Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,232,548 C→A 20.1% V50V (GTG→GTT)  ycgB ← SpoVR family stationary phase protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,232,5480CA20.1% 760.7 / 157.2 290V50V (GTG→GTT) ycgBSpoVR family stationary phase protein
Reads supporting (aligned to +/- strand):  ref base C (102/129);  new base A (31/28);  total (133/157)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 295 total aligned reads displayed.

GAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCA  >  CP009273/1232399‑1232696
                                                                                                                                                     |                                                                                                                                                    
gagTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATc                                                                                                                                                      >  2:4053039/1‑150 (MQ=255)
gagTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAAATTTTTAACGAATGACCAGTGCGGATATTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATc                                                                                                                                                      >  2:3361477/1‑150 (MQ=255)
  gTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCAc                                                                                                                                                    <  1:2092637/150‑1 (MQ=255)
   ttAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACt                                                                                                                                                   >  1:1859428/1‑150 (MQ=255)
        gACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAAt                                                                                                                                              >  1:1051415/1‑150 (MQ=255)
         aCGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATc                                                                                                                                             <  2:1967752/150‑1 (MQ=255)
           gATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTg                                                                                                                                           >  1:762384/1‑150 (MQ=255)
            aTTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTgg                                                                                                                                          >  2:3752921/1‑150 (MQ=255)
              ttCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTg                                                                                                                                        <  2:3073815/150‑1 (MQ=255)
              ttCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTg                                                                                                                                        <  2:3679187/150‑1 (MQ=255)
              ttCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTg                                                                                                                                        <  2:643772/150‑1 (MQ=255)
               tCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGc                                                                                                                                       >  1:2283474/1‑150 (MQ=255)
                   aGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGggt                                                                                                                                   >  1:561794/1‑150 (MQ=255)
                    ggCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGggta                                                                                                                                  <  1:648902/150‑1 (MQ=255)
                     gCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATATTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGggtag                                                                                                                                 >  1:4287441/1‑150 (MQ=255)
                     gCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGggtag                                                                                                                                 >  1:702113/1‑150 (MQ=255)
                      ccAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGggtagg                                                                                                                                <  1:1101857/150‑1 (MQ=255)
                      ccAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGggtagg                                                                                                                                <  2:2895971/150‑1 (MQ=255)
                         gTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTAt                                                                                                                             >  1:3451711/1‑150 (MQ=255)
                         gTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTAt                                                                                                                             <  1:1185733/150‑1 (MQ=255)
                            cTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCa                                                                                                                          >  2:2130356/1‑150 (MQ=255)
                            cTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCa                                                                                                                          >  1:4695660/1‑150 (MQ=255)
                             ttGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAg                                                                                                                         <  2:1010895/150‑1 (MQ=255)
                                  gACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGt                                                                                                                    >  1:3871240/1‑150 (MQ=255)
                                  gACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTGATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGt                                                                                                                    >  1:3553770/1‑150 (MQ=255)
                                     cGTGCTTATACAGCCGTTCAGTCTCGATAAAATTTTTACCGAATGAACAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTaga                                                                                                                 >  1:2139852/1‑150 (MQ=255)
                                       tGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGt                                                                                                               >  2:2476221/1‑150 (MQ=255)
                                       tGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGt                                                                                                               <  1:3652420/150‑1 (MQ=255)
                                          ttATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTc                                                                                                            <  1:3227509/150‑1 (MQ=255)
                                          ttATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTc                                                                                                            <  2:1566205/150‑1 (MQ=255)
                                           tataCAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCg                                                                                                           <  2:3602917/150‑1 (MQ=255)
                                           tataCAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCCGCCGGTAGAGTTTCg                                                                                                           >  2:174174/1‑150 (MQ=255)
                                           tataCAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCg                                                                                                           <  2:2721451/150‑1 (MQ=255)
                                              aCAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCa                                                                                                        <  1:4616546/150‑1 (MQ=255)
                                                       cAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTa                                                                                               >  1:4774281/1‑150 (MQ=255)
                                                       cAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTa                                                                                               <  2:49026/150‑1 (MQ=255)
                                                            tcGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATctct                                                                                          >  1:3820218/1‑150 (MQ=255)
                                                             cGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTg                                                                                         <  1:4643527/150‑1 (MQ=255)
                                                              gATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGc                                                                                        <  2:2200347/150‑1 (MQ=255)
                                                                tAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCa                                                                                      <  1:3895126/150‑1 (MQ=255)
                                                                 aaaCTTTTTACCGAATGACCAGTGCGGATAGTTAATGGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAg                                                                                     <  1:2980249/150‑1 (MQ=255)
                                                                  aaCTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGa                                                                                    >  2:2673527/1‑150 (MQ=255)
                                                                   aCTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTCGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGAt                                                                                   >  1:4354319/1‑150 (MQ=255)
                                                                   aCTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGAt                                                                                   >  1:3130930/1‑150 (MQ=255)
                                                                   aCTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGAt                                                                                   <  2:1247173/150‑1 (MQ=255)
                                                                   aCTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGAt                                                                                   <  2:1163285/150‑1 (MQ=255)
                                                                     tttttACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATaa                                                                                 >  1:1885544/1‑150 (MQ=255)
                                                                        ttACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACa                                                                              <  2:421470/150‑1 (MQ=255)
                                                                        ttACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACa                                                                              >  2:4129132/1‑150 (MQ=255)
                                                                        ttACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACa                                                                              >  1:2720223/1‑150 (MQ=255)
                                                                           ccGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATcc                                                                           >  1:1409625/1‑150 (MQ=255)
                                                                           ccGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATcc                                                                           <  1:1329227/150‑1 (MQ=255)
                                                                            cGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCca                                                                          <  1:894663/150‑1 (MQ=255)
                                                                             gAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCcag                                                                         >  2:1206669/1‑150 (MQ=255)
                                                                             gAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCcag                                                                         <  2:3267485/150‑1 (MQ=255)
                                                                              aaTGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCcagc                                                                        >  2:4444377/1‑150 (MQ=255)
                                                                              aaTGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCcagc                                                                        >  2:82026/1‑150 (MQ=255)
                                                                                tGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCcagcag                                                                      >  1:4851699/1‑150 (MQ=255)
                                                                                  aCCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGt                                                                    >  2:2635653/1‑150 (MQ=255)
                                                                                     aGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGa                                                                 >  1:1471885/1‑150 (MQ=255)
                                                                                       tGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAAc                                                               <  2:1301254/150‑1 (MQ=255)
                                                                                        gCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACg                                                              <  1:2877359/150‑1 (MQ=255)
                                                                                         cGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGt                                                             <  2:3939518/150‑1 (MQ=255)
                                                                                          ggATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTc                                                            >  2:2184793/1‑150 (MQ=255)
                                                                                           gATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTcc                                                           <  2:4588537/150‑1 (MQ=255)
                                                                                            aTAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCa                                                          <  1:669581/150‑1 (MQ=255)
                                                                                             tAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAg                                                         >  2:1355023/1‑150 (MQ=255)
                                                                                             tAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAg                                                         >  1:4808719/1‑150 (MQ=255)
                                                                                                  aaTTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCggg                                                    >  1:1014988/1‑150 (MQ=255)
                                                                                                  aaTTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCggg                                                    <  1:616658/150‑1 (MQ=255)
                                                                                                   aTTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGt                                                   <  1:3321838/150‑1 (MQ=255)
                                                                                                    ttGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTc                                                  >  1:1552827/1‑150 (MQ=255)
                                                                                                    ttGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTc                                                  >  1:173379/1‑150 (MQ=255)
                                                                                                       gCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCAt                                               <  2:906284/150‑1 (MQ=255)
                                                                                                        cATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATc                                              <  2:2292011/150‑1 (MQ=255)
                                                                                                         aTGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCg                                             <  1:2194002/150‑1 (MQ=255)
                                                                                                             tgACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCa                                         >  1:19695/2‑150 (MQ=255)
                                                                                                             cGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCa                                         <  2:875145/150‑1 (MQ=255)
                                                                                                                cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAAc                                      <  2:3336100/150‑1 (MQ=255)
                                                                                                                cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAAc                                      <  1:1010383/150‑1 (MQ=255)
                                                                                                                cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAAc                                      <  1:144190/150‑1 (MQ=255)
                                                                                                                cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAAc                                      <  2:1644768/150‑1 (MQ=255)
                                                                                                                  cTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACgt                                    >  2:4610642/1‑150 (MQ=255)
                                                                                                                    ggAGTAGGCATCCATCATCTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACgtgt                                  <  1:4581166/150‑1 (MQ=255)
                                                                                                                     gAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGtgtg                                 <  1:3048742/150‑1 (MQ=255)
                                                                                                                         aGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGgtgt                             <  2:703221/150‑1 (MQ=255)
                                                                                                                         aGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGgtgt                             <  2:701811/150‑1 (MQ=255)
                                                                                                                         aGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGgtgt                             <  2:4287441/150‑1 (MQ=255)
                                                                                                                                 atcatcTGTTCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTc                     <  1:3892601/150‑1 (MQ=255)
                                                                                                                                       tGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGACCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGaa               >  1:4424806/1‑150 (MQ=255)
                                                                                                                                          tCTGAGGTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAatcg            >  2:3702057/1‑150 (MQ=255)
                                                                                                                                            tGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAatcgat          >  2:2990968/1‑150 (MQ=255)
                                                                                                                                            tGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAatagat          >  1:3684205/1‑150 (MQ=255)
                                                                                                                                               ggTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGAtcgt       <  2:3471895/150‑1 (MQ=255)
                                                                                                                                               ggTTATAACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGAtcgt       <  2:1677415/150‑1 (MQ=255)
                                                                                                                                                  tATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGc    >  1:4110096/1‑150 (MQ=255)
                                                                                                                                                   aTCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGcc   >  2:4784958/1‑150 (MQ=255)
                                                                                                                                                   aTCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGcc   >  1:4009165/1‑150 (MQ=255)
                                                                                                                                                    tCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCa  <  2:3871371/150‑1 (MQ=255)
                                                                                                                                                    tCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCa  <  1:4280679/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCA  >  CP009273/1232399‑1232696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A