Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,832 T→C 92.2% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,8320TC92.2% 708.5 / 33.5 205N99D (AAC→GAC) rplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base T (10/6);  new base C (117/72);  total (127/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 205 total aligned reads displayed.

TGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440686‑3440978
                                                                                                                                                  |                                                                                                                                                  
tGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGa                                                                                                                                                 <  2:4773579/150‑1 (MQ=255)
    cAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTg                                                                                                                                             <  1:1682212/150‑1 (MQ=255)
       ggTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTgaag                                                                                                                                          <  2:234275/150‑1 (MQ=255)
             cttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACgg                                                                                                                                    <  2:1133461/150‑1 (MQ=255)
             cttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACgg                                                                                                                                    <  2:2418336/150‑1 (MQ=255)
              ttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGa                                                                                                                                   >  2:162403/1‑150 (MQ=255)
                ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACt                                                                                                                                 <  1:4125800/150‑1 (MQ=255)
                  cGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACttt                                                                                                                               >  2:1453474/1‑150 (MQ=255)
                    aCCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACttttt                                                                                                                             <  1:1915237/150‑1 (MQ=255)
                      ccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTa                                                                                                                           >  2:2336923/1‑150 (MQ=255)
                       cGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCGTTGTAGTACTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAc                                                                                                                          >  1:547610/1‑150 (MQ=255)
                         aGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCg                                                                                                                        >  2:3171562/1‑150 (MQ=255)
                           ggACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTc                                                                                                                      >  1:3718693/1‑150 (MQ=255)
                            gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     <  2:4311772/150‑1 (MQ=255)
                                tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                                                                                 >  1:1625640/1‑150 (MQ=255)
                                  cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCTAAt                                                                                                               >  1:3901216/1‑150 (MQ=255)
                                  cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                                                                               <  1:4008776/150‑1 (MQ=255)
                                     gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTa                                                                                                            >  2:2763120/1‑150 (MQ=255)
                                      aCAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaa                                                                                                           >  2:87236/1‑150 (MQ=255)
                                       cAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                          >  1:3312729/1‑150 (MQ=255)
                                        aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         >  1:3121126/1‑150 (MQ=255)
                                        aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         <  2:608409/150‑1 (MQ=255)
                                        aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         >  2:3115919/1‑150 (MQ=255)
                                         gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGc                                                                                                        <  2:730771/150‑1 (MQ=255)
                                           aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCt                                                                                                      <  1:3504854/150‑1 (MQ=255)
                                           aTTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCt                                                                                                      <  1:1293772/150‑1 (MQ=255)
                                             tGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTac                                                                                                    <  2:4523966/150‑1 (MQ=255)
                                              gTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTaca                                                                                                   <  2:4388137/150‑1 (MQ=255)
                                              gTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTaca                                                                                                   >  2:3060602/1‑150 (MQ=255)
                                              gTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTaca                                                                                                   <  1:3290764/150‑1 (MQ=255)
                                               tAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTacac                                                                                                  <  1:3581930/150‑1 (MQ=255)
                                                  ttAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGt                                                                                               >  2:1437505/1‑150 (MQ=255)
                                                   tAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTc                                                                                              >  1:3028110/1‑150 (MQ=255)
                                                    aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             >  1:842722/1‑150 (MQ=255)
                                                    aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACATGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             >  1:2808722/1‑150 (MQ=255)
                                                        tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                         <  1:3792187/150‑1 (MQ=255)
                                                        tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                         <  2:1315103/150‑1 (MQ=255)
                                                        tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                         >  2:2659815/1‑150 (MQ=255)
                                                        tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                         <  2:779647/150‑1 (MQ=255)
                                                         cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        >  1:4632464/1‑150 (MQ=255)
                                                          aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                       >  1:1248843/1‑150 (MQ=255)
                                                               tGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGt                                                                                  >  1:4622274/1‑150 (MQ=255)
                                                                 aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                                <  2:3331019/150‑1 (MQ=255)
                                                                 aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                                <  2:4079752/150‑1 (MQ=255)
                                                                 aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                                                                                <  2:4024430/150‑1 (MQ=255)
                                                                  gTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGc                                                                               <  2:3090658/150‑1 (MQ=255)
                                                                    tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             >  1:447478/1‑150 (MQ=255)
                                                                    tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             >  2:1312857/1‑150 (MQ=255)
                                                                      tttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCa                                                                           <  1:2651711/150‑1 (MQ=255)
                                                                        tAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAtt                                                                         >  1:1767081/1‑150 (MQ=255)
                                                                                  gTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt                                                               <  1:3756082/150‑1 (MQ=255)
                                                                                   tCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa                                                              <  2:2050091/150‑1 (MQ=255)
                                                                                   tCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTGGCCGGTTGCCGCATTGAAGAGTGCTa                                                              >  2:4371537/1‑150 (MQ=255)
                                                                                    cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAc                                                             <  1:2789207/150‑1 (MQ=255)
                                                                                    cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAc                                                             >  1:1513592/1‑150 (MQ=255)
                                                                                     tttGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg                                                            >  2:1170989/1‑150 (MQ=255)
                                                                                       tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCCTTGAAGATTGCTACGtt                                                          >  1:910323/1‑150 (MQ=255)
                                                                                       tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGtt                                                          <  1:4720142/150‑1 (MQ=255)
                                                                                         tagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTgg                                                        >  1:4234102/1‑150 (MQ=255)
                                                                                          agtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGa                                                       >  1:977643/1‑150 (MQ=255)
                                                                                           gtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaa                                                      >  1:1016611/1‑150 (MQ=255)
                                                                                           gtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaa                                                      >  2:4315828/1‑150 (MQ=255)
                                                                                              aTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAcc                                                   >  2:2922530/1‑150 (MQ=255)
                                                                                               tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCt                                                  >  1:1849417/1‑150 (MQ=255)
                                                                                               tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCt                                                  <  1:310914/150‑1 (MQ=255)
                                                                                                cATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTg                                                 <  2:4306918/150‑1 (MQ=255)
                                                                                                    cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAGTGCTACGTTGGAAACCTGAATa                                             >  2:667548/1‑150 (MQ=255)
                                                                                                      gTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGc                                           <  2:244027/150‑1 (MQ=255)
                                                                                                      gTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGc                                           >  2:2848737/1‑150 (MQ=255)
                                                                                                       tttCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCg                                          <  1:2907781/150‑1 (MQ=255)
                                                                                                            ccATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttc                                     <  1:1529421/150‑1 (MQ=255)
                                                                                                                cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAACCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                 <  2:3361121/150‑1 (MQ=255)
                                                                                                                 gTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttttt                                >  1:120387/1‑150 (MQ=255)
                                                                                                                 gTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttttt                                >  1:2638953/1‑150 (MQ=255)
                                                                                                                  tactacTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                               >  2:156252/1‑150 (MQ=255)
                                                                                                                  tactacTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                               >  2:4256018/1‑150 (MQ=255)
                                                                                                                   actactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCa                              >  2:793486/1‑150 (MQ=255)
                                                                                                                      actCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACg                           >  2:635082/1‑150 (MQ=255)
                                                                                                                          cAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGc                       >  1:3838050/1‑150 (MQ=255)
                                                                                                                          cAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGc                       >  1:2845941/1‑150 (MQ=255)
                                                                                                                           aaaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTTCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTAAACGATGcc                      >  1:418911/1‑150 (MQ=255)
                                                                                                                            aaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     >  2:4052552/1‑150 (MQ=255)
                                                                                                                            aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     >  2:3011628/1‑150 (MQ=255)
                                                                                                                            aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     >  2:2576215/1‑150 (MQ=255)
                                                                                                                            aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     <  1:4412057/150‑1 (MQ=255)
                                                                                                                              ttACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAcc                   >  2:3491781/1‑150 (MQ=255)
                                                                                                                                aCTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCg                 >  1:2569662/1‑150 (MQ=255)
                                                                                                                                aCTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCg                 <  1:2291964/150‑1 (MQ=255)
                                                                                                                                 cTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCgg                >  2:2006814/1‑150 (MQ=255)
                                                                                                                                  ttGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggt               >  1:742648/1‑150 (MQ=255)
                                                                                                                                         tttCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCa        >  2:3784376/1‑150 (MQ=255)
                                                                                                                                          ttCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAg       >  2:2970412/1‑150 (MQ=255)
                                                                                                                                          ttCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAg       >  1:2138425/1‑150 (MQ=255)
                                                                                                                                           tCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  1:468590/1‑150 (MQ=255)
                                                                                                                                           tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  2:3588770/1‑150 (MQ=255)
                                                                                                                                           tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  1:800127/1‑150 (MQ=255)
                                                                                                                                           tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  2:1251635/1‑150 (MQ=255)
                                                                                                                                           tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      >  1:3186717/1‑150 (MQ=255)
                                                                                                                                              cTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGcc   >  2:2220016/1‑150 (MQ=255)
                                                                                                                                               tGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCg  <  1:4551677/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440686‑3440978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440684‑3440978
                                                                                                                                                    |                                                                                                                                                  
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCA                                                                                                                                                   <  SRR19973078.3593278/150‑1 (MQ=60)
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCA                                                                                                                                                   >  SRR19973078.269746/1‑150 (MQ=60)
   GTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGAC                                                                                                                                                >  SRR19973078.3845964/1‑150 (MQ=60)
    TTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACT                                                                                                                                               >  SRR19973078.227058/1‑150 (MQ=60)
      CAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTG                                                                                                                                             <  SRR19973078.1692461/150‑1 (MQ=60)
      CAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTG                                                                                                                                             <  SRR19973078.1692432/150‑1 (MQ=60)
                  CTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACT                                                                                                                                 <  SRR19973078.4149376/150‑1 (MQ=60)
                   TCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTT                                                                                                                                >  SRR19973078.3129777/1‑150 (MQ=60)
                      ACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTT                                                                                                                             <  SRR19973078.1926540/150‑1 (MQ=60)
                      ACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTT                                                                                                                             >  SRR19973078.2652158/1‑150 (MQ=60)
                      ACCAGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTAATGAGTTTTTTAACTACTTCGTATTTGTAGTAATCATGAAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTTGACTTGAAGAAACGGACTTTTT                                                                                                                             <  SRR19973078.4322174/150‑1 (MQ=60)
                         CGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCGTTGTAGTACTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAC                                                                                                                          >  SRR19973078.551230/1‑150 (MQ=60)
                             GGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTC                                                                                                                      <  SRR19973078.469121/150‑1 (MQ=60)
                             GGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTC                                                                                                                      >  SRR19973078.3757932/1‑150 (MQ=60)
                             GGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTC                                                                                                                      >  SRR19973078.3740001/1‑150 (MQ=60)
                              GACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCT                                                                                                                     <  SRR19973078.2851066/150‑1 (MQ=60)
                                 TTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCG                                                                                                                  <  SRR19973078.4517650/150‑1 (MQ=60)
                                 TTGCATGACAGAATTGGAGTTACACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCG                                                                                                                  <  SRR19973078.3628604/150‑1 (MQ=60)
                                  TGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGA                                                                                                                 >  SRR19973078.1635439/1‑150 (MQ=60)
                                    CATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCTAAT                                                                                                               >  SRR19973078.3923436/1‑150 (MQ=60)
                                    CATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAT                                                                                                               <  SRR19973078.4031437/150‑1 (MQ=60)
                                        ACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAA                                                                                                           >  SRR19973078.4615375/1‑150 (MQ=60)
                                         CAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAA                                                                                                          >  SRR19973078.799358/1‑150 (MQ=60)
                                         CAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAA                                                                                                          >  SRR19973078.3331644/1‑150 (MQ=60)
                                          AGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAG                                                                                                         >  SRR19973078.3138652/1‑150 (MQ=60)
                                          AGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAG                                                                                                         >  SRR19973078.3254220/1‑150 (MQ=60)
                                          AGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAG                                                                                                         >  SRR19973078.850989/1‑150 (MQ=60)
                                             ATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCT                                                                                                      <  SRR19973078.3524758/150‑1 (MQ=60)
                                             ATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCT                                                                                                      <  SRR19973078.1301223/150‑1 (MQ=60)
                                             ATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCT                                                                                                      <  SRR19973078.1301855/150‑1 (MQ=60)
                                                GTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACA                                                                                                   <  SRR19973078.3309509/150‑1 (MQ=60)
                                                 TAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACAC                                                                                                  <  SRR19973078.3602058/150‑1 (MQ=60)
                                                     TAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTC                                                                                              >  SRR19973078.3045326/1‑150 (MQ=60)
                                                     TAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTC                                                                                              >  SRR19973078.681073/1‑150 (MQ=60)
                                                      AAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCA                                                                                             >  SRR19973078.847795/1‑150 (MQ=60)
                                                      AAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACATGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAATCCTACACGGTCA                                                                                             >  SRR19973078.2824420/1‑150 (MQ=60)
                                                          TCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCT                                                                                         <  SRR19973078.3813959/150‑1 (MQ=60)
                                                          TCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCT                                                                                         <  SRR19973078.3813875/150‑1 (MQ=60)
                                                           CAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTT                                                                                        <  SRR19973078.4107696/150‑1 (MQ=60)
                                                           CAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTT                                                                                        >  SRR19973078.4658707/1‑150 (MQ=60)
                                                            AGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTG                                                                                       >  SRR19973078.1256563/1‑150 (MQ=60)
                                                              TCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCC                                                                                     >  SRR19973078.1465405/1‑150 (MQ=60)
                                                              TCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCC                                                                                     >  SRR19973078.3026935/1‑150 (MQ=60)
                                                                 TGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGT                                                                                  >  SRR19973078.4641930/1‑150 (MQ=60)
                                                                 TGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGT                                                                                  >  SRR19973078.4648458/1‑150 (MQ=60)
                                                                 TGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGT                                                                                  >  SRR19973078.4648603/1‑150 (MQ=60)
                                                                   AGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTG                                                                                <  SRR19973078.1763403/150‑1 (MQ=60)
                                                                      TTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCG                                                                             >  SRR19973078.450322/1‑150 (MQ=60)
                                                                      TTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCG                                                                             >  SRR19973078.450309/1‑150 (MQ=60)
                                                                        TTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCA                                                                           <  SRR19973078.2667451/150‑1 (MQ=60)
                                                                        TTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCA                                                                           <  SRR19973078.2666828/150‑1 (MQ=60)
                                                                        TTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCA                                                                           <  SRR19973078.2666781/150‑1 (MQ=60)
                                                                          TAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATT                                                                         >  SRR19973078.1777826/1‑150 (MQ=60)
                                                                            ACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGA                                                                       >  SRR19973078.3961066/1‑150 (MQ=60)
                                                                            ACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGA                                                                       >  SRR19973078.1855101/1‑150 (MQ=60)
                                                                             CTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAA                                                                      >  SRR19973078.1625809/1‑150 (MQ=60)
                                                                                    GTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCT                                                               >  SRR19973078.549462/1‑150 (MQ=60)
                                                                                    GTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCT                                                               <  SRR19973078.3777650/150‑1 (MQ=60)
                                                                                     TCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTA                                                              >  SRR19973078.835952/1‑150 (MQ=60)
                                                                                      CTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAC                                                             <  SRR19973078.2804985/150‑1 (MQ=60)
                                                                                      CTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAC                                                             <  SRR19973078.2804727/150‑1 (MQ=60)
                                                                                      CTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAC                                                             >  SRR19973078.2015784/1‑150 (MQ=60)
                                                                                      CTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAC                                                             >  SRR19973078.1522750/1‑150 (MQ=60)
                                                                                      CTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTAC                                                             >  SRR19973078.1522747/1‑150 (MQ=60)
                                                                                         TGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCCTTGAAGATTGCTACGTT                                                          >  SRR19973078.915997/1‑150 (MQ=60)
                                                                                         TGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTT                                                          >  SRR19973078.3319970/1‑150 (MQ=60)
                                                                                         TGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTT                                                          <  SRR19973078.4746723/150‑1 (MQ=60)
                                                                                           TAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGG                                                        >  SRR19973078.4258195/1‑150 (MQ=60)
                                                                                           TAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGG                                                        >  SRR19973078.4258209/1‑150 (MQ=60)
                                                                                            AGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGA                                                       >  SRR19973078.983780/1‑150 (MQ=60)
                                                                                            AGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGA                                                       >  SRR19973078.983753/1‑150 (MQ=60)
                                                                                             GTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAA                                                      >  SRR19973078.1022949/1‑150 (MQ=60)
                                                                                                 TCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCT                                                  >  SRR19973078.1860493/1‑150 (MQ=60)
                                                                                                 TCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCT                                                  <  SRR19973078.313011/150‑1 (MQ=60)
                                                                                                   ATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGA                                                >  SRR19973078.3118861/1‑150 (MQ=60)
                                                                                                         TTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCG                                          <  SRR19973078.2924353/150‑1 (MQ=60)
                                                                                                              CCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTC                                     <  SRR19973078.1538617/150‑1 (MQ=60)
                                                                                                                   GTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGTCGCATTGAAGATTGCTACTTTGGAAACCTGAATAGCGGCTTCTTTTT                                >  SRR19973078.2955560/1‑150 (MQ=60)
                                                                                                                   GTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTT                                >  SRR19973078.2654014/1‑150 (MQ=60)
                                                                                                                   GTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTT                                >  SRR19973078.121549/1‑150 (MQ=60)
                                                                                                                    TACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTC                               <  SRR19973078.967207/150‑1 (MQ=60)
                                                                                                                     ACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATtggaggtcagcagatc                              >  SRR19973078.1680189/1‑134 (MQ=60)
                                                                                                                      CTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAA                             >  SRR19973078.2504323/1‑150 (MQ=60)
                                                                                                                      CTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTAAA                             >  SRR19973078.1193766/1‑150 (MQ=60)
                                                                                                                            CAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGC                       >  SRR19973078.3860036/1‑150 (MQ=60)
                                                                                                                            CAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGC                       >  SRR19973078.2862040/1‑150 (MQ=60)
                                                                                                                            CAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGC                       >  SRR19973078.2862006/1‑150 (MQ=60)
                                                                                                                            CAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGC                       >  SRR19973078.3860850/1‑150 (MQ=60)
                                                                                                                             AAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTTCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTAAACGATGCC                      >  SRR19973078.421451/1‑150 (MQ=60)
                                                                                                                              AATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCA                     <  SRR19973078.4436845/150‑1 (MQ=60)
                                                                                                                                  ACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCG                 >  SRR19973078.2584391/1‑150 (MQ=60)
                                                                                                                                  ACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCG                 <  SRR19973078.2305136/150‑1 (MQ=60)
                                                                                                                                   CTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGG                >  SRR19973078.3554244/1‑150 (MQ=60)
                                                                                                                                    TTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGT               >  SRR19973078.747113/1‑150 (MQ=60)
                                                                                                                                      TATAGTTTCGCTGTCAGACTTGAATAAACGGACTTTTTTACCGTATTATAATCTAAAGCCTACACTGTCATCCTTGCCGGTTGACGAATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGATTCTTTTTCAACGATGCCACCCGGTTG             <  SRR19973078.1225994/150‑1 (MQ=60)
                                                                                                                                      GATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTG             >  SRR19973078.4177651/1‑150 (MQ=60)
                                                                                                                                       ATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGG            >  SRR19973078.3461126/1‑150 (MQ=60)
                                                                                                                                            TTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAG       >  SRR19973078.2150971/1‑150 (MQ=60)
                                                                                                                                            TTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAG       >  SRR19973078.1872364/1‑150 (MQ=60)
                                                                                                                                            TTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAG       >  SRR19973078.4292023/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG      >  SRR19973078.471642/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG      >  SRR19973078.471698/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG      >  SRR19973078.315640/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG      >  SRR19973078.804766/1‑150 (MQ=60)
                                                                                                                                             TCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGG      >  SRR19973078.3204414/1‑150 (MQ=60)
                                                                                                                                                 TGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  <  SRR19973078.4577499/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440684‑3440978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑