Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,799,108 C→A 18.0% R68L (CGG→CTG)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,799,1080CA18.0% 816.6 / 112.8 266R68L (CGG→CTG) waaPkinase that phosphorylates core heptose of lipopolysaccharide
Reads supporting (aligned to +/- strand):  ref base C (101/117);  new base A (16/32);  total (117/149)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 269 total aligned reads displayed.

AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCA  >  CP009273/3798960‑3799257
                                                                                                                                                    |                                                                                                                                                     
aGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCg                                                                                                                                                      >  2:2019232/1‑150 (MQ=255)
 gTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGc                                                                                                                                                     >  1:3528610/1‑150 (MQ=255)
 gTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGc                                                                                                                                                     <  1:3974882/150‑1 (MQ=255)
  tGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCa                                                                                                                                                    <  1:2452277/150‑1 (MQ=255)
      aTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATga                                                                                                                                                >  1:4003752/1‑150 (MQ=255)
      aTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATga                                                                                                                                                >  2:1343956/1‑150 (MQ=255)
      aTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATga                                                                                                                                                <  2:1328187/150‑1 (MQ=255)
       tCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATgag                                                                                                                                               >  1:3139510/1‑150 (MQ=255)
         ttCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGagag                                                                                                                                             <  2:136676/150‑1 (MQ=255)
          tCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGt                                                                                                                                            >  1:644667/1‑150 (MQ=255)
           cGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTa                                                                                                                                           >  1:4354206/1‑150 (MQ=255)
           cGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTa                                                                                                                                           >  1:2882724/1‑150 (MQ=255)
            ggTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAg                                                                                                                                          <  1:4223626/150‑1 (MQ=255)
                  aataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGAttttt                                                                                                                                    >  1:3212152/1‑150 (MQ=255)
                     aaaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTtat                                                                                                                                 >  2:639536/1‑150 (MQ=255)
                     aaaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTtat                                                                                                                                 <  2:2723172/150‑1 (MQ=255)
                          atgCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAAtct                                                                                                                            <  2:59295/150‑1 (MQ=255)
                           tgCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAAtctc                                                                                                                           <  2:3417332/150‑1 (MQ=255)
                           tgCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAAtctc                                                                                                                           >  1:4055312/1‑150 (MQ=255)
                            gcgcGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATctct                                                                                                                          >  1:4378659/1‑150 (MQ=255)
                              gcgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCttt                                                                                                                        <  1:2405988/150‑1 (MQ=255)
                               cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                       <  2:320302/150‑1 (MQ=255)
                               cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                       >  2:1634633/1‑150 (MQ=255)
                                gAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTa                                                                                                                      >  1:1084002/1‑150 (MQ=255)
                                  gTTAATTGATTTAACCCTTTATCGCCAAACCAAATGCCCTTCATTGTATCAAAGCAAACAACAATCAGGCGATGAATATCTTTACAATCTCTGTCTGCGCACAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATctctaataaa                                                                                                                    >  2:661308/1‑144 (MQ=255)
                                      aTGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtcg                                                                                                                >  2:300078/1‑150 (MQ=255)
                                      aTGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtcg                                                                                                                >  2:1841644/1‑150 (MQ=255)
                                       tGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtcgt                                                                                                               >  1:162609/1‑150 (MQ=255)
                                         gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                             <  1:2739243/150‑1 (MQ=255)
                                         gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                             >  1:3836571/1‑150 (MQ=255)
                                         gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                             <  1:2739187/150‑1 (MQ=255)
                                         gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                             >  1:1284137/1‑150 (MQ=255)
                                         gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                             <  1:2172871/150‑1 (MQ=255)
                                          aTTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATTAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTccc                                                                                                            >  1:990132/1‑150 (MQ=255)
                                             tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCTCTCAGGCGATGAATAGCGTGCCTCTCTATGTCTGCGACCAAAACGGGCATCCGCTATGATAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         <  2:4126238/150‑1 (MQ=255)
                                             tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         <  1:3088880/150‑1 (MQ=255)
                                             tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         >  1:935031/1‑150 (MQ=255)
                                             tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         <  2:3707662/150‑1 (MQ=255)
                                               aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgt                                                                                                       <  1:670058/150‑1 (MQ=255)
                                               aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgt                                                                                                       >  2:1817439/1‑150 (MQ=255)
                                                cccTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgtg                                                                                                      >  1:402974/1‑150 (MQ=255)
                                                    ttttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCAt                                                                                                  <  2:487376/150‑1 (MQ=255)
                                                      ttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCAttt                                                                                                <  1:1666904/150‑1 (MQ=255)
                                                       tCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTa                                                                                               <  1:2549572/150‑1 (MQ=255)
                                                       tCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTa                                                                                               <  2:2964575/150‑1 (MQ=255)
                                                         gCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAg                                                                                             >  1:4637665/1‑150 (MQ=255)
                                                         gCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAg                                                                                             >  1:2161796/1‑150 (MQ=255)
                                                          ccAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGa                                                                                            <  1:4191975/150‑1 (MQ=255)
                                                           cAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaa                                                                                           >  1:446005/1‑150 (MQ=255)
                                                           cAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaa                                                                                           >  2:1569970/1‑150 (MQ=255)
                                                                ccAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAg                                                                                      <  1:3647168/150‑1 (MQ=255)
                                                                 cAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGc                                                                                     <  1:1046447/150‑1 (MQ=255)
                                                                 cAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGc                                                                                     <  2:2129940/150‑1 (MQ=255)
                                                                  aaTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCt                                                                                    >  2:2008968/1‑150 (MQ=255)
                                                                    tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                  <  1:1545705/150‑1 (MQ=255)
                                                                    tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                  <  2:2200636/150‑1 (MQ=255)
                                                                    tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                  <  1:2624797/150‑1 (MQ=255)
                                                                    tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                  <  1:3309380/150‑1 (MQ=255)
                                                                      cccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTc                                                                                <  2:2325503/150‑1 (MQ=255)
                                                                       ccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTcc                                                                               >  2:1299729/1‑150 (MQ=255)
                                                                        cTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTccc                                                                              <  2:1766555/150‑1 (MQ=255)
                                                                           cATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGa                                                                           <  2:3772047/150‑1 (MQ=255)
                                                                             ttGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACa                                                                         <  2:3860492/150‑1 (MQ=255)
                                                                              tGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAg                                                                        >  2:1708266/1‑150 (MQ=255)
                                                                               gTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGt                                                                       >  1:1101993/1‑150 (MQ=255)
                                                                                 aTCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTc                                                                     >  1:1742806/1‑150 (MQ=255)
                                                                                    aaCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCaaa                                                                  >  1:1179204/1‑150 (MQ=255)
                                                                                       gCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCg                                                               >  1:3734720/1‑150 (MQ=255)
                                                                                          aaCATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTaa                                                            <  1:3947946/150‑1 (MQ=255)
                                                                                              tCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTa                                                        <  2:3718179/150‑1 (MQ=255)
                                                                                               cACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTac                                                       <  2:4003706/150‑1 (MQ=255)
                                                                                                aCTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTacg                                                      >  1:797949/1‑150 (MQ=255)
                                                                                                aCTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTacg                                                      <  1:4071378/150‑1 (MQ=255)
                                                                                                  tCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTacgac                                                    <  2:4417411/150‑1 (MQ=255)
                                                                                                     ggCGATGAATAGCGTGCCACTCTCTTTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAg                                                 >  1:3021442/1‑150 (MQ=255)
                                                                                                      gCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGt                                                <  2:2132501/150‑1 (MQ=255)
                                                                                                       cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               >  1:1998225/1‑150 (MQ=255)
                                                                                                       cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               >  1:441132/1‑150 (MQ=255)
                                                                                                       cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               <  1:533923/150‑1 (MQ=255)
                                                                                                               aGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTc                                       <  2:2932783/150‑1 (MQ=255)
                                                                                                               aGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTc                                       <  2:2932863/150‑1 (MQ=255)
                                                                                                                gCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCa                                      <  2:3066828/150‑1 (MQ=255)
                                                                                                                      cACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGaaat                                <  1:4616221/150‑2 (MQ=255)
                                                                                                                       aCTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGa                               <  1:1681924/150‑1 (MQ=255)
                                                                                                                        ctctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGAc                              <  1:2259004/150‑1 (MQ=255)
                                                                                                                        ctctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGAc                              <  2:4379330/150‑1 (MQ=255)
                                                                                                                         tctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGAcc                             <  2:3431877/150‑1 (MQ=255)
                                                                                                                          ctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCt                            <  2:1995440/150‑1 (MQ=255)
                                                                                                                           tctgtctgCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTc                           <  2:3026048/150‑1 (MQ=255)
                                                                                                                                     cccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGt                 >  1:902667/1‑150 (MQ=255)
                                                                                                                                     cccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGt                 >  1:902753/1‑150 (MQ=255)
                                                                                                                                      ccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtt                <  1:2063710/150‑1 (MQ=255)
                                                                                                                                       cAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt               >  1:3160982/1‑150 (MQ=255)
                                                                                                                                       cAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt               >  2:4599554/1‑150 (MQ=255)
                                                                                                                                         aaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttttt             >  2:3236573/1‑150 (MQ=255)
                                                                                                                                          aaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttttt            <  2:1059630/150‑1 (MQ=255)
                                                                                                                                           aCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTg           <  2:4067717/150‑1 (MQ=255)
                                                                                                                                            cGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGa          <  2:3348310/150‑1 (MQ=255)
                                                                                                                                                  tCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTg    <  2:869515/150‑1 (MQ=255)
                                                                                                                                                   ccGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGc   <  1:787898/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCA  >  CP009273/3798960‑3799257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A