (MQ=255)
                                                                                                                                aaaaTATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                <  2:3561462/150‑1 (MQ=255)
                                                                                                                                 aaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACg               <  1:1188866/150‑1 (MQ=255)
                                                                                                                                  aaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGc              >  1:2294786/1‑150 (MQ=255)
                                                                                                                                    tatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTg            <  1:1315597/150‑1 (MQ=255)
                                                                                                                                     atTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGa           <  1:2722153/150‑1 (MQ=255)
                                                                                                                                       ttGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAcc         <  1:511045/150‑1 (MQ=255)
                                                                                                                                        tGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCg        <  1:3751591/150‑1 (MQ=255)
                                                                                                                                         gCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCgg       <  1:3111199/150‑1 (MQ=255)
                                                                                                                                         gCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCgg       <  2:2738093/150‑1 (MQ=255)
                                                                                                                                           gTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa     >  1:1938907/1‑150 (MQ=255)
                                                                                                                                            tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAc    >  1:3652347/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGC  >  CP009273/1220850‑1221141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCG  >  CP009273/1220847‑1221145
                                                                                                                                                     |                                                                                                                                                     
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCT                                                                                                                                                       >  SRR19973077.1574885/1‑150 (MQ=60)
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATATGCGTTTCCCT                                                                                                                                                       >  SRR19973077.1574876/1‑150 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                      >  SRR19973077.3079724/1‑150 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                      >  SRR19973077.1680145/1‑150 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                      <  SRR19973077.1235020/150‑1 (MQ=60)
 AACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTG                                                                                                                                                      <  SRR19973077.367222/150‑1 (MQ=60)
   CGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTC                                                                                                                                                    >  SRR19973077.1497343/1‑150 (MQ=60)
        GAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCAC                                                                                                                                               >  SRR19973077.2724641/1‑150 (MQ=60)
        GAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCAC                                                                                                                                               >  SRR19973077.2724678/1‑150 (MQ=60)
           CGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTG                                                                                                                                            <  SRR19973077.2283809/150‑1 (MQ=60)
            GGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGC                                                                                                                                           >  SRR19973077.2829826/1‑150 (MQ=60)
            GGTCAAAGAGTTTTCGACTAACTGCAGTCGCGCCGCTGTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCTGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGC                                                                                                                                           <  SRR19973077.3423668/150‑1 (MQ=60)
              TCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCC                                                                                                                                         >  SRR19973077.1965278/1‑150 (MQ=60)
                AAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCT                                                                                                                                       >  SRR19973077.3981524/1‑150 (MQ=60)
                 AAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTG                                                                                                                                      >  SRR19973077.1791601/1‑150 (MQ=60)
                  AGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGC                                                                                                                                     >  SRR19973077.3911930/1‑150 (MQ=60)
                    CGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCA                                                                                                                                   <  SRR19973077.1905087/150‑1 (MQ=60)
                    CGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCA                                                                                                                                   <  SRR19973077.1905096/150‑1 (MQ=60)
                    CGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCA                                                                                                                                   <  SRR19973077.2527615/150‑1 (MQ=60)
                     GTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAG                                                                                                                                  >  SRR19973077.1857332/1‑150 (MQ=60)
                        TTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGC                                                                                                                               >  SRR19973077.1820580/1‑150 (MQ=60)
                        TTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGC                                                                                                                               >  SRR19973077.1820527/1‑150 (MQ=60)
                        TTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGC                                                                                                                               >  SRR19973077.1206991/1‑150 (MQ=60)
                           GACTAACTGCCGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACG                                                                                                                            >  SRR19973077.3820237/1‑150 (MQ=60)
                           GACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACG                                                                                                                            >  SRR19973077.3904648/1‑150 (MQ=60)
                           GACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACG                                                                                                                            <  SRR19973077.1938807/150‑1 (MQ=60)
                           GACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATGTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACG                                                                                                                            <  SRR19973077.297879/150‑1 (MQ=60)
                            ACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGA                                                                                                                           >  SRR19973077.2471603/1‑150 (MQ=60)
                             CTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAC                                                                                                                          >  SRR19973077.1684188/1‑150 (MQ=60)
                             CTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGAC                                                                                                                          >  SRR19973077.1683722/1‑150 (MQ=60)
                              TAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACC                                                                                                                         <  SRR19973077.2438024/150‑1 (MQ=60)
                               AACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCG                                                                                                                        >  SRR19973077.1754803/1‑150 (MQ=60)
                                ACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGC                                                                                                                       <  SRR19973077.4270322/150‑1 (MQ=60)
                                 CTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCG                                                                                                                      <  SRR19973077.2008959/150‑1 (MQ=60)
                                 CTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCG                                                                                                                      <  SRR19973077.2387988/150‑1 (MQ=60)
                                 CTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCG                                                                                                                      <  SRR19973077.2008964/150‑1 (MQ=60)
                                 CTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCG                                                                                                                      <  SRR19973077.4937173/150‑1 (MQ=60)
                                  TGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGC                                                                                                                     >  SRR19973077.1801844/1‑150 (MQ=60)
                                     AGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATC                                                                                                                  >  SRR19973077.682626/1‑150 (MQ=60)
                                     AGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATC                                                                                                                  >  SRR19973077.2398721/1‑150 (MQ=60)
                                        CGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATG                                                                                                               >  SRR19973077.252624/1‑150 (MQ=60)
                                             CGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATA                                                                                                          <  SRR19973077.2340373/150‑1 (MQ=60)
                                             CGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATA                                                                                                          >  SRR19973077.791247/1‑150 (MQ=60)
                                                TTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAC                                                                                                       >  SRR19973077.2683630/1‑150 (MQ=60)
                                                 TTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACA                                                                                                      <  SRR19973077.4309375/150‑1 (MQ=60)
                                                 TTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATAATTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACA                                                                                                      <  SRR19973077.4309348/150‑1 (MQ=60)
                                                   GCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATG                                                                                                    >  SRR19973077.2815210/1‑150 (MQ=60)
                                                   GCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATG                                                                                                    >  SRR19973077.2815206/1‑150 (MQ=60)
                                                      ATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAT                                                                                                 <  SRR19973077.401777/150‑1 (MQ=60)
                                                      ATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAT                                                                                                 >  SRR19973077.4431953/1‑150 (MQ=60)
                                                      ATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAT                                                                                                 >  SRR19973077.4431932/1‑150 (MQ=60)
                                                       TAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATG                                                                                                >  SRR19973077.682354/1‑150 (MQ=60)
                                                       TAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATG                                                                                                <  SRR19973077.2484198/150‑1 (MQ=60)
                                                        AAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGT                                                                                               >  SRR19973077.1555024/1‑150 (MQ=60)
                                                           ATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCC                                                                                            >  SRR19973077.1974488/1‑150 (MQ=60)
                                                            TTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCC                                                                                           <  SRR19973077.3684110/150‑1 (MQ=60)
                                                               TGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATC                                                                                        <  SRR19973077.3185156/150‑1 (MQ=60)
                                                                GCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCG                                                                                       >  SRR19973077.4239448/1‑150 (MQ=60)
                                                                GCGGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCG                                                                                       <  SRR19973077.966700/150‑1 (MQ=60)
                                                                    GGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAA                                                                                   <  SRR19973077.3289684/150‑1 (MQ=60)
                                                                     GATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAAT                                                                                  <  SRR19973077.2264374/150‑1 (MQ=60)
                                                                     GATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAAT                                                                                  >  SRR19973077.4261368/1‑150 (MQ=60)
                                                                            TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                           >  SRR19973077.1798964/1‑150 (MQ=60)
                                                                            TCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCG                                                                           <  SRR19973077.3383246/150‑1 (MQ=60)
                                                                               CTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCC                                                                        >  SRR19973077.5073755/1‑150 (MQ=60)
                                                                                TCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCC                                                                       >  SRR19973077.4216790/1‑150 (MQ=60)
                                                                                TCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCC                                                                       <  SRR19973077.3822970/150‑1 (MQ=60)
                                                                                 CAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCC                                                                      <  SRR19973077.1419549/150‑1 (MQ=60)
                                                                                 CAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCC                                                                      <  SRR19973077.1419203/150‑1 (MQ=60)
                                                                                  AGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCACTCAATTCGGCCCCA                                                                     >  SRR19973077.1475247/1‑150 (MQ=60)
                                                                                  AGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCA                                                                     >  SRR19973077.1475241/1‑150 (MQ=60)
                                                                                  AGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCA                                                                     <  SRR19973077.365783/150‑1 (MQ=60)
                                                                                  AGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCA                                                                     <  SRR19973077.3009891/150‑1 (MQ=60)
                                                                                   GCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAG                                                                    <  SRR19973077.4908447/150‑1 (MQ=60)
                                                                                    CCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGC                                                                   >  SRR19973077.997662/1‑150 (MQ=60)
                                                                                       GTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCT                                                                >  SRR19973077.4135875/1‑150 (MQ=60)
                                                                                        TATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTA                                                               >  SRR19973077.609282/1‑150 (MQ=60)
                                                                                        TATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTA                                                               <  SRR19973077.2660767/150‑1 (MQ=60)
                                                                                                TGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCT                                                       >  SRR19973077.1896003/1‑150 (MQ=60)
                                                                                                 GCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTT                                                      <  SRR19973077.4766006/150‑1 (MQ=60)
                                                                                                  CGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTG                                                     <  SRR19973077.1163594/150‑1 (MQ=60)
                                                                                                   GATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGT                                                    >  SRR19973077.5050358/1‑150 (MQ=60)
                                                                                                   GATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGT                                                    <  SRR19973077.2053867/150‑1 (MQ=60)
                                                                                                    ATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTA                                                   >  SRR19973077.3723672/1‑150 (MQ=60)
                                                                                                     TGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAA                                                  >  SRR19973077.1137178/1‑150 (MQ=60)
                                                                                                     TGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAA                                                  >  SRR19973077.954848/1‑150 (MQ=60)
                                                                                                     TGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAA                                                  >  SRR19973077.4149272/1‑150 (MQ=60)
                                                                                                     TGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAA                                                  >  SRR19973077.4149239/1‑150 (MQ=60)
                                                                                                         CACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAAT                                              <  SRR19973077.130099/150‑1 (MQ=60)
                                                                                                           CCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCA                                            <  SRR19973077.1889993/150‑1 (MQ=60)
                                                                                                           CCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCA                                            <  SRR19973077.433077/150‑1 (MQ=60)
                                                                                                           CCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCA                                            <  SRR19973077.2481263/150‑1 (MQ=60)
                                                                                                            CAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAG                                           >  SRR19973077.74764/1‑150 (MQ=60)
                                                                                                                TCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCA                                       <  SRR19973077.2968217/150‑1 (MQ=60)
                                                                                                                   GCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAT                                    <  SRR19973077.3417407/150‑1 (MQ=60)
                                                                                                                    CCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATT                                   <  SRR19973077.1828023/150‑1 (MQ=60)
                                                                                                                      ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                                 >  SRR19973077.1489486/1‑150 (MQ=60)
                                                                                                                      ATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGT                                 <  SRR19973077.2809523/150‑1 (MQ=60)
                                                                                                                       TCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTG                                <  SRR19973077.4816934/150‑1 (MQ=60)
                                                                                                                             TCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCAT                          <  SRR19973077.2401349/150‑1 (MQ=60)
                                                                                                                             TCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCAT                          <  SRR19973077.173693/150‑1 (MQ=60)
                                                                                                                              CGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTTGAGCATA                         <  SRR19973077.1539503/150‑1 (MQ=60)
                                                                                                                              CGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATA                         <  SRR19973077.1539092/150‑1 (MQ=60)
                                                                                                                                   AAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAC                    <  SRR19973077.2723040/150‑1 (MQ=60)
                                                                                                                                    AAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACG                   <  SRR19973077.1190935/150‑1 (MQ=60)
                                                                                                                                    AAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACG                   >  SRR19973077.2606556/1‑150 (MQ=60)
                                                                                                                                     AATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGC                  >  SRR19973077.2298833/1‑150 (MQ=60)
                                                                                                                                      ATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCT                 <  SRR19973077.2497305/150‑1 (MQ=60)
                                                                                                                                       TATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTG                <  SRR19973077.1317938/150‑1 (MQ=60)
                                                                                                                                       TATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTG                <  SRR19973077.1317906/150‑1 (MQ=60)
                                                                                                                                        ATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGA               <  SRR19973077.2727002/150‑1 (MQ=60)
                                                                                                                                        ATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGA               <  SRR19973077.2727007/150‑1 (MQ=60)
                                                                                                                                          TTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACC             <  SRR19973077.511941/150‑1 (MQ=60)
                                                                                                                                          TTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACC             <  SRR19973077.511952/150‑1 (MQ=60)
                                                                                                                                          TTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACC             <  SRR19973077.511954/150‑1 (MQ=60)
                                                                                                                                           TGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCG            <  SRR19973077.3758192/150‑1 (MQ=60)
                                                                                                                                            GCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGG           <  SRR19973077.3116712/150‑1 (MQ=60)
                                                                                                                                              GTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAA         >  SRR19973077.1942311/1‑150 (MQ=60)
                                                                                                                                              GTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAA         >  SRR19973077.2437196/1‑150 (MQ=60)
                                                                                                                                               TTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAC        >  SRR19973077.3658795/1‑150 (MQ=60)
                                                                                                                                                TTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACG       <  SRR19973077.2327868/150‑1 (MQ=60)
                                                                                                                                                 TCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAGTGTGGAGCATAAATACGCTGACCGGAACGC      >  SRR19973077.3460648/1‑150 (MQ=60)
                                                                                                                                                   CCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC    <  SRR19973077.754624/150‑1 (MQ=60)
                                                                                                                                                   CCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC    <  SRR19973077.754691/150‑1 (MQ=60)
                                                                                                                                                   CCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCAC    <  SRR19973077.755189/150‑1 (MQ=60)
                                                                                                                                                     TGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCG  <  SRR19973077.995048/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCG  >  CP009273/1220847‑1221145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑