Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,395,059 C→A 14.3% V545F (GTC→TTC)  csrD ← targeting factor for csrBC sRNA degradation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,395,0590CA14.3% 933.7 / 84.8 287V545F (GTC→TTC) csrDtargeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand):  ref base C (95/150);  new base A (9/33);  total (104/183)
Fisher's exact test for biased strand distribution p-value = 3.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGCCAACGCT  >  CP009273/3394910‑3395208
                                                                                                                                                     |                                                                                                                                                     
ggAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAc                                                                                                                                                       >  1:3161608/1‑150 (MQ=255)
   gCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGc                                                                                                                                                    <  2:50717/150‑1 (MQ=255)
    cAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCt                                                                                                                                                   <  2:4214547/150‑1 (MQ=255)
    cAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCt                                                                                                                                                   <  1:3034382/150‑1 (MQ=255)
    cAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCt                                                                                                                                                   <  1:1982535/150‑1 (MQ=255)
    cAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCt                                                                                                                                                   <  2:1826472/150‑1 (MQ=255)
     aGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTa                                                                                                                                                  >  1:3728585/1‑150 (MQ=255)
        cTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTAccc                                                                                                                                               <  1:2256522/150‑1 (MQ=255)
         ttCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCg                                                                                                                                              <  1:3391173/150‑1 (MQ=255)
            caccaGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTAc                                                                                                                                           >  2:2812780/1‑150 (MQ=255)
               caGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTAcccc                                                                                                                                        >  2:2941366/1‑150 (MQ=255)
                 ggCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTa                                                                                                                                      >  1:1714769/1‑150 (MQ=255)
                   cTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTaaa                                                                                                                                    <  2:3576908/150‑1 (MQ=255)
                    tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAg                                                                                                                                   >  2:65601/1‑150 (MQ=255)
                     ttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGc                                                                                                                                  <  2:4022419/150‑1 (MQ=255)
                        aaCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAtt                                                                                                                               <  1:1015186/150‑1 (MQ=255)
                        aaCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAtt                                                                                                                               <  2:338282/150‑1 (MQ=255)
                         aCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTc                                                                                                                              >  1:831590/1‑150 (MQ=255)
                         aCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTc                                                                                                                              <  2:2491920/150‑1 (MQ=255)
                            agcagcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACt                                                                                                                           >  2:1716022/1‑150 (MQ=255)
                              cagcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTaa                                                                                                                         <  1:1767903/150‑1 (MQ=255)
                               agcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTaaa                                                                                                                        >  1:2505198/1‑150 (MQ=255)
                                gcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAAc                                                                                                                       <  1:1779686/150‑1 (MQ=255)
                                gcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAAc                                                                                                                       <  1:4110122/150‑1 (MQ=255)
                                  tGGTTCTCCTTTCGCTTCTCACTGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGa                                                                                                                     <  2:1780814/150‑1 (MQ=255)
                                  tGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGa                                                                                                                     >  2:309813/1‑150 (MQ=255)
                                  tGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGa                                                                                                                     <  2:3797828/150‑1 (MQ=255)
                                  tGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGa                                                                                                                     <  2:1980672/150‑1 (MQ=255)
                                    gTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAAt                                                                                                                   <  1:1168126/150‑1 (MQ=255)
                                         ccGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAg                                                                                                              >  1:4167617/1‑150 (MQ=255)
                                           gTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGt                                                                                                            >  2:1984235/1‑150 (MQ=255)
                                            ttCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGtt                                                                                                           <  1:4507531/150‑1 (MQ=255)
                                            ttCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGtt                                                                                                           >  1:973966/1‑150 (MQ=255)
                                             tCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTg                                                                                                          <  1:1327827/150‑1 (MQ=255)
                                              cGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGt                                                                                                         <  1:1500909/150‑1 (MQ=255)
                                               gCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTa                                                                                                        <  1:26685/150‑1 (MQ=255)
                                                cTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaa                                                                                                       <  2:2286143/150‑1 (MQ=255)
                                                cTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaa                                                                                                       <  2:758713/150‑1 (MQ=255)
                                                cTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaa                                                                                                       <  2:1018330/150‑1 (MQ=255)
                                                 ttCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaaa                                                                                                      <  1:3922154/150‑1 (MQ=255)
                                                 ttCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaaa                                                                                                      <  1:4569997/150‑1 (MQ=255)
                                                      aaTGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACt                                                                                                 <  2:1605906/150‑1 (MQ=255)
                                                        tGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGa                                                                                               <  2:369625/150‑1 (MQ=255)
                                                        tGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGa                                                                                               <  2:1113854/150‑1 (MQ=255)
                                                          tttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGata                                                                                             >  1:750740/1‑150 (MQ=255)
                                                           ttCTGACCAGCCCGGTATGGAGCTTGATTAACTCAACATTATGTTATTTGATCCAACTGGTACTTACCAGCGACAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGatat                                                                                            <  1:3215551/150‑1 (MQ=255)
                                                           ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACCGGTTGTAAACGACTGatat                                                                                            <  2:263521/150‑1 (MQ=255)
                                                           ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTTTAAACGACTGatat                                                                                            <  1:2787559/150‑1 (MQ=255)
                                                           ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGatat                                                                                            >  2:1713076/1‑150 (MQ=255)
                                                                   aGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCt                                                                                    >  2:3970154/1‑150 (MQ=255)
                                                                    gCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTa                                                                                   <  2:4341537/150‑1 (MQ=255)
                                                                     ccccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTAc                                                                                  <  2:888449/150‑1 (MQ=255)
                                                                     ccccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTAc                                                                                  <  1:4641304/150‑1 (MQ=255)
                                                                      cccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACa                                                                                 <  2:4259692/150‑1 (MQ=255)
                                                                       ccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACAt                                                                                <  2:1440452/150‑1 (MQ=255)
                                                                        cGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATc                                                                               <  2:744089/150‑1 (MQ=255)
                                                                        cGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATc                                                                               <  1:3304381/150‑1 (MQ=255)
                                                                          gATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCgg                                                                             >  2:4584070/1‑150 (MQ=255)
                                                                             ggAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCct                                                                          >  1:4387007/1‑150 (MQ=255)
                                                                               aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCctct                                                                        <  2:1682262/150‑1 (MQ=255)
                                                                                gCTTGAGTAACTAAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCCGCCTGGTTGAAGGCTACCCGAACCCCTAAAGCATTCACTAAACGAATAACCGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTg                                                                       <  1:4464600/150‑1 (MQ=255)
                                                                                 cTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGc                                                                      >  2:2041881/1‑150 (MQ=255)
                                                                                  ttGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCa                                                                     >  1:3784967/1‑150 (MQ=255)
                                                                                      gTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGtt                                                                 <  2:2444789/150‑1 (MQ=255)
                                                                                      gTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGtt                                                                 >  2:4442108/1‑150 (MQ=255)
                                                                                       tAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTc                                                                <  2:3898885/150‑1 (MQ=255)
                                                                                       tAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTc                                                                <  2:2505198/150‑1 (MQ=255)
                                                                                        aaCTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATACCAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCa                                                               <  1:2183580/150‑1 (MQ=255)
                                                                                         aCTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCaa                                                              <  1:657052/150‑1 (MQ=255)
                                                                                           tCAACATTAAGTTATTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCaata                                                            <  1:2069416/150‑1 (MQ=255)
                                                                                               cATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTa                                                        <  2:4088890/150‑1 (MQ=255)
                                                                                                aTTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTAt                                                       <  2:3347338/150‑1 (MQ=255)
                                                                                                     gTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGtt                                                  >  2:3446597/1‑150 (MQ=255)
                                                                                                     gTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGtt                                                  <  1:2119996/150‑1 (MQ=255)
                                                                                                      ttCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGttt                                                 <  1:816545/150‑1 (MQ=255)
                                                                                                       tCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGtttt                                                <  2:1402919/150‑1 (MQ=255)
                                                                                                           tGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGtt                                            <  1:1033964/150‑1 (MQ=255)
                                                                                                            gATCCAACTGGTACTTACCAGCGTCAAACCCGCCTTGTTGACGGCTACCCGTACACCTAAAGCTTTCACTAAACTAATAACAGGTTGTAAAAGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTtg                                           <  2:1171512/150‑1 (MQ=255)
                                                                                                              tCCAACTGGTACTTACCAGCGTCAAACCAGNCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTtgtg                                         >  1:4286328/1‑150 (MQ=255)
                                                                                                                cAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAt                                       <  2:2378352/150‑1 (MQ=255)
                                                                                                                cAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAt                                       <  2:286875/150‑1 (MQ=255)
                                                                                                                cAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAt                                       <  2:454047/150‑1 (MQ=255)
                                                                                                                 aaCTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAtt                                      <  2:4473806/150‑1 (MQ=255)
                                                                                                                   cTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAtttt                                    >  1:2491898/1‑150 (MQ=255)
                                                                                                                      gTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTca                                 >  2:3786138/1‑150 (MQ=255)
                                                                                                                         cTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCACGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAt                              >  2:13186/1‑150 (MQ=255)
                                                                                                                         cTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAt                              <  2:2239055/150‑1 (MQ=255)
                                                                                                                         cTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCCAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAt                              <  2:2818701/150‑1 (MQ=255)
                                                                                                                          ttACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAtt                             >  1:3582928/1‑150 (MQ=255)
                                                                                                                             ccAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCa                          >  1:2204559/1‑150 (MQ=255)
                                                                                                                             ccAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCa                          <  2:1065576/150‑1 (MQ=255)
                                                                                                                              cAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTGGTGATTTTTCACATTGCAt                         >  1:1923543/1‑150 (MQ=255)
                                                                                                                               aGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACTCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCAtt                        <  2:3728585/150‑1 (MQ=255)
                                                                                                                                gCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTACAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTa                       <  1:494622/150‑1 (MQ=255)
                                                                                                                                  gTCAAACCAGCCTGGTTGAAGGCTACCCGTACCCATACAGCATACACTAACCGAATAACCGGTTGTACACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAAc                     <  1:3965423/150‑1 (MQ=255)
                                                                                                                                   tCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCGCATTGCATTAACg                    >  1:919348/1‑150 (MQ=255)
                                                                                                                                      aaCCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTAt                 >  1:3238946/1‑150 (MQ=255)
                                                                                                                                              tGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGc         >  2:2231310/1‑150 (MQ=255)
                                                                                                                                                  tGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGCCAAc     >  2:3751394/1‑150 (MQ=255)
                                                                                                                                                     cGGCTACCCGTACCCCTAACGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACATATCGCGCAGCCAACGCt  <  1:3997524/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGCCAACGCT  >  CP009273/3394910‑3395208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A