Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,860,327 C→A 91.1% intergenic (‑70/+42) ibpB ← / ← ibpA heat shock chaperone/heat shock chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,860,3270CA91.1% 741.0 / 66.7 260intergenic (‑70/+42)ibpB/ibpAheat shock chaperone/heat shock chaperone
Reads supporting (aligned to +/- strand):  ref base C (13/10);  new base A (124/112);  total (138/122)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTT  >  CP009273/3860182‑3860473
                                                                                                                                                 |                                                                                                                                                  
cGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGc                                                                                                                                                <  2:1302320/150‑1 (MQ=255)
cGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGc                                                                                                                                                <  2:187853/150‑1 (MQ=255)
 gTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCa                                                                                                                                               >  2:83380/1‑150 (MQ=255)
  ttttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAt                                                                                                                                              <  1:1262679/150‑1 (MQ=255)
     tGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGga                                                                                                                                           <  1:2970462/150‑1 (MQ=255)
       cAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGgaga                                                                                                                                         >  1:3991503/1‑150 (MQ=255)
        aGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGAt                                                                                                                                        >  2:3668392/1‑150 (MQ=255)
        aGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGAt                                                                                                                                        <  1:1356111/150‑1 (MQ=255)
        aGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGAt                                                                                                                                        >  1:607117/1‑150 (MQ=255)
        aGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGAt                                                                                                                                        <  2:2551012/150‑1 (MQ=255)
         gTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATg                                                                                                                                       <  2:4319530/150‑1 (MQ=255)
            cGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATTCTCCCTGACGGAGAGCATGGAGATGTCa                                                                                                                                    >  1:2514473/1‑150 (MQ=255)
             gTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAg                                                                                                                                   <  2:2996459/150‑1 (MQ=255)
                      tttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCa                                                                                                                          <  1:4535665/150‑1 (MQ=255)
                       ttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAg                                                                                                                         >  2:2572609/1‑150 (MQ=255)
                       ttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAg                                                                                                                         >  2:2781206/1‑150 (MQ=255)
                           cAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAggcgg                                                                                                                     >  2:2351136/1‑150 (MQ=255)
                            aaaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAggcggc                                                                                                                    >  1:2097471/1‑150 (MQ=255)
                            aaaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAggcggc                                                                                                                    >  1:3826548/1‑150 (MQ=255)
                             aaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   >  2:1951381/1‑150 (MQ=255)
                             aaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   >  1:3114233/1‑150 (MQ=255)
                             aaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   <  1:607400/150‑1 (MQ=255)
                               aCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCtt                                                                                                                 >  1:2927518/1‑150 (MQ=255)
                                ccGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTa                                                                                                                <  1:83380/150‑1 (MQ=255)
                                 cGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCGTAg                                                                                                               >  2:291146/1‑150 (MQ=255)
                                  gATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              <  1:3819218/150‑1 (MQ=255)
                                  gATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              >  1:4349999/1‑150 (MQ=255)
                                    tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGa                                                                                                            <  2:4222671/150‑1 (MQ=255)
                                      cATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                          <  1:1114424/150‑1 (MQ=255)
                                      cATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGATATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                          >  2:231252/1‑150 (MQ=255)
                                      cATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                          <  2:3823092/150‑1 (MQ=255)
                                       aTTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAtt                                                                                                         >  1:3648242/1‑150 (MQ=255)
                                            cGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTg                                                                                                    >  1:3010236/1‑150 (MQ=255)
                                              cATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAt                                                                                                  <  2:1452707/150‑1 (MQ=255)
                                               aTCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAtt                                                                                                 >  1:961440/1‑150 (MQ=255)
                                                 cAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTc                                                                                               >  2:196506/1‑150 (MQ=255)
                                                 cAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTc                                                                                               <  2:1470739/150‑1 (MQ=255)
                                                    tGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAt                                                                                            <  1:2151228/150‑1 (MQ=255)
                                                    tGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAt                                                                                            <  2:1421564/150‑1 (MQ=255)
                                                    tGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAt                                                                                            <  1:4532750/150‑1 (MQ=255)
                                                    tGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAt                                                                                            <  1:1283705/150‑1 (MQ=255)
                                                     ggggATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATa                                                                                           <  1:3274058/150‑1 (MQ=255)
                                                         aTAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgc                                                                                       >  1:3307070/1‑150 (MQ=255)
                                                          tAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgcg                                                                                      >  1:2060795/1‑150 (MQ=255)
                                                          tAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgcg                                                                                      <  1:577229/150‑1 (MQ=255)
                                                           aaaTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgcgc                                                                                     >  1:4507256/1‑150 (MQ=255)
                                                            aaTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGcgcg                                                                                    >  2:3851623/1‑150 (MQ=255)
                                                                gAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGttt                                                                                <  2:1674346/150‑1 (MQ=255)
                                                                gAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGttt                                                                                <  2:4548101/150‑1 (MQ=255)
                                                                 aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                               >  1:1251667/1‑150 (MQ=255)
                                                                 aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                               <  1:3544030/150‑1 (MQ=255)
                                                                   gTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttttt                                                                             >  2:677222/1‑150 (MQ=255)
                                                                    ttACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTc                                                                            <  2:1772610/150‑1 (MQ=255)
                                                                       cGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCt                                                                         <  1:1797456/150‑1 (MQ=255)
                                                                        gCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCtt                                                                        >  2:2020545/1‑150 (MQ=255)
                                                                          aTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTcc                                                                      <  2:3236889/150‑1 (MQ=255)
                                                                          aTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTcc                                                                      >  1:1738201/1‑150 (MQ=255)
                                                                          aTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTcc                                                                      >  2:3168332/1‑150 (MQ=255)
                                                                          aTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCGCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTcc                                                                      <  1:419394/150‑1 (MQ=255)
                                                                           tAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCg                                                                     >  1:1893064/1‑150 (MQ=255)
                                                                           gaGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCCGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCg                                                                     <  1:3499206/149‑1 (MQ=255)
                                                                               cATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAAt                                                                 >  1:3478326/1‑150 (MQ=255)
                                                                                aTTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATc                                                                <  2:2408374/150‑1 (MQ=255)
                                                                                 tttCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCa                                                               <  1:2801176/150‑1 (MQ=255)
                                                                                 tttCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCa                                                               >  1:920791/1‑150 (MQ=255)
                                                                                    ctcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgc                                                            >  1:4457275/1‑150 (MQ=255)
                                                                                     tcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgcg                                                           >  2:3314063/1‑150 (MQ=255)
                                                                                      ccTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgcgg                                                          >  2:1200079/1‑149 (MQ=255)
                                                                                      ccTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGt                                                          <  1:2026436/150‑1 (MQ=255)
                                                                                       cTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGtt                                                         >  2:2202053/1‑150 (MQ=255)
                                                                                          cTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCga                                                      >  2:3544835/1‑150 (MQ=255)
                                                                                          cTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCga                                                      >  1:3593307/1‑150 (MQ=255)
                                                                                            aagaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    <  2:4049390/150‑1 (MQ=255)
                                                                                            aagaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    <  2:368189/150‑1 (MQ=255)
                                                                                               aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCg                                                 >  1:3780878/1‑150 (MQ=255)
                                                                                                agCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGa                                                >  2:1563951/1‑150 (MQ=255)
                                                                                                agCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGa                                                >  2:4000457/1‑150 (MQ=255)
                                                                                                 gCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGat                                               >  1:1835366/1‑150 (MQ=255)
                                                                                                  cGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGGTCGAGATCGata                                              >  2:1832274/1‑150 (MQ=255)
                                                                                                           aCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCaaa                                     >  2:2437139/1‑150 (MQ=255)
                                                                                                             cTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAcc                                   <  1:4155166/150‑1 (MQ=255)
                                                                                                             cTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAcc                                   >  1:4294973/1‑150 (MQ=255)
                                                                                                                cAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAtt                                <  2:2082762/150‑1 (MQ=255)
                                                                                                                 taaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAttt                               >  2:1632253/2‑150 (MQ=255)
                                                                                                                 aaaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAttt                               <  1:1673120/150‑1 (MQ=255)
                                                                                                                  aaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTa                              <  1:2279742/150‑1 (MQ=255)
                                                                                                                       gAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAgg                         <  2:2670341/150‑1 (MQ=255)
                                                                                                                        aaGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGt                        >  1:4062283/1‑150 (MQ=255)
                                                                                                                         aGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGtt                       >  1:2530805/1‑150 (MQ=255)
                                                                                                                          gATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTa                      <  2:737387/150‑1 (MQ=255)
                                                                                                                           aTTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAg                     <  2:2159165/150‑1 (MQ=255)
                                                                                                                           aTTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAg                     >  2:780308/1‑150 (MQ=255)
                                                                                                                                 atatGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcaccac               <  2:156565/150‑1 (MQ=255)
                                                                                                                                    tGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGaa            <  1:3719834/150‑1 (MQ=255)
                                                                                                                                       tCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACAt         >  2:3357833/1‑150 (MQ=255)
                                                                                                                                        cccTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTACGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATg        >  2:2096093/1‑150 (MQ=255)
                                                                                                                                           tGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAAt     <  2:2951074/150‑1 (MQ=255)
                                                                                                                                            gACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg    >  1:2840271/1‑150 (MQ=255)
                                                                                                                                             aCGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGt   <  2:3739884/150‑1 (MQ=255)
                                                                                                                                              cGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGtt  <  2:4486073/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTT  >  CP009273/3860182‑3860473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  CP009273/3860179‑3860474
                                                                                                                                                    |                                                                                                                                                   
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAG                                                                                                                                                    >  SRR19973072.32058/1‑150 (MQ=60)
    GTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCA                                                                                                                                                <  SRR19973072.1744455/150‑1 (MQ=60)
     TTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAT                                                                                                                                               <  SRR19973072.1265293/150‑1 (MQ=60)
     TTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAT                                                                                                                                               <  SRR19973072.1265397/150‑1 (MQ=60)
        TGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGA                                                                                                                                            <  SRR19973072.2976788/150‑1 (MQ=60)
        TGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGA                                                                                                                                            <  SRR19973072.2976799/150‑1 (MQ=60)
          CAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGA                                                                                                                                          >  SRR19973072.3999857/1‑150 (MQ=60)
          CAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGA                                                                                                                                          >  SRR19973072.3999858/1‑150 (MQ=60)
          CAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGA                                                                                                                                          >  SRR19973072.4495541/1‑150 (MQ=60)
           AGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGAT                                                                                                                                         <  SRR19973072.1359080/150‑1 (MQ=60)
           AGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGAT                                                                                                                                         >  SRR19973072.608522/1‑150 (MQ=60)
           AGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGAT                                                                                                                                         >  SRR19973072.608514/1‑150 (MQ=60)
               CGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATTCTCCCTGACGGAGAGCATGGAGATGTCA                                                                                                                                     >  SRR19973072.2519936/1‑150 (MQ=60)
                GTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAG                                                                                                                                    >  SRR19973072.3074893/1‑150 (MQ=60)
                  TGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGC                                                                                                                                  >  SRR19973072.1894858/1‑150 (MQ=60)
                    GCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCG                                                                                                                                <  SRR19973072.1464562/150‑1 (MQ=60)
                    GCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCG                                                                                                                                <  SRR19973072.1464492/150‑1 (MQ=60)
                         TTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCA                                                                                                                           <  SRR19973072.4545233/150‑1 (MQ=60)
                         TTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCA                                                                                                                           <  SRR19973072.4545103/150‑1 (MQ=60)
                         TTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCA                                                                                                                           >  SRR19973072.2084119/1‑150 (MQ=60)
                         TTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCA                                                                                                                           <  SRR19973072.3085289/150‑1 (MQ=60)
                           TGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGG                                                                                                                         <  SRR19973072.132057/150‑1 (MQ=60)
                               AAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGC                                                                                                                     >  SRR19973072.440052/1‑150 (MQ=60)
                               AAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGC                                                                                                                     >  SRR19973072.2102047/1‑150 (MQ=60)
                               AAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGC                                                                                                                     >  SRR19973072.3834474/1‑150 (MQ=60)
                               AAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGC                                                                                                                     >  SRR19973072.3834547/1‑150 (MQ=60)
                                AAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCC                                                                                                                    <  SRR19973072.608798/150‑1 (MQ=60)
                                AAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCC                                                                                                                    >  SRR19973072.3120800/1‑150 (MQ=60)
                                AAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAGGGAGATGTCAGGCCGCGCCAGGCGGCC                                                                                                                    >  SRR19973072.4222861/1‑150 (MQ=60)
                                  ACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTT                                                                                                                  <  SRR19973072.3010448/150‑1 (MQ=60)
                                  ACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTT                                                                                                                  >  SRR19973072.1276451/1‑150 (MQ=60)
                                  ACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTT                                                                                                                  >  SRR19973072.2933783/1‑150 (MQ=60)
                                   CCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTA                                                                                                                 <  SRR19973072.83589/150‑1 (MQ=60)
                                     GATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGG                                                                                                               >  SRR19973072.4359052/1‑150 (MQ=60)
                                     GATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGG                                                                                                               <  SRR19973072.3827206/150‑1 (MQ=60)
                                     GATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGG                                                                                                               >  SRR19973072.4359116/1‑150 (MQ=60)
                                        CCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAA                                                                                                            >  SRR19973072.2798121/1‑150 (MQ=60)
                                         CATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAT                                                                                                           <  SRR19973072.1116776/150‑1 (MQ=60)
                                          ATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATT                                                                                                          >  SRR19973072.3655933/1‑150 (MQ=60)
                                          ATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATT                                                                                                          >  SRR19973072.3655954/1‑150 (MQ=60)
                                           TTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTA                                                                                                         >  SRR19973072.3748957/1‑150 (MQ=60)
                                           TTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTA                                                                                                         >  SRR19973072.3500315/1‑150 (MQ=60)
                                            TGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAG                                                                                                        >  SRR19973072.3711380/1‑150 (MQ=60)
                                               CGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTG                                                                                                     <  SRR19973072.3309317/150‑1 (MQ=60)
                                               CGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTG                                                                                                     >  SRR19973072.3016634/1‑150 (MQ=60)
                                                  ATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATT                                                                                                  <  SRR19973072.2003773/150‑1 (MQ=60)
                                                  ATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATT                                                                                                  >  SRR19973072.963507/1‑150 (MQ=60)
                                                  ATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATT                                                                                                  >  SRR19973072.963546/1‑150 (MQ=60)
                                                     AGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCTGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCG                                                                                               >  SRR19973072.3808948/1‑150 (MQ=60)
                                                     AGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACGTAGTTGATTTCG                                                                                               >  SRR19973072.1220714/1‑150 (MQ=60)
                                                       TGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAT                                                                                             <  SRR19973072.1286478/150‑1 (MQ=60)
                                                       TGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAT                                                                                             <  SRR19973072.4542314/150‑1 (MQ=60)
                                                       TGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAT                                                                                             <  SRR19973072.4542313/150‑1 (MQ=60)
                                                       TGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAT                                                                                             <  SRR19973072.2155909/150‑1 (MQ=60)
                                                        GGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATA                                                                                            <  SRR19973072.3281008/150‑1 (MQ=60)
                                                            ATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGC                                                                                        >  SRR19973072.3314096/1‑150 (MQ=60)
                                                             TAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCG                                                                                       <  SRR19973072.578575/150‑1 (MQ=60)
                                                             TAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCG                                                                                       <  SRR19973072.578567/150‑1 (MQ=60)
                                                             TAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCG                                                                                       >  SRR19973072.2065281/1‑150 (MQ=60)
                                                              AAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGC                                                                                      >  SRR19973072.4516773/1‑150 (MQ=60)
                                                               AATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCG                                                                                     >  SRR19973072.2065189/1‑150 (MQ=60)
                                                                  CGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTT                                                                                  <  SRR19973072.1996389/150‑1 (MQ=60)
                                                                    AAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTT                                                                                >  SRR19973072.1254354/1‑150 (MQ=60)
                                                                    AAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTT                                                                                >  SRR19973072.1254395/1‑150 (MQ=60)
                                                                    AAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTT                                                                                <  SRR19973072.3551481/150‑1 (MQ=60)
                                                                       TTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCGGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTC                                                                             <  SRR19973072.4529357/150‑1 (MQ=60)
                                                                        TACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCG                                                                            >  SRR19973072.3842340/1‑150 (MQ=60)
                                                                          CGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCT                                                                          <  SRR19973072.1801391/150‑1 (MQ=60)
                                                                           GCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTT                                                                         <  SRR19973072.352184/150‑1 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       <  SRR19973072.420213/150‑1 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       >  SRR19973072.1742005/1‑150 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       >  SRR19973072.1742161/1‑150 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCGCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       <  SRR19973072.420340/150‑1 (MQ=60)
                                                                              TAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCG                                                                      >  SRR19973072.605462/1‑150 (MQ=60)
                                                                              TAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCG                                                                      >  SRR19973072.1897114/1‑150 (MQ=60)
                                                                              TAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCG                                                                      <  SRR19973072.4100046/150‑1 (MQ=60)
                                                                              GAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCCGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCG                                                                      <  SRR19973072.3506538/150‑1 (MQ=60)
                                                                                 TCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAA                                                                   >  SRR19973072.1311720/1‑150 (MQ=60)
                                                                                  CATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAAT                                                                  >  SRR19973072.3485591/1‑150 (MQ=60)
                                                                                   ATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATC                                                                 <  SRR19973072.694531/150‑1 (MQ=60)
                                                                                   ATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATC                                                                 <  SRR19973072.693837/150‑1 (MQ=60)
                                                                                   ATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATC                                                                 >  SRR19973072.3285250/1‑150 (MQ=60)
                                                                                    TTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCA                                                                >  SRR19973072.922798/1‑150 (MQ=60)
                                                                                    TTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCA                                                                >  SRR19973072.922791/1‑150 (MQ=60)
                                                                                    TTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCA                                                                <  SRR19973072.2807176/150‑1 (MQ=60)
                                                                                      TCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACG                                                              >  SRR19973072.4466077/1‑150 (MQ=60)
                                                                                       CTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGC                                                             >  SRR19973072.4466678/1‑150 (MQ=60)
                                                                                        TCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCG                                                            >  SRR19973072.4585862/1‑150 (MQ=60)
                                                                                        TCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCG                                                            <  SRR19973072.4191966/150‑1 (MQ=60)
                                                                                         CCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGT                                                           <  SRR19973072.2030822/150‑1 (MQ=60)
                                                                                           TTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTC                                                         <  SRR19973072.22986/150‑1 (MQ=60)
                                                                                            TCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCG                                                        >  SRR19973072.4312278/1‑150 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       >  SRR19973072.3600870/1‑150 (MQ=60)
                                                                                              TAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAG                                                      <  SRR19973072.3568585/150‑1 (MQ=60)
                                                                                               AAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGA                                                     >  SRR19973072.2947921/1‑150 (MQ=60)
                                                                                               AAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGA                                                     >  SRR19973072.2929546/1‑150 (MQ=60)
                                                                                                 GAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATC                                                   <  SRR19973072.3977786/150‑1 (MQ=60)
                                                                                                  AAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCG                                                  >  SRR19973072.3788809/1‑150 (MQ=60)
                                                                                                  AAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCG                                                  >  SRR19973072.914690/1‑150 (MQ=60)
                                                                                                    GCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAT                                                >  SRR19973072.1839354/1‑150 (MQ=60)
                                                                                                    GCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAT                                                <  SRR19973072.3363029/150‑1 (MQ=60)
                                                                                                    GCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAT                                                >  SRR19973072.1839217/1‑150 (MQ=60)
                                                                                                      GAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCTCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCAGGAATCACGCGTTCGAGATCGATAT                                              <  SRR19973072.2933546/150‑1 (MQ=60)
                                                                                                                CTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACC                                    >  SRR19973072.4303927/1‑150 (MQ=60)
                                                                                                                CTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACC                                    <  SRR19973072.4163877/150‑1 (MQ=60)
                                                                                                                   CAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATT                                 >  SRR19973072.2212010/1‑150 (MQ=60)
                                                                                                                   CAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACAATT                                 <  SRR19973072.3178872/150‑1 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                <  SRR19973072.1676784/150‑1 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                <  SRR19973072.1676792/150‑1 (MQ=60)
                                                                                                                     AATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTA                               <  SRR19973072.2284633/150‑1 (MQ=60)
                                                                                                                     AATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTA                               <  SRR19973072.2284616/150‑1 (MQ=60)
                                                                                                                     AATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTA                               <  SRR19973072.2284897/150‑1 (MQ=60)
                                                                                                                         CGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAG                           >  SRR19973072.2531339/1‑150 (MQ=60)
                                                                                                                           AAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGT                         >  SRR19973072.4070804/1‑150 (MQ=60)
                                                                                                                           AAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGT                         >  SRR19973072.2074045/1‑150 (MQ=60)
                                                                                                                           AAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGT                         >  SRR19973072.4070785/1‑150 (MQ=60)
                                                                                                                            AGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTT                        >  SRR19973072.3813892/1‑150 (MQ=60)
                                                                                                                            AGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTT                        >  SRR19973072.2536302/1‑150 (MQ=60)
                                                                                                                            AGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTT                        <  SRR19973072.3316644/150‑1 (MQ=60)
                                                                                                                                 CGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCAC                   <  SRR19973072.1896623/150‑1 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973072.3623724/1‑150 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973072.3623727/1‑150 (MQ=60)
                                                                                                                                     TATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACG               >  SRR19973072.21222/1‑150 (MQ=60)
                                                                                                                                       TGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAA             <  SRR19973072.3727665/150‑1 (MQ=60)
                                                                                                                                       TGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAA             >  SRR19973072.3527818/1‑150 (MQ=60)
                                                                                                                                           CCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATG         >  SRR19973072.592296/1‑150 (MQ=60)
                                                                                                                                           CCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATG         >  SRR19973072.2810237/1‑150 (MQ=60)
                                                                                                                                              TGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAAT      <  SRR19973072.2693981/150‑1 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     >  SRR19973072.2846349/1‑150 (MQ=60)
                                                                                                                                                  GGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  SRR19973072.867694/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  CP009273/3860179‑3860474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑