Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,779,324 C→A 42.8% F235L (TTC→TTA)  gpmM → phosphoglycero mutase III, cofactor‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,779,3240CA42.8% 271.0 / 263.5 280F235L (TTC→TTA) gpmMphosphoglycero mutase III, cofactor‑independent
Reads supporting (aligned to +/- strand):  ref base C (70/87);  new base A (55/65);  total (125/155)
Fisher's exact test for biased strand distribution p-value = 9.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779175‑3779472
                                                                                                                                                     |                                                                                                                                                    
  tCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGt                                                                                                                                                    <  2:1285725/150‑1 (MQ=255)
    gTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGa                                                                                                                                                  <  2:930337/150‑1 (MQ=255)
    gTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGa                                                                                                                                                  >  1:3026647/1‑150 (MQ=255)
     ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGaa                                                                                                                                                 <  2:4482546/150‑1 (MQ=255)
      tactacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGaaa                                                                                                                                                <  2:497023/150‑1 (MQ=255)
       actacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAg                                                                                                                                               <  2:4197869/150‑1 (MQ=255)
       actacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAg                                                                                                                                               <  2:4197997/150‑1 (MQ=255)
        ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGc                                                                                                                                              <  2:1155734/150‑1 (MQ=255)
        ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGc                                                                                                                                              <  1:3022081/150‑1 (MQ=255)
          acGCGATGGACCTCGATAACCGTTGTGATCGCTTAGAAAAAGCTTATGACCTGCTGACTCTGTCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTTCTGGTTTGCAGGCTGCTTATGCTCGCGACTAAAATTATGAATTCGTGAAAGCGa                                                                                                                                            <  2:1086628/150‑1 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGt                                                                                                                                        >  2:3951478/1‑150 (MQ=255)
              gATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGt                                                                                                                                        >  1:2365428/1‑150 (MQ=255)
                tGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTa                                                                                                                                      <  2:2831564/150‑1 (MQ=255)
                gggACCGCGATAACCGTTGGGATCGCTTAGAAAAAGCTTATGACCTGCTTACTCTGGCGCAGGGCGTGTTCCAGGCCGATACCGCGGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACTAAAATGATGAATTCGTGAAAGCGACCGTTa                                                                                                                                      <  1:486424/149‑1 (MQ=255)
                 ggACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGATTTTGATGAATTCGTGAAAGCGACCGTTAt                                                                                                                                     <  2:3393561/150‑1 (MQ=255)
                   aCCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATcc                                                                                                                                   <  2:2248399/150‑1 (MQ=255)
                    ccGCGATAACCGTTGGGATCGCGTATAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCg                                                                                                                                  <  2:3566151/150‑1 (MQ=255)
                     cgcgATAACCGTTGGGATCGCGTATAAAAAGCTTATGACCTGCTGACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCGGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACTAAATTGATTAATTAGTGAAAGCGACCGTTATCCGt                                                                                                                                 <  1:4158505/150‑1 (MQ=255)
                     cgcgATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGt                                                                                                                                 >  1:1106057/1‑150 (MQ=255)
                          tAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGa                                                                                                                            >  1:2640899/1‑150 (MQ=255)
                              cGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGt                                                                                                                        >  1:2482456/1‑150 (MQ=255)
                               gTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTc                                                                                                                       >  2:2318967/1‑150 (MQ=255)
                                 tGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCCGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAg                                                                                                                     <  1:241227/150‑1 (MQ=255)
                                  ggtagcgctTAGAAAATGCTTATGACGTGCTGACTCTGGCGCTGGGCGTGTTCCAGGCCTATACCGCCGTTGCTGGTTTGCCGGCTGCTTATGCTCGCGACTAAATTGATTAATTCGTTAAAGCGACCGTTATCCGTGCTGAAGGTCAGc                                                                                                                    <  1:2049758/145‑1 (MQ=255)
                                    gATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGTGTTCCAGGCCGATACCGCCGTTGCTGGTGTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCa                                                                                                                  <  1:411415/150‑1 (MQ=255)
                                    gATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCa                                                                                                                  >  2:184102/1‑150 (MQ=255)
                                       cgcgTAGAAAAAGCTTAGGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCTTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATTAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAc                                                                                                               <  2:4294896/150‑1 (MQ=255)
                                          gTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAcgcg                                                                                                            <  1:2237866/150‑1 (MQ=255)
                                          gTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAcgcg                                                                                                            >  1:2337097/1‑150 (MQ=255)
                                              aaaaaGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCaa                                                                                                        <  2:3996964/150‑1 (MQ=255)
                                               aaaaGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAAt                                                                                                       >  2:439781/1‑150 (MQ=255)
                                                  aGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGa                                                                                                    <  2:4502779/150‑1 (MQ=255)
                                                     ttATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCCGACGCGGCAATGGAAGa                                                                                                 >  2:203665/1‑150 (MQ=255)
                                                      tATGACCTGCTGACTCTGGCGCTGGGCGAGTTCCCGGCCTATACCGCCGTTGCTGGTTTGCAGGCTGATTATGCTCGCGACTAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGAc                                                                                                <  1:828640/150‑1 (MQ=255)
                                                      tATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGAc                                                                                                >  1:3432527/1‑150 (MQ=255)
                                                       aTGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACg                                                                                               <  1:3571031/150‑1 (MQ=255)
                                                       aTGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACg                                                                                               <  2:2952283/150‑1 (MQ=255)
                                                        tGACCTGCTGACTCTGGCGCTGGGCGAGTTCCTGGCCGATACCGCCGTTGCGGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACgg                                                                                              <  1:4039664/150‑1 (MQ=255)
                                                         caCCTGCTGACTCTGGCGCAGGGCGAGTTCCCGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATTATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGc                                                                                             <  1:2866660/149‑1 (MQ=255)
                                                          aCCTGCTGACTCTGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGATTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCg                                                                                            <  1:3721711/150‑1 (MQ=255)
                                                           ccTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGa                                                                                           <  2:3477197/150‑1 (MQ=255)
                                                           ccTGCTGACTCGGGCGCTGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTTTGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGa                                                                                           <  1:1962739/150‑1 (MQ=255)
                                                            ctgctgACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAATTGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGAt                                                                                          <  1:135757/150‑1 (MQ=255)
                                                               ctgACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCa                                                                                       >  2:1645112/1‑150 (MQ=255)
                                                                 gACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACt                                                                                     >  2:2133866/1‑150 (MQ=255)
                                                                      tGGCGCTGGGCGTGTTCCTGGCCGATACCGCGGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACTAAAATTATGAATTAGTGAACGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAttt                                                                                <  1:1218860/150‑1 (MQ=255)
                                                                          gcAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCAt                                                                            <  2:525323/150‑1 (MQ=255)
                                                                          gcAGGGCGAGTTCCAGGCCGATACCGCCGTGGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCAt                                                                            <  1:952535/150‑1 (MQ=255)
                                                                           cAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATg                                                                           >  2:3478172/1‑150 (MQ=255)
                                                                             gggCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGaa                                                                         <  2:2672773/150‑1 (MQ=255)
                                                                             gggCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGaa                                                                         <  2:3244448/150‑1 (MQ=255)
                                                                                 gAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTAAGGCTCGCGACGAAAATGATTAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTc                                                                     <  2:788974/150‑1 (MQ=255)
                                                                                   gtgccAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   <  2:4466826/147‑1 (MQ=255)
                                                                                   gTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   <  1:3330214/150‑1 (MQ=255)
                                                                                   gTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   >  2:3911881/1‑150 (MQ=255)
                                                                                   gTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                                                                   <  2:4547277/150‑1 (MQ=255)
                                                                                      ccAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGc                                                                <  2:2521410/150‑1 (MQ=255)
                                                                                      ccAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAAAGGAAGACGGCGATGCACTGATTTTAATGAACTTCCGTGc                                                                >  1:3895149/1‑150 (MQ=255)
                                                                                       cAGGCCGATACCGCCGTTGCTGTTTTGCAGGCTGCTTATGCTCGCGACTAAAATTATGAATTCGTGAAAGCGACCTTTATCCGTGCTGAATGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCt                                                               <  2:659193/150‑1 (MQ=255)
                                                                                       cAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTAGCGACGAAAAAGATGAAATCGTGAAAGCGACCCTTAACCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGAAGCACAGATTTTCATGAACTTCCGTGCt                                                               >  2:3197040/1‑150 (MQ=255)
                                                                                        aGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTg                                                              >  1:1713427/1‑150 (MQ=255)
                                                                                        aGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTg                                                              <  2:1516505/150‑1 (MQ=255)
                                                                                          gCCGTTACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATTAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAc                                                            <  2:3180923/150‑1 (MQ=255)
                                                                                          gCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAc                                                            <  2:3180380/150‑1 (MQ=255)
                                                                                           ccGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAcc                                                           <  1:3471846/150‑1 (MQ=255)
                                                                                           ccGATACCGCCGTTGCTGGGTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAcc                                                           >  2:523099/1‑150 (MQ=255)
                                                                                             gATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc                                                         >  2:196443/1‑150 (MQ=255)
                                                                                                     cgTTGCTGGTTTGCTGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGa                                                 <  1:2883357/150‑1 (MQ=255)
                                                                                                     cgTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGa                                                 >  2:242086/1‑150 (MQ=255)
                                                                                                       ttGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGaaa                                               <  1:1802898/150‑1 (MQ=255)
                                                                                                         gCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATc                                             <  2:1022305/150‑1 (MQ=255)
                                                                                                           tGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCAc                                           >  2:756170/1‑150 (MQ=255)
                                                                                                            ggTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACt                                          >  1:83985/1‑150 (MQ=255)
                                                                                                              tttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCg                                        <  1:2192414/150‑1 (MQ=255)
                                                                                                               ttGCTGGCTGATTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGt                                       <  1:2033105/150‑1 (MQ=255)
                                                                                                               ttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGt                                       <  2:100054/150‑1 (MQ=255)
                                                                                                                 gCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGc                                     >  2:1440169/1‑150 (MQ=255)
                                                                                                                  cAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCt                                    >  1:3169995/1‑150 (MQ=255)
                                                                                                                    ggCTGCTTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCttt                                  >  1:3618954/1‑150 (MQ=255)
                                                                                                                       tgctTATGCTCGCGACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGt                               >  1:3188491/1‑150 (MQ=255)
                                                                                                                         ctTATGCTCGCGACGAAAATGATGAAGTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGa                             >  2:2089567/1‑150 (MQ=255)
                                                                                                                               cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGTCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTg                       >  1:2693271/1‑150 (MQ=255)
                                                                                                                                 cgcgACGAAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGAt                     >  1:1124751/1‑150 (MQ=255)
                                                                                                                                    gacgaAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTc                  <  1:2262993/150‑1 (MQ=255)
                                                                                                                                      cgaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGa                <  2:3054594/150‑1 (MQ=255)
                                                                                                                                       gaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGAt               >  1:4483937/1‑150 (MQ=255)
                                                                                                                                       gaAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGAt               >  1:4326636/1‑150 (MQ=255)
                                                                                                                                       gaAAATGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGAt               <  2:1806667/150‑1 (MQ=255)
                                                                                                                                        aaaaTTATGAATTAGTTAAAGCGACAGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATg              <  2:1555176/150‑1 (MQ=255)
                                                                                                                                        aaaaTGATGAATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATg              >  1:750588/1‑150 (MQ=255)
                                                                                                                                         aaaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATgg             >  1:3604228/1‑150 (MQ=255)
                                                                                                                                         aaaTGATGAATTATTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGTAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATgg             <  2:2098962/150‑1 (MQ=255)
                                                                                                                                            tgatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  1:2009625/150‑1 (MQ=255)
                                                                                                                                            tgatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  1:1921799/150‑1 (MQ=255)
                                                                                                                                            tgatgaATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCtt          <  2:846818/150‑1 (MQ=255)
                                                                                                                                             gatgaATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTc         <  1:2799040/150‑1 (MQ=255)
                                                                                                                                                gaATTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcg      <  2:2050964/150‑1 (MQ=255)
                                                                                                                                                  aTTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgcg    <  2:1753688/150‑1 (MQ=255)
                                                                                                                                                  aTTAGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTcgcgcg    <  2:1229659/150‑1 (MQ=255)
                                                                                                                                                   ttCGTGAAAGCGAACGTTATCCGTGCTGAAGGACAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGATGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGTCTTCGCGCGt   >  2:1562977/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTA  >  CP009273/3779175‑3779472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A