Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,860,327 C→A 48.8% intergenic (‑70/+42) ibpB ← / ← ibpA heat shock chaperone/heat shock chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,860,3270CA48.8% 118.0 / inf 281intergenic (‑70/+42)ibpB/ibpAheat shock chaperone/heat shock chaperone
Reads supporting (aligned to +/- strand):  ref base C (77/67);  new base A (75/62);  total (152/129)
Fisher's exact test for biased strand distribution p-value = 9.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 284 total aligned reads displayed.

CCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTC  >  CP009273/3860178‑3860476
                                                                                                                                                     |                                                                                                                                                     
     gTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCAGAAGATTCGCATATGCTCCCAGACGGCGAGCa                                                                                                                                                  >  1:875349/1‑150 (MQ=255)
      ttttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCAt                                                                                                                                                 >  1:1651950/1‑150 (MQ=255)
       tttGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATg                                                                                                                                                >  1:185665/1‑150 (MQ=255)
                cGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCa                                                                                                                                       >  1:1113193/1‑150 (MQ=255)
                  ttGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAgg                                                                                                                                     >  2:3708582/1‑150 (MQ=255)
                  ttGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAgg                                                                                                                                     >  2:288938/1‑150 (MQ=255)
                   tGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGc                                                                                                                                    >  2:3918713/1‑150 (MQ=255)
                    ggCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGcc                                                                                                                                   <  2:4628554/150‑1 (MQ=255)
                           ttGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAg                                                                                                                            <  2:3986033/150‑1 (MQ=255)
                                 aaaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGcc                                                                                                                      >  1:3890830/1‑150 (MQ=255)
                                  aaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCt                                                                                                                     <  2:644/150‑1 (MQ=255)
                                  aaCCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCt                                                                                                                     >  1:441579/1‑150 (MQ=255)
                                    ccGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTa                                                                                                                   <  1:1711408/150‑1 (MQ=255)
                                       aTCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAggg                                                                                                                >  2:199903/1‑150 (MQ=255)
                                        tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGa                                                                                                               <  1:146383/150‑1 (MQ=255)
                                        tCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGa                                                                                                               >  2:3305149/1‑150 (MQ=255)
                                         ccATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAATACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGaa                                                                                                              >  1:4112862/1‑150 (MQ=255)
                                          cATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAt                                                                                                             <  1:4746855/150‑1 (MQ=255)
                                           aTTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAAtt                                                                                                            <  1:2554737/150‑1 (MQ=255)
                                                cGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTg                                                                                                       <  1:4691019/150‑1 (MQ=255)
                                                  cATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAt                                                                                                     <  2:2955727/150‑1 (MQ=255)
                                                  cATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAt                                                                                                     <  2:2704460/150‑1 (MQ=255)
                                                   aTCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAtt                                                                                                    <  2:1765728/150‑1 (MQ=255)
                                                   aTCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAtt                                                                                                    >  1:1617444/1‑150 (MQ=255)
                                                   aTCAGTGGGGATAAATCGAAGTTACGCAGAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGAtt                                                                                                    <  2:326347/150‑1 (MQ=255)
                                                     cAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTc                                                                                                  >  2:1636609/1‑150 (MQ=255)
                                                       gTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGa                                                                                                <  2:3129803/150‑1 (MQ=255)
                                                           ggATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACg                                                                                            <  1:4422162/150‑1 (MQ=255)
                                                              tAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGgcg                                                                                         >  2:3593091/1‑150 (MQ=255)
                                                                aaTCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGcgcg                                                                                       >  2:1105968/1‑150 (MQ=255)
                                                                  tCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGt                                                                                     <  1:227004/150‑1 (MQ=255)
                                                                   cGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtt                                                                                    <  2:1496626/150‑1 (MQ=255)
                                                                   cGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtt                                                                                    >  2:2245300/1‑150 (MQ=255)
                                                                     aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                                  >  2:3885542/1‑150 (MQ=255)
                                                                     aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                                  >  2:4682520/1‑150 (MQ=255)
                                                                     aaGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttt                                                                                  >  1:1425296/1‑150 (MQ=255)
                                                                       gTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTTAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGtttttt                                                                                <  1:4707549/150‑1 (MQ=255)
                                                                         tACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCg                                                                              >  1:797613/1‑150 (MQ=255)
                                                                         tACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCg                                                                              >  1:2954372/1‑150 (MQ=255)
                                                                            gCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCtt                                                                           <  2:3997488/150‑1 (MQ=255)
                                                                             cATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTc                                                                          <  1:2695727/150‑1 (MQ=255)
                                                                             cATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTc                                                                          >  1:1076233/1‑150 (MQ=255)
                                                                             cATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTc                                                                          >  2:2402463/1‑150 (MQ=255)
                                                                                aGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCgg                                                                       >  2:4649906/1‑150 (MQ=255)
                                                                                    aTTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGTGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATc                                                                   <  2:4578071/150‑1 (MQ=255)
                                                                                     tttCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGCAGTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCa                                                                  >  2:4242609/1‑150 (MQ=255)
                                                                                     tttCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCa                                                                  >  2:1839672/1‑150 (MQ=255)
                                                                                     tttCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCa                                                                  >  1:3044838/1‑150 (MQ=255)
                                                                                       tctcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcg                                                                <  1:668021/150‑1 (MQ=255)
                                                                                        ctcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgc                                                               >  1:4756411/1‑150 (MQ=255)
                                                                                        ctcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgc                                                               >  1:4260381/1‑150 (MQ=255)
                                                                                        ctcCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCAcgc                                                               <  2:1286553/150‑1 (MQ=255)
                                                                                           cTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGtt                                                            >  1:1017562/1‑150 (MQ=255)
                                                                                           cTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGtt                                                            <  2:1652675/150‑1 (MQ=255)
                                                                                               tAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgag                                                        >  2:3644359/1‑150 (MQ=255)
                                                                                               tAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCgag                                                        >  2:1777427/1‑150 (MQ=255)
                                                                                                 agaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAt                                                      <  2:1617444/150‑1 (MQ=255)
                                                                                                 agaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGAt                                                      <  2:2112761/150‑1 (MQ=255)
                                                                                                 agaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAGGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTGTTCGCTACCGGAATCACGCGGTCGAGAt                                                      >  1:2066439/1‑150 (MQ=255)
                                                                                                  gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATc                                                     <  1:2385949/150‑1 (MQ=255)
                                                                                                  gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATc                                                     >  1:347205/1‑150 (MQ=255)
                                                                                                  gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATc                                                     <  1:3663304/150‑1 (MQ=255)
                                                                                                  gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATc                                                     <  2:4291983/150‑1 (MQ=255)
                                                                                                  gaagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATc                                                     >  1:3948313/1‑150 (MQ=255)
                                                                                                   aagCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCg                                                    >  2:3950733/1‑150 (MQ=255)
                                                                                                    agCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGa                                                   <  1:1938638/150‑1 (MQ=255)
                                                                                                    agCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGa                                                   >  1:3528176/1‑150 (MQ=255)
                                                                                                    agCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGa                                                   >  1:2482136/1‑150 (MQ=255)
                                                                                                     gCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGat                                                  <  1:145548/150‑1 (MQ=255)
                                                                                                      cGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGata                                                 <  2:441579/150‑1 (MQ=255)
                                                                                                      cGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGata                                                 >  1:510230/1‑150 (MQ=255)
                                                                                                      cGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCGTCCGGCATCACGCGTTCGAGATCGata                                                 >  1:2899719/1‑150 (MQ=255)
                                                                                                        agtaagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCCGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                               >  2:1223119/1‑150 (MQ=255)
                                                                                                        agtaagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                               <  1:3382154/150‑1 (MQ=255)
                                                                                                        agtaagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                               >  2:3396938/1‑150 (MQ=255)
                                                                                                        agtaagtaCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGAtata                                               <  1:2818070/150‑1 (MQ=255)
                                                                                                               aCCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCaaa                                        <  1:380456/150‑1 (MQ=255)
                                                                                                                ccTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAc                                       <  2:3010636/150‑1 (MQ=255)
                                                                                                                 cTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAAcc                                      <  1:392546/150‑1 (MQ=255)
                                                                                                                  tGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCa                                     <  2:2610563/150‑1 (MQ=255)
                                                                                                                   gCAAATCCGAAGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCAt                                    >  1:4365973/1‑150 (MQ=255)
                                                                                                                      aaTCCGAAGATTCGCATATGCTCCCTGACGGAGAGCAGGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGACTTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTa                                 >  2:1026442/1‑150 (MQ=255)
                                                                                                                           gAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCGTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAgg                            >  1:4203246/1‑150 (MQ=255)
                                                                                                                           gAAGATTCGCATATGCTCCCTGACGGCGAGCAGGGAGAGGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGACTCACGCGTTCGAGATCGATATACAGCAAACCATTGACCAgg                            >  2:575091/1‑150 (MQ=255)
                                                                                                                            aaGATTCGCATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGt                           <  1:1507667/150‑1 (MQ=255)
                                                                                                                             aGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGtt                          >  1:1447232/1‑150 (MQ=255)
                                                                                                                              gATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTa                         <  2:2736000/150‑1 (MQ=255)
                                                                                                                                    cATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcacca                   >  1:3188674/1‑150 (MQ=255)
                                                                                                                                    cATATGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcacca                   <  2:1425661/150‑1 (MQ=255)
                                                                                                                                    cATATGCTCCCTGACGGAGAGCATGGAGAGGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcacca                   >  1:686649/1‑150 (MQ=255)
                                                                                                                                     atatGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGcaccac                  <  1:3585153/150‑1 (MQ=255)
                                                                                                                                       atGCTCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGa                >  2:87407/1‑150 (MQ=255)
                                                                                                                                        tGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGaa               >  1:3459140/1‑150 (MQ=255)
                                                                                                                                           tCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACAt            <  1:2743106/150‑1 (MQ=255)
                                                                                                                                           tCCCTGACGGAGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACAt            <  2:2430222/150‑1 (MQ=255)
                                                                                                                                            cccTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATg           <  1:1818979/150‑1 (MQ=255)
                                                                                                                                            cccTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATg           <  1:1819299/150‑1 (MQ=255)
                                                                                                                                              cTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGaa         >  2:535364/1‑150 (MQ=255)
                                                                                                                                                gACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATg       >  1:2347776/1‑150 (MQ=255)
                                                                                                                                                     cGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTtctc  >  1:1280462/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTCGCATATGCTCCCTGACGGCGAGCATGGAGATGTCAGGCCGCGCCAGGCGGCCTTAGGGAATTAGTTGATTTCGATACGGCGCGGTTTTTTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTC  >  CP009273/3860178‑3860476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A