Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,832 T→C 100% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,8320TC98.1% 1014.2 / 3.1 258N99D (AAC→GAC) rplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (141/112);  total (144/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440685‑3440978
                                                                                                                                                   |                                                                                                                                                  
aTGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAg                                                                                                                                                  >  2:440551/1‑150 (MQ=255)
  gTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGAc                                                                                                                                                <  1:545115/150‑1 (MQ=255)
       gggTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaa                                                                                                                                           <  1:4313342/150‑1 (MQ=255)
       gggTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaa                                                                                                                                           >  2:2304191/1‑150 (MQ=255)
           gATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGaaa                                                                                                                                       >  1:2730695/1‑150 (MQ=255)
           gATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGaaa                                                                                                                                       >  2:4352510/1‑150 (MQ=255)
           gATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGaaa                                                                                                                                       >  2:1776442/1‑150 (MQ=255)
           gATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGaaa                                                                                                                                       >  2:4136248/1‑150 (MQ=255)
             tcttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACg                                                                                                                                     >  1:2258643/1‑150 (MQ=255)
              cttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACgg                                                                                                                                    <  1:1874743/150‑1 (MQ=255)
               ttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAAATACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAATAAACGGa                                                                                                                                   >  1:1441660/1‑150 (MQ=255)
                tctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAc                                                                                                                                  >  1:3740285/1‑150 (MQ=255)
                tctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAc                                                                                                                                  <  1:3292955/150‑1 (MQ=255)
                 ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTATGTAGTAATCATGAAGTTTCGCCATCGTACTACACCAAATTAATTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACt                                                                                                                                 >  1:290937/1‑150 (MQ=255)
                  tcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtt                                                                                                                                >  2:653307/1‑150 (MQ=255)
                   cGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACttt                                                                                                                               <  2:256135/150‑1 (MQ=255)
                      cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                            <  2:1399780/150‑1 (MQ=255)
                            ggACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTc                                                                                                                      <  1:2180542/150‑1 (MQ=255)
                               cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                                                                                   <  2:2773295/150‑1 (MQ=255)
                                   cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                                                                               >  2:2816701/1‑150 (MQ=255)
                                    aTGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATc                                                                                                              <  2:2052985/150‑1 (MQ=255)
                                      gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTa                                                                                                            <  2:3321999/150‑1 (MQ=255)
                                       aCAGAAGTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaa                                                                                                           <  2:2147019/150‑1 (MQ=255)
                                         aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         >  2:3522839/1‑150 (MQ=255)
                                          gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACATTTTTACCGTCTTCGAATCTAAAGc                                                                                                        >  1:4208/1‑150 (MQ=255)
                                             ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTa                                                                                                     >  1:662003/1‑150 (MQ=255)
                                              tgttgtTAATCTCAGTCTTGAGTTTTTTAACTACTTCGTATTTGTAGTAATCATGCAGTTTCGCCATCGTACTAATCCAAATTACTTGTTTGTTTCGCTGTCAGACTTGAAGAAACGGTCTTTTTTTCCGTCTTCGAATCTTTAGCCTac                                                                                                    <  1:1114297/150‑1 (MQ=255)
                                              tGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTac                                                                                                    >  1:820657/1‑150 (MQ=255)
                                                 aGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACg                                                                                                 >  1:554751/1‑150 (MQ=255)
                                                 aGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACg                                                                                                 <  1:3618860/150‑1 (MQ=255)
                                                  gTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACgg                                                                                                <  1:398835/150‑1 (MQ=255)
                                                     aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             >  1:251568/1‑150 (MQ=255)
                                                     aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             <  1:2401596/150‑1 (MQ=255)
                                                      aaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                                                                                            >  2:3519175/1‑150 (MQ=255)
                                                      aaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                                                                                            >  2:148475/1‑150 (MQ=255)
                                                        cTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGcc                                                                                          <  2:751903/150‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        <  1:3092519/150‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        >  2:2804197/1‑150 (MQ=255)
                                                           aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                       >  2:4501250/1‑150 (MQ=255)
                                                            gTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                                                                                      >  1:3402736/1‑150 (MQ=255)
                                                            gTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                                                                                      <  1:3067170/150‑1 (MQ=255)
                                                             tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGcc                                                                                     <  1:3222481/150‑1 (MQ=255)
                                                               aTGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCgg                                                                                   >  1:1006704/1‑150 (MQ=255)
                                                                tGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGt                                                                                  <  2:4391950/150‑1 (MQ=255)
                                                                 gAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGtt                                                                                 <  1:148024/150‑1 (MQ=255)
                                                                 gAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGtt                                                                                 <  2:871352/150‑1 (MQ=255)
                                                                  aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                                >  1:2453195/1‑150 (MQ=255)
                                                                     tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             >  2:125539/1‑150 (MQ=255)
                                                                     tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             <  2:1855143/150‑1 (MQ=255)
                                                                          aaCTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTg                                                                        >  2:4232543/1‑150 (MQ=255)
                                                                          aaCTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTg                                                                        <  2:2040328/150‑1 (MQ=255)
                                                                            ctactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa                                                                      <  1:2583462/150‑1 (MQ=255)
                                                                            ctactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTGTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCAGCATTGaa                                                                      >  2:1648534/1‑150 (MQ=255)
                                                                             tactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg                                                                     >  2:3157789/1‑150 (MQ=255)
                                                                             tactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg                                                                     <  1:4372872/150‑1 (MQ=255)
                                                                               ctTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAt                                                                   >  2:3757094/1‑150 (MQ=255)
                                                                               ctTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAt                                                                   >  1:154196/1‑150 (MQ=255)
                                                                                ttCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt                                                                  >  2:3928843/1‑150 (MQ=255)
                                                                                ttCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt                                                                  <  2:3950491/150‑1 (MQ=255)
                                                                                  cGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc                                                                >  2:2944670/1‑150 (MQ=255)
                                                                                    tCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa                                                              >  1:3240723/1‑150 (MQ=255)
                                                                                        tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACTGTCAGCCTTGCCGGTTGCCGCATTGAATATTGCTACGtt                                                          <  2:2759771/150‑1 (MQ=255)
                                                                                           agtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGa                                                       <  1:1759162/150‑1 (MQ=255)
                                                                                                  aTGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGa                                                <  1:2110873/150‑1 (MQ=255)
                                                                                                   tGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGaa                                               >  2:3226082/1‑150 (MQ=255)
                                                                                                      aGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAg                                            >  2:1357016/1‑150 (MQ=255)
                                                                                                      aGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAg                                            <  2:1952783/150‑1 (MQ=255)
                                                                                                       gTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGc                                           >  1:3027765/1‑150 (MQ=255)
                                                                                                        tttCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCg                                          <  1:687101/150‑1 (MQ=255)
                                                                                                          tCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGc                                        >  2:1441442/1‑150 (MQ=255)
                                                                                                           cGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGct                                       <  2:4293385/150‑1 (MQ=255)
                                                                                                            gCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGctt                                      >  2:1555958/1‑150 (MQ=255)
                                                                                                                 cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                 >  1:1603579/1‑150 (MQ=255)
                                                                                                                    actactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCa                              >  1:4242650/1‑150 (MQ=255)
                                                                                                                     ctactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCaa                             >  2:3879017/1‑150 (MQ=255)
                                                                                                                      tactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAAc                            <  2:1343491/150‑1 (MQ=255)
                                                                                                                      tactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAAc                            <  2:1343321/150‑1 (MQ=255)
                                                                                                                      tactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCAAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTAGGAAACCTGAATAGCGGCTTCTTTTTCAAc                            >  2:581346/1‑150 (MQ=255)
                                                                                                                         tCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGAt                         <  2:942666/150‑1 (MQ=255)
                                                                                                                         tCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGAt                         >  2:2920236/1‑150 (MQ=255)
                                                                                                                          ccAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATg                        <  2:3673604/150‑1 (MQ=255)
                                                                                                                          ccAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATg                        <  1:1229853/150‑1 (MQ=255)
                                                                                                                          ccAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATg                        <  2:1691706/150‑1 (MQ=255)
                                                                                                                            aaaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                      >  1:1044512/1‑150 (MQ=255)
                                                                                                                            aaaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                      >  2:2437018/1‑150 (MQ=255)
                                                                                                                             aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     <  2:3127714/150‑1 (MQ=255)
                                                                                                                             aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     >  2:2037370/1‑150 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGGTGCCAcc                   <  2:3395882/150‑1 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAcc                   <  2:3215139/150‑1 (MQ=255)
                                                                                                                               ttACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAcc                   <  2:1929882/150‑1 (MQ=255)
                                                                                                                                tACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAccc                  >  1:912536/1‑150 (MQ=255)
                                                                                                                                 aCTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCg                 >  1:3680840/1‑150 (MQ=255)
                                                                                                                                  cTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCgg                >  2:1869402/1‑150 (MQ=255)
                                                                                                                                     gATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg             <  1:376342/150‑1 (MQ=255)
                                                                                                                                     gATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg             <  1:2940261/150‑1 (MQ=255)
                                                                                                                                     gATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg             >  2:3152325/1‑150 (MQ=255)
                                                                                                                                         gTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTc         <  2:466784/150‑1 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  1:4041936/1‑150 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  2:2407332/1‑150 (MQ=255)
                                                                                                                                             cGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAggg     <  1:3136037/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440685‑3440978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGG  >  CP009273/3440684‑3440979
                                                                                                                                                    |                                                                                                                                                   
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCA                                                                                                                                                    <  SRR19973072.4195652/150‑1 (MQ=60)
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCA                                                                                                                                                    <  SRR19973072.1557344/150‑1 (MQ=60)
   GTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGAC                                                                                                                                                 <  SRR19973072.546402/150‑1 (MQ=60)
   GTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGAC                                                                                                                                                 <  SRR19973072.546411/150‑1 (MQ=60)
        GGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAA                                                                                                                                            <  SRR19973072.4322359/150‑1 (MQ=60)
           TGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAA                                                                                                                                         >  SRR19973072.3072440/1‑150 (MQ=60)
            GATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAA                                                                                                                                        >  SRR19973072.2736521/1‑150 (MQ=60)
            GATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAA                                                                                                                                        >  SRR19973072.1543130/1‑150 (MQ=60)
              TCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACG                                                                                                                                      >  SRR19973072.2263505/1‑150 (MQ=60)
               CTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGG                                                                                                                                     <  SRR19973072.1878771/150‑1 (MQ=60)
                TTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGA                                                                                                                                    >  SRR19973072.1444820/1‑150 (MQ=60)
                TTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAAATACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAATAAACGGA                                                                                                                                    >  SRR19973072.1444768/1‑150 (MQ=60)
                 TCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAC                                                                                                                                   >  SRR19973072.2849821/1‑150 (MQ=60)
                 TCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAC                                                                                                                                   >  SRR19973072.3748152/1‑150 (MQ=60)
                 TCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAC                                                                                                                                   >  SRR19973072.3747708/1‑150 (MQ=60)
                 TCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAC                                                                                                                                   <  SRR19973072.3299942/150‑1 (MQ=60)
                 TCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAC                                                                                                                                   <  SRR19973072.4220049/150‑1 (MQ=60)
                  CTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTATGTAGTAATCATGAAGTTTCGCCATCGTACTACACCAAATTAATTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACT                                                                                                                                  >  SRR19973072.291620/1‑150 (MQ=60)
                   TCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTT                                                                                                                                 >  SRR19973072.2424222/1‑150 (MQ=60)
                   TCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTT                                                                                                                                 >  SRR19973072.1594649/1‑150 (MQ=60)
                   TCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTT                                                                                                                                 >  SRR19973072.1594684/1‑150 (MQ=60)
                    CGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTT                                                                                                                                >  SRR19973072.3909677/1‑150 (MQ=60)
                      ACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTT                                                                                                                              <  SRR19973072.3480110/150‑1 (MQ=60)
                             GGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTC                                                                                                                       <  SRR19973072.2185427/150‑1 (MQ=60)
                             GGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTC                                                                                                                       <  SRR19973072.2185290/150‑1 (MQ=60)
                              GACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCT                                                                                                                      >  SRR19973072.4298949/1‑150 (MQ=60)
                              GACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATAGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAACGTCT                                                                                                                      >  SRR19973072.957987/1‑150 (MQ=60)
                                 TTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCG                                                                                                                   >  SRR19973072.2560490/1‑150 (MQ=60)
                                   GCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAA                                                                                                                 <  SRR19973072.2996120/150‑1 (MQ=60)
                                    CATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAT                                                                                                                >  SRR19973072.4351965/1‑150 (MQ=60)
                                      TGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCT                                                                                                              <  SRR19973072.2154511/150‑1 (MQ=60)
                                      TGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCT                                                                                                              <  SRR19973072.852800/150‑1 (MQ=60)
                                       GACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTA                                                                                                             >  SRR19973072.3720722/1‑150 (MQ=60)
                                        ACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAA                                                                                                            >  SRR19973072.1770236/1‑150 (MQ=60)
                                        ACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAA                                                                                                            >  SRR19973072.1770141/1‑150 (MQ=60)
                                           GAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACATTTTTACCGTCTTCGAATCTAAAGC                                                                                                         >  SRR19973072.4220/1‑150 (MQ=60)
                                              TTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTA                                                                                                      >  SRR19973072.663491/1‑150 (MQ=60)
                                               TGTTGTTAATCTCAGTCTTGAGTTTTTTAACTACTTCGTATTTGTAGTAATCATGCAGTTTCGCCATCGTACTAATCCAAATTACTTGTTTGTTTCGCTGTCAGACTTGAAGAAACGGTCTTTTTTTCCGTCTTCGAATCTTTAGCCTAC                                                                                                     <  SRR19973072.1116649/150‑1 (MQ=60)
                                               TGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTAC                                                                                                     >  SRR19973072.822421/1‑150 (MQ=60)
                                               TGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTAC                                                                                                     <  SRR19973072.1116600/150‑1 (MQ=60)
                                                GTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACA                                                                                                    <  SRR19973072.2005008/150‑1 (MQ=60)
                                                GTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACA                                                                                                    <  SRR19973072.4339483/150‑1 (MQ=60)
                                                  AGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACG                                                                                                  >  SRR19973072.556053/1‑150 (MQ=60)
                                                  AGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACG                                                                                                  >  SRR19973072.556059/1‑150 (MQ=60)
                                                  AGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACG                                                                                                  <  SRR19973072.3626482/150‑1 (MQ=60)
                                                  AGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACG                                                                                                  <  SRR19973072.3297842/150‑1 (MQ=60)
                                                   GTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGG                                                                                                 <  SRR19973072.399668/150‑1 (MQ=60)
                                                   GTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGG                                                                                                 <  SRR19973072.399716/150‑1 (MQ=60)
                                                   GTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGG                                                                                                 <  SRR19973072.399730/150‑1 (MQ=60)
                                                   GTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGG                                                                                                 <  SRR19973072.399779/150‑1 (MQ=60)
                                                      AAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCA                                                                                              <  SRR19973072.2406780/150‑1 (MQ=60)
                                                      AAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCA                                                                                              >  SRR19973072.252174/1‑150 (MQ=60)
                                                      AAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCA                                                                                              <  SRR19973072.2406790/150‑1 (MQ=60)
                                                          TCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCT                                                                                          <  SRR19973072.4150865/150‑1 (MQ=60)
                                                           CAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTT                                                                                         <  SRR19973072.3099040/150‑1 (MQ=60)
                                                             GTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGC                                                                                       >  SRR19973072.3409924/1‑150 (MQ=60)
                                                             GTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGC                                                                                       <  SRR19973072.3073674/150‑1 (MQ=60)
                                                             GTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGC                                                                                       <  SRR19973072.3073648/150‑1 (MQ=60)
                                                              TCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCC                                                                                      <  SRR19973072.3229303/150‑1 (MQ=60)
                                                                ATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGG                                                                                    >  SRR19973072.1008898/1‑150 (MQ=60)
                                                                ATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGG                                                                                    >  SRR19973072.1008986/1‑150 (MQ=60)
                                                                ATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGG                                                                                    >  SRR19973072.4593010/1‑150 (MQ=60)
                                                                  GAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTT                                                                                  <  SRR19973072.148398/150‑1 (MQ=60)
                                                                   AGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTG                                                                                 >  SRR19973072.2458526/1‑150 (MQ=60)
                                                                   AGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTG                                                                                 >  SRR19973072.3244698/1‑150 (MQ=60)
                                                                      TTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCG                                                                              >  SRR19973072.574571/1‑150 (MQ=60)
                                                                           AACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTG                                                                         <  SRR19973072.424004/150‑1 (MQ=60)
                                                                            ACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGA                                                                        >  SRR19973072.1339535/1‑150 (MQ=60)
                                                                             CTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAA                                                                       <  SRR19973072.2589041/150‑1 (MQ=60)
                                                                              TACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAG                                                                      <  SRR19973072.4382060/150‑1 (MQ=60)
                                                                              TACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAG                                                                      <  SRR19973072.352961/150‑1 (MQ=60)
                                                                                CTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAT                                                                    >  SRR19973072.671945/1‑150 (MQ=60)
                                                                                CTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAT                                                                    >  SRR19973072.154592/1‑150 (MQ=60)
                                                                                  TCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTG                                                                  <  SRR19973072.2451542/150‑1 (MQ=60)
                                                                                   CGTATTTGTAGTACTCATGCAGTTTCGCATTCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGATTCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGC                                                                 <  SRR19973072.305729/150‑1 (MQ=60)
                                                                                     TCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTA                                                               >  SRR19973072.3247599/1‑150 (MQ=60)
                                                                                     TCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTA                                                               >  SRR19973072.3247597/1‑150 (MQ=60)
                                                                                           TAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGG                                                         <  SRR19973072.818469/150‑1 (MQ=60)
                                                                                            AGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGA                                                        <  SRR19973072.1762997/150‑1 (MQ=60)
                                                                                            AGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGA                                                        <  SRR19973072.1763007/150‑1 (MQ=60)
                                                                                              TAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAA                                                      >  SRR19973072.229589/1‑150 (MQ=60)
                                                                                                 TCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCT                                                   >  SRR19973072.3316694/1‑150 (MQ=60)
                                                                                                   ATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGA                                                 <  SRR19973072.2115474/150‑1 (MQ=60)
                                                                                                   ATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGA                                                 <  SRR19973072.2115521/150‑1 (MQ=60)
                                                                                                    TGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAA                                                >  SRR19973072.315423/1‑150 (MQ=60)
                                                                                                        GTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGC                                            >  SRR19973072.3034181/1‑150 (MQ=60)
                                                                                                        GTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGC                                            <  SRR19973072.3758367/150‑1 (MQ=60)
                                                                                                         TTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCG                                           <  SRR19973072.2688355/150‑1 (MQ=60)
                                                                                                         TTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCG                                           <  SRR19973072.688622/150‑1 (MQ=60)
                                                                                                              CCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTC                                      >  SRR19973072.781299/1‑150 (MQ=60)
                                                                                                                  CGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTT                                  >  SRR19973072.2867563/1‑150 (MQ=60)
                                                                                                                  CGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTT                                  >  SRR19973072.1607034/1‑150 (MQ=60)
                                                                                                                   GTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTT                                 >  SRR19973072.3115987/1‑150 (MQ=60)
                                                                                                                     ACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCA                               >  SRR19973072.1278669/1‑150 (MQ=60)
                                                                                                                     ACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCA                               >  SRR19973072.2524509/1‑150 (MQ=60)
                                                                                                                     ACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCA                               >  SRR19973072.4251480/1‑150 (MQ=60)
                                                                                                                         CTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGA                           >  SRR19973072.2505358/1‑150 (MQ=60)
                                                                                                                         CTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGA                           >  SRR19973072.2917217/1‑150 (MQ=60)
                                                                                                                           CCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATG                         <  SRR19973072.1232481/150‑1 (MQ=60)
                                                                                                                           CCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATG                         <  SRR19973072.522355/150‑1 (MQ=60)
                                                                                                                             AAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCC                       >  SRR19973072.1046795/1‑150 (MQ=60)
                                                                                                                             AAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCC                       >  SRR19973072.1046763/1‑150 (MQ=60)
                                                                                                                                 TACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCC                   >  SRR19973072.914510/1‑150 (MQ=60)
                                                                                                                                  ACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCG                  >  SRR19973072.3688244/1‑150 (MQ=60)
                                                                                                                                  ACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCG                  >  SRR19973072.3688598/1‑150 (MQ=60)
                                                                                                                                   CTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGG                 <  SRR19973072.1495128/150‑1 (MQ=60)
                                                                                                                                     TGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTT               <  SRR19973072.1145347/150‑1 (MQ=60)
                                                                                                                                     TGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTT               <  SRR19973072.3141139/150‑1 (MQ=60)
                                                                                                                                      GATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTG              <  SRR19973072.2946550/150‑1 (MQ=60)
                                                                                                                                      GATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTG              <  SRR19973072.2946549/150‑1 (MQ=60)
                                                                                                                                      GATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTG              <  SRR19973072.377155/150‑1 (MQ=60)
                                                                                                                                         AGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTT           >  SRR19973072.3250054/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG       >  SRR19973072.4050416/1‑150 (MQ=60)
                                                                                                                                              CGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGG      <  SRR19973072.3142650/150‑1 (MQ=60)
                                                                                                                                                  GTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGG  <  SRR19973072.2820759/150‑1 (MQ=60)
                                                                                                                                                  GTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCAAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGG  <  SRR19973072.246545/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGG  >  CP009273/3440684‑3440979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑