Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 664,243 C→A 27.1% D84Y (GAT→TAT)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273664,2430CA27.1% 717.8 / 219.4 318D84Y (GAT→TAT) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (128/103);  new base A (43/44);  total (171/147)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

ACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCG  >  CP009273/664094‑664391
                                                                                                                                                     |                                                                                                                                                    
acgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATc                                                                                                                                                      <  2:896862/150‑1 (MQ=255)
acgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATc                                                                                                                                                      >  1:1930621/1‑150 (MQ=255)
 cgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCAGGACATGGACAATCACATCg                                                                                                                                                     >  2:3602270/1‑150 (MQ=255)
  gacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                                                                                                                    >  1:2652931/1‑150 (MQ=255)
      cAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa                                                                                                                                                >  1:4105161/1‑150 (MQ=255)
      cAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa                                                                                                                                                <  1:2431464/150‑1 (MQ=255)
       aGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTTCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCaaa                                                                                                                                               >  1:3989645/1‑150 (MQ=255)
            cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                                                                          <  1:2814131/150‑1 (MQ=255)
             tCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACg                                                                                                                                         <  2:2712639/150‑1 (MQ=255)
              cGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGa                                                                                                                                        >  1:1002599/1‑150 (MQ=255)
                  aaaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                                                                                                                    <  2:3977958/150‑1 (MQ=255)
                  aaaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                                                                                                                    >  2:640177/1‑150 (MQ=255)
                   aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                                                                                                                                   <  1:3535417/150‑1 (MQ=255)
                   aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                                                                                                                                   >  1:1398568/1‑150 (MQ=255)
                   aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATc                                                                                                                                   <  2:1131790/150‑1 (MQ=255)
                    aaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATcc                                                                                                                                  >  1:2361866/1‑150 (MQ=255)
                      ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg                                                                                                                                <  1:987632/150‑1 (MQ=255)
                       cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                                                                               <  1:2305362/150‑1 (MQ=255)
                         gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                                                                                                                             >  1:3804447/1‑150 (MQ=255)
                            tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                                                                                                                          >  1:4541730/1‑150 (MQ=255)
                            tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                                                                                                                          >  2:843332/1‑150 (MQ=255)
                            tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGgcg                                                                                                                          >  2:3004740/1‑150 (MQ=255)
                            tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGgcg                                                                                                                          >  1:3383192/1‑150 (MQ=255)
                                   aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt                                                                                                                   >  1:1111274/1‑150 (MQ=255)
                                     tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa                                                                                                                 >  2:3701974/1‑150 (MQ=255)
                                       cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc                                                                                                               >  1:4190152/1‑150 (MQ=255)
                                        ggCATTTTCGTTCCCACGGCGACAAGTTTCAGCTTCACTCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCTCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt                                                                                                              <  2:4932752/150‑1 (MQ=255)
                                        ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt                                                                                                              <  1:4387409/150‑1 (MQ=255)
                                          cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                                                                            >  1:1483486/1‑150 (MQ=255)
                                           aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt                                                                                                           >  1:2649908/1‑150 (MQ=255)
                                            ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa                                                                                                          >  2:2979361/1‑150 (MQ=255)
                                              ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                                                                                        >  2:2524041/1‑150 (MQ=255)
                                              ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCATCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                                                                                        >  2:4572962/1‑150 (MQ=255)
                                                cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc                                                                                                      <  1:1128481/150‑1 (MQ=255)
                                                 gTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg                                                                                                     >  2:1723431/1‑150 (MQ=255)
                                                  ttCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGa                                                                                                    >  1:3480941/1‑150 (MQ=255)
                                                   tCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAg                                                                                                   <  1:4923238/150‑1 (MQ=255)
                                                    cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc                                                                                                  >  1:4646103/1‑150 (MQ=255)
                                                      cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                                                                                        <  1:4847958/142‑1 (MQ=255)
                                                      cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCgg                                                                                                <  2:3352228/150‑1 (MQ=255)
                                                      cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCgg                                                                                                <  2:4170966/150‑1 (MQ=255)
                                                         ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                                                                             >  1:1870846/1‑150 (MQ=255)
                                                         ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                                                                             <  1:5030471/150‑1 (MQ=255)
                                                         ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                                                                             >  1:876680/1‑150 (MQ=255)
                                                             aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAgg                                                                                         <  1:2549292/150‑1 (MQ=255)
                                                             aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAgg                                                                                         <  2:4736934/150‑1 (MQ=255)
                                                              cAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGc                                                                                        >  2:558619/1‑150 (MQ=255)
                                                                    gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTg                                                                                  <  1:165562/150‑1 (MQ=255)
                                                                      aGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcg                                                                                <  1:120137/150‑1 (MQ=255)
                                                                             cGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACt                                                                         <  1:2079985/150‑1 (MQ=255)
                                                                                aTTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCt                                                                      >  2:1539777/1‑150 (MQ=255)
                                                                                 ttAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTg                                                                     >  1:4957335/1‑150 (MQ=255)
                                                                                        cAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACg                                                              <  1:3634488/150‑1 (MQ=255)
                                                                                              ttttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTAGTCa                                                        <  2:1799263/150‑1 (MQ=255)
                                                                                              ttttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa                                                        >  1:5118192/1‑150 (MQ=255)
                                                                                               tttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAg                                                       <  1:4246579/150‑1 (MQ=255)
                                                                                                ttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGc                                                      >  1:2972220/1‑150 (MQ=255)
                                                                                                ttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGc                                                      <  1:2399942/150‑1 (MQ=255)
                                                                                                   cAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAAt                                                   <  2:5008651/150‑1 (MQ=255)
                                                                                                    aGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg                                                  >  1:4460991/1‑150 (MQ=255)
                                                                                                      ttCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGa                                                <  2:406486/150‑1 (MQ=255)
                                                                                                      ttCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGa                                                <  1:3252167/150‑1 (MQ=255)
                                                                                                       tCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGAc                                               <  1:1985718/150‑1 (MQ=255)
                                                                                                        cATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACa                                              <  2:2771888/150‑1 (MQ=255)
                                                                                                          tACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATa                                            >  1:2922394/1‑150 (MQ=255)
                                                                                                          tACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATa                                            <  1:2035257/150‑1 (MQ=255)
                                                                                                           aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATaa                                           <  2:3806912/150‑1 (MQ=255)
                                                                                                           aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATaa                                           >  2:4813444/1‑150 (MQ=255)
                                                                                                           aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATaa                                           >  2:1486199/1‑150 (MQ=255)
                                                                                                             aGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACa                                         >  2:2171715/1‑150 (MQ=255)
                                                                                                              ggCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACAt                                        <  1:859614/150‑1 (MQ=255)
                                                                                                                  aCGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACg                                    <  1:572361/150‑1 (MQ=255)
                                                                                                                  aCGGCTCTCTTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACg                                    <  2:2209014/150‑1 (MQ=255)
                                                                                                                    ggCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGc                                  >  1:709211/1‑150 (MQ=255)
                                                                                                                       tctctTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTgg                               >  1:2908310/1‑150 (MQ=255)
                                                                                                                       tctctTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTgg                               >  1:3362031/1‑150 (MQ=255)
                                                                                                                          ctTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACg                            <  2:4315330/150‑1 (MQ=255)
                                                                                                                          ctTCCTGCATGACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACg                            >  2:1613310/1‑150 (MQ=255)
                                                                                                                           ttCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGt                           >  1:1634684/1‑150 (MQ=255)
                                                                                                                            tCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTa                          >  1:3332140/1‑150 (MQ=255)
                                                                                                                              cTGCATGACATGGACAATCACATCGCCCAACTCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTCCGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAcc                        <  2:2390015/150‑1 (MQ=255)
                                                                                                                               tGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAccc                       <  2:1275533/150‑1 (MQ=255)
                                                                                                                                gCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCg                      >  1:926191/1‑150 (MQ=255)
                                                                                                                                    gACATGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACa                  >  2:3160817/1‑150 (MQ=255)
                                                                                                                                      cATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAga                >  1:2738689/1‑150 (MQ=255)
                                                                                                                                       aTGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgat               <  2:4465285/150‑1 (MQ=255)
                                                                                                                                       aTGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgat               <  2:1911498/150‑1 (MQ=255)
                                                                                                                                        tGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatg              >  2:142912/1‑150 (MQ=255)
                                                                                                                                        tGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCTGTACAgatg              <  2:2235498/150‑1 (MQ=255)
                                                                                                                                        tGGACAATCACATAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatg              <  2:2298284/150‑1 (MQ=255)
                                                                                                                                            cAATCACATCGCCCAACTCCACGACACTCCAGTCGGCGCTGTTTTCACCTTCGACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCa          <  2:1031170/150‑1 (MQ=255)
                                                                                                                                             aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCAt         <  2:2906897/150‑1 (MQ=255)
                                                                                                                                              aTCACATCGCCCAGATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATg        <  2:2618244/150‑1 (MQ=255)
                                                                                                                                              aTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATg        >  1:3509857/1‑150 (MQ=255)
                                                                                                                                               tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGc       >  2:3696992/1‑150 (MQ=255)
                                                                                                                                                cacaTAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCa      >  2:758330/1‑150 (MQ=255)
                                                                                                                                                 acaTAGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAg     <  2:3245263/150‑1 (MQ=255)
                                                                                                                                                  caTCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGt    >  2:1943505/1‑150 (MQ=255)
                                                                                                                                                   aTCGCCCAACTCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTc   <  1:3043019/150‑1 (MQ=255)
                                                                                                                                                   aTCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTc   >  1:1913125/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCG  >  CP009273/664094‑664391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A