Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,372,318 G→T 14.7% R347R (CGG→AGG)  yhcM ← putative AFG1‑like family P‑loop ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,372,3180GT14.7% 789.5 / 77.7 238R347R (CGG→AGG) yhcMputative AFG1‑like family P‑loop ATPase
Reads supporting (aligned to +/- strand):  ref base G (106/97);  new base T (17/18);  total (123/115)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 244 total aligned reads displayed.

GCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372169‑3372467
                                                                                                                                                     |                                                                                                                                                     
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATc                                                                                                                                                    >  2:2474286/1‑150 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATc                                                                                                                                                    >  1:2252474/1‑150 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                                                                                                    <  2:1036324/150‑1 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                                                                                                    <  1:4465125/150‑1 (MQ=255)
    gATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCg                                                                                                                                                   >  1:4238908/1‑150 (MQ=255)
       tataGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGccc                                                                                                                                                >  1:3028447/1‑150 (MQ=255)
       tataGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGccc                                                                                                                                                <  1:3727918/150‑1 (MQ=255)
             gaAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATaa                                                                                                                                          <  1:3262301/150‑1 (MQ=255)
               aGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAAt                                                                                                                                        <  2:1516943/150‑1 (MQ=255)
               aGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAt                                                                                                                                        >  2:2123257/1‑150 (MQ=255)
                gTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAAtt                                                                                                                                       >  1:515612/1‑150 (MQ=255)
                 tCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAttt                                                                                                                                      <  1:1322592/150‑1 (MQ=255)
                 tCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAttt                                                                                                                                      <  2:1456704/150‑1 (MQ=255)
                     ggTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATa                                                                                                                                  <  1:3885285/150‑1 (MQ=255)
                         aaaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGc                                                                                                                              >  2:1017130/1‑150 (MQ=255)
                          aataTCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCAAAATGCTCGCTCTTCAGATACTCTTCGCTTTGCATCTATTGCAGACGTTTCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGACCTGATAAATTTCATACAGCg                                                                                                                             <  2:2614937/147‑1 (MQ=255)
                          aaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCg                                                                                                                             <  2:1645843/150‑1 (MQ=255)
                           aGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCgg                                                                                                                            <  1:2407860/150‑1 (MQ=255)
                            gATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGc                                                                                                                           <  1:1066758/150‑1 (MQ=255)
                            gATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGc                                                                                                                           <  2:842597/150‑1 (MQ=255)
                              tCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCAc                                                                                                                         >  1:1641125/1‑150 (MQ=255)
                               cGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACt                                                                                                                        <  1:4106022/150‑1 (MQ=255)
                                gACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACtt                                                                                                                       >  1:874283/1‑150 (MQ=255)
                                gACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACtt                                                                                                                       >  2:2941189/1‑150 (MQ=255)
                                  ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCt                                                                                                                     <  2:635663/150‑1 (MQ=255)
                                  ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCt                                                                                                                     <  2:2145442/150‑1 (MQ=255)
                                  ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCt                                                                                                                     <  2:4479720/150‑1 (MQ=255)
                                   gccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTg                                                                                                                    >  1:4401742/2‑150 (MQ=255)
                                   cccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTg                                                                                                                    <  1:3538488/150‑1 (MQ=255)
                                    ccTTTTTGTGATTTGTGACAGGTTTTAACCCGCNAAATGCTCGNGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGc                                                                                                                   <  1:2532357/150‑1 (MQ=255)
                                       ttttGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACt                                                                                                                <  1:2311510/150‑1 (MQ=255)
                                          tgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcac                                                                                                             >  1:255430/1‑150 (MQ=255)
                                           gtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacc                                                                                                            >  2:3677532/1‑150 (MQ=255)
                                            tgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacca                                                                                                           >  1:4316290/1‑150 (MQ=255)
                                            tgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacca                                                                                                           >  1:1407013/1‑150 (MQ=255)
                                            tgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacca                                                                                                           <  1:2637218/150‑1 (MQ=255)
                                              atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACt                                                                                                         >  2:664297/1‑150 (MQ=255)
                                               tttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTa                                                                                                        >  1:2026493/1‑150 (MQ=255)
                                               tttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTa                                                                                                        >  1:2131251/1‑150 (MQ=255)
                                               tttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTa                                                                                                        <  2:4026061/150‑1 (MQ=255)
                                                ttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTaa                                                                                                       <  2:941557/150‑1 (MQ=255)
                                                   tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAttt                                                                                                    >  2:4147893/1‑150 (MQ=255)
                                                      cAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGAc                                                                                                 >  2:4089997/1‑150 (MQ=255)
                                                       aGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACa                                                                                                >  1:2509159/1‑150 (MQ=255)
                                                         gTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATg                                                                                              <  2:373969/150‑1 (MQ=255)
                                                             tAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc                                                                                          >  2:2905288/1‑150 (MQ=255)
                                                                cccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCg                                                                                       <  2:3210573/150‑1 (MQ=255)
                                                                cccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCg                                                                                       >  2:2548653/1‑150 (MQ=255)
                                                                cccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCg                                                                                       >  2:2248838/1‑150 (MQ=255)
                                                                 ccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGt                                                                                      <  1:334459/150‑1 (MQ=255)
                                                                    ccAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaa                                                                                   >  1:2928505/1‑150 (MQ=255)
                                                                    ccAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaa                                                                                   >  2:4422159/1‑150 (MQ=255)
                                                                        aTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAATCTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTc                                                                               >  1:3910621/1‑150 (MQ=255)
                                                                        aTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTc                                                                               >  1:4170438/1‑150 (MQ=255)
                                                                               cgcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATccacc                                                                        >  2:2372681/1‑150 (MQ=255)
                                                                               cgcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATccacc                                                                        <  1:504639/150‑1 (MQ=255)
                                                                                gcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATccacca                                                                       >  2:467074/1‑150 (MQ=255)
                                                                                gcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATccacca                                                                       <  1:263366/150‑1 (MQ=255)
                                                                                  ttCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgc                                                                     >  2:4621510/1‑150 (MQ=255)
                                                                                  ttCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgc                                                                     >  1:3693941/1‑150 (MQ=255)
                                                                                     aGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCa                                                                  >  2:2070716/1‑150 (MQ=255)
                                                                                       aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                <  2:4395115/150‑1 (MQ=255)
                                                                                       aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                >  1:3447582/1‑150 (MQ=255)
                                                                                       aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                >  2:3514558/1‑150 (MQ=255)
                                                                                        tACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATa                                                               >  1:2827658/1‑150 (MQ=255)
                                                                                           tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAg                                                            <  2:757018/150‑1 (MQ=255)
                                                                                           tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAg                                                            >  1:4160006/1‑150 (MQ=255)
                                                                                           tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAg                                                            >  1:1765553/1‑150 (MQ=255)
                                                                                           tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAATTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATaaat                                                            >  2:912423/1‑149 (MQ=255)
                                                                                             ttCGCTTTGCATCTCTTGCAGACGTGACAGGCAACACTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAgcg                                                          <  2:2993082/150‑1 (MQ=255)
                                                                                               cGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgcc                                                        >  2:1170666/1‑150 (MQ=255)
                                                                                                gCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccg                                                       <  2:2224426/150‑1 (MQ=255)
                                                                                                     gCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCtt                                                  <  2:802920/150‑1 (MQ=255)
                                                                                                       aTCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCg                                                >  2:3341878/1‑150 (MQ=255)
                                                                                                        tctcTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGc                                               >  1:2054554/1‑150 (MQ=255)
                                                                                                         ctctTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGct                                              >  1:4576491/1‑150 (MQ=255)
                                                                                                           ctTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGctct                                            <  2:813254/150‑1 (MQ=255)
                                                                                                               cAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCAt                                        <  1:3380620/150‑1 (MQ=255)
                                                                                                                 gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCa                                      >  1:3070066/1‑150 (MQ=255)
                                                                                                                   cGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAAc                                    >  2:3187050/1‑150 (MQ=255)
                                                                                                                    gTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCAGACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAAcc                                   <  2:3459786/150‑1 (MQ=255)
                                                                                                                     tGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACcg                                  <  1:1017130/150‑1 (MQ=255)
                                                                                                                      gACAGGCAACGCTGGAACTCAAACTAAAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTTACATGGCGATCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACcgc                                 >  2:2386801/1‑150 (MQ=255)
                                                                                                                             aaCGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATaa                          >  2:3392743/1‑150 (MQ=255)
                                                                                                                             aaCGCTGGAACTCAAACTTCAGCCGATCGACCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATaa                          <  2:1883481/150‑1 (MQ=255)
                                                                                                                               cGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACt                        >  2:4057280/1‑150 (MQ=255)
                                                                                                                               cGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACt                        <  1:1103694/150‑1 (MQ=255)
                                                                                                                                  tGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGt                     <  1:76775/150‑1 (MQ=255)
                                                                                                                                   ggAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTa                    <  1:1929383/150‑1 (MQ=255)
                                                                                                                                       cTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATc                >  2:2858154/1‑150 (MQ=255)
                                                                                                                                        tCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCa               <  2:4152393/150‑1 (MQ=255)
                                                                                                                                        tCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCa               >  2:3293687/1‑150 (MQ=255)
                                                                                                                                        tCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCa               >  2:2754062/1‑150 (MQ=255)
                                                                                                                                         cAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCaa              <  1:4536427/150‑1 (MQ=255)
                                                                                                                                           aaCTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaac            <  2:1313142/150‑1 (MQ=255)
                                                                                                                                            aCTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaaca           <  2:1811645/150‑1 (MQ=255)
                                                                                                                                                cAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACAt       <  2:4366045/150‑1 (MQ=255)
                                                                                                                                                    cGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGAcc   <  1:4439413/150‑1 (MQ=255)
                                                                                                                                                     gATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCg  <  2:705038/150‑1 (MQ=255)
                                                                                                                                                     gATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCg  <  2:2596703/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372169‑3372467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A