Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 664,243 C→A 19.7% D84Y (GAT→TAT)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273664,2430CA19.7% 651.0 / 104.7 231D84Y (GAT→TAT) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (100/85);  new base A (16/30);  total (116/115)
Fisher's exact test for biased strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

ACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGG  >  CP009273/664094‑664392
                                                                                                                                                     |                                                                                                                                                     
 cgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCg                                                                                                                                                      <  1:4489518/150‑1 (MQ=255)
    cgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGccc                                                                                                                                                   <  2:4266488/150‑1 (MQ=255)
     gCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCa                                                                                                                                                  >  1:3825038/1‑150 (MQ=255)
     gCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCa                                                                                                                                                  >  1:3825184/1‑150 (MQ=255)
     gCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCa                                                                                                                                                  >  1:1563832/1‑150 (MQ=255)
     gCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCa                                                                                                                                                  >  1:3825102/1‑150 (MQ=255)
       aGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                                                                                                                                >  2:1495939/1‑150 (MQ=255)
          tACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTATTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACGTGGACAATCACATAGCCCAAATcc                                                                                                                                             <  2:2013973/150‑1 (MQ=255)
           aCTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCa                                                                                                                                            >  2:2476088/1‑150 (MQ=255)
           aCTCGGTAAAACCGGTTTGTACCCAGTCCGGCAATTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                                                                            >  1:239155/1‑150 (MQ=255)
            cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                                                                           <  1:790002/150‑1 (MQ=255)
            cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCAc                                                                                                                                           >  1:657905/1‑150 (MQ=255)
            cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCAc                                                                                                                                           >  1:2168711/1‑150 (MQ=255)
               ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                                                                                                                        >  1:1590107/1‑150 (MQ=255)
                 tAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCGTGACATGGACAATCACATCGCCCAAATCCACGACaa                                                                                                                                      >  2:227028/1‑150 (MQ=255)
                 tAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACaa                                                                                                                                      >  2:224786/1‑150 (MQ=255)
                       cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                                                                                <  2:3033083/150‑1 (MQ=255)
                       cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                                                                                >  1:2145544/1‑150 (MQ=255)
                       cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                                                                                >  1:2545843/1‑150 (MQ=255)
                            tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                                                                                                                           <  1:709773/150‑1 (MQ=255)
                                cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                                                                                                                       <  1:2632496/150‑1 (MQ=255)
                                cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                                                                                                                       <  1:1338542/150‑1 (MQ=255)
                                 ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                                                                                                                      <  2:2087237/150‑1 (MQ=255)
                                 ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                                                                                                                      >  1:994812/1‑150 (MQ=255)
                                         gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt                                                                                                              >  1:805647/1‑150 (MQ=255)
                                         gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt                                                                                                              >  1:805744/1‑150 (MQ=255)
                                          cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                                                                             >  1:3424253/1‑150 (MQ=255)
                                          cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                                                                             >  2:4031691/1‑150 (MQ=255)
                                            ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa                                                                                                           <  2:4358685/150‑1 (MQ=255)
                                            ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa                                                                                                           <  2:3007233/150‑1 (MQ=255)
                                             tttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAc                                                                                                          >  1:2091807/1‑150 (MQ=255)
                                              ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                                                                                         <  2:1694554/150‑1 (MQ=255)
                                                     ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCg                                                                                                  <  1:2468357/150‑1 (MQ=255)
                                                     acACGGCGACAAGTTGCAGCTTCACTCATTAACTCCAGAGTTTTTACAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCg                                                                                                  <  2:3300136/149‑1 (MQ=255)
                                                        cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTa                                                                                               >  2:1120469/1‑150 (MQ=255)
                                                         ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                                                                              <  1:3781253/150‑1 (MQ=255)
                                                         ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                                                                              <  2:2074212/150‑1 (MQ=255)
                                                          gCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAAc                                                                                             <  1:174635/150‑1 (MQ=255)
                                                          gCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAAc                                                                                             <  2:3767405/150‑1 (MQ=255)
                                                               aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGcc                                                                                        <  2:4108842/150‑1 (MQ=255)
                                                                aGTTGCAGCTTCACGCATTACCTCCAGAGTTTTTCCAGGTCATACAGGCGACGGCTCTCTTCCAGCATGACATGGACAATCACATAGCCCAAAGCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGccc                                                                                       <  2:1635099/150‑1 (MQ=255)
                                                                aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGccc                                                                                       <  1:2290007/150‑1 (MQ=255)
                                                                aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTAATACAGGCGACGGCTCTCTTCCTGCATGACATGGAAAATAACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGccc                                                                                       <  2:3893889/150‑1 (MQ=255)
                                                                 gTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCg                                                                                      <  1:502751/150‑1 (MQ=255)
                                                                   tGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCt                                                                                    >  1:3997317/1‑150 (MQ=255)
                                                                    gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTg                                                                                   >  2:4184186/1‑150 (MQ=255)
                                                                     cAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgc                                                                                  >  2:4222152/1‑150 (MQ=255)
                                                                       gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc                                                                                <  1:4134456/150‑1 (MQ=255)
                                                                         ttCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCga                                                                              <  1:4234376/150‑1 (MQ=255)
                                                                          tCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCgag                                                                             <  2:3278018/150‑1 (MQ=255)
                                                                            aCGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCAAGCATGACATGGACAATCACATCGCCCAAAGCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGAc                                                                           >  1:4442538/1‑150 (MQ=255)
                                                                                aTTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCt                                                                       <  1:658305/150‑1 (MQ=255)
                                                                                aTTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCt                                                                       >  1:4243058/1‑150 (MQ=255)
                                                                                aTTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCt                                                                       >  1:3143407/1‑150 (MQ=255)
                                                                                       ccAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAAc                                                                <  1:825301/150‑1 (MQ=255)
                                                                                         agagTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt                                                              >  2:2031572/1‑150 (MQ=255)
                                                                                         agagTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt                                                              >  2:2031472/1‑150 (MQ=255)
                                                                                          gagTTTTTCCAGTACATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCACATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTg                                                             <  2:1277268/150‑1 (MQ=255)
                                                                                              ttttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa                                                         <  2:381129/150‑1 (MQ=255)
                                                                                               tttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAg                                                        <  2:4170717/150‑1 (MQ=255)
                                                                                                   cAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAAt                                                    <  1:1385287/150‑1 (MQ=255)
                                                                                                    aGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg                                                   >  2:4471785/1‑150 (MQ=255)
                                                                                                    aGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg                                                   >  1:4102676/1‑150 (MQ=255)
                                                                                                     gTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATgg                                                  >  1:195489/1‑150 (MQ=255)
                                                                                                       tCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGAc                                                >  1:2883938/1‑150 (MQ=255)
                                                                                                       tCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGAc                                                <  1:3251044/150‑1 (MQ=255)
                                                                                                        cATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACa                                               >  1:3550504/1‑150 (MQ=255)
                                                                                                          tACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATa                                             >  1:1926336/1‑150 (MQ=255)
                                                                                                           aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATaa                                            >  2:1611707/1‑150 (MQ=255)
                                                                                                           aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATaa                                            >  2:3871946/1‑150 (MQ=255)
                                                                                                            cAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAAc                                           <  2:3255019/150‑1 (MQ=255)
                                                                                                            cAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAAc                                           <  1:3372068/150‑1 (MQ=255)
                                                                                                              ggCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACAt                                         <  2:2129808/150‑1 (MQ=255)
                                                                                                                 gACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGAc                                      <  2:1935001/150‑1 (MQ=255)
                                                                                                                 gACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGAc                                      <  2:1934974/150‑1 (MQ=255)
                                                                                                                   cGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACgg                                    <  1:1580601/150‑1 (MQ=255)
                                                                                                                    ggCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGc                                   >  1:1356188/1‑150 (MQ=255)
                                                                                                                     gCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCt                                  <  2:1070950/150‑1 (MQ=255)
                                                                                                                      ctctctTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTg                                 <  2:1926213/150‑1 (MQ=255)
                                                                                                                           ttCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGt                            <  1:4014832/150‑1 (MQ=255)
                                                                                                                               tGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAccc                        <  2:1438415/150‑1 (MQ=255)
                                                                                                                                gCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCg                       >  2:3887486/1‑150 (MQ=255)
                                                                                                                                 cATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGt                      >  2:2096527/1‑150 (MQ=255)
                                                                                                                                    gACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACa                   >  1:3586975/1‑150 (MQ=255)
                                                                                                                                     aCATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAg                  >  1:1019872/1‑150 (MQ=255)
                                                                                                                                       aTGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgat                <  2:528962/150‑1 (MQ=255)
                                                                                                                                       aTGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgat                <  2:1073645/150‑1 (MQ=255)
                                                                                                                                        tGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatg               <  1:2673475/150‑1 (MQ=255)
                                                                                                                                          gACAATCACATCGCCCAAATCCACGACAATCCCGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatgat             <  1:4503011/150‑1 (MQ=255)
                                                                                                                                          gACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatgat             >  1:2949394/1‑150 (MQ=255)
                                                                                                                                          gACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatgat             <  1:633857/150‑1 (MQ=255)
                                                                                                                                           aCAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATc            >  1:3649309/1‑150 (MQ=255)
                                                                                                                                            cAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCa           >  2:2863008/1‑150 (MQ=255)
                                                                                                                                            cAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCa           >  1:4021171/1‑150 (MQ=255)
                                                                                                                                             aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCAt          >  1:1164741/1‑150 (MQ=255)
                                                                                                                                             aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCAt          >  1:1163945/1‑150 (MQ=255)
                                                                                                                                             aaTCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCAt          <  1:3659027/150‑1 (MQ=255)
                                                                                                                                               tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGc        <  2:2371710/150‑1 (MQ=255)
                                                                                                                                               tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGc        <  2:2370723/150‑1 (MQ=255)
                                                                                                                                               tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGc        >  1:2803871/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGG  >  CP009273/664094‑664392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A