Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,860,327 C→A 100% intergenic (‑70/+42) ibpB ← / ← ibpA heat shock chaperone/heat shock chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,860,3270CA97.9% 612.0 / 3.3 191intergenic (‑70/+42)ibpB/ibpAheat shock chaperone/heat shock chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (112/75);  total (112/79)
Fisher's exact test for biased strand distribution p-value = 2.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

CGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTT  >  CP009273/3860182‑3860473
                                                                                                                                                 |                                                                                                                                                  
cGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGgaga                                                                                                                                                  >  2:1866455/1‑148 (MQ=255)
 gTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCGTTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGagag                                                                                                                                                 <  1:874342/148‑1 (MQ=255)
  ttttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGc                                                                                                                                                <  1:296050/148‑1 (MQ=255)
   tttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCa                                                                                                                                               <  1:661690/148‑1 (MQ=255)
   tttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCa                                                                                                                                               >  2:1561231/1‑148 (MQ=255)
            cGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGt                                                                                                                                      <  2:3728372/148‑1 (MQ=255)
              ttGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCa                                                                                                                                    >  1:2562861/1‑148 (MQ=255)
                   cAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCcg                                                                                                                               >  1:1180756/1‑148 (MQ=255)
                   cAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCcg                                                                                                                               >  2:3437745/1‑148 (MQ=255)
                      tttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCgcgc                                                                                                                            >  1:491019/1‑148 (MQ=255)
                      tttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCgcgc                                                                                                                            >  2:1139774/1‑148 (MQ=255)
                      tttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCgcgc                                                                                                                            >  2:745678/1‑148 (MQ=255)
                      tttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCgcgc                                                                                                                            >  2:938504/1‑148 (MQ=255)
                       ttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGcc                                                                                                                           <  1:972395/148‑1 (MQ=255)
                        tGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCa                                                                                                                          >  2:2468646/1‑148 (MQ=255)
                            aaaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAggcg                                                                                                                      >  2:3524015/1‑148 (MQ=255)
                               aCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   >  2:3454497/1‑148 (MQ=255)
                               aCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   <  1:1073619/148‑1 (MQ=255)
                               aCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAGGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                   >  2:2070085/1‑148 (MQ=255)
                                    tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTCAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              >  2:2505475/1‑148 (MQ=255)
                                    tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              >  2:2505487/1‑148 (MQ=255)
                                    tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              >  2:150940/1‑148 (MQ=255)
                                    tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAgg                                                                                                              >  2:1423171/1‑148 (MQ=255)
                                     ccATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAggg                                                                                                             >  2:27438/1‑148 (MQ=255)
                                     ccATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAggg                                                                                                             >  1:3748034/1‑148 (MQ=255)
                                      cATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGa                                                                                                            <  2:1967405/148‑1 (MQ=255)
                                        ttGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                          <  1:3468744/148‑1 (MQ=255)
                                        ttGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                          <  2:3683804/148‑1 (MQ=255)
                                           aCGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAg                                                                                                       <  1:2670028/148‑1 (MQ=255)
                                                tCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAt                                                                                                  <  2:2914203/148‑1 (MQ=255)
                                                 cAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAtt                                                                                                 >  2:1244332/1‑148 (MQ=255)
                                                         aTAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACg                                                                                         >  1:3542823/1‑148 (MQ=255)
                                                           aaaTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgc                                                                                       >  1:961390/1‑148 (MQ=255)
                                                            aaTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgcg                                                                                      >  2:2445563/1‑148 (MQ=255)
                                                             aTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGgcgc                                                                                     >  1:1995443/1‑148 (MQ=255)
                                                               cGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCgg                                                                                   <  1:2181778/148‑1 (MQ=255)
                                                                 aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtt                                                                                 >  1:1085255/1‑148 (MQ=255)
                                                                   gTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                               <  2:1017392/148‑1 (MQ=255)
                                                                   gTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                               <  1:3252651/148‑1 (MQ=255)
                                                                     tACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttttt                                                                             >  1:2895819/1‑148 (MQ=255)
                                                                           tAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTc                                                                       >  2:3391243/1‑148 (MQ=255)
                                                                           tAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTc                                                                       >  1:1505405/1‑148 (MQ=255)
                                                                               cATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGa                                                                   >  1:3600547/1‑148 (MQ=255)
                                                                               cATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGa                                                                   <  2:1117502/148‑1 (MQ=255)
                                                                               cATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGa                                                                   <  2:962080/148‑1 (MQ=255)
                                                                                     tcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcg                                                             <  2:3408729/148‑1 (MQ=255)
                                                                                       cTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgcg                                                           >  1:687933/1‑148 (MQ=255)
                                                                                        ttCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGt                                                          <  1:3371717/148‑1 (MQ=255)
                                                                                         tCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGtt                                                         >  1:522159/1‑148 (MQ=255)
                                                                                          cTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTc                                                        <  1:2475911/148‑1 (MQ=255)
                                                                                           tAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCg                                                       <  2:3347062/148‑1 (MQ=255)
                                                                                           tAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCg                                                       <  2:1453706/148‑1 (MQ=255)
                                                                                            aagaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCga                                                      >  1:213242/1‑148 (MQ=255)
                                                                                            aagaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCga                                                      >  2:3820324/1‑148 (MQ=255)
                                                                                            aagaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCga                                                      >  1:2142077/1‑148 (MQ=255)
                                                                                             agaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgag                                                     >  2:3512615/1‑148 (MQ=255)
                                                                                              gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    >  1:1901417/1‑148 (MQ=255)
                                                                                              gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    <  2:429810/148‑1 (MQ=255)
                                                                                              gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    <  1:6316/148‑1 (MQ=255)
                                                                                              gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCGTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgaga                                                    >  2:1376771/1‑148 (MQ=255)
                                                                                               aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTGGAGAt                                                   >  1:173541/1‑148 (MQ=255)
                                                                                               aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAt                                                   >  2:3571205/1‑148 (MQ=255)
                                                                                               aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAt                                                   <  2:491019/148‑1 (MQ=255)
                                                                                               aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAt                                                   >  1:55669/1‑148 (MQ=255)
                                                                                                 gCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCg                                                 >  1:3089811/1‑148 (MQ=255)
                                                                                                 gCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCg                                                 <  2:2668390/148‑1 (MQ=255)
                                                                                                 gCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCAGCGCCAGGCGGCCTTAGGGAAGTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCg                                                 >  2:1824720/1‑148 (MQ=255)
                                                                                                   gAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGat                                               <  1:2099745/148‑1 (MQ=255)
                                                                                                      taagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                            <  1:1372896/148‑1 (MQ=255)
                                                                                                      taagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                            >  1:3188329/1‑148 (MQ=255)
                                                                                                      taagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                            >  1:471086/1‑148 (MQ=255)
                                                                                                          taCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGc                                        >  2:42270/1‑148 (MQ=255)
                                                                                                              tGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAc                                    <  1:3124738/148‑1 (MQ=255)
                                                                                                              tGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAc                                    >  1:1624609/1‑148 (MQ=255)
                                                                                                                 aaaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAt                                 <  1:3539561/148‑1 (MQ=255)
                                                                                                                  aaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAtt                                <  2:301768/148‑1 (MQ=255)
                                                                                                                   aTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAttt                               <  2:2350018/148‑1 (MQ=255)
                                                                                                                   aTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAttt                               >  1:144972/1‑148 (MQ=255)
                                                                                                                    tCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTa                              >  1:1225460/1‑148 (MQ=255)
                                                                                                                     ccGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTAc                             >  2:3092568/1‑148 (MQ=255)
                                                                                                                        aaGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAg                          >  2:2062109/1‑148 (MQ=255)
                                                                                                                        aaGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAg                          >  2:730702/1‑148 (MQ=255)
                                                                                                                        aaGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAg                          >  2:1741836/1‑148 (MQ=255)
                                                                                                                         aGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAgg                         >  2:2346425/1‑148 (MQ=255)
                                                                                                                         aGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAgg                         >  2:55048/1‑148 (MQ=255)
                                                                                                                          gATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGt                        >  2:2758337/1‑148 (MQ=255)
                                                                                                                           aTTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGtt                       >  1:2384133/1‑148 (MQ=255)
                                                                                                                             tCGCATATGCTCCCTGACGGAGAGCAGGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAg                     >  2:3458024/1‑148 (MQ=255)
                                                                                                                                   atGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATAACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcaccac               >  1:3121654/1‑148 (MQ=255)
                                                                                                                                    tGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACg              >  1:815217/1‑148 (MQ=255)
                                                                                                                                     gCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCGTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGa             <  1:2096594/148‑1 (MQ=255)
                                                                                                                                      cTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGaa            >  1:3326799/1‑148 (MQ=255)
                                                                                                                                       tCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAAc           <  1:2300845/148‑1 (MQ=255)
                                                                                                                                            gACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGaa      <  1:745678/148‑1 (MQ=255)
                                                                                                                                             aCGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAAt     >  1:1276024/1‑148 (MQ=255)
                                                                                                                                              cGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg    >  1:1128908/1‑148 (MQ=255)
                                                                                                                                              cGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg    >  1:1128418/1‑148 (MQ=255)
                                                                                                                                              cGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg    >  1:1123588/1‑148 (MQ=255)
                                                                                                                                              cGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg    <  1:422082/148‑1 (MQ=255)
                                                                                                                                                gCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGACTGtt  <  2:836612/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTT  >  CP009273/3860182‑3860473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  CP009273/3860179‑3860474
                                                                                                                                                    |                                                                                                                                                   
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAG                                                                                                                                                    <  SRR19973069.1549670/150‑1 (MQ=60)
  GCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAG                                                                                                                                                  <  SRR19973069.2274639/150‑1 (MQ=60)
  GCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAG                                                                                                                                                  <  SRR19973069.2465907/150‑1 (MQ=60)
    GTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCGTTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCA                                                                                                                                                <  SRR19973069.880420/150‑1 (MQ=60)
     TTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAT                                                                                                                                               <  SRR19973069.298072/150‑1 (MQ=60)
      TTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATG                                                                                                                                              <  SRR19973069.666165/150‑1 (MQ=60)
         GCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAG                                                                                                                                           <  SRR19973069.3145847/150‑1 (MQ=60)
           AGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGAT                                                                                                                                         <  SRR19973069.3036645/150‑1 (MQ=60)
               CGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCA                                                                                                                                     >  SRR19973069.2579783/1‑150 (MQ=60)
                  TGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGC                                                                                                                                  >  SRR19973069.990513/1‑150 (MQ=60)
                   GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCC                                                                                                                                 <  SRR19973069.3299004/150‑1 (MQ=60)
                    GCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCG                                                                                                                                >  SRR19973069.1188913/1‑150 (MQ=60)
                       AGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGC                                                                                                                             >  SRR19973069.494557/1‑150 (MQ=60)
                          TTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAG                                                                                                                          <  SRR19973069.978905/150‑1 (MQ=60)
                               AAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGC                                                                                                                     >  SRR19973069.3613103/1‑150 (MQ=60)
                                 AACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCT                                                                                                                   <  SRR19973069.3116269/150‑1 (MQ=60)
                                  ACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTT                                                                                                                  <  SRR19973069.1081107/150‑1 (MQ=60)
                                    CGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAG                                                                                                                <  SRR19973069.2522244/150‑1 (MQ=60)
                                      ATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGG                                                                                                              >  SRR19973069.3772442/1‑150 (MQ=60)
                                           TTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTA                                                                                                         <  SRR19973069.3491111/150‑1 (MQ=60)
                                           TTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTA                                                                                                         <  SRR19973069.3491181/150‑1 (MQ=60)
                                           TTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTA                                                                                                         <  SRR19973069.3491224/150‑1 (MQ=60)
                                             GACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGT                                                                                                       <  SRR19973069.1433117/150‑1 (MQ=60)
                                              ACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTT                                                                                                      <  SRR19973069.2687932/150‑1 (MQ=60)
                                               CGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTG                                                                                                     >  SRR19973069.1998329/1‑150 (MQ=60)
                                                    CAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTC                                                                                                >  SRR19973069.1820811/1‑150 (MQ=60)
                                                       TGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGAT                                                                                             >  SRR19973069.2653896/1‑150 (MQ=60)
                                                         GGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATAC                                                                                           <  SRR19973069.3745878/150‑1 (MQ=60)
                                                          GGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACG                                                                                          >  SRR19973069.3565821/1‑150 (MQ=60)
                                                          GGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGGCAGGCCGCGCCAGGCGGCCTTCGGGACTTAGGTGATTTCGATACG                                                                                          >  SRR19973069.1658932/1‑150 (MQ=60)
                                                            ATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGC                                                                                        >  SRR19973069.967814/1‑150 (MQ=60)
                                                            ATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGCCGGCCGTAGGGAATTAGTTGATTTCGATACCGC                                                                                        >  SRR19973069.968506/1‑150 (MQ=60)
                                                              AAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGC                                                                                      >  SRR19973069.3246355/1‑150 (MQ=60)
                                                              AAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGC                                                                                      >  SRR19973069.3390949/1‑150 (MQ=60)
                                                              AAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGC                                                                                      >  SRR19973069.2009342/1‑150 (MQ=60)
                                                                  CGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTT                                                                                  >  SRR19973069.1092832/1‑150 (MQ=60)
                                                                  CGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTT                                                                                  <  SRR19973069.2196650/150‑1 (MQ=60)
                                                                     AGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTT                                                                               >  SRR19973069.1113842/1‑150 (MQ=60)
                                                                      GTTACGCATAGTCATTTCTCCTTCTAATAAGCGAGTAAGTACATGCAAATCCGAAGATTCGCATATGCTCCCTGACGGATAGCATGGAGATGTCAGGCCGCGCCAGGAGGCCTTAGGGAATTAGTTGATTTCGATACCGCGCGGTTTTTT                                                                              >  SRR19973069.1263132/1‑150 (MQ=60)
                                                                      GTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTT                                                                              <  SRR19973069.3273950/150‑1 (MQ=60)
                                                                      GTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTT                                                                              >  SRR19973069.2914922/1‑150 (MQ=60)
                                                                            CATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTC                                                                        >  SRR19973069.1515834/1‑150 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       >  SRR19973069.121718/1‑150 (MQ=60)
                                                                             ATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCC                                                                       >  SRR19973069.1279722/1‑150 (MQ=60)
                                                                                GTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGA                                                                    >  SRR19973069.3624047/1‑150 (MQ=60)
                                                                                GTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGA                                                                    >  SRR19973069.93686/1‑150 (MQ=60)
                                                                                GTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCGCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGA                                                                    <  SRR19973069.840170/150‑1 (MQ=60)
                                                                                        TCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCG                                                            >  SRR19973069.692739/1‑150 (MQ=60)
                                                                                        TCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGAGGTCCGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTGCCGGAATCACGCG                                                            >  SRR19973069.692674/1‑150 (MQ=60)
                                                                                          CTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTT                                                          >  SRR19973069.525861/1‑150 (MQ=60)
                                                                                          CTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTT                                                          >  SRR19973069.525563/1‑150 (MQ=60)
                                                                                           TTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTC                                                         <  SRR19973069.3393760/150‑1 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       >  SRR19973069.2156783/1‑150 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       >  SRR19973069.2156699/1‑150 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       >  SRR19973069.214919/1‑150 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       <  SRR19973069.2485215/150‑1 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       <  SRR19973069.2492505/150‑1 (MQ=60)
                                                                                             CTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGA                                                       >  SRR19973069.214759/1‑150 (MQ=60)
                                                                                               AAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGA                                                     >  SRR19973069.1914563/1‑150 (MQ=60)
                                                                                                AGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTGGAGAT                                                    >  SRR19973069.175009/1‑150 (MQ=60)
                                                                                                AGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAT                                                    >  SRR19973069.2173793/1‑150 (MQ=60)
                                                                                                AGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAT                                                    >  SRR19973069.56351/1‑150 (MQ=60)
                                                                                                 GAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATC                                                   <  SRR19973069.6423/150‑1 (MQ=60)
                                                                                                 GAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATC                                                   <  SRR19973069.6090/150‑1 (MQ=60)
                                                                                                  AAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCG                                                  >  SRR19973069.3110183/1‑150 (MQ=60)
                                                                                                    GCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAT                                                <  SRR19973069.2916775/150‑1 (MQ=60)
                                                                                                      GAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATAT                                              <  SRR19973069.2114246/150‑1 (MQ=60)
                                                                                                      GAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATAT                                              <  SRR19973069.2114231/150‑1 (MQ=60)
                                                                                                       AGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATA                                             >  SRR19973069.3209037/1‑150 (MQ=60)
                                                                                                       AGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATA                                             >  SRR19973069.474518/1‑150 (MQ=60)
                                                                                                         TAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACA                                           <  SRR19973069.1382458/150‑1 (MQ=60)
                                                                                                         TAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACA                                           <  SRR19973069.1381233/150‑1 (MQ=60)
                                                                                                               CCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAC                                     >  SRR19973069.1635652/1‑150 (MQ=60)
                                                                                                               CCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAC                                     >  SRR19973069.644903/1‑150 (MQ=60)
                                                                                                                 TGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCA                                   <  SRR19973069.3145251/150‑1 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                >  SRR19973069.146274/1‑150 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                <  SRR19973069.234450/150‑1 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                >  SRR19973069.146322/1‑150 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                <  SRR19973069.3562530/150‑1 (MQ=60)
                                                                                                                    AAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTT                                <  SRR19973069.3562522/150‑1 (MQ=60)
                                                                                                                     AATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTA                               >  SRR19973069.1233831/1‑150 (MQ=60)
                                                                                                                      ATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTAC                              >  SRR19973069.1587968/1‑150 (MQ=60)
                                                                                                                            AGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTT                        >  SRR19973069.2400297/1‑150 (MQ=60)
                                                                                                                            AGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTT                        >  SRR19973069.797782/1‑150 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973069.1134594/1‑150 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973069.471897/1‑150 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973069.480185/1‑150 (MQ=60)
                                                                                                                                    ATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATAACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCAC                >  SRR19973069.3142160/1‑150 (MQ=60)
                                                                                                                                     TATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACG               >  SRR19973069.820980/1‑150 (MQ=60)
                                                                                                                                       TGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAA             >  SRR19973069.3348602/1‑150 (MQ=60)
                                                                                                                                        GCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCGTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAAC            <  SRR19973069.2111080/150‑1 (MQ=60)
                                                                                                                                         CTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACA           >  SRR19973069.2847801/1‑150 (MQ=60)
                                                                                                                                          TCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACAT          <  SRR19973069.2316487/150‑1 (MQ=60)
                                                                                                                                          TCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACAT          <  SRR19973069.2315773/150‑1 (MQ=60)
                                                                                                                                             CTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCATGTTAGAACCACGAACATGAA       >  SRR19973069.3833621/1‑150 (MQ=60)
                                                                                                                                              TGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAAT      >  SRR19973069.1284588/1‑150 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     <  SRR19973069.750687/150‑1 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     <  SRR19973069.750961/150‑1 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     <  SRR19973069.1147730/150‑1 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     >  SRR19973069.1136283/1‑150 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATAGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     >  SRR19973069.1136776/1‑150 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTAGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATG     >  SRR19973069.1131426/1‑150 (MQ=60)
                                                                                                                                               GACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCGTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAATTACCAGGTTAGCACCAACAACATGAATG     >  SRR19973069.1136769/1‑150 (MQ=60)
                                                                                                                                                 CGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTT   <  SRR19973069.425180/150‑1 (MQ=60)
                                                                                                                                                  GGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  SRR19973069.2514892/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTC  >  CP009273/3860179‑3860474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑