Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,372,318 G→T 41.3% R347R (CGG→AGG)  yhcM ← putative AFG1‑like family P‑loop ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,372,3180GT41.3% 328.1 / inf 285R347R (CGG→AGG) yhcMputative AFG1‑like family P‑loop ATPase
Reads supporting (aligned to +/- strand):  ref base G (79/88);  new base T (59/59);  total (138/147)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

CAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372170‑3372467
                                                                                                                                                    |                                                                                                                                                     
  ggATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                                                                                                    >  2:3653104/1‑150 (MQ=255)
     ttATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGcc                                                                                                                                                 >  1:1173865/1‑150 (MQ=255)
     ttATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGcc                                                                                                                                                 >  1:1726822/1‑150 (MQ=255)
      tataGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGccc                                                                                                                                                <  2:175457/150‑1 (MQ=255)
      tataGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGccc                                                                                                                                                >  1:412568/1‑150 (MQ=255)
             aaGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATaaa                                                                                                                                         <  1:1917577/150‑1 (MQ=255)
               gTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCACCGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAtt                                                                                                                                       >  1:2919151/1‑150 (MQ=255)
               gTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAAtt                                                                                                                                       >  2:3502601/1‑150 (MQ=255)
                tCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAAttt                                                                                                                                      <  1:1440012/150‑1 (MQ=255)
                tCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAttt                                                                                                                                      >  2:2467496/1‑150 (MQ=255)
                      tCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACa                                                                                                                                >  1:1081528/1‑150 (MQ=255)
                        aaaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGc                                                                                                                              >  2:1765459/1‑150 (MQ=255)
                          aGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCgg                                                                                                                            >  2:501459/1‑150 (MQ=255)
                           gATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGc                                                                                                                           >  2:3330775/1‑150 (MQ=255)
                            aTCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCa                                                                                                                          <  1:2494238/150‑1 (MQ=255)
                            aTCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCa                                                                                                                          <  1:2680667/150‑1 (MQ=255)
                            aTCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCa                                                                                                                          <  1:1760699/150‑1 (MQ=255)
                                  cccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTg                                                                                                                    <  2:1930956/150‑1 (MQ=255)
                                    cTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCa                                                                                                                  <  2:1974078/150‑1 (MQ=255)
                                      ttttGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACt                                                                                                                <  2:3178199/150‑1 (MQ=255)
                                        ttgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTca                                                                                                              <  2:2836699/150‑1 (MQ=255)
                                        ttgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTca                                                                                                              >  2:2329873/1‑150 (MQ=255)
                                             atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACt                                                                                                         <  2:3924642/150‑1 (MQ=255)
                                             atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACt                                                                                                         <  1:4211265/150‑1 (MQ=255)
                                                tgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAt                                                                                                      <  2:1610222/150‑1 (MQ=255)
                                                tgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAt                                                                                                      <  2:2620948/150‑1 (MQ=255)
                                                  tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAttt                                                                                                    >  1:2649434/1‑150 (MQ=255)
                                                   gaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTg                                                                                                   <  1:3330874/150‑1 (MQ=255)
                                                   gaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTg                                                                                                   <  2:3538809/150‑1 (MQ=255)
                                                    aCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGa                                                                                                  >  2:348134/1‑150 (MQ=255)
                                                        gTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATg                                                                                              <  2:1367953/150‑1 (MQ=255)
                                                          tttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgc                                                                                            <  1:685470/150‑1 (MQ=255)
                                                           ttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcg                                                                                           <  2:3255227/150‑1 (MQ=255)
                                                           ttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGATTTTCATATCTTGCAGACGTGAAAGGAAAAGCTTGAACTCAAACTTCAGCATATAGACCTGATAAATTACATACAGAGGCACTTCTTAAATCACCAATAATTTGAAATGtcg                                                                                           >  2:39476/1‑150 (MQ=255)
                                                              aCCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTc                                                                                        <  1:3589281/150‑1 (MQ=255)
                                                                 cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTa                                                                                     <  1:1351018/150‑1 (MQ=255)
                                                                  gCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaa                                                                                    >  1:4628166/1‑150 (MQ=255)
                                                                    cAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaaa                                                                                  >  2:89342/1‑150 (MQ=255)
                                                                    cAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaaa                                                                                  >  2:3404110/1‑150 (MQ=255)
                                                                    cAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaaa                                                                                  <  1:3685568/150‑1 (MQ=255)
                                                                    cAAATGCTCGCGCGTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaaaa                                                                                  <  2:140842/150‑1 (MQ=255)
                                                                     aaaTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAAc                                                                                 <  2:643874/150‑1 (MQ=255)
                                                                     aaaTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAAc                                                                                 >  1:1954293/1‑150 (MQ=255)
                                                                        tGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCa                                                                              <  2:447111/150‑1 (MQ=255)
                                                                          cTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATc                                                                            >  2:244319/1‑150 (MQ=255)
                                                                          cTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATc                                                                            >  1:3167994/1‑150 (MQ=255)
                                                                           tCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATcc                                                                           <  2:2210126/150‑1 (MQ=255)
                                                                             gcgcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATccac                                                                         <  2:2528906/150‑1 (MQ=255)
                                                                                  tCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgcg                                                                    >  1:1816917/1‑150 (MQ=255)
                                                                                   cAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGGCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCCCCAgcgc                                                                   >  1:2548607/1‑150 (MQ=255)
                                                                                   cAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgcgc                                                                   >  2:2165849/1‑150 (MQ=255)
                                                                                   cAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAgcgc                                                                   <  2:3127237/150‑1 (MQ=255)
                                                                                    aGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCa                                                                  >  1:4398124/1‑150 (MQ=255)
                                                                                      aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                >  1:2368562/1‑150 (MQ=255)
                                                                                      aTACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAAt                                                                <  2:2948293/150‑1 (MQ=255)
                                                                                        aCTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATaa                                                              >  1:3869071/1‑150 (MQ=255)
                                                                                        aCTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATaa                                                              >  2:3712781/1‑150 (MQ=255)
                                                                                        aCTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTAATCCACCAGCGCAATaa                                                              >  2:1501424/1‑150 (MQ=255)
                                                                                          tctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAg                                                            <  2:1554858/150‑1 (MQ=255)
                                                                                           ctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAgc                                                           <  1:509010/150‑1 (MQ=255)
                                                                                           ctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACGTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAgc                                                           >  2:1811309/1‑150 (MQ=255)
                                                                                              cGCTTTGCATCTCTTACAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGcgcc                                                        <  2:529139/150‑1 (MQ=255)
                                                                                                 tttGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCcgcg                                                     >  1:3641082/1‑150 (MQ=255)
                                                                                                   tGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCt                                                   <  1:2172956/150‑1 (MQ=255)
                                                                                                   tGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCt                                                   <  1:1027258/150‑1 (MQ=255)
                                                                                                    gCATCTCTTTCAGACGAGACAGTCAAAGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTACATACAGCTGCACTTCTTCACTCACCACTAATTTTACAAGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCtt                                                  >  2:2610122/1‑150 (MQ=255)
                                                                                                     cATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTc                                                 <  2:4131394/150‑1 (MQ=255)
                                                                                                      aTCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCg                                                <  1:2764246/150‑1 (MQ=255)
                                                                                                       tctcTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGc                                               <  2:4076808/150‑1 (MQ=255)
                                                                                                          ctTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGctct                                            <  1:745257/150‑1 (MQ=255)
                                                                                                           ttGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCtctc                                           <  2:1735094/150‑1 (MQ=255)
                                                                                                               aGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATc                                       >  2:700591/1‑150 (MQ=255)
                                                                                                                gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCa                                      <  1:813838/150‑1 (MQ=255)
                                                                                                                gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCa                                      >  2:1543448/1‑150 (MQ=255)
                                                                                                                        aGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTc                              >  1:3964401/1‑150 (MQ=255)
                                                                                                                         ggCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCa                             <  2:1151045/150‑1 (MQ=255)
                                                                                                                         ggCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCa                             <  2:2650569/150‑1 (MQ=255)
                                                                                                                            aaCGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATaa                          >  1:22266/1‑150 (MQ=255)
                                                                                                                            aaCGCTGGAACTCAAACTTCAGCCGATAGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATaa                          <  2:2884237/150‑1 (MQ=255)
                                                                                                                               gCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAAATg                       <  1:2459315/150‑1 (MQ=255)
                                                                                                                               gCTGGAACTCAAACTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAAATg                       <  1:1388852/150‑1 (MQ=255)
                                                                                                                                cTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTgg                      >  1:4115961/1‑150 (MQ=255)
                                                                                                                                cTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTgg                      >  2:2389341/1‑150 (MQ=255)
                                                                                                                                   gAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTAc                   >  2:225064/1‑150 (MQ=255)
                                                                                                                                       tCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCa               <  2:555259/150‑1 (MQ=255)
                                                                                                                                        cAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTGGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCaa              <  1:399995/150‑1 (MQ=255)
                                                                                                                                         aaaCTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCaaa             >  1:4411438/1‑150 (MQ=255)
                                                                                                                                          aaCTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaac            >  1:2158932/1‑150 (MQ=255)
                                                                                                                                           aCTTCAGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaaca           >  2:3871940/1‑150 (MQ=255)
                                                                                                                                           aCTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaaca           >  1:3700600/1‑150 (MQ=255)
                                                                                                                                            cTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAaacaa          <  1:2993586/150‑1 (MQ=255)
                                                                                                                                                aGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATg      <  1:3291129/150‑1 (MQ=255)
                                                                                                                                                aGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATg      >  2:1342003/1‑150 (MQ=255)
                                                                                                                                                aGCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATg      >  1:3485300/1‑150 (MQ=255)
                                                                                                                                                 gCCTATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGa     >  2:274379/1‑150 (MQ=255)
                                                                                                                                                 gCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGa     <  2:3337214/150‑1 (MQ=255)
                                                                                                                                                  ccGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGAc    <  1:974636/150‑1 (MQ=255)
                                                                                                                                                  ccGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGAc    <  1:4611646/150‑1 (MQ=255)
                                                                                                                                                   cGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGAcc   <  1:307339/150‑1 (MQ=255)
                                                                                                                                                    gATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCg  >  2:4571385/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAAACTCATCCACCAGCGCAATAAAGCGCCGCGCTTCGCTCTCCATCAACCGCGTCATAACTGGTACATCAAACAACATGACCG  >  CP009273/3372170‑3372467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A