Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 745,420 C→A 14.9% G736C (GGT→TGT)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273745,4200CA14.9% 1000.0 / 105.0 307G736C (GGT→TGT) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (111/150);  new base A (31/15);  total (142/165)
Fisher's exact test for biased strand distribution p-value = 2.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 311 total aligned reads displayed.

CAGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCG  >  CP009273/745271‑745569
                                                                                                                                                     |                                                                                                                                                     
cAGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTAcc                                                                                                                                                       >  1:2198562/1‑150 (MQ=255)
 aGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCg                                                                                                                                                      <  1:1224557/150‑1 (MQ=255)
 aGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCg                                                                                                                                                      <  2:2931174/150‑1 (MQ=255)
   ttGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGa                                                                                                                                                    >  1:847469/1‑150 (MQ=255)
    tGATTTTCAGCGACACCGCTTTACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGaa                                                                                                                                                   <  2:1816533/150‑1 (MQ=255)
    tGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGaa                                                                                                                                                   <  1:1496929/150‑1 (MQ=255)
    tGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTACCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGaa                                                                                                                                                   >  2:1581406/1‑150 (MQ=255)
      aTTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACg                                                                                                                                                 >  1:3546937/1‑150 (MQ=255)
      aTTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACg                                                                                                                                                 >  2:4058430/1‑150 (MQ=255)
      aTTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACg                                                                                                                                                 <  2:3332689/150‑1 (MQ=255)
          tCAGCGACACCGGTTAAACAGGCATGTCCTTCCTCGCCAACCATACCCACGCTATTGCCGCTGTCATCCTGTCGTTTATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGaa                                                                                                                                             <  1:2285366/150‑1 (MQ=255)
          tCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGaa                                                                                                                                             >  1:1091518/1‑150 (MQ=255)
           cAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAg                                                                                                                                            <  2:2907169/150‑1 (MQ=255)
                  acCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGa                                                                                                                                     >  1:3209582/1‑150 (MQ=255)
                    cGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAtt                                                                                                                                   <  2:2583411/150‑1 (MQ=255)
                     gCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAttt                                                                                                                                  <  2:645589/150‑1 (MQ=255)
                     gCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGAttt                                                                                                                                  <  2:3108077/150‑1 (MQ=255)
                        tAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTa                                                                                                                               >  2:3867362/1‑150 (MQ=255)
                          aCCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAAc                                                                                                                             >  2:1737603/1‑150 (MQ=255)
                            cAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCt                                                                                                                           <  2:4346476/150‑1 (MQ=255)
                            cAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCt                                                                                                                           <  2:1119112/150‑1 (MQ=255)
                            cAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCt                                                                                                                           <  2:2447577/150‑1 (MQ=255)
                             aGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTa                                                                                                                          >  2:1363841/1‑150 (MQ=255)
                             aGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTa                                                                                                                          <  1:1384739/150‑1 (MQ=255)
                             aGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTa                                                                                                                          >  2:2407534/1‑150 (MQ=255)
                              ggCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTAt                                                                                                                         >  1:2426041/1‑150 (MQ=255)
                               gCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATc                                                                                                                        <  1:1842671/150‑1 (MQ=255)
                                  tGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATcgcg                                                                                                                     >  2:3129904/1‑150 (MQ=255)
                                   gTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCgcgc                                                                                                                    >  2:34772/1‑150 (MQ=255)
                                    tccttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACTGGAAGCCAGTGATTTGTAACCTATCGCGcc                                                                                                                   <  1:2377301/150‑1 (MQ=255)
                                     ccttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCt                                                                                                                  <  2:529710/150‑1 (MQ=255)
                                     ccttcatCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGTGAGGGAACTGACCGCTGTCGTTGCGTATGATGACGTTAACGTCTTAACCTGAACGGGAAGCCAGTGATTAGTAACCTATCGCGcca                                                                                                                  >  1:2659258/1‑149 (MQ=255)
                                       ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  2:165149/150‑1 (MQ=255)
                                       ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  2:212535/150‑1 (MQ=255)
                                       ttcctCGCCAACCAGACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTc                                                                                                                <  2:2052665/150‑1 (MQ=255)
                                        tcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCa                                                                                                               >  2:318068/1‑150 (MQ=255)
                                         cctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCaa                                                                                                              <  1:3650213/150‑1 (MQ=255)
                                             gCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCa                                                                                                          <  2:2218322/150‑1 (MQ=255)
                                              ccAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCAt                                                                                                         <  2:2464552/150‑1 (MQ=255)
                                               cAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATg                                                                                                        >  1:332069/1‑150 (MQ=255)
                                                aaCCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGt                                                                                                       <  1:516565/150‑1 (MQ=255)
                                                aaCCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGt                                                                                                       <  1:338661/150‑1 (MQ=255)
                                                  ccATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGttt                                                                                                     <  2:2215413/150‑1 (MQ=255)
                                                  ccATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGttt                                                                                                     >  2:808523/1‑150 (MQ=255)
                                                    aTACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTcc                                                                                                   >  2:1267518/1‑150 (MQ=255)
                                                     tACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCt                                                                                                  >  1:2157864/1‑150 (MQ=255)
                                                      aCCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCtt                                                                                                 <  2:3224255/150‑1 (MQ=255)
                                                         cACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgat                                                                                              <  1:1951486/150‑1 (MQ=255)
                                                         cACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgat                                                                                              <  2:1456802/150‑1 (MQ=255)
                                                          aCGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTtgatg                                                                                             <  2:600302/150‑1 (MQ=255)
                                                             cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                                          >  1:952719/1‑150 (MQ=255)
                                                             cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                                          <  2:3768983/150‑1 (MQ=255)
                                                              tAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGt                                                                                         >  2:231000/1‑150 (MQ=255)
                                                                 tGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGtttt                                                                                      >  1:799375/1‑150 (MQ=255)
                                                                  gCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTc                                                                                     <  1:1292417/150‑1 (MQ=255)
                                                                  gCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTc                                                                                     <  2:1020270/150‑1 (MQ=255)
                                                                   ccGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCt                                                                                    <  2:2773633/150‑1 (MQ=255)
                                                                    cGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTg                                                                                   <  2:224580/150‑1 (MQ=255)
                                                                         tCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              >  2:2359466/1‑150 (MQ=255)
                                                                         tCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              <  2:1967085/150‑1 (MQ=255)
                                                                         tCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              >  1:854532/1‑150 (MQ=255)
                                                                         tCATCCTGGCGGAGATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACt                                                                              <  1:2887992/150‑1 (MQ=255)
                                                                          cATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTg                                                                             >  1:4012889/1‑150 (MQ=255)
                                                                          cATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTg                                                                             >  2:4133154/1‑150 (MQ=255)
                                                                           aTCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGt                                                                            <  1:4503466/150‑1 (MQ=255)
                                                                            tCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGTAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                                                           <  1:4200671/150‑1 (MQ=255)
                                                                            tCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                                                           <  1:3255275/150‑1 (MQ=255)
                                                                              cTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTAc                                                                         <  2:373512/150‑1 (MQ=255)
                                                                              cTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTAc                                                                         <  2:3922676/150‑1 (MQ=255)
                                                                               tGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACg                                                                        >  1:3702134/1‑150 (MQ=255)
                                                                                ggCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGc                                                                       >  1:3131431/1‑150 (MQ=255)
                                                                                  cGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCg                                                                     >  1:3583533/1‑150 (MQ=255)
                                                                                    gttttacGCTCCGATGGGAGTGAACTGACCGCTTGCTTTGCGTTTGATGTCGTTAACTTCTTTTCCGGTACGGGAAGGCAGTGATTTGTAACCTATCGCGCGTTCAATCCTTGTTTCCTTGTTGACGTTTTCTGCAACTGTTACGCCGTc                                                                   <  1:480705/144‑1 (MQ=255)
                                                                                       atCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCggg                                                                >  1:4470159/1‑150 (MQ=255)
                                                                                         ccGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTa                                                              <  2:4210530/150‑1 (MQ=255)
                                                                                               cGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcat                                                        >  1:2784144/1‑150 (MQ=255)
                                                                                                gaggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcatt                                                       >  1:1569222/1‑150 (MQ=255)
                                                                                                 aggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcattc                                                      <  2:2445721/150‑1 (MQ=255)
                                                                                                   gggagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtcattcat                                                    >  2:3946559/1‑150 (MQ=255)
                                                                                                     gagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGt                                                  >  1:4300455/1‑150 (MQ=255)
                                                                                                     gagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGt                                                  >  1:670386/1‑150 (MQ=255)
                                                                                                         gaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCAc                                              <  1:772219/150‑1 (MQ=255)
                                                                                                             tGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGcc                                          >  1:2611722/1‑150 (MQ=255)
                                                                                                               aCCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACAGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCAc                                        <  2:4477203/150‑1 (MQ=255)
                                                                                                                  gCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGt                                     >  1:2969669/1‑150 (MQ=255)
                                                                                                                  gCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGt                                     >  2:729979/1‑150 (MQ=255)
                                                                                                                  gCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGt                                     <  1:1594770/150‑1 (MQ=255)
                                                                                                                   cTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTg                                    <  2:1934360/150‑1 (MQ=255)
                                                                                                                    tGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTgg                                   >  1:3907820/1‑150 (MQ=255)
                                                                                                                             gTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGt                          <  2:4543536/150‑1 (MQ=255)
                                                                                                                             gTATGAGGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTACCCTCTCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGt                          <  2:273546/150‑1 (MQ=255)
                                                                                                                               atgatgACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTaa                        <  2:117690/150‑1 (MQ=255)
                                                                                                                                tgatgaCGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAAc                       <  1:1986800/150‑1 (MQ=255)
                                                                                                                                      cGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGaaa                 >  1:3592566/1‑150 (MQ=255)
                                                                                                                                      cGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGaaa                 <  1:3852581/150‑1 (MQ=255)
                                                                                                                                       gTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAAt                <  2:3091628/150‑1 (MQ=255)
                                                                                                                                       gTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAAt                >  2:3393591/1‑150 (MQ=255)
                                                                                                                                        ttAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATc               >  1:3758992/1‑150 (MQ=255)
                                                                                                                                          aaCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCaa             <  2:2644916/150‑1 (MQ=255)
                                                                                                                                          aaCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCaa             >  1:2544121/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGTTGATTTTCAGCGACACCGCTTAACCAGGCATGTCCTTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCG  >  CP009273/745271‑745569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A