Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,228,591 G→T 100% intergenic (‑105/+41) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,228,5910GT99.2% 895.3 / ‑1.5 257intergenic (‑105/+41)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (128/127);  total (130/127)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

ACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGC  >  CP009273/1228444‑1228737
                                                                                                                                                   |                                                                                                                                                  
aCGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTaa                                                                                                                                                  >  1:303623/1‑150 (MQ=255)
     ccccGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGc                                                                                                                                             >  1:1590942/1‑150 (MQ=255)
      cccGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCa                                                                                                                                            <  1:988623/150‑1 (MQ=255)
        cGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCaaa                                                                                                                                          <  2:3164052/150‑1 (MQ=255)
         ggCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAc                                                                                                                                         <  2:1422917/150‑1 (MQ=255)
         ggCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAc                                                                                                                                         >  1:2051664/1‑150 (MQ=255)
         ggCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAc                                                                                                                                         <  2:2172247/150‑1 (MQ=255)
         ggCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAc                                                                                                                                         <  2:553783/150‑1 (MQ=255)
           ccTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCGGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCg                                                                                                                                       <  1:4145981/150‑1 (MQ=255)
           ccTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCg                                                                                                                                       <  1:4050690/150‑1 (MQ=255)
            cTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCgg                                                                                                                                      >  2:1245609/1‑150 (MQ=255)
                 gAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAtt                                                                                                                                 <  1:2946423/150‑1 (MQ=255)
                  aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAttt                                                                                                                                <  2:207971/150‑1 (MQ=255)
                  aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAttt                                                                                                                                <  1:3773554/150‑1 (MQ=255)
                   aCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTta                                                                                                                               <  2:3279868/150‑1 (MQ=255)
                     aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTtata                                                                                                                             >  1:2237727/1‑150 (MQ=255)
                      ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTatat                                                                                                                            <  2:476674/150‑1 (MQ=255)
                         gTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGcc                                                                                                                         <  1:67695/150‑1 (MQ=255)
                          ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                        <  2:3651060/150‑1 (MQ=255)
                          ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                        <  2:725778/150‑1 (MQ=255)
                          ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                        >  2:4376307/1‑150 (MQ=255)
                           tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCg                                                                                                                       >  1:2458544/1‑150 (MQ=255)
                            cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCgg                                                                                                                      >  1:1199829/1‑150 (MQ=255)
                            cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCgg                                                                                                                      >  2:2850740/1‑150 (MQ=255)
                             aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGt                                                                                                                     <  2:1348836/150‑1 (MQ=255)
                              aaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                    >  2:2787152/1‑150 (MQ=255)
                              aaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                    >  1:4474591/1‑150 (MQ=255)
                              aaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                    >  1:2206793/1‑150 (MQ=255)
                                 aTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgt                                                                                                                 >  1:1026511/1‑150 (MQ=255)
                                  tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgtt                                                                                                                <  2:918989/150‑1 (MQ=255)
                                  tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgtt                                                                                                                >  1:3475671/1‑150 (MQ=255)
                                    gCAACATACTCAGTTCCCTGCAATCTGCAGCGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCCGGAGGCAGAAAAAATACGACGCAATTGGACACTTATCGGGCTACAAGGCAAACCGGGCATTTCTATGCCCGGTAAgttgt                                                                                                              <  2:2665462/150‑1 (MQ=255)
                                       aCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTAt                                                                                                           >  2:597142/1‑150 (MQ=255)
                                                tttCCCTGAAATATGCATTGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                  <  1:470947/150‑1 (MQ=255)
                                                    ccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTa                                                                                              >  2:1016619/1‑150 (MQ=255)
                                                        cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCa                                                                                          <  2:2879978/150‑1 (MQ=255)
                                                         aaTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAg                                                                                         <  2:238887/150‑1 (MQ=255)
                                                         aaTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAg                                                                                         >  1:1786767/1‑150 (MQ=255)
                                                          atatGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGc                                                                                        >  2:2752341/1‑150 (MQ=255)
                                                            atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc                                                                                      <  2:163855/150‑1 (MQ=255)
                                                             tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCa                                                                                     >  1:3073358/1‑150 (MQ=255)
                                                              gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                    >  1:1473939/1‑150 (MQ=255)
                                                                aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAttt                                                                                  <  1:3182527/150‑1 (MQ=255)
                                                                  agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                >  2:2096337/1‑150 (MQ=255)
                                                                  agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                >  2:2096154/1‑150 (MQ=255)
                                                                   gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                               >  2:3395708/1‑150 (MQ=255)
                                                                   gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                               >  2:3395741/1‑150 (MQ=255)
                                                                    agTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAt                                                                              <  1:4079828/150‑1 (MQ=255)
                                                                    agTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAt                                                                              <  2:3206551/150‑1 (MQ=255)
                                                                     gTGCGCAGTTTAAAACAATTCATTCGCTGAGACACCAGGTGGCAGCAAAAATAGTACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCAGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATa                                                                             <  2:1863106/150‑1 (MQ=255)
                                                                      tGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa                                                                            <  2:4449758/150‑1 (MQ=255)
                                                                        cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                          <  1:4581757/150‑1 (MQ=255)
                                                                        cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                          <  1:510408/150‑1 (MQ=255)
                                                                            gTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                      >  2:3822353/1‑150 (MQ=255)
                                                                             tttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTc                                                                     <  1:1294629/150‑1 (MQ=255)
                                                                               tAAACCAATTCATTCGATGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGAAAATTATCTGGCTAAAAGTCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCgg                                                                   >  2:1589395/1‑150 (MQ=255)
                                                                                aaaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGt                                                                  <  1:3330655/150‑1 (MQ=255)
                                                                                  aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTa                                                                >  1:2682348/1‑150 (MQ=255)
                                                                                   ccAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                               <  2:3513541/150‑1 (MQ=255)
                                                                                        tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                          <  1:3685077/150‑1 (MQ=255)
                                                                                         cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                         <  1:4384664/150‑1 (MQ=255)
                                                                                         cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                         >  2:1610214/1‑150 (MQ=255)
                                                                                          attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                        >  1:656043/1‑150 (MQ=255)
                                                                                           ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                       <  1:1335583/150‑1 (MQ=255)
                                                                                            tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                      >  2:2029162/1‑150 (MQ=255)
                                                                                            tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                      <  1:2317690/150‑1 (MQ=255)
                                                                                               cTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGt                                                   <  2:1842014/150‑1 (MQ=255)
                                                                                               cTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGt                                                   <  2:247076/150‑1 (MQ=255)
                                                                                                tGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTa                                                  <  2:1525264/150‑1 (MQ=255)
                                                                                                tGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTa                                                  <  2:41963/150‑1 (MQ=255)
                                                                                                   gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                               >  1:838558/1‑150 (MQ=255)
                                                                                                    ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                              <  1:3262965/150‑1 (MQ=255)
                                                                                                    ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                              >  2:171907/1‑150 (MQ=255)
                                                                                                     caccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                             >  2:261054/1‑150 (MQ=255)
                                                                                                      accaGGAGGCAGCAAAAATAGGACGCAATCGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCg                                            <  2:2065464/150‑1 (MQ=255)
                                                                                                       ccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa                                           >  2:1123204/1‑150 (MQ=255)
                                                                                                        caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCACGCGTAAACTCGAc                                          >  2:2981887/1‑150 (MQ=255)
                                                                                                        caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGAc                                          >  2:2206222/1‑150 (MQ=255)
                                                                                                        caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGAc                                          >  1:508721/1‑150 (MQ=255)
                                                                                                         aggaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACg                                         >  2:2402776/1‑150 (MQ=255)
                                                                                                         aggaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACg                                         >  2:4037131/1‑150 (MQ=255)
                                                                                                          ggaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGc                                        >  1:3959015/1‑150 (MQ=255)
                                                                                                               cagcaAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGc                                   >  2:254210/1‑150 (MQ=255)
                                                                                                                    aaaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                              <  1:2977315/150‑1 (MQ=255)
                                                                                                                    aaaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                              <  1:2850740/150‑1 (MQ=255)
                                                                                                                     aaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcg                             >  1:2075207/1‑150 (MQ=255)
                                                                                                                      aaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcga                            >  2:3035535/1‑150 (MQ=255)
                                                                                                                        tAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                          <  2:478815/150‑1 (MQ=255)
                                                                                                                         aGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacga                         >  1:811151/1‑150 (MQ=255)
                                                                                                                          ggACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAg                        >  2:4593953/1‑150 (MQ=255)
                                                                                                                             cGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgtgt                     <  2:2325151/150‑1 (MQ=255)
                                                                                                                              gCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTc                    <  1:681279/150‑1 (MQ=255)
                                                                                                                               cAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCa                   >  1:1937735/1‑150 (MQ=255)
                                                                                                                               cAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCa                   >  1:4269548/1‑150 (MQ=255)
                                                                                                                                 aTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAgg                 <  1:1245546/150‑1 (MQ=255)
                                                                                                                                  ttGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATGTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAATCGACGCAGAGCAAGCCGACGAGTGTCAGGa                >  2:2450964/1‑150 (MQ=255)
                                                                                                                                    ggACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACg              >  1:274890/1‑150 (MQ=255)
                                                                                                                                         aTTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgt         >  2:4388916/1‑150 (MQ=255)
                                                                                                                                           tATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgtgt       <  1:1123204/150‑1 (MQ=255)
                                                                                                                                                ggCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGc  >  1:4635999/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGC  >  CP009273/1228444‑1228737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCA  >  CP009273/1228443‑1228738
                                                                                                                                                    |                                                                                                                                                   
CACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTA                                                                                                                                                    >  SRR19973072.4337477/1‑150 (MQ=60)
 ACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAA                                                                                                                                                   >  SRR19973072.304330/1‑150 (MQ=60)
   GCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAA                                                                                                                                                 >  SRR19973072.4566736/1‑150 (MQ=60)
    CGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAG                                                                                                                                                >  SRR19973072.3107791/1‑150 (MQ=60)
      CCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGC                                                                                                                                              >  SRR19973072.1594360/1‑150 (MQ=60)
       CCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCA                                                                                                                                             <  SRR19973072.990783/150‑1 (MQ=60)
         CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                           <  SRR19973072.2377846/150‑1 (MQ=60)
         CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                           <  SRR19973072.726646/150‑1 (MQ=60)
         CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                           <  SRR19973072.2971904/150‑1 (MQ=60)
         CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                           >  SRR19973072.2220021/1‑150 (MQ=60)
         CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                           <  SRR19973072.51041/150‑1 (MQ=60)
          GGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAC                                                                                                                                          >  SRR19973072.2056129/1‑150 (MQ=60)
            CCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCGGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCG                                                                                                                                        <  SRR19973072.4154675/150‑1 (MQ=60)
            CCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCG                                                                                                                                        <  SRR19973072.4059192/150‑1 (MQ=60)
             CTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGG                                                                                                                                       >  SRR19973072.1453655/1‑150 (MQ=60)
             CTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGG                                                                                                                                       >  SRR19973072.3056013/1‑150 (MQ=60)
             CTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGG                                                                                                                                       >  SRR19973072.3056015/1‑150 (MQ=60)
             CTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCAGAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGG                                                                                                                                       >  SRR19973072.1602061/1‑150 (MQ=60)
              TTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGG                                                                                                                                      >  SRR19973072.1630848/1‑150 (MQ=60)
               TGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGC                                                                                                                                     <  SRR19973072.814664/150‑1 (MQ=60)
                  GAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATT                                                                                                                                  <  SRR19973072.2952723/150‑1 (MQ=60)
                   AACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTT                                                                                                                                 >  SRR19973072.2893763/1‑150 (MQ=60)
                   AACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTT                                                                                                                                 <  SRR19973072.3781467/150‑1 (MQ=60)
                     CATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTAT                                                                                                                               >  SRR19973072.1215090/1‑150 (MQ=60)
                     CATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTAT                                                                                                                               >  SRR19973072.3472642/1‑150 (MQ=60)
                      ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATA                                                                                                                              >  SRR19973072.2242521/1‑150 (MQ=60)
                      ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATA                                                                                                                              >  SRR19973072.2242563/1‑150 (MQ=60)
                        TGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATG                                                                                                                            <  SRR19973072.4616448/150‑1 (MQ=60)
                        TGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATG                                                                                                                            <  SRR19973072.4616115/150‑1 (MQ=60)
                         GGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGC                                                                                                                           <  SRR19973072.1286506/150‑1 (MQ=60)
                          GTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCC                                                                                                                          <  SRR19973072.67862/150‑1 (MQ=60)
                          GTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCC                                                                                                                          <  SRR19973072.67873/150‑1 (MQ=60)
                          GTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCC                                                                                                                          <  SRR19973072.132711/150‑1 (MQ=60)
                            TCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCG                                                                                                                        >  SRR19973072.2463883/1‑150 (MQ=60)
                             CAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGG                                                                                                                       >  SRR19973072.1202378/1‑150 (MQ=60)
                             CAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATGTATATGCCCGG                                                                                                                       >  SRR19973072.1202272/1‑150 (MQ=60)
                               AAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTA                                                                                                                     >  SRR19973072.2211592/1‑150 (MQ=60)
                               AAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTA                                                                                                                     >  SRR19973072.4484037/1‑150 (MQ=60)
                                  ATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGT                                                                                                                  >  SRR19973072.4091339/1‑150 (MQ=60)
                                  ATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGT                                                                                                                  >  SRR19973072.4091308/1‑150 (MQ=60)
                                  ATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGT                                                                                                                  >  SRR19973072.1028738/1‑150 (MQ=60)
                                   TCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTT                                                                                                                 >  SRR19973072.3482933/1‑150 (MQ=60)
                                        ACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTAT                                                                                                            >  SRR19973072.42083/1‑150 (MQ=60)
                                           TAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAA                                                                                                         >  SRR19973072.2729653/1‑150 (MQ=60)
                                             ATCATTTCCCTGCACTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAG                                                                                                       >  SRR19973072.4073558/1‑150 (MQ=60)
                                             ATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAG                                                                                                       <  SRR19973072.86424/150‑1 (MQ=60)
                                                 TTTCCCTGAAATATGCATTGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTT                                                                                                   <  SRR19973072.472045/150‑1 (MQ=60)
                                                          AATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAG                                                                                          >  SRR19973072.1790682/1‑150 (MQ=60)
                                                           ATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGC                                                                                         >  SRR19973072.2758339/1‑150 (MQ=60)
                                                              TGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCA                                                                                      >  SRR19973072.3079850/1‑150 (MQ=60)
                                                              TGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCA                                                                                      >  SRR19973072.1906751/1‑150 (MQ=60)
                                                              TGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCA                                                                                      >  SRR19973072.1906805/1‑150 (MQ=60)
                                                               GCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAT                                                                                     >  SRR19973072.1477594/1‑150 (MQ=60)
                                                               GCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAT                                                                                     >  SRR19973072.1477095/1‑150 (MQ=60)
                                                                 ATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTT                                                                                   >  SRR19973072.770303/1‑150 (MQ=60)
                                                                 ATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTT                                                                                   <  SRR19973072.4048331/150‑1 (MQ=60)
                                                                 ATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTT                                                                                   <  SRR19973072.3189239/150‑1 (MQ=60)
                                                                 ATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTT                                                                                   <  SRR19973072.3032694/150‑1 (MQ=60)
                                                                     AGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAT                                                                               <  SRR19973072.4088391/150‑1 (MQ=60)
                                                                     AGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAT                                                                               <  SRR19973072.3562623/150‑1 (MQ=60)
                                                                      GTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATA                                                                              >  SRR19973072.2568112/1‑150 (MQ=60)
                                                                         CGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAC                                                                           <  SRR19973072.511627/150‑1 (MQ=60)
                                                                         CGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAC                                                                           <  SRR19973072.4591390/150‑1 (MQ=60)
                                                                              TTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTC                                                                      <  SRR19973072.1297432/150‑1 (MQ=60)
                                                                                 AAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGT                                                                   <  SRR19973072.4324006/150‑1 (MQ=60)
                                                                                 AAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGT                                                                   <  SRR19973072.3337725/150‑1 (MQ=60)
                                                                                   ACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTA                                                                 >  SRR19973072.2379817/1‑150 (MQ=60)
                                                                                   ACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTA                                                                 >  SRR19973072.2688067/1‑150 (MQ=60)
                                                                                    CCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAC                                                                <  SRR19973072.2285788/150‑1 (MQ=60)
                                                                                       ATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGG                                                             <  SRR19973072.3188654/150‑1 (MQ=60)
                                                                                         TCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGG                                                           <  SRR19973072.3692846/150‑1 (MQ=60)
                                                                                         TCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGG                                                           <  SRR19973072.353972/150‑1 (MQ=60)
                                                                                         TCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGG                                                           >  SRR19973072.4330004/1‑150 (MQ=60)
                                                                                          CATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGC                                                          <  SRR19973072.3300826/150‑1 (MQ=60)
                                                                                          CATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGC                                                          <  SRR19973072.4393880/150‑1 (MQ=60)
                                                                                           ATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCA                                                         >  SRR19973072.657527/1‑150 (MQ=60)
                                                                                            TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                        <  SRR19973072.1338503/150‑1 (MQ=60)
                                                                                             TCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAG                                                       <  SRR19973072.2322646/150‑1 (MQ=60)
                                                                                                    GCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAC                                                >  SRR19973072.840363/1‑150 (MQ=60)
                                                                                                    GCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAC                                                >  SRR19973072.840777/1‑150 (MQ=60)
                                                                                                     CCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACT                                               <  SRR19973072.3269890/150‑1 (MQ=60)
                                                                                                         CAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGAC                                           >  SRR19973072.509935/1‑150 (MQ=60)
                                                                                                         CAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATAAGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTAGCATAAACCATACGGTTCCAGGGGCAAGCGTAAACTCGAC                                           >  SRR19973072.507345/1‑150 (MQ=60)
                                                                                                           GGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGC                                         >  SRR19973072.3967287/1‑150 (MQ=60)
                                                                                                               GCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAG                                     >  SRR19973072.2063330/1‑150 (MQ=60)
                                                                                                                    AAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGC                                >  SRR19973072.4454559/1‑150 (MQ=60)
                                                                                                                     AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                               <  SRR19973072.2983666/150‑1 (MQ=60)
                                                                                                                     AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                               <  SRR19973072.2983656/150‑1 (MQ=60)
                                                                                                                     AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                               <  SRR19973072.2856833/150‑1 (MQ=60)
                                                                                                                     AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                               <  SRR19973072.2856636/150‑1 (MQ=60)
                                                                                                                     AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                               <  SRR19973072.1356805/150‑1 (MQ=60)
                                                                                                                      AAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCG                              >  SRR19973072.2079583/1‑150 (MQ=60)
                                                                                                                      AAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCG                              >  SRR19973072.2079726/1‑150 (MQ=60)
                                                                                                                      AAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCG                              >  SRR19973072.2079729/1‑150 (MQ=60)
                                                                                                                       AATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGA                             >  SRR19973072.102986/1‑150 (MQ=60)
                                                                                                                          AGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGA                          >  SRR19973072.812890/1‑150 (MQ=60)
                                                                                                                          AGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGA                          <  SRR19973072.4036023/150‑1 (MQ=60)
                                                                                                                            GACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGT                        >  SRR19973072.3860014/1‑150 (MQ=60)
                                                                                                                               GCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTC                     <  SRR19973072.682790/150‑1 (MQ=60)
                                                                                                                               GCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTC                     <  SRR19973072.682775/150‑1 (MQ=60)
                                                                                                                               GCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTC                     <  SRR19973072.682739/150‑1 (MQ=60)
                                                                                                                               GCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTC                     >  SRR19973072.3826097/1‑150 (MQ=60)
                                                                                                                                CAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCA                    >  SRR19973072.4278430/1‑150 (MQ=60)
                                                                                                                                CAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCA                    >  SRR19973072.1941860/1‑150 (MQ=60)
                                                                                                                                 AATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAG                   <  SRR19973072.247122/150‑1 (MQ=60)
                                                                                                                                  ATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGG                  <  SRR19973072.651346/150‑1 (MQ=60)
                                                                                                                                  ATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGG                  <  SRR19973072.1248207/150‑1 (MQ=60)
                                                                                                                                  ATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGG                  <  SRR19973072.1248270/150‑1 (MQ=60)
                                                                                                                                  ATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGG                  >  SRR19973072.3197918/1‑150 (MQ=60)
                                                                                                                                     GGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACG               >  SRR19973072.275535/1‑150 (MQ=60)
                                                                                                                                     GGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACG               >  SRR19973072.115080/1‑150 (MQ=60)
                                                                                                                                         AATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGG           >  SRR19973072.4585771/1‑150 (MQ=60)
                                                                                                                                          ATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGT          >  SRR19973072.2004129/1‑150 (MQ=60)
                                                                                                                                           TTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTG         <  SRR19973072.3281531/150‑1 (MQ=60)
                                                                                                                                            TATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGT        <  SRR19973072.1125567/150‑1 (MQ=60)
                                                                                                                                               CGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAG     >  SRR19973072.594153/1‑150 (MQ=60)
                                                                                                                                               CGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAG     <  SRR19973072.475523/150‑1 (MQ=60)
                                                                                                                                                GGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGG    <  SRR19973072.3748180/150‑1 (MQ=60)
                                                                                                                                                 GGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGC   >  SRR19973072.4645701/1‑150 (MQ=60)
                                                                                                                                                 GGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGC   >  SRR19973072.4645698/1‑150 (MQ=60)
                                                                                                                                                  GCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCA  >  SRR19973072.3026539/1‑150 (MQ=60)
                                                                                                                                                  GCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCA  >  SRR19973072.3026435/1‑150 (MQ=60)
                                                                                                                                                  GCTAAAAGGCAAACCGCGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCAATCCACCCCATATGCATAAACAATTCGGTTACAGCGGCAAGCGTAAACTCCACGCAGAGCAAGCCGACGATTGTCAGGACTAGGGTGTAAGGCA  >  SRR19973072.3026675/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCA  >  CP009273/1228443‑1228738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑