Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,779,324 C→A 17.5% F235L (TTC→TTA)  gpmM → phosphoglycero mutase III, cofactor‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,779,3240CA17.5% 709.4 / 73.8 255F235L (TTC→TTA) gpmMphosphoglycero mutase III, cofactor‑independent
Reads supporting (aligned to +/- strand):  ref base C (86/120);  new base A (23/22);  total (109/146)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

TCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779177‑3779472
                                                                                                                                                   |                                                                                                                                                    
  gTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGa                                                                                                                                                  <  1:1416326/150‑1 (MQ=255)
   ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGaa                                                                                                                                                 <  2:4035547/150‑1 (MQ=255)
      ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGc                                                                                                                                              <  2:4203775/150‑1 (MQ=255)
      ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGc                                                                                                                                              <  2:4203800/150‑1 (MQ=255)
      ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGc                                                                                                                                              <  1:1889710/150‑1 (MQ=255)
       tacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCg                                                                                                                                             >  2:3649845/1‑150 (MQ=255)
         cgcgATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGAc                                                                                                                                           <  1:3536120/150‑1 (MQ=255)
             aTGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGtt                                                                                                                                       >  2:2864030/1‑150 (MQ=255)
              tGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTa                                                                                                                                      <  2:2610519/150‑1 (MQ=255)
               ggACCGGTATAACGGTGGGTTTCGCGTAGAAAAAGCTTTTGACCTGCTGACTGTGGCGCAGGGCGAGTTCCAGGCCGTTACCGCCGTTGCTGGTTTGCAGGCTGCTTTTGCTCGCGACTAAATTGTTGTATTTGTGAACGCGACCGTTAt                                                                                                                                     <  2:1224043/150‑1 (MQ=255)
               ggACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTAt                                                                                                                                     <  1:346420/150‑1 (MQ=255)
               ggACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTAt                                                                                                                                     >  1:612746/1‑150 (MQ=255)
                 aCCGCGATAACCGTTGGGATCGCTTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATcc                                                                                                                                   <  2:1799489/150‑1 (MQ=255)
                  ccGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCg                                                                                                                                  <  1:1106283/150‑1 (MQ=255)
                   cgcgATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGt                                                                                                                                 <  1:753763/150‑1 (MQ=255)
                      gATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTTTGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCt                                                                                                                              <  1:1651310/150‑1 (MQ=255)
                      gATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCt                                                                                                                              >  1:3953284/1‑150 (MQ=255)
                       aTAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTg                                                                                                                             <  2:1433803/150‑1 (MQ=255)
                          aCCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCCGGCCTATACCGCCGTTGCTGGTTTGCCGGCTGCTTATGCTCGCGACGAAATTTATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAg                                                                                                                          <  2:58433/150‑1 (MQ=255)
                            cGTTGGGATCGCGTAGAAAACGCTTAGGACCTGCTGACTCGGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGTTGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGt                                                                                                                        <  1:4524419/150‑1 (MQ=255)
                             tttGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTc                                                                                                                       <  1:1121112/149‑1 (MQ=255)
                             gTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTc                                                                                                                       >  2:3619615/1‑150 (MQ=255)
                             gTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTc                                                                                                                       >  1:3423857/1‑150 (MQ=255)
                               tGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAg                                                                                                                     >  2:4620848/1‑150 (MQ=255)
                               tGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAg                                                                                                                     >  2:2041162/1‑150 (MQ=255)
                                gggATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGc                                                                                                                    <  2:4409077/150‑1 (MQ=255)
                                  gATCGCTTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAACGCGACCGTTATCCGTGCTGAAGGTCAGCCa                                                                                                                  <  1:3994489/150‑1 (MQ=255)
                                  gATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCa                                                                                                                  >  1:3940495/1‑150 (MQ=255)
                                   aTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAg                                                                                                                 >  2:4365632/1‑150 (MQ=255)
                                   aTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAg                                                                                                                 >  2:3248159/1‑150 (MQ=255)
                                   aTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAg                                                                                                                 >  2:2037989/1‑150 (MQ=255)
                                        gTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCGGCCAGAcgcg                                                                                                            <  1:3037456/150‑1 (MQ=255)
                                         tAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCgg                                                                                                           >  1:937234/1‑150 (MQ=255)
                                            aaaaaGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCaa                                                                                                        <  2:3785393/150‑1 (MQ=255)
                                             aaaaGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAAt                                                                                                       >  1:1009476/1‑150 (MQ=255)
                                               aaGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATgg                                                                                                     >  1:1244651/1‑150 (MQ=255)
                                                 gCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGaa                                                                                                   >  1:2259433/1‑150 (MQ=255)
                                                     aTGACCTGCTGACTCTGGCGCCGGGCGAGTTCCCGGCCTATACCGCCGTTGCTGGTTTGCAGGCTGATTATGCTCGCGACTAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACg                                                                                               <  1:4446241/150‑1 (MQ=255)
                                                      tGACCTGCTGACTCGGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACTAAAATGATGAATTCGTTAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACgg                                                                                              <  1:1967048/150‑1 (MQ=255)
                                                       gACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGc                                                                                             <  2:4541401/150‑1 (MQ=255)
                                                       gACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGc                                                                                             <  2:1401851/150‑1 (MQ=255)
                                                       gACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGACAATGGAAGACGGc                                                                                             <  2:485610/150‑1 (MQ=255)
                                                        aCCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCg                                                                                            <  1:3822703/150‑1 (MQ=255)
                                                        aCCTGCTGACTCTGGCGCAGAGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCg                                                                                            >  2:1577078/1‑150 (MQ=255)
                                                         ccTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGa                                                                                           >  1:1326428/1‑150 (MQ=255)
                                                         ccTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTAACCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGa                                                                                           >  1:871251/1‑150 (MQ=255)
                                                          ctgctgACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGAt                                                                                          <  1:549849/150‑1 (MQ=255)
                                                            gctgACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGc                                                                                        >  2:733637/1‑150 (MQ=255)
                                                            gctgACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGc                                                                                        >  2:733767/1‑150 (MQ=255)
                                                                 ctctGGCGCAGGGCGTTTCCCTGGCCGTTACGGCCGTTGCTGGTTTGCAGGCTGCTTATGCTGGCGACGAAAATGATGAATTCTTTAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGa                                                                                   <  1:3150951/150‑1 (MQ=255)
                                                                  tctGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAt                                                                                  >  2:1094820/1‑150 (MQ=255)
                                                                      gcgcAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTc                                                                              <  1:4590744/150‑1 (MQ=255)
                                                                        gcAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCAt                                                                            >  1:326484/1‑150 (MQ=255)
                                                                        gcAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCAt                                                                            >  2:1439904/1‑150 (MQ=255)
                                                                         cAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATg                                                                           >  2:2435578/1‑150 (MQ=255)
                                                                         cAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATg                                                                           >  1:1579428/1‑150 (MQ=255)
                                                                          aGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGa                                                                          <  2:2369447/150‑1 (MQ=255)
                                                                          aGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATACGTGCAGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGa                                                                          >  2:4600723/1‑150 (MQ=255)
                                                                          aGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGa                                                                          >  1:2821549/1‑150 (MQ=255)
                                                                             gCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACt                                                                       <  1:260644/150‑1 (MQ=255)
                                                                               gAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGATGGCGATGCACTGATTTTCATGAACTTc                                                                     <  2:3104584/150‑1 (MQ=255)
                                                                                 gTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   >  2:3446272/1‑150 (MQ=255)
                                                                                    ccAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAATGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGc                                                                >  1:2408660/1‑150 (MQ=255)
                                                                                        gCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAc                                                            <  2:3362201/150‑1 (MQ=255)
                                                                                         ccGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAcc                                                           <  2:1821049/150‑1 (MQ=255)
                                                                                           gATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAACGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc                                                         <  2:28690/150‑1 (MQ=255)
                                                                                                    gTTGCTGGTTTGCCGGGTGCTTATGCTCGCGACGAAAATGATTAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaa                                                <  1:75656/150‑1 (MQ=255)
                                                                                                         tGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCAc                                           >  1:1159725/1‑150 (MQ=255)
                                                                                                           gTTTGCAGGCTGCTTATGCTCGCGACGAAATTTATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTc                                         <  1:3313383/150‑1 (MQ=255)
                                                                                                           gTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTc                                         >  2:3957696/1‑150 (MQ=255)
                                                                                                            tttGCAGGCTGCTTATGCTCGCGACGAAAATGATGATTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCg                                        <  2:606759/150‑1 (MQ=255)
                                                                                                            tttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCg                                        <  2:2259433/150‑1 (MQ=255)
                                                                                                             ttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGt                                       >  1:1806033/1‑150 (MQ=255)
                                                                                                               gCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGc                                     <  2:3211688/150‑1 (MQ=255)
                                                                                                                cAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCt                                    <  2:1342694/150‑1 (MQ=255)
                                                                                                                cAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCt                                    <  2:1037051/150‑1 (MQ=255)
                                                                                                                 aGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCtt                                   >  2:56200/1‑150 (MQ=255)
                                                                                                                  ggCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCttt                                  <  1:3383593/150‑1 (MQ=255)
                                                                                                                   gctgctTTTGCTCGCGACGAAATTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTc                                 <  1:4300968/150‑1 (MQ=255)
                                                                                                                        ttAGGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGaa                            <  1:2150991/150‑1 (MQ=255)
                                                                                                                          aTGCTCGCGACGAAAATGATGTATTCGTTAACGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGATGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACg                          <  2:1675541/150‑1 (MQ=255)
                                                                                                                          aTGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACg                          >  2:804900/1‑150 (MQ=255)
                                                                                                                            gCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCt                        <  1:3702505/150‑1 (MQ=255)
                                                                                                                             cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTg                       <  1:3217058/150‑1 (MQ=255)
                                                                                                                             cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTg                       >  2:1694082/1‑150 (MQ=255)
                                                                                                                                   acgaAAATGATTAATCCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCg                 <  1:855830/150‑1 (MQ=255)
                                                                                                                                   acgaAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGTACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCg                 >  1:1948610/1‑150 (MQ=255)
                                                                                                                                   acgaAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCg                 <  2:1275460/150‑1 (MQ=255)
                                                                                                                                    cgaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGa                >  1:2513937/1‑150 (MQ=255)
                                                                                                                                       aaaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATgg             <  1:733637/150‑1 (MQ=255)
                                                                                                                                        aaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGc            >  2:2092232/1‑150 (MQ=255)
                                                                                                                                         atgatgAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCt           >  2:2595554/1‑150 (MQ=255)
                                                                                                                                          tgatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  1:4399871/150‑1 (MQ=255)
                                                                                                                                          tgatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  2:3844305/150‑1 (MQ=255)
                                                                                                                                           gatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTc         <  1:3849333/150‑1 (MQ=255)
                                                                                                                                           gatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTc         <  2:1326428/150‑1 (MQ=255)
                                                                                                                                            atgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcg        >  1:2751111/1‑150 (MQ=255)
                                                                                                                                            atgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcg        >  2:2798616/1‑150 (MQ=255)
                                                                                                                                             tgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgc       >  2:136920/1‑150 (MQ=255)
                                                                                                                                                  tCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTa  <  1:4620848/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779177‑3779472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A