Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,228,591 G→T 100% intergenic (‑105/+41) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,228,5910GT96.0% 613.8 / 13.7 176intergenic (‑105/+41)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (95/74);  total (98/78)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

CGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGG  >  CP009273/1228447‑1228736
                                                                                                                                                |                                                                                                                                                 
cgcCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAg                                                                                                                                              <  1:3090436/150‑1 (MQ=255)
    ccGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCaa                                                                                                                                          >  1:3042304/1‑150 (MQ=255)
     cGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCaaa                                                                                                                                         <  1:715808/150‑1 (MQ=255)
       gCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAcc                                                                                                                                       <  1:4438028/150‑1 (MQ=255)
       gCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAcc                                                                                                                                       <  1:4437994/150‑1 (MQ=255)
          ttGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCggg                                                                                                                                    >  2:1407940/1‑150 (MQ=255)
          ttGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCggg                                                                                                                                    <  2:3676859/150‑1 (MQ=255)
             tgAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAt                                                                                                                                 <  1:2016424/150‑1 (MQ=255)
               aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAttt                                                                                                                               >  1:1166550/1‑150 (MQ=255)
                aCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTta                                                                                                                              >  2:1028085/1‑150 (MQ=255)
                  aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTtata                                                                                                                            >  1:4818894/1‑150 (MQ=255)
                  aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTtata                                                                                                                            >  1:2003640/1‑150 (MQ=255)
                   ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTatat                                                                                                                           <  2:2302703/150‑1 (MQ=255)
                    tGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATg                                                                                                                          <  2:596830/150‑1 (MQ=255)
                       ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                       >  2:1676458/1‑150 (MQ=255)
                       ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                       <  2:2351924/150‑1 (MQ=255)
                       ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                       <  2:227480/150‑1 (MQ=255)
                        tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCg                                                                                                                      >  1:2797662/1‑150 (MQ=255)
                           aaaaTCGCAACCTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                   <  2:4991870/150‑1 (MQ=255)
                           aaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGtc                                                                                                                   >  2:83005/1‑149 (MQ=255)
                            aaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTaa                                                                                                                  >  1:4236270/1‑150 (MQ=255)
                            aaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTaa                                                                                                                  <  2:2329289/150‑1 (MQ=255)
                                cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                              <  2:548713/150‑1 (MQ=255)
                                 gCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgt                                                                                                             >  1:368095/1‑150 (MQ=255)
                                  cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                            <  1:4167245/150‑1 (MQ=255)
                                      aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCa                                                                                                        <  1:5002572/150‑1 (MQ=255)
                                      aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCa                                                                                                        <  2:4352478/150‑1 (MQ=255)
                                        aaTCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCaaa                                                                                                      <  2:1863940/150‑1 (MQ=255)
                                           cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTCAGTTGTTATCAAAGCg                                                                                                   >  2:4506506/1‑150 (MQ=255)
                                            aTTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                  >  1:3241079/1‑150 (MQ=255)
                                            aTTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                  >  1:3736330/1‑150 (MQ=255)
                                            aTTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                  >  2:4846017/1‑150 (MQ=255)
                                             tttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                 <  1:1985177/150‑1 (MQ=255)
                                                cccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCt                                                                                              <  2:4718631/150‑1 (MQ=255)
                                                cccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATGATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCt                                                                                              <  2:2019488/150‑1 (MQ=255)
                                                cccTGCAATATACATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCt                                                                                              <  2:944977/150‑1 (MQ=255)
                                                cccTGCAATATACATAGAGTGCGCAGTTTAAAACAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCt                                                                                              <  2:3543849/150‑1 (MQ=255)
                                                 ccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGCCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTa                                                                                             <  1:3908183/150‑1 (MQ=255)
                                                    gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                          >  2:416934/1‑150 (MQ=255)
                                                     cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCa                                                                                         <  1:5160082/150‑1 (MQ=255)
                                                     cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCa                                                                                         <  1:1194427/150‑1 (MQ=255)
                                                     cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCa                                                                                         >  1:135330/1‑150 (MQ=255)
                                                     cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAAGTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCa                                                                                         <  2:682933/150‑1 (MQ=255)
                                                      aaTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAg                                                                                        >  2:3711977/1‑150 (MQ=255)
                                                        tatGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGcc                                                                                      >  1:4305/1‑150 (MQ=255)
                                                        tatGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGcc                                                                                      <  1:2182784/150‑1 (MQ=255)
                                                          tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCa                                                                                    <  2:3807476/150‑1 (MQ=255)
                                                           gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                   <  2:2115960/150‑1 (MQ=255)
                                                             aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAttt                                                                                 <  2:2592670/150‑1 (MQ=255)
                                                               agagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                               >  2:4364162/1‑150 (MQ=255)
                                                                gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATATGACGCAATTGGACTATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCGCATTTGCa                                                                              <  1:1756349/150‑1 (MQ=255)
                                                                   tGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa                                                                           >  1:3268540/1‑150 (MQ=255)
                                                                     cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                         <  1:609861/150‑1 (MQ=255)
                                                                     cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                         <  1:609924/150‑1 (MQ=255)
                                                                       cAGTTTAAACCAATTCAGTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCa                                                                       <  2:3984263/150‑1 (MQ=255)
                                                                        aGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTCAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAt                                                                      >  2:1842102/1‑150 (MQ=255)
                                                                         gTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                     >  1:1684943/1‑150 (MQ=255)
                                                                           ttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCg                                                                   <  2:1764738/150‑1 (MQ=255)
                                                                              aaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                >  2:1014246/1‑150 (MQ=255)
                                                                                  aaTTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTGTCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAg                                                            <  2:1160901/150‑1 (MQ=255)
                                                                                  aaTTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAg                                                            <  2:2193815/150‑1 (MQ=255)
                                                                                    ttcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg                                                          <  1:841583/150‑1 (MQ=255)
                                                                                      cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                        <  1:2882054/150‑1 (MQ=255)
                                                                                      cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                        <  1:3767161/150‑1 (MQ=255)
                                                                                       attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCCTGTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                       >  2:4426130/1‑150 (MQ=255)
                                                                                       attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                       >  2:2840790/1‑150 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                      >  1:4323079/1‑150 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCac                                                      >  1:4560431/1‑149 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                      >  1:4561862/1‑150 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                      >  1:2307258/1‑150 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                      >  1:2307139/1‑150 (MQ=255)
                                                                                        ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                      >  2:2555563/1‑150 (MQ=255)
                                                                                           gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                   >  2:4062593/1‑150 (MQ=255)
                                                                                            cTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAg                                                            <  1:4727042/140‑1 (MQ=255)
                                                                                             tGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTa                                                 >  1:2537793/1‑150 (MQ=255)
                                                                                              gcgcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaa                                                >  1:1843412/1‑150 (MQ=255)
                                                                                                  caccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                            <  2:5079721/150‑1 (MQ=255)
                                                                                                   accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCg                                           >  1:4073977/1‑150 (MQ=255)
                                                                                                   accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCg                                           >  1:4074003/1‑150 (MQ=255)
                                                                                                    ccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa                                          >  2:4739083/1‑150 (MQ=255)
                                                                                                     caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGAc                                         <  2:1706402/150‑1 (MQ=255)
                                                                                                        gaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCa                                      >  2:4922805/1‑150 (MQ=255)
                                                                                                             agcaAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCa                                 >  2:1447542/1‑150 (MQ=255)
                                                                                                               caAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGTTTACAGGGGCAAGAGTATACTCGAAGCAGAGCAAg                               <  2:4305/150‑1 (MQ=255)
                                                                                                               caAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                               >  2:2486631/1‑150 (MQ=255)
                                                                                                                 aaaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                             >  2:2048461/1‑150 (MQ=255)
                                                                                                                 aaaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                             >  2:279928/1‑150 (MQ=255)
                                                                                                                 aaaaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                             >  1:2702458/1‑150 (MQ=255)
                                                                                                                   aaTAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcga                           >  2:344828/1‑150 (MQ=255)
                                                                                                                      aGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacga                        >  1:1595042/1‑150 (MQ=255)
                                                                                                                       ggACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAg                       >  2:1913116/1‑150 (MQ=255)
                                                                                                                            cAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCa                  >  2:1096870/1‑150 (MQ=255)
                                                                                                                             aaTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCTTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGGCGAGCAAGCCGACGAGTGTCAg                 >  1:4374023/1‑150 (MQ=255)
                                                                                                                                 ggACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACg             >  1:4325650/1‑150 (MQ=255)
                                                                                                                                  gACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGa            >  1:715143/1‑150 (MQ=255)
                                                                                                                                      aTTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgt        >  1:2364684/1‑150 (MQ=255)
                                                                                                                                        tATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgtgt      >  1:3101819/1‑150 (MQ=255)
                                                                                                                                          tCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTaa    <  1:153388/150‑1 (MQ=255)
                                                                                                                                          tCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTaa    <  1:3515456/150‑1 (MQ=255)
                                                                                                                                            gggCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATGTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAgg  <  2:3283310/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGG  >  CP009273/1228447‑1228736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGG  >  CP009273/1228442‑1228740
                                                                                                                                                     |                                                                                                                                                     
CCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCT                                                                                                                                                       >  SRR19973083.4890987/1‑150 (MQ=60)
CCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCT                                                                                                                                                       >  SRR19973083.3371241/1‑150 (MQ=60)
     CGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAG                                                                                                                                                  <  SRR19973083.3105238/150‑1 (MQ=60)
     CGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAG                                                                                                                                                  <  SRR19973083.2295207/150‑1 (MQ=60)
         CCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAA                                                                                                                                              >  SRR19973083.3056896/1‑150 (MQ=60)
         CCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAA                                                                                                                                              <  SRR19973083.2879889/150‑1 (MQ=60)
          CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                             <  SRR19973083.719360/150‑1 (MQ=60)
          CGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAA                                                                                                                                             <  SRR19973083.719234/150‑1 (MQ=60)
           GGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAAC                                                                                                                                            >  SRR19973083.1741297/1‑150 (MQ=60)
            GCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACC                                                                                                                                           <  SRR19973083.4457345/150‑1 (MQ=60)
            GCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACC                                                                                                                                           <  SRR19973083.4457331/150‑1 (MQ=60)
            GCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACC                                                                                                                                           <  SRR19973083.4457297/150‑1 (MQ=60)
                 GTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCA                                                                                                                                      <  SRR19973083.1320204/150‑1 (MQ=60)
                  TGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAT                                                                                                                                     <  SRR19973083.2026722/150‑1 (MQ=60)
                  TGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCAT                                                                                                                                     <  SRR19973083.2026818/150‑1 (MQ=60)
                    AACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTT                                                                                                                                   >  SRR19973083.1172350/1‑150 (MQ=60)
                       ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATA                                                                                                                                >  SRR19973083.2013871/1‑150 (MQ=60)
                       ATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATA                                                                                                                                >  SRR19973083.4839553/1‑150 (MQ=60)
                             TCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCG                                                                                                                          >  SRR19973083.2811550/1‑150 (MQ=60)
                                 AAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAA                                                                                                                      >  SRR19973083.4644223/1‑150 (MQ=60)
                                 AAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAA                                                                                                                      >  SRR19973083.4254825/1‑150 (MQ=60)
                                 AAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAA                                                                                                                      >  SRR19973083.4254817/1‑150 (MQ=60)
                                      GCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGT                                                                                                                 >  SRR19973083.369823/1‑150 (MQ=60)
                                       CAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTT                                                                                                                <  SRR19973083.4185615/150‑1 (MQ=60)
                                           ATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCA                                                                                                            <  SRR19973083.5024042/150‑1 (MQ=60)
                                              ATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAG                                                                                                         <  SRR19973083.443127/150‑1 (MQ=60)
                                                CATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCG                                                                                                       >  SRR19973083.1841899/1‑150 (MQ=60)
                                                CATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCG                                                                                                       <  SRR19973083.3196749/150‑1 (MQ=60)
                                                 ATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGT                                                                                                      >  SRR19973083.3753285/1‑150 (MQ=60)
                                                 ATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGT                                                                                                      >  SRR19973083.3256413/1‑150 (MQ=60)
                                                  TTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTT                                                                                                     <  SRR19973083.1995322/150‑1 (MQ=60)
                                                      CCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGCCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTA                                                                                                 <  SRR19973083.3925576/150‑1 (MQ=60)
                                                          CAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCA                                                                                             <  SRR19973083.5181979/150‑1 (MQ=60)
                                                          CAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCA                                                                                             <  SRR19973083.1200336/150‑1 (MQ=60)
                                                          CAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCA                                                                                             >  SRR19973083.136173/1‑150 (MQ=60)
                                                             TATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCC                                                                                          <  SRR19973083.2193705/150‑1 (MQ=60)
                                                             TATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCC                                                                                          >  SRR19973083.4335/1‑150 (MQ=60)
                                                              ATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCC                                                                                         >  SRR19973083.1950552/1‑150 (MQ=60)
                                                                 CATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATT                                                                                      >  SRR19973083.5102824/1‑150 (MQ=60)
                                                                 CATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCACGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATT                                                                                      >  SRR19973083.3684503/1‑150 (MQ=60)
                                                                     GAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATATGACGCAATTGGACTATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCGCATTTGCA                                                                                  <  SRR19973083.1764991/150‑1 (MQ=60)
                                                                     GAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCA                                                                                  >  SRR19973083.4005454/1‑150 (MQ=60)
                                                                      AGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAT                                                                                 >  SRR19973083.638946/1‑150 (MQ=60)
                                                                      AGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAT                                                                                 >  SRR19973083.831724/1‑150 (MQ=60)
                                                                        TGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAA                                                                               >  SRR19973083.3283949/1‑150 (MQ=60)
                                                                          CGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAC                                                                             <  SRR19973083.612641/150‑1 (MQ=60)
                                                                          CGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAC                                                                             <  SRR19973083.612922/150‑1 (MQ=60)
                                                                          CGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAC                                                                             <  SRR19973083.612985/150‑1 (MQ=60)
                                                                              GTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATT                                                                         >  SRR19973083.1693272/1‑150 (MQ=60)
                                                                                 TAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGG                                                                      >  SRR19973083.2016353/1‑150 (MQ=60)
                                                                                  AAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGT                                                                     >  SRR19973083.3426437/1‑150 (MQ=60)
                                                                                       cttccgatctCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAG                                                                <  SRR19973083.4747248/140‑1 (MQ=60)
                                                                                       AATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAG                                                                >  SRR19973083.3773549/1‑150 (MQ=60)
                                                                                         TTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGG                                                              <  SRR19973083.845562/150‑1 (MQ=60)
                                                                                           CATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGC                                                            <  SRR19973083.2896211/150‑1 (MQ=60)
                                                                                           CATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGC                                                            <  SRR19973083.3784200/150‑1 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCACTTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.4341843/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAC                                                          >  SRR19973083.4580177/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.4581608/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.4580764/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.4341857/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.2319080/1‑150 (MQ=60)
                                                                                             TTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAA                                                          >  SRR19973083.2318961/1‑150 (MQ=60)
                                                                                                GCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCG                                                       <  SRR19973083.2974021/150‑1 (MQ=60)
                                                                                                  TGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTA                                                     >  SRR19973083.2550668/1‑150 (MQ=60)
                                                                                                   GCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAA                                                    >  SRR19973083.1852707/1‑150 (MQ=60)
                                                                                                        ACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCG                                               >  SRR19973083.4092119/1‑150 (MQ=60)
                                                                                                        ACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCG                                               >  SRR19973083.4092077/1‑150 (MQ=60)
                                                                                                        ACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCG                                               >  SRR19973083.4092051/1‑150 (MQ=60)
                                                                                                           AGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTCAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACG                                            >  SRR19973083.3925192/1‑150 (MQ=60)
                                                                                                            GGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGC                                           >  SRR19973083.4230492/1‑150 (MQ=60)
                                                                                                               GGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGA                                        <  SRR19973083.3071424/150‑1 (MQ=60)
                                                                                                                 CAGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGC                                      >  SRR19973083.2537117/1‑150 (MQ=60)
                                                                                                                      AAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCC                                 >  SRR19973083.2715986/1‑150 (MQ=60)
                                                                                                                          TAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACG                             >  SRR19973083.3258597/1‑150 (MQ=60)
                                                                                                                           AGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGA                            >  SRR19973083.1603031/1‑150 (MQ=60)
                                                                                                                            GGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAG                           >  SRR19973083.2336089/1‑150 (MQ=60)
                                                                                                                                  AATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCTTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGGCGAGCAAGCCGACGAGTGTCAG                     >  SRR19973083.4393028/1‑150 (MQ=60)
                                                                                                                                  AATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAG                     >  SRR19973083.4392963/1‑150 (MQ=60)
                                                                                                                                  AATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAG                     >  SRR19973083.1422027/1‑150 (MQ=60)
                                                                                                                                      GGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACG                 >  SRR19973083.4344423/1‑150 (MQ=60)
                                                                                                                                       GACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGA                >  SRR19973083.718694/1‑150 (MQ=60)
                                                                                                                                           ATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGT            >  SRR19973083.2376863/1‑150 (MQ=60)
                                                                                                                                             TATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGT          >  SRR19973083.3116681/1‑150 (MQ=60)
                                                                                                                                               TCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAA        <  SRR19973083.5021138/150‑1 (MQ=60)
                                                                                                                                               TCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAA        <  SRR19973083.154334/150‑1 (MQ=60)
                                                                                                                                               TCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAA        <  SRR19973083.154294/150‑1 (MQ=60)
                                                                                                                                               TCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAA        <  SRR19973083.3531585/150‑1 (MQ=60)
                                                                                                                                                CGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAG       <  SRR19973083.2595526/150‑1 (MQ=60)
                                                                                                                                                    CTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAG   >  SRR19973083.2865700/1‑150 (MQ=60)
                                                                                                                                                    CTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAG   >  SRR19973083.2865707/1‑150 (MQ=60)
                                                                                                                                                    CTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAG   >  SRR19973083.749831/1‑150 (MQ=60)
                                                                                                                                                    CTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAG   >  SRR19973083.749914/1‑150 (MQ=60)
                                                                                                                                                     TAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGG  >  SRR19973083.4087872/1‑150 (MQ=60)
                                                                                                                                                     TAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGG  >  SRR19973083.3785638/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGG  >  CP009273/1228442‑1228740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑