Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,799,108 C→A 47.5% R68L (CGG→CTG)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,799,1080CA47.5% 171.6 / inf 275R68L (CGG→CTG) waaPkinase that phosphorylates core heptose of lipopolysaccharide
Reads supporting (aligned to +/- strand):  ref base C (58/86);  new base A (67/64);  total (125/150)
Fisher's exact test for biased strand distribution p-value = 8.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTG  >  CP009273/3798962‑3799255
                                                                                                                                                  |                                                                                                                                                   
tGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCa                                                                                                                                                  <  1:3825454/150‑1 (MQ=255)
    aTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATga                                                                                                                                              >  1:2838911/1‑150 (MQ=255)
     tCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATgag                                                                                                                                             <  1:776359/150‑1 (MQ=255)
      cTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATgaga                                                                                                                                            <  1:1794057/150‑1 (MQ=255)
         cGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTa                                                                                                                                         <  1:3942087/150‑1 (MQ=255)
            taataataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAt                                                                                                                                      >  1:547022/1‑150 (MQ=255)
             aataataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAtt                                                                                                                                     >  1:3107693/1‑150 (MQ=255)
              ataataaATGATGCGCGAGTTAATGGATTTAACCCTTGTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGAttt                                                                                                                                    <  2:489565/150‑1 (MQ=255)
                aataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAttttt                                                                                                                                  <  1:2751992/150‑1 (MQ=255)
                    aaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTtata                                                                                                                              >  1:1150064/1‑150 (MQ=255)
                     atgatgCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATaa                                                                                                                             <  2:2488455/150‑1 (MQ=255)
                     atgatgCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATaa                                                                                                                             <  1:2082865/150‑1 (MQ=255)
                           cgcgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCtt                                                                                                                       <  2:1444009/150‑1 (MQ=255)
                           cgcgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCtt                                                                                                                       <  2:2876892/150‑1 (MQ=255)
                             cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                     <  2:792549/150‑1 (MQ=255)
                             cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCAAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCTTCCGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                     <  1:3843438/150‑1 (MQ=255)
                               aGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTGCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTaa                                                                                                                   >  2:4563591/1‑150 (MQ=255)
                                   aaTGGATTTAACCCTTTTTCGCCAAACCCACTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtc                                                                                                               <  1:3885755/150‑1 (MQ=255)
                                   aaTGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtc                                                                                                               <  1:2986962/150‑1 (MQ=255)
                                    aTGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtcg                                                                                                              >  2:1490960/1‑150 (MQ=255)
                                       gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                           >  1:892369/1‑150 (MQ=255)
                                       gATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTcc                                                                                                           >  1:3271442/1‑150 (MQ=255)
                                          ttAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCt                                                                                                        >  1:4053168/1‑150 (MQ=255)
                                           tAACCC‑TTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtg                                                                                                      >  2:2307576/1‑150 (MQ=255)
                                            aaCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtg                                                                                                      <  2:1214596/150‑1 (MQ=255)
                                             aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgt                                                                                                     <  1:1388661/150‑1 (MQ=255)
                                               ccTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGc                                                                                                   >  2:2342412/1‑150 (MQ=255)
                                                cTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGcc                                                                                                  <  1:3557498/150‑1 (MQ=255)
                                                 tttttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCa                                                                                                 <  1:55307/150‑1 (MQ=255)
                                                 tttttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCa                                                                                                 <  1:113069/150‑1 (MQ=255)
                                                 tttttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCa                                                                                                 <  2:2209433/150‑1 (MQ=255)
                                                 tttttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCCGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCa                                                                                                 >  2:1272187/1‑150 (MQ=255)
                                                    ttCGCCAAACCCAAGGCCCTTCATTGTATCATCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCAttt                                                                                              <  1:1230647/150‑1 (MQ=255)
                                                        ccAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGa                                                                                          <  1:1838083/150‑1 (MQ=255)
                                                        ccAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGa                                                                                          >  1:2819035/1‑150 (MQ=255)
                                                         cAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaa                                                                                         >  2:4035459/1‑150 (MQ=255)
                                                          aaaCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaaa                                                                                        >  1:3707681/1‑150 (MQ=255)
                                                          aaaCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaaa                                                                                        <  2:4575353/150‑1 (MQ=255)
                                                           aaCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaaaa                                                                                       >  2:1234651/1‑150 (MQ=255)
                                                           aaCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGaaaa                                                                                       <  1:432880/150‑1 (MQ=255)
                                                            aCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAAt                                                                                      <  2:3931826/150‑1 (MQ=255)
                                                              ccAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAg                                                                                    <  2:4204291/150‑1 (MQ=255)
                                                              ccAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAg                                                                                    <  1:142074/150‑1 (MQ=255)
                                                               cAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGc                                                                                   >  2:3031826/1‑150 (MQ=255)
                                                               cAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGc                                                                                   <  2:1064813/150‑1 (MQ=255)
                                                                 aTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCtt                                                                                 >  1:4471547/1‑150 (MQ=255)
                                                                  tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                <  2:4225014/150‑1 (MQ=255)
                                                                  tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAgaatt                                                                                >  2:261875/1‑146 (MQ=255)
                                                                     ccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTcc                                                                             >  1:4038682/1‑150 (MQ=255)
                                                                      cTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTccc                                                                            <  2:2965628/150‑1 (MQ=255)
                                                                      cTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTccc                                                                            >  1:1526890/1‑150 (MQ=255)
                                                                        tCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCgg                                                                          <  2:2923329/150‑1 (MQ=255)
                                                                          aTTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGAc                                                                        >  1:1897880/1‑150 (MQ=255)
                                                                          aTTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGAc                                                                        <  2:3976816/150‑1 (MQ=255)
                                                                          aTTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGAc                                                                        <  2:930834/150‑1 (MQ=255)
                                                                            tGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAg                                                                      >  1:3968147/1‑150 (MQ=255)
                                                                            tGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAg                                                                      >  1:2628485/1‑150 (MQ=255)
                                                                             gTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGt                                                                     <  2:1907892/150‑1 (MQ=255)
                                                                               aTCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTc                                                                   >  2:645487/1‑150 (MQ=255)
                                                                                   aCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAg                                                               >  2:1027315/1‑150 (MQ=255)
                                                                                   aCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAg                                                               >  2:3359969/1‑150 (MQ=255)
                                                                                         aCATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAAt                                                         <  1:1703568/150‑1 (MQ=255)
                                                                                         aCATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAAAAGCTTTTCCCGGACAGTTCAAAGCGTAAt                                                         >  2:3439157/1‑150 (MQ=255)
                                                                                          cATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATg                                                        <  2:226245/150‑1 (MQ=255)
                                                                                             cACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTac                                                     <  2:3521332/150‑1 (MQ=255)
                                                                                                tCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTacgac                                                  <  1:99668/150‑1 (MQ=255)
                                                                                                 cAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTacgacg                                                 <  2:3791861/150‑1 (MQ=255)
                                                                                                        tGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTcc                                          >  1:177831/1‑150 (MQ=255)
                                                                                                         gAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCa                                         <  1:4198599/150‑1 (MQ=255)
                                                                                                          aaTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAg                                        >  1:3328422/1‑150 (MQ=255)
                                                                                                          aaTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAg                                        <  2:1397582/150‑1 (MQ=255)
                                                                                                          aaTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAg                                        <  2:3290002/150‑1 (MQ=255)
                                                                                                           aTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGt                                       >  2:1568918/1‑150 (MQ=255)
                                                                                                             aGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTc                                     >  1:1843276/1‑150 (MQ=255)
                                                                                                               cGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCAc                                   >  1:1656258/1‑150 (MQ=255)
                                                                                                                gTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACg                                  <  2:970435/150‑1 (MQ=255)
                                                                                                                gTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACg                                  >  2:890164/1‑150 (MQ=255)
                                                                                                                gTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACg                                  <  1:2804713/150‑1 (MQ=255)
                                                                                                                gTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACg                                  <  1:3538345/150‑1 (MQ=255)
                                                                                                                  gCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGaa                                >  2:2975354/1‑150 (MQ=255)
                                                                                                                        ctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCt                          >  2:1542430/1‑150 (MQ=255)
                                                                                                                          ctgtctgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTGTCCCGGACAGTTCTAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCg                        <  2:1776189/150‑1 (MQ=255)
                                                                                                                               tgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTACTGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGtt                   <  2:2658736/150‑1 (MQ=255)
                                                                                                                               tgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGtt                   >  1:2535997/1‑150 (MQ=255)
                                                                                                                                gcgcCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTc                  <  1:2255660/150‑1 (MQ=255)
                                                                                                                                 cgcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCa                 <  2:4443301/150‑1 (MQ=255)
                                                                                                                                  gcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAg                >  1:1338726/1‑150 (MQ=255)
                                                                                                                                   cccAAAACGGGCATCAGCTATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCACAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGt               <  2:1579361/150‑1 (MQ=255)
                                                                                                                                   cccAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGt               >  2:3939416/1‑150 (MQ=255)
                                                                                                                                    ccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGtt              >  1:4356002/1‑150 (MQ=255)
                                                                                                                                     cAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt             <  2:1861293/150‑1 (MQ=255)
                                                                                                                                     cAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt             <  1:1109776/150‑1 (MQ=255)
                                                                                                                                      aaaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt            <  2:1642582/150‑1 (MQ=255)
                                                                                                                                      aaaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt            <  2:2140584/150‑1 (MQ=255)
                                                                                                                                      aaaaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt            <  1:3621520/150‑1 (MQ=255)
                                                                                                                                       aaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttttt           <  1:837119/150‑1 (MQ=255)
                                                                                                                                       aaaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttttt           >  2:2461912/1‑150 (MQ=255)
                                                                                                                                        aaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttttt          <  2:4442055/150‑1 (MQ=255)
                                                                                                                                          cGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGa        <  2:1872589/150‑1 (MQ=255)
                                                                                                                                           gggCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGAc       >  1:2208969/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTG  >  CP009273/3798962‑3799255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCT  >  CP009273/3798960‑3799254
                                                                                                                                                    |                                                                                                                                                  
AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAG                                                                                                                                                   >  SRR19973073.3093187/1‑150 (MQ=60)
AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAG                                                                                                                                                   >  SRR19973073.3093528/1‑150 (MQ=60)
  TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCA                                                                                                                                                 <  SRR19973073.2586935/150‑1 (MQ=60)
  TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCA                                                                                                                                                 <  SRR19973073.2586932/150‑1 (MQ=60)
  TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCA                                                                                                                                                 <  SRR19973073.3833063/150‑1 (MQ=60)
  TGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCA                                                                                                                                                 >  SRR19973073.3848663/1‑150 (MQ=60)
      ATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGA                                                                                                                                             >  SRR19973073.2844645/1‑150 (MQ=60)
      ATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGA                                                                                                                                             >  SRR19973073.2844732/1‑150 (MQ=60)
       TCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAG                                                                                                                                            <  SRR19973073.777925/150‑1 (MQ=60)
        CTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGA                                                                                                                                           <  SRR19973073.1797763/150‑1 (MQ=60)
           CGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTA                                                                                                                                        <  SRR19973073.3949939/150‑1 (MQ=60)
             GTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGA                                                                                                                                      >  SRR19973073.710374/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.3898353/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.548174/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.548202/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.548281/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.2666980/1‑150 (MQ=60)
              TAATAATAAATGATGCGCGAGTTAATGGATTTAACACTTTTTCGCCAAACCCAATGCCCTTCAGTGTATCAACGCCAACATCACTCATGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGAT                                                                                                                                     >  SRR19973073.3984941/1‑150 (MQ=60)
               AATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATT                                                                                                                                    >  SRR19973073.3942668/1‑150 (MQ=60)
               AATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATT                                                                                                                                    >  SRR19973073.3113980/1‑150 (MQ=60)
               AATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATT                                                                                                                                    >  SRR19973073.3113942/1‑150 (MQ=60)
               AATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATT                                                                                                                                    <  SRR19973073.2505327/150‑1 (MQ=60)
                  AATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTT                                                                                                                                 <  SRR19973073.2757629/150‑1 (MQ=60)
                      AATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATA                                                                                                                             >  SRR19973073.1152391/1‑150 (MQ=60)
                      AATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATA                                                                                                                             >  SRR19973073.1152272/1‑150 (MQ=60)
                      AATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATA                                                                                                                             >  SRR19973073.1876426/1‑150 (MQ=60)
                       ATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAA                                                                                                                            <  SRR19973073.2087218/150‑1 (MQ=60)
                            GCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCT                                                                                                                       <  SRR19973073.4381117/150‑1 (MQ=60)
                             CGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTT                                                                                                                      <  SRR19973073.1967640/150‑1 (MQ=60)
                              GCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTT                                                                                                                     <  SRR19973073.4307748/150‑1 (MQ=60)
                              GCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTT                                                                                                                     <  SRR19973073.1790013/150‑1 (MQ=60)
                               CGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCAAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCTTCCGCAATGAGAGTAGATTTTTTATAATCTCTTTT                                                                                                                    <  SRR19973073.3851063/150‑1 (MQ=60)
                                     AATGGATTTAACCCTTTTTCGCCAAACCCACTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTC                                                                                                              <  SRR19973073.3893468/150‑1 (MQ=60)
                                     AATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTC                                                                                                              >  SRR19973073.2108578/1‑150 (MQ=60)
                                     AATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTC                                                                                                              <  SRR19973073.2993035/150‑1 (MQ=60)
                                     AATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTC                                                                                                              <  SRR19973073.3893455/150‑1 (MQ=60)
                                     AATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTC                                                                                                              <  SRR19973073.3893460/150‑1 (MQ=60)
                                      ATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCG                                                                                                             <  SRR19973073.3690612/150‑1 (MQ=60)
                                      ATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCG                                                                                                             <  SRR19973073.3784735/150‑1 (MQ=60)
                                       TGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGT                                                                                                            >  SRR19973073.3070430/1‑150 (MQ=60)
                                         GATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCC                                                                                                          >  SRR19973073.3278071/1‑150 (MQ=60)
                                         GATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCC                                                                                                          >  SRR19973073.894158/1‑150 (MQ=60)
                                         GATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCC                                                                                                          >  SRR19973073.894213/1‑150 (MQ=60)
                                          ATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCC                                                                                                         >  SRR19973073.1812055/1‑150 (MQ=60)
                                          ATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCC                                                                                                         >  SRR19973073.2741886/1‑150 (MQ=60)
                                           TTTAACCCCTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCC                                                                                                        <  SRR19973073.2128480/150‑1 (MQ=60)
                                            TTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCT                                                                                                       >  SRR19973073.4061280/1‑150 (MQ=60)
                                            TTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCT                                                                                                       <  SRR19973073.1794767/150‑1 (MQ=60)
                                            TTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCT                                                                                                       <  SRR19973073.2843744/150‑1 (MQ=60)
                                             TAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTT                                                                                                      <  SRR19973073.1843409/150‑1 (MQ=60)
                                               ACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGT                                                                                                    <  SRR19973073.1391596/150‑1 (MQ=60)
                                                  CTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCC                                                                                                 <  SRR19973073.3564629/150‑1 (MQ=60)
                                                   TTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCA                                                                                                <  SRR19973073.55466/150‑1 (MQ=60)
                                                   TTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCA                                                                                                <  SRR19973073.55464/150‑1 (MQ=60)
                                                   TTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCA                                                                                                <  SRR19973073.113348/150‑1 (MQ=60)
                                                     TTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATT                                                                                              >  SRR19973073.98732/1‑150 (MQ=60)
                                                      TTCGCCAAACCCAAGGCCCTTCATTGTATCATCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTT                                                                                             <  SRR19973073.1233219/150‑1 (MQ=60)
                                                       TCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTA                                                                                            <  SRR19973073.2910154/150‑1 (MQ=60)
                                                       TCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTA                                                                                            >  SRR19973073.3815524/1‑150 (MQ=60)
                                                       TCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTA                                                                                            >  SRR19973073.1490270/1‑150 (MQ=60)
                                                       TCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTA                                                                                            >  SRR19973073.1490278/1‑150 (MQ=60)
                                                        CGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAA                                                                                           >  SRR19973073.1145894/1‑150 (MQ=60)
                                                         GCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAG                                                                                          <  SRR19973073.4489362/150‑1 (MQ=60)
                                                          CCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGA                                                                                         <  SRR19973073.1841867/150‑1 (MQ=60)
                                                          CCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGA                                                                                         <  SRR19973073.1232754/150‑1 (MQ=60)
                                                          CCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGA                                                                                         >  SRR19973073.3422663/1‑150 (MQ=60)
                                                          CCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGA                                                                                         >  SRR19973073.2824819/1‑150 (MQ=60)
                                                          CCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGA                                                                                         >  SRR19973073.2824805/1‑150 (MQ=60)
                                                            AAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAA                                                                                       >  SRR19973073.3715131/1‑150 (MQ=60)
                                                             AACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAA                                                                                      <  SRR19973073.433754/150‑1 (MQ=60)
                                                                CCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAG                                                                                   <  SRR19973073.142418/150‑1 (MQ=60)
                                                                CCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAG                                                                                   >  SRR19973073.805103/1‑150 (MQ=60)
                                                                 CAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGC                                                                                  >  SRR19973073.2872671/1‑150 (MQ=60)
                                                                  ACTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCT                                                                                 <  SRR19973073.1373036/150‑1 (MQ=60)
                                                                  AATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCCTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCT                                                                                 >  SRR19973073.4069932/1‑150 (MQ=60)
                                                                   ATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTT                                                                                >  SRR19973073.4480539/1‑150 (MQ=60)
                                                                   ATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTT                                                                                >  SRR19973073.4480529/1‑150 (MQ=60)
                                                                    TGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTT                                                                               <  SRR19973073.1397042/150‑1 (MQ=60)
                                                                    TGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTT                                                                               <  SRR19973073.1397090/150‑1 (MQ=60)
                                                                      CCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTC                                                                             <  SRR19973073.4250194/150‑1 (MQ=60)
                                                                      CCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTC                                                                             >  SRR19973073.3620754/1‑150 (MQ=60)
                                                                       CCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCC                                                                            >  SRR19973073.4046763/1‑150 (MQ=60)
                                                                       CCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCC                                                                            >  SRR19973073.1121122/1‑150 (MQ=60)
                                                                       CCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTAC                                                                            >  SRR19973073.4046956/1‑150 (MQ=60)
                                                                        CTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCC                                                                           >  SRR19973073.1530029/1‑150 (MQ=60)
                                                                        CTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCC                                                                           >  SRR19973073.1670045/1‑150 (MQ=60)
                                                                         TTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCG                                                                          <  SRR19973073.412695/150‑1 (MQ=60)
                                                                         TTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCG                                                                          >  SRR19973073.3964394/1‑150 (MQ=60)
                                                                         TTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCG                                                                          >  SRR19973073.3964391/1‑150 (MQ=60)
                                                                            ATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGAC                                                                       >  SRR19973073.1901750/1‑150 (MQ=60)
                                                                              TGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAG                                                                     >  SRR19973073.3976056/1‑150 (MQ=60)
                                                                              TGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAG                                                                     >  SRR19973073.2633886/1‑150 (MQ=60)
                                                                               GTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGT                                                                    <  SRR19973073.1823556/150‑1 (MQ=60)
                                                                                 ATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTC                                                                  <  SRR19973073.1740740/150‑1 (MQ=60)
                                                                                    AACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAA                                                               >  SRR19973073.3575983/1‑150 (MQ=60)
                                                                                    AACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAA                                                               >  SRR19973073.2039992/1‑150 (MQ=60)
                                                                                      CGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGC                                                             <  SRR19973073.809047/150‑1 (MQ=60)
                                                                                           ACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAAT                                                        <  SRR19973073.1707102/150‑1 (MQ=60)
                                                                                           ACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAAT                                                        <  SRR19973073.892920/150‑1 (MQ=60)
                                                                                            CATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATG                                                       >  SRR19973073.277387/1‑150 (MQ=60)
                                                                                             ATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGT                                                      <  SRR19973073.3301998/150‑1 (MQ=60)
                                                                                             ATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGT                                                      <  SRR19973073.4055913/150‑1 (MQ=60)
                                                                                               CACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTAC                                                    >  SRR19973073.224803/1‑150 (MQ=60)
                                                                                                  TCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGAC                                                 <  SRR19973073.99913/150‑1 (MQ=60)
                                                                                                    AGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGA                                               <  SRR19973073.1317500/150‑1 (MQ=60)
                                                                                                        GATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCT                                           >  SRR19973073.1755226/1‑150 (MQ=60)
                                                                                                        GATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCT                                           <  SRR19973073.1874713/150‑1 (MQ=60)
                                                                                                        GATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCT                                           >  SRR19973073.923497/1‑150 (MQ=60)
                                                                                                          TGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCC                                         >  SRR19973073.178251/1‑150 (MQ=60)
                                                                                                           GAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCA                                        <  SRR19973073.4206935/150‑1 (MQ=60)
                                                                                                           GAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCA                                        <  SRR19973073.333515/150‑1 (MQ=60)
                                                                                                            AATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAG                                       >  SRR19973073.3335155/1‑150 (MQ=60)
                                                                                                            AATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAG                                       >  SRR19973073.1610315/1‑150 (MQ=60)
                                                                                                              TAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTT                                     >  SRR19973073.2811292/1‑150 (MQ=60)
                                                                                                               AGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTC                                    >  SRR19973073.1847072/1‑150 (MQ=60)
                                                                                                                 CGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCAC                                  >  SRR19973073.1659692/1‑150 (MQ=60)
                                                                                                                 CGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCAC                                  >  SRR19973073.1659554/1‑150 (MQ=60)
                                                                                                                  GTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACG                                 >  SRR19973073.3228408/1‑150 (MQ=60)
                                                                                                                  GTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACG                                 <  SRR19973073.3545542/150‑1 (MQ=60)
                                                                                                                  GTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACG                                 <  SRR19973073.3545431/150‑1 (MQ=60)
                                                                                                                  GTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACG                                 <  SRR19973073.2810463/150‑1 (MQ=60)
                                                                                                                                 TGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTT                  >  SRR19973073.2541234/1‑150 (MQ=60)
                                                                                                                                  GCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTC                 <  SRR19973073.2260291/150‑1 (MQ=60)
                                                                                                                                    GCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAG               >  SRR19973073.2474696/1‑150 (MQ=60)
                                                                                                                                    GCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAG               >  SRR19973073.1341570/1‑150 (MQ=60)
                                                                                                                                    GCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAG               >  SRR19973073.1341477/1‑150 (MQ=60)
                                                                                                                                      CCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTT             >  SRR19973073.4364723/1‑150 (MQ=60)
                                                                                                                                      CCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTT             >  SRR19973073.4364675/1‑150 (MQ=60)
                                                                                                                                       CAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTT            <  SRR19973073.1112047/150‑1 (MQ=60)
                                                                                                                                        AAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTT           <  SRR19973073.3629409/150‑1 (MQ=60)
                                                                                                                                        AAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTT           <  SRR19973073.3628793/150‑1 (MQ=60)
                                                                                                                                         AAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTT          <  SRR19973073.838840/150‑1 (MQ=60)
                                                                                                                                          AACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTT         <  SRR19973073.1188487/150‑1 (MQ=60)
                                                                                                                                             GGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGAC      >  SRR19973073.2213556/1‑150 (MQ=60)
                                                                                                                                                 ATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCT  <  SRR19973073.339228/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
AGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCT  >  CP009273/3798960‑3799254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑