Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,235,606 G→T 33.7% intergenic (+201/+185) dadX → / ← cvrA alanine racemase, catabolic, PLP‑binding/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,235,6060GT33.7% 469.0 / 236.8 266intergenic (+201/+185)dadX/cvrAalanine racemase, catabolic, PLP‑binding/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (92/84);  new base T (36/54);  total (128/138)
Fisher's exact test for biased strand distribution p-value = 6.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 278 total aligned reads displayed.

CCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGCAGGCTCAGGCTGCGTTCAGCA  >  CP009273/1235457‑1235755
                                                                                                                                                     |                                                                                                                                                     
 catccGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGactcga                                                                                                                                                      <  2:2865834/150‑1 (MQ=255)
        cTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGAc                                                                                                                                               >  1:2720763/1‑150 (MQ=255)
          ggTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACtt                                                                                                                                             >  2:172514/1‑150 (MQ=255)
           gTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTc                                                                                                                                            <  2:4324390/150‑1 (MQ=255)
           gTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTc                                                                                                                                            <  1:31272/150‑1 (MQ=255)
               gCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGAt                                                                                                                                        <  2:2120626/150‑1 (MQ=255)
                cAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATa                                                                                                                                       >  1:1411978/1‑150 (MQ=255)
                     gTATTTGTTAAGGTGACAGTGCCATTGCCCATGATAGTGGCCATTAAAAGGATGGACACTATTTCCCCGTAAACTGAACTCACCGCACAGGCGTTATTCATAAAACGCTTACGATTCATTGTTGACTCGTACTCGACTTCATATAAATCa                                                                                                                                  <  2:2931586/150‑1 (MQ=255)
                     gTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCa                                                                                                                                  >  2:4465870/1‑150 (MQ=255)
                     gTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCa                                                                                                                                  <  2:4561570/150‑1 (MQ=255)
                      tAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCAc                                                                                                                                 <  1:3425884/150‑1 (MQ=255)
                      tAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCAc                                                                                                                                 <  2:3987727/150‑1 (MQ=255)
                       aGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACg                                                                                                                                >  1:1765893/1‑150 (MQ=255)
                        gttgttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGc                                                                                                                               >  1:319598/1‑150 (MQ=255)
                          tgttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCtt                                                                                                                             <  1:2703042/150‑1 (MQ=255)
                          tgttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCtt                                                                                                                             <  2:1074757/150‑1 (MQ=255)
                          tgttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCtt                                                                                                                             >  2:2381355/1‑150 (MQ=255)
                          tgttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCtt                                                                                                                             <  2:471355/150‑1 (MQ=255)
                            ttAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTa                                                                                                                           <  1:4381471/150‑1 (MQ=255)
                              aaTGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTAcc                                                                                                                         >  2:1428462/1‑150 (MQ=255)
                                tgtgACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCt                                                                                                                       <  2:4115655/150‑1 (MQ=255)
                                tgtgACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCt                                                                                                                       >  2:2035928/1‑150 (MQ=255)
                                 gtgACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCtt                                                                                                                      <  2:3806265/150‑1 (MQ=255)
                                      agagCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATgg                                                                                                                 >  2:570098/1‑150 (MQ=255)
                                        agCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAg                                                                                                               >  1:2644516/1‑150 (MQ=255)
                                         gCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGc                                                                                                              <  1:3863113/150‑1 (MQ=255)
                                          ccATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGACGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGcc                                                                                                             <  2:1613099/150‑1 (MQ=255)
                                           cATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCt                                                                                                            >  1:2731609/1‑150 (MQ=255)
                                            aTTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTg                                                                                                           >  1:369885/1‑150 (MQ=255)
                                                cccATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACa                                                                                                       >  2:3897482/1‑150 (MQ=255)
                                                       tAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGAttt                                                                                                >  1:3537578/1‑150 (MQ=255)
                                                       tAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGAttt                                                                                                <  1:3564609/150‑1 (MQ=255)
                                                        aGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTtg                                                                                               >  1:1132602/1‑150 (MQ=255)
                                                        aGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTtg                                                                                               >  2:4206251/1‑150 (MQ=255)
                                                        aGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACACGCGTTCTACATAAAACGCTTAAGATTCATAGTTGACTCGAAATCGACTTCAGATAAAACACACAATACCCTAGATGGAGCCAGTACATAGAttag                                                                                               >  2:3679543/1‑148 (MQ=255)
                                                         gtgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTtgt                                                                                              >  1:2622364/1‑150 (MQ=255)
                                                          tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTtgtg                                                                                             <  1:1012273/150‑1 (MQ=255)
                                                          tgtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTtgtg                                                                                             >  1:3105828/1‑150 (MQ=255)
                                                           gtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTgtgt                                                                                            <  1:1265010/150‑1 (MQ=255)
                                                           gtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTgtgt                                                                                            <  1:3164450/150‑1 (MQ=255)
                                                           gtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTgtgt                                                                                            >  1:4243399/1‑150 (MQ=255)
                                                           gtCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGAGTTgtgt                                                                                            >  1:1007675/1‑150 (MQ=255)
                                                            tCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTa                                                                                           <  2:2936422/150‑1 (MQ=255)
                                                               aTTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAAtt                                                                                        >  1:2917376/1‑150 (MQ=255)
                                                                ttAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTg                                                                                       >  2:3157898/1‑150 (MQ=255)
                                                                  aaaaGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGcc                                                                                     >  1:2886052/1‑150 (MQ=255)
                                                                      ggATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAt                                                                                 <  2:2396825/150‑1 (MQ=255)
                                                                      ggATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAt                                                                                 >  1:1589913/1‑150 (MQ=255)
                                                                      ggATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAt                                                                                 >  1:600189/1‑150 (MQ=255)
                                                                       gATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAtt                                                                                <  2:3934101/150‑1 (MQ=255)
                                                                        aTGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAttt                                                                               >  1:1635922/1‑150 (MQ=255)
                                                                         tGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGAtttt                                                                              <  1:3964251/150‑1 (MQ=255)
                                                                          ggACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTg                                                                             <  2:3577334/150‑1 (MQ=255)
                                                                           gACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGa                                                                            >  1:4469093/1‑150 (MQ=255)
                                                                             cacTATTTCCCCGGAACCTGAACTCCCCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGata                                                                          <  1:4569250/150‑1 (MQ=255)
                                                                             cacTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGata                                                                          <  2:2971722/150‑1 (MQ=255)
                                                                             cacTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGata                                                                          <  1:4262978/150‑1 (MQ=255)
                                                                              acTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGatat                                                                         <  1:2857810/150‑1 (MQ=255)
                                                                                tATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTAGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGt                                                                       >  1:3027192/1‑150 (MQ=255)
                                                                                  tttCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttc                                                                     <  1:4134031/150‑1 (MQ=255)
                                                                                   ttCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttca                                                                    <  1:3672299/150‑1 (MQ=255)
                                                                                     ccccGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGTCTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGTGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaat                                                                  <  1:4145441/150‑1 (MQ=255)
                                                                                      cccGGAACCCGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaatt                                                                 >  2:1959498/1‑150 (MQ=255)
                                                                                       ccGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaattc                                                                <  2:3540408/150‑1 (MQ=255)
                                                                                       ccGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaattc                                                                <  2:1872794/150‑1 (MQ=255)
                                                                                        cGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaattca                                                               <  2:890025/150‑1 (MQ=255)
                                                                                         ggAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaattcaa                                                              >  2:2590202/1‑150 (MQ=255)
                                                                                         ggAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGttcaattcaa                                                              >  1:2360954/1‑150 (MQ=255)
                                                                                          gAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAAc                                                             <  1:1034121/150‑1 (MQ=255)
                                                                                           aaCCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACa                                                            <  2:814472/150‑1 (MQ=255)
                                                                                           aaCCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACa                                                            <  2:2677178/150‑1 (MQ=255)
                                                                                           aaCCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACa                                                            <  1:99276/150‑1 (MQ=255)
                                                                                             ccTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATc                                                          >  2:2493996/1‑150 (MQ=255)
                                                                                               tGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCaa                                                        >  1:2952853/1‑150 (MQ=255)
                                                                                                gAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCaaa                                                       <  2:3464246/150‑1 (MQ=255)
                                                                                                 aaCTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAAt                                                      >  2:2585521/1‑150 (MQ=255)
                                                                                                  aCTCACCACACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATg                                                     <  1:1923158/150‑1 (MQ=255)
                                                                                                    tCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGaa                                                   >  1:3296157/1‑150 (MQ=255)
                                                                                                     cACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAg                                                  <  1:1895921/150‑1 (MQ=255)
                                                                                                        cGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGtt                                               <  2:3215107/150‑1 (MQ=255)
                                                                                                            caGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAAt                                           <  1:157570/150‑1 (MQ=255)
                                                                                                             aGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAAtt                                          <  2:3281589/150‑1 (MQ=255)
                                                                                                             aGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAAtt                                          >  2:19221/1‑150 (MQ=255)
                                                                                                                 gTTCTACATAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATgg                                      <  1:3610044/150‑1 (MQ=255)
                                                                                                                      aCATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGgacgac                                 >  1:1831025/1‑150 (MQ=255)
                                                                                                                         tAAAACGCTTACGCTTCATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGacaaa                              <  2:3129725/150‑1 (MQ=255)
                                                                                                                               gcttacgcttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTg                        >  1:854718/1‑150 (MQ=255)
                                                                                                                               gcttacgcttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTg                        <  2:131311/150‑1 (MQ=255)
                                                                                                                                   acgcttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcagg                    <  2:3047152/150‑1 (MQ=255)
                                                                                                                                     gcttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggct                  >  2:2223409/1‑150 (MQ=255)
                                                                                                                                      cttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctc                 <  2:3347810/150‑1 (MQ=255)
                                                                                                                                       ttCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctca                >  1:915354/1‑150 (MQ=255)
                                                                                                                                       ttCATTGTTGACACGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACAAAGATTAGTCTAATTGCCTGATATTGATATGATCAATTCAACATAAAATGAAGGTTAAATTATGGACGACAAACAATTTaatgctca                >  1:915411/1‑150 (MQ=255)
                                                                                                                                        tCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctcag               <  1:2119754/150‑1 (MQ=255)
                                                                                                                                         cATTGTTGACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctcagg              >  1:639888/1‑150 (MQ=255)
                                                                                                                                         cATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctcagg              <  2:3762652/150‑1 (MQ=255)
                                                                                                                                           ttgttgACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGcaggctcaggct            <  1:3762912/150‑1 (MQ=255)
                                                                                                                                              ttgACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGCAGGCTCAGGCTGCg         <  1:4024082/150‑1 (MQ=255)
                                                                                                                                                gACTCTAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGCAGGCTCAGGCTGCGtt       <  2:4084336/150‑1 (MQ=255)
                                                                                                                                                gACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGCAGGCTCAGGCTGCGtt       <  2:1281034/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATCCGGCTGGTCAGCAACTGTAGTTGTTAATGTGACAGAGCCATTGCCCATGATAGTGTCCATTAAAAGGATGGACACTATTTCCCCGGAACCTGAACTCACCGCACAGGCGTTCTACATAAAACGCTTACGCTTCATTGTTGACTCGAACTCGACTTCAGATAAATCACGCTTTACCCTTGATGGAGCCTGTACATAGATTTGTGTAATTGCCTGATTTTGATATGTTCAATTCAACATCAAATGAAGGTTAAATTATGGACGACAAACAATTGCAGGCTCAGGCTGCGTTCAGCA  >  CP009273/1235457‑1235755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A