Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,372,318 G→T 42.9% R347R (CGG→AGG)  yhcM ← putative AFG1‑like family P‑loop ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,372,3180GT42.9% 214.8 / 265.2 214R347R (CGG→AGG) yhcMputative AFG1‑like family P‑loop ATPase
Reads supporting (aligned to +/- strand):  ref base G (59/63);  new base T (51/41);  total (110/104)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372169‑3372467
                                                                                                                                                     |                                                                                                                                                     
gCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCg                                                                                                                                                       <  1:71282/150‑1 (MQ=255)
 cAGGATTATAGAGAAGTCGGGGTCAAATATCGACCCCTTTTTGTGATTTGTGACAGTTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGATTTGCATCTCTGGCAGACGTGACAGACAACGCAGGAACTCAAACTTCAGCCGa                                                                                                                                                      >  2:79306/1‑150 (MQ=255)
  aGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGAt                                                                                                                                                     <  1:3783333/150‑1 (MQ=255)
  aGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGAt                                                                                                                                                     >  2:3358378/1‑150 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATc                                                                                                                                                    <  1:3570650/150‑1 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                                                                                                    <  1:3879739/150‑1 (MQ=255)
   ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                                                                                                    <  1:2562104/150‑1 (MQ=255)
    gATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCg                                                                                                                                                   <  1:2006121/150‑1 (MQ=255)
      ttATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGcc                                                                                                                                                 <  2:2415450/150‑1 (MQ=255)
         taGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTg                                                                                                                                              <  1:1220908/150‑1 (MQ=255)
            agaAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATa                                                                                                                                           <  1:3007430/150‑1 (MQ=255)
                  cGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTc                                                                                                                                     <  1:897542/150‑1 (MQ=255)
                  cGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTc                                                                                                                                     >  2:833404/1‑150 (MQ=255)
                    gggTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCAt                                                                                                                                   <  1:308503/150‑1 (MQ=255)
                      gTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATAc                                                                                                                                 >  2:360276/1‑150 (MQ=255)
                         aaaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGc                                                                                                                              >  2:2159513/1‑150 (MQ=255)
                         aaaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGc                                                                                                                              >  2:2709860/1‑150 (MQ=255)
                         aaaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACGTCAGCCGATCGCCCTGATAAATTTCATACAGc                                                                                                                              >  2:384714/1‑150 (MQ=255)
                               cGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACt                                                                                                                        >  2:3906674/1‑150 (MQ=255)
                               cGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACt                                                                                                                        >  2:281009/1‑150 (MQ=255)
                                gACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACtt                                                                                                                       >  1:1499180/1‑150 (MQ=255)
                                  ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCt                                                                                                                     <  1:1092272/150‑1 (MQ=255)
                                   cccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTg                                                                                                                    <  1:1515823/150‑1 (MQ=255)
                                      tttttGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCAc                                                                                                                 <  2:3943813/150‑1 (MQ=255)
                                        tttgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTc                                                                                                               >  2:1609984/1‑150 (MQ=255)
                                           gtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacc                                                                                                            >  1:3045778/1‑150 (MQ=255)
                                            tgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacca                                                                                                           >  2:4023238/1‑150 (MQ=255)
                                            tgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacca                                                                                                           >  1:3363519/1‑150 (MQ=255)
                                             gatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcaccac                                                                                                          <  2:1530575/150‑1 (MQ=255)
                                              atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACt                                                                                                         <  2:3687129/150‑1 (MQ=255)
                                              atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACt                                                                                                         >  2:3130145/1‑150 (MQ=255)
                                                 tgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAt                                                                                                      <  1:325242/150‑1 (MQ=255)
                                                 tgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAt                                                                                                      <  2:333467/150‑1 (MQ=255)
                                                  gtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAtt                                                                                                     >  1:2137520/1‑150 (MQ=255)
                                                   tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAttt                                                                                                    >  1:1726242/1‑150 (MQ=255)
                                                   tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAttt                                                                                                    >  1:3876419/1‑150 (MQ=255)
                                                       aGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACa                                                                                                <  2:2621796/150‑1 (MQ=255)
                                                             tAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc                                                                                          >  1:1091977/1‑150 (MQ=255)
                                                              aaCCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCt                                                                                         >  1:1708318/1‑150 (MQ=255)
                                                               aCCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTc                                                                                        <  2:1747979/150‑1 (MQ=255)
                                                               aCCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTc                                                                                        <  2:2331019/150‑1 (MQ=255)
                                                                  cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTa                                                                                     >  1:820437/1‑150 (MQ=255)
                                                                  cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTa                                                                                     >  1:4144896/1‑150 (MQ=255)
                                                                  cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTa                                                                                     <  1:534270/150‑1 (MQ=255)
                                                                   gCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaa                                                                                    >  1:2717205/1‑150 (MQ=255)
                                                                       aaTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACt                                                                                <  2:1688894/150‑1 (MQ=255)
                                                                        aTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTc                                                                               <  1:56607/150‑1 (MQ=255)
                                                                         tGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCa                                                                              >  1:103105/1‑150 (MQ=255)
                                                                          gCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACCGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCAt                                                                             >  2:2967700/1‑150 (MQ=255)
                                                                          gCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCAt                                                                             >  1:3689690/1‑150 (MQ=255)
                                                                           cTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATc                                                                            >  2:419998/1‑150 (MQ=255)
                                                                            tCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATcc                                                                           <  1:3649764/150‑1 (MQ=255)
                                                                                 cTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAg                                                                      >  1:1586764/1‑150 (MQ=255)
                                                                                  ttCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgc                                                                     <  1:4031514/150‑1 (MQ=255)
                                                                                  ttCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgc                                                                     <  1:2326294/150‑1 (MQ=255)
                                                                                   tCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgcg                                                                    <  1:1947413/150‑1 (MQ=255)
                                                                                   tCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgcg                                                                    <  1:2803347/150‑1 (MQ=255)
                                                                                       aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                >  1:3424581/1‑150 (MQ=255)
                                                                                          ctctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATaaa                                                             >  2:146667/1‑150 (MQ=255)
                                                                                           tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAg                                                            >  1:2878801/1‑150 (MQ=255)
                                                                                              tCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAgcgc                                                         >  1:3802994/1‑150 (MQ=255)
                                                                                               cGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgcc                                                        <  2:2031993/150‑1 (MQ=255)
                                                                                                gCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccg                                                       >  1:2723619/1‑150 (MQ=255)
                                                                                                gCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccg                                                       >  2:1975269/1‑150 (MQ=255)
                                                                                                gCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccg                                                       <  2:199645/150‑1 (MQ=255)
                                                                                                gCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccg                                                       >  2:3396145/1‑150 (MQ=255)
                                                                                                 cTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgccgc                                                      <  1:2589055/150‑1 (MQ=255)
                                                                                                    tGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCt                                                   >  1:4082429/1‑150 (MQ=255)
                                                                                                    tGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCt                                                   <  1:3841289/150‑1 (MQ=255)
                                                                                                    tGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCt                                                   >  1:701501/1‑150 (MQ=255)
                                                                                                     gCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCtt                                                  >  2:468350/1‑150 (MQ=255)
                                                                                                      cATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTc                                                 <  1:1107023/150‑1 (MQ=255)
                                                                                                       aTCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCg                                                <  1:449329/150‑1 (MQ=255)
                                                                                                       aTCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCg                                                <  1:2168473/150‑1 (MQ=255)
                                                                                                       aTCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCg                                                >  2:55829/1‑150 (MQ=255)
                                                                                                            ttGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCtctc                                           <  1:3439804/150‑1 (MQ=255)
                                                                                                               cAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCAt                                        >  2:1178986/1‑150 (MQ=255)
                                                                                                                aGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATc                                       >  1:1270391/1‑150 (MQ=255)
                                                                                                                aGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATc                                       <  2:795489/150‑1 (MQ=255)
                                                                                                                 gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCa                                      >  1:1980958/1‑150 (MQ=255)
                                                                                                                 gACGTGACAGGCAACGCTGGAACTCAAACATCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCa                                      <  2:2591834/150‑1 (MQ=255)
                                                                                                                     tGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACcg                                  >  2:3260903/1‑150 (MQ=255)
                                                                                                                       aCAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACcgcg                                <  2:2299763/150‑1 (MQ=255)
                                                                                                                             aaCGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATaa                          <  2:1426791/150‑1 (MQ=255)
                                                                                                                               cGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACt                        >  2:4017723/1‑150 (MQ=255)
                                                                                                                                gCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTg                       <  2:939520/150‑1 (MQ=255)
                                                                                                                                  tGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGt                     >  2:2911630/1‑150 (MQ=255)
                                                                                                                                  tGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGt                     >  2:1779697/1‑150 (MQ=255)
                                                                                                                                  tGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGt                     >  1:3347998/1‑150 (MQ=255)
                                                                                                                                   ggAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTa                    <  1:1059338/150‑1 (MQ=255)
                                                                                                                                   ggAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTa                    >  2:2463603/1‑150 (MQ=255)
                                                                                                                                   ggAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTa                    >  1:693811/1‑150 (MQ=255)
                                                                                                                                    gAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTAc                   <  1:2262557/150‑1 (MQ=255)
                                                                                                                                            aCTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCTCTCGTAAAACTCATCCACCAGCGCAATTAAGCGCCGCGCTTCGCTCTCAATCAACCGCGTCATAACTGGTACATCAaaca           <  2:2371009/150‑1 (MQ=255)
                                                                                                                                             cTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaacaa          <  1:2418153/150‑1 (MQ=255)
                                                                                                                                              ttCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaacaac         <  2:3098634/150‑1 (MQ=255)
                                                                                                                                              ttCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaacaac         <  2:406529/150‑1 (MQ=255)
                                                                                                                                              ttCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaacaac         >  1:481286/1‑150 (MQ=255)
                                                                                                                                               tCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAacaaca        >  2:3575326/1‑150 (MQ=255)
                                                                                                                                                     gATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCg  >  1:3522441/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372169‑3372467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A