Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,799,108 C→A 40.8% R68L (CGG→CTG)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,799,1080CA40.8% 313.9 / 297.8 263R68L (CGG→CTG) waaPkinase that phosphorylates core heptose of lipopolysaccharide
Reads supporting (aligned to +/- strand):  ref base C (50/105);  new base A (54/54);  total (104/159)
Fisher's exact test for biased strand distribution p-value = 4.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

GAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCA  >  CP009273/3798959‑3799257
                                                                                                                                                     |                                                                                                                                                     
gAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCACTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATcc                                                                                                                                                       <  2:3355575/150‑1 (MQ=255)
gAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATcc                                                                                                                                                       <  1:4075397/150‑1 (MQ=255)
   tGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCa                                                                                                                                                    >  1:238417/1‑150 (MQ=255)
    gagaTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCaa                                                                                                                                                   >  2:3610524/1‑150 (MQ=255)
     agaTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACACAGGCGATGAATAGCGTGCCACTCACTGTCAGCGCCCAAAACGGGCATCAGCAAt                                                                                                                                                  >  1:3170014/1‑150 (MQ=255)
       aTCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCTATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATga                                                                                                                                                <  2:2607434/150‑1 (MQ=255)
         cTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATgaga                                                                                                                                              <  2:1509137/150‑1 (MQ=255)
          ttCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGagag                                                                                                                                             <  1:667147/150‑1 (MQ=255)
           tCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGt                                                                                                                                            <  2:2073601/150‑1 (MQ=255)
             ggTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAg                                                                                                                                          <  1:3055525/150‑1 (MQ=255)
               taataataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGAt                                                                                                                                        <  1:1174526/150‑1 (MQ=255)
                aataatatATGATGCGCGAGTTTTTGGATTTAACGCTTTTGAGCAAAACCCATTGCCGTTGATTGTATCAACGCCAACATCACTGAGGCTTTGTATTGCGTTCCTCTCTCTGTCTGCGCCCAAAACGGTCATCAGCAATGAGAGTAGAtt                                                                                                                                       <  2:2731881/150‑1 (MQ=255)
                    ataaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGAtttttt                                                                                                                                   <  2:1662065/150‑1 (MQ=255)
                     taaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTta                                                                                                                                  <  1:3411435/150‑1 (MQ=255)
                     taaATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTta                                                                                                                                  <  2:492954/150‑1 (MQ=255)
                       aaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTtata                                                                                                                                <  2:1279597/150‑1 (MQ=255)
                       aaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTtata                                                                                                                                <  1:934841/150‑1 (MQ=255)
                       aaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTtata                                                                                                                                >  2:1105958/1‑150 (MQ=255)
                       aaTGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTtata                                                                                                                                >  1:56615/1‑150 (MQ=255)
                             gcgcGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATctct                                                                                                                          <  1:2484564/150‑1 (MQ=255)
                               gcgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCttt                                                                                                                        <  1:2335540/150‑1 (MQ=255)
                                cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                       <  2:1972280/150‑1 (MQ=255)
                                cgAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                       >  2:2900354/1‑150 (MQ=255)
                                cgAGATAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCtttt                                                                                                                       <  2:1972273/150‑1 (MQ=255)
                                 gAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTa                                                                                                                      >  1:3887578/1‑150 (MQ=255)
                                  aGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTaa                                                                                                                     <  2:4149081/150‑1 (MQ=255)
                                    tttaTGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATg                                                                                                                   <  2:3740673/147‑1 (MQ=255)
                                     tAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgt                                                                                                                  >  2:3059279/1‑150 (MQ=255)
                                     tAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgt                                                                                                                  >  2:2325673/1‑150 (MQ=255)
                                         ggATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATgtcgtc                                                                                                              <  2:250393/150‑1 (MQ=255)
                                              tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         <  2:2583390/150‑1 (MQ=255)
                                              tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         <  2:3713419/150‑1 (MQ=255)
                                              tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATGTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         >  1:3563046/1‑150 (MQ=255)
                                              tAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAAAGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCtt                                                                                                         >  2:35441/1‑150 (MQ=255)
                                                aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgt                                                                                                       >  1:3787783/1‑150 (MQ=255)
                                                aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTGTTTATAATCTCTTTTAATGTCTTCACCTtgt                                                                                                       >  2:1138168/1‑150 (MQ=255)
                                                aCCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgt                                                                                                       <  1:1533247/150‑1 (MQ=255)
                                                 cccTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTtgtg                                                                                                      <  1:1595100/150‑1 (MQ=255)
                                                   cTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGcc                                                                                                    >  1:769555/1‑150 (MQ=255)
                                                    tttttCTCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGATTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCa                                                                                                   <  2:2281834/150‑1 (MQ=255)
                                                      tttCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCAtt                                                                                                 <  1:1053384/150‑1 (MQ=255)
                                                        tCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTa                                                                                               <  1:4085008/150‑1 (MQ=255)
                                                          gcaaaaCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAg                                                                                             <  2:811412/147‑1 (MQ=255)
                                                          gCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAg                                                                                             <  2:2931617/150‑1 (MQ=255)
                                                               aCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGTCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAAt                                                                                        <  2:2095582/150‑1 (MQ=255)
                                                               aCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAAt                                                                                        <  1:2630999/150‑1 (MQ=255)
                                                               aCCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAAt                                                                                        <  1:228028/150‑1 (MQ=255)
                                                                cccAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATa                                                                                       <  2:2207261/150‑1 (MQ=255)
                                                                 ccAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAg                                                                                      <  2:2436854/150‑1 (MQ=255)
                                                                   aaTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCt                                                                                    >  1:2759892/1‑150 (MQ=255)
                                                                    aTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCtt                                                                                   <  2:3590914/150‑1 (MQ=255)
                                                                    aTGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCtt                                                                                   <  2:1542932/150‑1 (MQ=255)
                                                                     tGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCttt                                                                                  <  1:824741/150‑1 (MQ=255)
                                                                        ccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTcc                                                                               <  2:3184165/150‑1 (MQ=255)
                                                                        ccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTcc                                                                               <  2:746761/150‑1 (MQ=255)
                                                                        ccTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTcc                                                                               >  2:1179527/1‑150 (MQ=255)
                                                                         cTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTccc                                                                              >  2:3469614/1‑150 (MQ=255)
                                                                           tCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCgg                                                                            <  2:4073849/150‑1 (MQ=255)
                                                                           tCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCgg                                                                            <  2:3041758/150‑1 (MQ=255)
                                                                             aTTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGAc                                                                          <  2:3917963/150‑1 (MQ=255)
                                                                                gTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCGGGACAGt                                                                       >  2:958176/1‑150 (MQ=255)
                                                                                  aTCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGTGAATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTc                                                                     <  2:1838429/150‑1 (MQ=255)
                                                                                      aCGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAg                                                                 <  1:367484/150‑1 (MQ=255)
                                                                                       cGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGc                                                                <  2:1376323/150‑1 (MQ=255)
                                                                                        gCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCg                                                               >  1:1837509/1‑150 (MQ=255)
                                                                                        gCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCg                                                               >  1:488147/1‑150 (MQ=255)
                                                                                        gCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCg                                                               >  1:337243/1‑150 (MQ=255)
                                                                                           aaCATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTaa                                                            >  2:2800036/1‑150 (MQ=255)
                                                                                            aCATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAAt                                                           <  1:2749420/150‑1 (MQ=255)
                                                                                              aTCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGt                                                         >  1:504341/1‑150 (MQ=255)
                                                                                                        cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               >  1:698724/1‑150 (MQ=255)
                                                                                                        cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               >  2:1146212/1‑150 (MQ=255)
                                                                                                        cGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtc                                               <  1:3489892/150‑1 (MQ=255)
                                                                                                         gATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtct                                              <  1:1536192/150‑1 (MQ=255)
                                                                                                         gATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtct                                              <  1:3589209/150‑1 (MQ=255)
                                                                                                          aTGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGtctc                                             <  1:2504632/150‑1 (MQ=255)
                                                                                                            gAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACTACGAGTCTCCa                                           >  1:2124691/1‑150 (MQ=255)
                                                                                                               tAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGtt                                        <  1:861395/150‑1 (MQ=255)
                                                                                                                    tGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGa                                   <  2:4052358/150‑1 (MQ=255)
                                                                                                                    tGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGa                                   >  1:598967/1‑150 (MQ=255)
                                                                                                                      ccACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGaaa                                 >  1:1670581/1‑150 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  1:2550065/150‑1 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  1:467035/150‑1 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  2:3295660/150‑1 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  1:3721672/150‑1 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  2:3887578/150‑1 (MQ=255)
                                                                                                                       cACTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAg                                <  2:311567/150‑1 (MQ=255)
                                                                                                                        aCTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGa                               >  2:3963113/1‑150 (MQ=255)
                                                                                                                                tctgCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCg                       >  1:1685508/1‑150 (MQ=255)
                                                                                                                                   gcgcCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTc                    <  2:437696/150‑1 (MQ=255)
                                                                                                                                    cgcCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCa                   <  2:107310/150‑1 (MQ=255)
                                                                                                                                      cccAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGt                 <  2:3124520/150‑1 (MQ=255)
                                                                                                                                        cAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt               <  2:2437317/150‑1 (MQ=255)
                                                                                                                                        cAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt               >  1:541312/1‑150 (MQ=255)
                                                                                                                                         aaaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt              <  2:1752525/150‑1 (MQ=255)
                                                                                                                                         aaaaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt              >  1:1422117/1‑150 (MQ=255)
                                                                                                                                           aaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttttt            <  1:369282/150‑1 (MQ=255)
                                                                                                                                            aCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTg           <  1:932127/150‑1 (MQ=255)
                                                                                                                                                gCAGCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTGGTGCCATTTCAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACct       <  2:709710/150‑1 (MQ=255)
                                                                                                                                                    ccGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGc   >  2:326779/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCA  >  CP009273/3798959‑3799257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A