Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,779,324 C→A 42.9% F235L (TTC→TTA)  gpmM → phosphoglycero mutase III, cofactor‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,779,3240CA42.9% 224.8 / 272.8 263F235L (TTC→TTA) gpmMphosphoglycero mutase III, cofactor‑independent
Reads supporting (aligned to +/- strand):  ref base C (62/86);  new base A (68/44);  total (130/133)
Fisher's exact test for biased strand distribution p-value = 3.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 265 total aligned reads displayed.

GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779175‑3779472
                                                                                                                                                     |                                                                                                                                                    
  tCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGt                                                                                                                                                    <  1:3628445/150‑1 (MQ=255)
    gTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGa                                                                                                                                                  <  1:1282485/150‑1 (MQ=255)
     ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGaa                                                                                                                                                 >  2:829403/1‑150 (MQ=255)
     ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGaa                                                                                                                                                 <  1:3660942/150‑1 (MQ=255)
        ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGc                                                                                                                                              <  1:1285776/150‑1 (MQ=255)
         tacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCg                                                                                                                                             <  2:662483/150‑1 (MQ=255)
            gcgATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGAcc                                                                                                                                          <  2:2888265/150‑1 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGt                                                                                                                                        >  2:3213130/1‑150 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGt                                                                                                                                        >  1:3646437/1‑150 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGt                                                                                                                                        >  2:1829249/1‑150 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGt                                                                                                                                        >  1:251168/1‑150 (MQ=255)
                tGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTa                                                                                                                                      >  1:1361582/1‑150 (MQ=255)
                 ggACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTAt                                                                                                                                     >  2:3594299/1‑150 (MQ=255)
                  gACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATc                                                                                                                                    >  1:3302481/1‑150 (MQ=255)
                  gACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATc                                                                                                                                    >  2:1455077/1‑150 (MQ=255)
                   aCCGCTATAACCGTTGGTATCGCGTATAAAAAGTTTATGACTTGCTGACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCGGTTGCTGGTTTGCAGGCTGCTTTTGATCGCTAATAAAATTTTTAATTAGTGAAAGCGACCGTTATcc                                                                                                                                   <  2:1740278/150‑1 (MQ=255)
                   aCCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATcc                                                                                                                                   <  2:3314915/150‑1 (MQ=255)
                    ccGCGATAACGGTTGGGATCGCGTAGAAAAAGCTTATGACGTGCTTACTCTGGCGCAGGGCGTGTTCCAGGCCGATACCGCCGTTGGTGGTTTGCTGGCTGCTTATGCTCGCGACGAAATTGATTAATTCGTGAACGCGACCGTTATCCg                                                                                                                                  <  1:3913260/150‑1 (MQ=255)
                        gATAACCGTTGGGATCGCGTAGAAAAAGCTTTTGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATTAATTAGTGAAAGCGACCGTTATCCGTGCt                                                                                                                              <  1:1002299/150‑1 (MQ=255)
                        gATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCt                                                                                                                              >  1:1514824/1‑150 (MQ=255)
                          tAACCGTTGGGATCGCGAAGAAAAAGCTTAATACCTGCAGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGTTTTGCAGGCTGCTTATGCTAGCGACGAAAAAGAAGAATTCGTGAAAGCGACAGTTATCCGtgatga                                                                                                                            >  2:1982279/1‑150 (MQ=255)
                           aaCCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGaa                                                                                                                           >  1:2170639/1‑150 (MQ=255)
                            aCCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCTGGCCGATCCCGCCGTTGCTGGTTTGCAGGCTTCTTTTGCTCGCGACTAAAATGATTAATTAGTGAAAGCGACCGTTATCCGTGCTGAAg                                                                                                                          <  1:1233013/150‑1 (MQ=255)
                             ccGTTGGGATCGCGTAGAAAAAGCTTATCACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATTATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAgg                                                                                                                         <  2:424953/150‑1 (MQ=255)
                              cGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGt                                                                                                                        >  1:1975488/1‑150 (MQ=255)
                                ttGGGATCGCGTAGAAAACGCTTATGACCTGCTGACTCTGGCGCCGGGCGTGTTCCCGGCCGATCCCGCCGTTGCTGGTTTGCTGGCTGCTTAGGCTCGCGACGAAAATGATTAATTAGTGAACGCGACCGTTATCCGTGCTGAAGGTCa                                                                                                                      <  1:3494110/150‑1 (MQ=255)
                                ttGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCa                                                                                                                      >  1:2751562/1‑150 (MQ=255)
                                ttGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCa                                                                                                                      >  2:4094259/1‑150 (MQ=255)
                                     aTCGCGTAGAAAAAGCTTATTACCTGCTGACTCTGGCGCTGGGCGTGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATTAATTAGTTAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAg                                                                                                                 <  1:161113/150‑1 (MQ=255)
                                      tCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGa                                                                                                                >  2:890607/1‑150 (MQ=255)
                                       cgcgTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAc                                                                                                               >  2:3132889/1‑150 (MQ=255)
                                          gTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAcgcg                                                                                                            >  1:203040/1‑150 (MQ=255)
                                           tAGAAAAAGCTTCTGACCTGCTGACTCTGGCGCAGGGCGTGTTCCAGGCCGATACCGCGGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGTTGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCgg                                                                                                           <  1:161769/150‑1 (MQ=255)
                                            aGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGc                                                                                                          >  1:2892974/1‑150 (MQ=255)
                                               aaaaGCTTATCACCGGCTGACTCGGGCGCAGGGCGAGTTCCAGGCCGATCCCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACTAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAAt                                                                                                       <  1:118049/150‑1 (MQ=255)
                                                  aGCTTATTACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGa                                                                                                    <  2:685169/150‑1 (MQ=255)
                                                  aGCTTATGACCTGCTGACTCTGGCGCAGGGCGTGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGa                                                                                                    <  1:1038731/150‑1 (MQ=255)
                                                   gCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGaa                                                                                                   <  2:3915345/150‑1 (MQ=255)
                                                    cTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAg                                                                                                  <  2:3739077/150‑1 (MQ=255)
                                                      tATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGAc                                                                                                <  2:3692124/150‑1 (MQ=255)
                                                      tATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGAc                                                                                                <  2:1846702/150‑1 (MQ=255)
                                                       aTGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACg                                                                                               <  2:3397457/150‑1 (MQ=255)
                                                        tGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGGTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAAGGGAGGACgg                                                                                              >  2:735851/1‑150 (MQ=255)
                                                        tGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTGGCTGGTTTGCCGGCTGCTTATGCTCGCGACGAAAATGATTAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACgg                                                                                              <  1:2824476/150‑1 (MQ=255)
                                                          aCCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCg                                                                                            >  1:910668/1‑150 (MQ=255)
                                                           ccTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATTATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGa                                                                                           <  2:3865190/150‑1 (MQ=255)
                                                             tgctgACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATg                                                                                         >  2:927058/1‑150 (MQ=255)
                                                                 gACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACt                                                                                     <  2:1002662/150‑1 (MQ=255)
                                                                 gACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCCCt                                                                                     >  2:2917347/1‑150 (MQ=255)
                                                                   ctcgGGCGCTGGGCGTGTTCCCGGCCTATACCGCCGTTGCTGGTTTTGAGGCTGCTTTTGCTCGGTACTAACATGTTTAATTGGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGa                                                                                   <  1:3166924/150‑1 (MQ=255)
                                                                      tGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAttt                                                                                >  1:238458/1‑150 (MQ=255)
                                                                        gcgcAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCGGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTc                                                                              <  2:1942454/150‑1 (MQ=255)
                                                                          ggaGGGCTTGTTCCCGGGGGATAGCGGGGTTGCTGGTTTGCAGTGTGCTTTTGGTCGCTAGGAATATGTTGAATTTGTGATAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCAt                                                                            <  1:3678210/148‑1 (MQ=255)
                                                                           cAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATg                                                                           <  1:2533397/150‑1 (MQ=255)
                                                                            aGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGTTTAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGa                                                                          <  1:1898069/150‑1 (MQ=255)
                                                                            aGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCACTGGCAGACGGCGATGCACTGATTTTCATGa                                                                          >  2:3246727/1‑150 (MQ=255)
                                                                                 gAGTTCCTGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTTGGCTCGCTACTAAAATGATGATTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTc                                                                     <  1:2542974/150‑1 (MQ=255)
                                                                                 gAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTc                                                                     <  1:1607853/150‑1 (MQ=255)
                                                                                  tgTTCCAGGCCTATACCGCCGTTGCTGGTTTGCAGGCTGCTTTTGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTcc                                                                    <  1:3947186/149‑1 (MQ=255)
                                                                                  aGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGATTATGCTCGCGACTAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTcc                                                                    <  2:1228647/150‑1 (MQ=255)
                                                                                   gTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   >  2:2766195/1‑150 (MQ=255)
                                                                                       ccggCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTTAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCt                                                               <  1:755524/148‑1 (MQ=255)
                                                                                       cAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCCATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCt                                                               >  1:2670305/1‑150 (MQ=255)
                                                                                         ggCCGATACCGCCGTTGCGGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGa                                                             >  1:2098906/1‑150 (MQ=255)
                                                                                          gCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAACTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAc                                                            <  1:386101/150‑1 (MQ=255)
                                                                                            cGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGGTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcg                                                          >  1:1138248/1‑150 (MQ=255)
                                                                                               tACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcgc                                                       >  2:1901736/1‑150 (MQ=255)
                                                                                                aCCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCCGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcgcg                                                      >  2:3610077/1‑150 (MQ=255)
                                                                                                     cgTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGa                                                 >  2:2822811/1‑150 (MQ=255)
                                                                                                      gTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaa                                                >  1:3595661/1‑150 (MQ=255)
                                                                                                      gTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaa                                                >  1:3762048/1‑150 (MQ=255)
                                                                                                      gTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaa                                                >  1:3762012/1‑150 (MQ=255)
                                                                                                       ttGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaaa                                               >  2:2948466/1‑150 (MQ=255)
                                                                                                        tGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAAt                                              >  2:2270004/1‑150 (MQ=255)
                                                                                                         gCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATc                                             >  1:844828/1‑150 (MQ=255)
                                                                                                             gTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTc                                         >  1:2834111/1‑150 (MQ=255)
                                                                                                             gTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTc                                         <  2:1421396/150‑1 (MQ=255)
                                                                                                               ttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGt                                       <  2:2641277/150‑1 (MQ=255)
                                                                                                                tGCTGGCTGCTTATGCTCGCGACGAAAATGTTGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTg                                      <  1:1218899/150‑1 (MQ=255)
                                                                                                                   aGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCtt                                   >  2:2171476/1‑150 (MQ=255)
                                                                                                                   aGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCtt                                   <  1:4151622/150‑1 (MQ=255)
                                                                                                                     gctgctTATGCTCGCGACGAAAATTATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTc                                 <  1:3279815/150‑1 (MQ=255)
                                                                                                                              gCTCGCGACGAAAATGATTAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCt                        <  1:1740486/150‑1 (MQ=255)
                                                                                                                              gCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCt                        <  1:2958124/150‑1 (MQ=255)
                                                                                                                              gCTCGCGACGAAAATGATGAATTAGTGAACGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCt                        <  1:561437/150‑1 (MQ=255)
                                                                                                                               cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTg                       <  2:1306594/150‑1 (MQ=255)
                                                                                                                                 cgcgACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGAt                     <  2:2040924/150‑1 (MQ=255)
                                                                                                                                  gcgACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGAtt                    <  1:3783754/150‑1 (MQ=255)
                                                                                                                                   cgacgaAAATGATGCATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGAttt                   >  2:1188102/1‑150 (MQ=255)
                                                                                                                                    gacgaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTc                  <  2:1613077/150‑1 (MQ=255)
                                                                                                                                       gaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGAt               <  2:2170639/150‑1 (MQ=255)
                                                                                                                                         aaaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATgg             >  2:2334663/1‑150 (MQ=255)
                                                                                                                                           atgatgAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCt           <  2:1072066/150‑1 (MQ=255)
                                                                                                                                            tgatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  2:349444/150‑1 (MQ=255)
                                                                                                                                                gaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcg      >  1:3806284/1‑150 (MQ=255)
                                                                                                                                                 aaTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgc     >  1:1116301/1‑150 (MQ=255)
                                                                                                                                                 aaTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCAAGACGAGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGAGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTagcgc     >  2:1541720/1‑150 (MQ=255)
                                                                                                                                                 aaTTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgc     <  2:251168/150‑1 (MQ=255)
                                                                                                                                                 aaTTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgc     <  2:3646420/150‑1 (MQ=255)
                                                                                                                                                  aTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgcg    >  1:2754192/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779175‑3779472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A