Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 745,420 C→A 44.0% G736C (GGT→TGT)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273745,4200CA44.0% 241.2 / inf 284G736C (GGT→TGT) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (81/78);  new base A (59/66);  total (140/144)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 293 total aligned reads displayed.

CAGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCG  >  CP009273/745271‑745569
                                                                                                                                                     |                                                                                                                                                     
        tttCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACggg                                                                                                                                               <  1:724391/150‑1 (MQ=255)
         ttCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGa                                                                                                                                              >  2:2190969/1‑150 (MQ=255)
         ttCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGa                                                                                                                                              >  1:4489256/1‑150 (MQ=255)
          tCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGaa                                                                                                                                             >  2:2062769/1‑150 (MQ=255)
           cAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAg                                                                                                                                            <  2:2962803/150‑1 (MQ=255)
           cAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAg                                                                                                                                            >  2:3585041/1‑150 (MQ=255)
            aGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGc                                                                                                                                           >  1:3595857/1‑150 (MQ=255)
              cGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCa                                                                                                                                         <  2:2296240/150‑1 (MQ=255)
                 cacCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTg                                                                                                                                      >  1:2382295/1‑150 (MQ=255)
                 cacCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTg                                                                                                                                      <  1:2239291/150‑1 (MQ=255)
                  acCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGa                                                                                                                                     >  1:151990/1‑150 (MQ=255)
                  acCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGa                                                                                                                                     <  2:3549835/150‑1 (MQ=255)
                    cGCTTAACCAGGCAGGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAGCTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGAtt                                                                                                                                   <  2:4267990/150‑1 (MQ=255)
                     gCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAttt                                                                                                                                  >  2:2030173/1‑150 (MQ=255)
                     gCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAttt                                                                                                                                  <  1:2016804/150‑1 (MQ=255)
                     gCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAttt                                                                                                                                  <  1:94890/150‑1 (MQ=255)
                      cTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTg                                                                                                                                 <  1:2988500/150‑1 (MQ=255)
                        tAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTa                                                                                                                               >  1:673622/1‑150 (MQ=255)
                        tAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTa                                                                                                                               >  2:1600845/1‑150 (MQ=255)
                          aCCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAAc                                                                                                                             >  1:1253216/1‑150 (MQ=255)
                              ggCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTAt                                                                                                                         >  2:663441/1‑150 (MQ=255)
                                 aTGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATcgc                                                                                                                      >  1:3774040/1‑150 (MQ=255)
                                     ccttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCt                                                                                                                  >  2:1159459/1‑150 (MQ=255)
                                     ccttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCt                                                                                                                  >  2:3070864/1‑150 (MQ=255)
                                       ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  2:4025156/150‑1 (MQ=255)
                                       ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  2:2033144/150‑1 (MQ=255)
                                       ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  1:1211295/150‑1 (MQ=255)
                                         cctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGACGCCAGTGATTTGTAACCTATCGCGCCTTCaa                                                                                                              >  2:108128/1‑150 (MQ=255)
                                          ctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAAt                                                                                                             >  2:1333807/1‑150 (MQ=255)
                                          ctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAAt                                                                                                             >  2:1332747/1‑150 (MQ=255)
                                                  ccATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGttt                                                                                                     <  1:1419711/150‑1 (MQ=255)
                                                   cATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTc                                                                                                    <  1:2750901/150‑1 (MQ=255)
                                                    aTACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTcc                                                                                                   <  1:1539418/150‑1 (MQ=255)
                                                     tACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCt                                                                                                  <  2:2349117/150‑1 (MQ=255)
                                                      aCCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCtt                                                                                                 <  1:793897/150‑1 (MQ=255)
                                                      aCCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCtt                                                                                                 <  1:184997/150‑1 (MQ=255)
                                                      aCCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCtt                                                                                                 >  2:3766428/1‑150 (MQ=255)
                                                       cccACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtg                                                                                                >  2:3844363/1‑150 (MQ=255)
                                                        ccACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtga                                                                                               <  2:1777317/150‑1 (MQ=255)
                                                         cACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgat                                                                                              <  2:1859701/150‑1 (MQ=255)
                                                           cGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgatga                                                                                            <  2:2420006/150‑1 (MQ=255)
                                                           cGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgatga                                                                                            >  2:11111/1‑150 (MQ=255)
                                                             cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                                          <  1:2848921/150‑1 (MQ=255)
                                                             cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                                          <  1:2848836/150‑1 (MQ=255)
                                                             cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                                          >  2:3934954/1‑150 (MQ=255)
                                                              tAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGt                                                                                         >  2:413922/1‑150 (MQ=255)
                                                               aaTGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGtt                                                                                        <  1:1464037/150‑1 (MQ=255)
                                                               aaTGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGtt                                                                                        <  1:3729369/150‑1 (MQ=255)
                                                                aTGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGttt                                                                                       <  2:1339394/150‑1 (MQ=255)
                                                                   ccGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCt                                                                                    >  1:390438/1‑150 (MQ=255)
                                                                     gCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGc                                                                                  <  2:4534993/150‑1 (MQ=255)
                                                                        gTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAAc                                                                               >  1:1627030/1‑150 (MQ=255)
                                                                         tCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              <  1:1710475/150‑1 (MQ=255)
                                                                         tCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              <  1:965052/150‑1 (MQ=255)
                                                                          cATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTg                                                                             <  2:4418558/150‑1 (MQ=255)
                                                                          cATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTg                                                                             <  1:1741733/150‑1 (MQ=255)
                                                                          cATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTg                                                                             <  2:2584668/150‑1 (MQ=255)
                                                                           aTCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGACCGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGAAACTGt                                                                            >  2:3242492/1‑150 (MQ=255)
                                                                           aTCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGt                                                                            >  1:1955570/1‑150 (MQ=255)
                                                                            tCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                                                           >  1:2865727/1‑150 (MQ=255)
                                                                             ccTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTa                                                                          <  1:885845/150‑1 (MQ=255)
                                                                              cTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTAc                                                                         <  1:1305294/150‑1 (MQ=255)
                                                                              cTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTAc                                                                         >  1:4328582/1‑150 (MQ=255)
                                                                               tGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACg                                                                        >  2:2993909/1‑150 (MQ=255)
                                                                                   ggATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGt                                                                    >  1:3240669/1‑150 (MQ=255)
                                                                                   ggATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGt                                                                    >  1:3239534/1‑150 (MQ=255)
                                                                                            cACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                                                           >  1:820782/1‑150 (MQ=255)
                                                                                            cACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                                                           >  1:820178/1‑150 (MQ=255)
                                                                                              ccGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtca                                                         <  2:2555719/150‑1 (MQ=255)
                                                                                              ccGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtca                                                         <  2:3372886/150‑1 (MQ=255)
                                                                                                gaggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcatt                                                       >  1:1919233/1‑150 (MQ=255)
                                                                                                  ggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcattca                                                     <  1:3116352/150‑1 (MQ=255)
                                                                                                  ggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcattca                                                     <  1:838058/150‑1 (MQ=255)
                                                                                                   gggagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCGATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcattcat                                                    <  2:4412355/150‑1 (MQ=255)
                                                                                                         gaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCAc                                              >  2:1466403/1‑150 (MQ=255)
                                                                                                            cTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGc                                           >  1:2651635/1‑150 (MQ=255)
                                                                                                                  gCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGt                                     <  2:2355381/150‑1 (MQ=255)
                                                                                                                   cTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTg                                    <  1:737960/150‑1 (MQ=255)
                                                                                                                    tGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTgg                                   >  1:2312199/1‑150 (MQ=255)
                                                                                                                       cGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAg                                <  1:1071330/150‑1 (MQ=255)
                                                                                                                        gTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTAACGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAgg                               <  1:3905839/150‑1 (MQ=255)
                                                                                                                        gTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAgg                               <  1:563610/150‑1 (MQ=255)
                                                                                                                          tGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCt                             >  2:2208342/1‑150 (MQ=255)
                                                                                                                              tATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTa                         <  2:1955570/150‑1 (MQ=255)
                                                                                                                              tATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTa                         >  2:852267/1‑150 (MQ=255)
                                                                                                                                tgatgaCGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAAc                       >  1:3107581/1‑150 (MQ=255)
                                                                                                                                      cGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGaaa                 <  1:3345937/150‑1 (MQ=255)
                                                                                                                                          aaCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCaa             >  2:3978418/1‑150 (MQ=255)
                                                                                                                                          aaCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCaa             >  1:1575625/1‑150 (MQ=255)
                                                                                                                                            cGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACg           <  2:2408416/150‑1 (MQ=255)
                                                                                                                                            cGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACg           >  1:1826421/1‑150 (MQ=255)
                                                                                                                                              tCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGc         <  2:4348511/150‑1 (MQ=255)
                                                                                                                                              tCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGc         >  1:4177581/1‑150 (MQ=255)
                                                                                                                                                tttACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGcac       <  1:1065576/150‑1 (MQ=255)
                                                                                                                                                 ttACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGcacc      >  1:4286624/1‑150 (MQ=255)
                                                                                                                                                  tACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGcacca     <  1:1697324/150‑1 (MQ=255)
                                                                                                                                                   aCCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGcaccac    >  2:350941/1‑150 (MQ=255)
                                                                                                                                                    ccGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCaccacc   <  2:37098/150‑1 (MQ=255)
                                                                                                                                                    ccGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCaccacc   <  2:3090848/150‑1 (MQ=255)
                                                                                                                                                     cGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCg  >  2:3133087/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCG  >  CP009273/745271‑745569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A