Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,447 G→A 91.7% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,4470GA91.7% 773.9 / 45.8 241D89N (GAT→AAT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base A (96/124);  total (107/134)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 241 total aligned reads displayed.

CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTG  >  CP009273/876300‑876592
                                                                                                                                                   |                                                                                                                                                 
cgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGATTCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGat                                                                                                                                                 <  1:862218/150‑1 (MQ=255)
cgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGat                                                                                                                                                 <  1:862310/150‑1 (MQ=255)
 gTGCATGGATTTTAAGGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                                                                                >  2:208019/1‑150 (MQ=255)
      tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                                                                           <  1:1044998/150‑1 (MQ=255)
      tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                                                                           >  2:1528227/1‑150 (MQ=255)
          ttttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcgg                                                                                                                                       <  1:24924/150‑1 (MQ=255)
          ttttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcgg                                                                                                                                       <  2:3719566/150‑1 (MQ=255)
            ttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtc                                                                                                                                     <  2:2591945/150‑1 (MQ=255)
             tAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcg                                                                                                                                    >  2:1414537/1‑150 (MQ=255)
              aaTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcgg                                                                                                                                   >  1:4336985/1‑150 (MQ=255)
              aaTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcgg                                                                                                                                   <  2:4325919/150‑1 (MQ=255)
                tGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTa                                                                                                                                 <  2:2892899/150‑1 (MQ=255)
                tGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTa                                                                                                                                 <  1:2499445/150‑1 (MQ=255)
                 ggATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                                >  2:2052629/1‑150 (MQ=255)
                 ggATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                                <  1:3201018/150‑1 (MQ=255)
                   aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAg                                                                                                                              >  1:1233359/1‑150 (MQ=255)
                    ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGa                                                                                                                             >  1:4538546/1‑150 (MQ=255)
                    ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGa                                                                                                                             >  2:2549802/1‑150 (MQ=255)
                     tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAt                                                                                                                            >  1:345830/1‑150 (MQ=255)
                        gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGcc                                                                                                                         >  2:3838470/1‑150 (MQ=255)
                        gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATgtc                                                                                                                         <  1:2286177/150‑3 (MQ=255)
                          cAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTg                                                                                                                       <  2:1504735/150‑1 (MQ=255)
                              ggTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCg                                                                                                                   <  1:3364/150‑1 (MQ=255)
                               gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                  >  2:1891355/1‑150 (MQ=255)
                               gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                  <  1:3292245/150‑1 (MQ=255)
                               gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                  >  2:1281518/1‑150 (MQ=255)
                                 aaaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACg                                                                                                                >  1:2345015/1‑150 (MQ=255)
                                 aaaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACg                                                                                                                >  2:16151/1‑150 (MQ=255)
                                     tGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaa                                                                                                            >  1:1456166/1‑150 (MQ=255)
                                      gCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaaa                                                                                                           >  1:2263569/1‑150 (MQ=255)
                                        tGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATc                                                                                                         >  1:4083656/1‑150 (MQ=255)
                                          gCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCg                                                                                                       <  2:2294913/150‑1 (MQ=255)
                                               aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                                                                                  <  2:1826490/150‑1 (MQ=255)
                                                 gCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGc                                                                                                >  2:392087/1‑150 (MQ=255)
                                                 gCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGc                                                                                                >  2:392332/1‑150 (MQ=255)
                                                  cAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                               >  1:946358/1‑150 (MQ=255)
                                                  cAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                               >  1:1413733/1‑150 (MQ=255)
                                                      gCGGATGAGACACTGGATCCCGCGAGCCTGACTAACATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTACACACACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGt                                                                                           <  2:4039222/150‑1 (MQ=255)
                                                       cGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                                                                          >  2:29763/1‑150 (MQ=255)
                                                          aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                                                                       <  1:662914/150‑1 (MQ=255)
                                                              gaAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGt                                                                                   <  2:880971/150‑1 (MQ=255)
                                                                aaCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                                                                                 >  1:3166072/1‑150 (MQ=255)
                                                                 aCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt                                                                                <  1:2030430/150‑1 (MQ=255)
                                                                    ggttCCCGCGAGCCTGACTAAAATCATGACCAGCGTTGTGGTTGGTCAGGCGCTTAAGGCCGATAAGATTTAACTCAGCAATATGGTGACGGTCGGTAAAGATGCCTGGTCGAAGGGAAATCCGGCAATGCGTGGTTCATCGGTAATGtt                                                                             <  2:1755963/147‑1 (MQ=255)
                                                                    ggATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGtt                                                                             >  2:1988206/1‑150 (MQ=255)
                                                                      aTCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTcc                                                                           >  1:2796125/1‑150 (MQ=255)
                                                                       tCCCGCGAGCCTGACTACACTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                                                                          <  2:4171289/150‑1 (MQ=255)
                                                                       tCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                                                                          <  1:4319157/150‑1 (MQ=255)
                                                                        cccGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                                                                         >  2:1300819/1‑150 (MQ=255)
                                                                         ccGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCa                                                                        <  1:841817/150‑1 (MQ=255)
                                                                          cgcgAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                                                                       >  2:3307271/1‑150 (MQ=255)
                                                                          cgcgAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                                                                       >  2:3307250/1‑150 (MQ=255)
                                                                          cgcgAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                                                                       <  2:3825725/150‑1 (MQ=255)
                                                                           gcgAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaaa                                                                      <  1:3121648/150‑1 (MQ=255)
                                                                            cgAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAc                                                                     <  1:1104807/150‑1 (MQ=255)
                                                                             gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                    >  2:140129/1‑150 (MQ=255)
                                                                             gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                    >  2:894759/1‑150 (MQ=255)
                                                                              aGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                                                                   <  1:1197198/150‑1 (MQ=255)
                                                                              aGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                                                                   >  2:3152719/1‑150 (MQ=255)
                                                                               gCCTGACTAAAATCATGACCAGCTATGTGGGTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCgg                                                                  >  2:3451154/1‑150 (MQ=255)
                                                                                ccTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                 <  2:2274144/150‑1 (MQ=255)
                                                                                ccTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                 <  2:3590581/150‑1 (MQ=255)
                                                                                  tGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCg                                                               >  1:1629758/1‑150 (MQ=255)
                                                                                   gACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                              >  1:93515/1‑150 (MQ=255)
                                                                                   gACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                              >  1:1434978/1‑150 (MQ=255)
                                                                                        aaaTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAgg                                                         >  1:1814069/1‑150 (MQ=255)
                                                                                          aTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGtt                                                       <  2:2751609/150‑1 (MQ=255)
                                                                                           tCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGttt                                                      <  2:2657366/150‑1 (MQ=255)
                                                                                            cATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                     <  2:4215245/150‑1 (MQ=255)
                                                                                            cATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                     <  2:3258488/150‑1 (MQ=255)
                                                                                              tGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCgg                                                   >  1:1113480/1‑150 (MQ=255)
                                                                                               gACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGGTTCGGt                                                  >  2:3354945/1‑150 (MQ=255)
                                                                                                aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTg                                                 >  2:2937722/1‑150 (MQ=255)
                                                                                                aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTg                                                 <  1:2994857/150‑1 (MQ=255)
                                                                                                aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTg                                                 <  2:4538546/150‑1 (MQ=255)
                                                                                                  cAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGc                                               <  2:861616/150‑1 (MQ=255)
                                                                                                    gCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAg                                             >  1:3145431/1‑150 (MQ=255)
                                                                                                      tATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAc                                           <  2:3707575/150‑1 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                          >  1:2971720/1‑150 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                          <  1:3682172/150‑1 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                          >  1:1759605/1‑150 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                          <  1:1878470/150‑1 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                          >  1:2550617/1‑150 (MQ=255)
                                                                                                          tgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGa                                       <  1:2549802/150‑1 (MQ=255)
                                                                                                           ggTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGaa                                      <  1:1926130/150‑1 (MQ=255)
                                                                                                               gggCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaaa                                  >  1:4198394/1‑150 (MQ=255)
                                                                                                                ggCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAg                                 >  2:3045537/1‑150 (MQ=255)
                                                                                                                    ggCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtgt                             <  2:2135362/150‑1 (MQ=255)
                                                                                                                     gcgcTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGtgtg                            <  1:2903621/150‑1 (MQ=255)
                                                                                                                      cgcTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                           >  2:444799/1‑150 (MQ=255)
                                                                                                                        cTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGAtt                         <  1:3415371/150‑1 (MQ=255)
                                                                                                                          tAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAt                       >  2:305835/1‑150 (MQ=255)
                                                                                                                             ggCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCa                    <  2:2542828/150‑1 (MQ=255)
                                                                                                                                cGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTc                 >  2:1140748/1‑150 (MQ=255)
                                                                                                                                    aaGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGt             >  1:3551814/1‑150 (MQ=255)
                                                                                                                                     aGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTa            <  2:2598991/150‑1 (MQ=255)
                                                                                                                                      gATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTaa           <  2:693880/150‑1 (MQ=255)
                                                                                                                                      gATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTaa           <  2:1456166/150‑1 (MQ=255)
                                                                                                                                              tCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCt   <  1:2052629/150‑1 (MQ=255)
                                                                                                                                               cACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTg  <  2:1165320/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTG  >  CP009273/876300‑876592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876300‑876596
                                                                                                                                                   |                                                                                                                                                     
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGATTCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863490/150‑1 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGATACTGGATCCCGCGAGCCTGACTAAAATCATGACAAGATATGTGGTTGGGCAGGCGCTTATGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863483/150‑1 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863600/150‑1 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863523/150‑1 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863553/150‑1 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGAT                                                                                                                                                     <  SRR19973076.863582/150‑1 (MQ=60)
     ATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGT                                                                                                                                                <  SRR19973076.1780196/150‑1 (MQ=60)
      TGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTG                                                                                                                                               <  SRR19973076.1046547/150‑1 (MQ=60)
      TGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTG                                                                                                                                               <  SRR19973076.3325209/150‑1 (MQ=60)
          TTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGG                                                                                                                                           <  SRR19973076.24982/150‑1 (MQ=60)
             TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCG                                                                                                                                        >  SRR19973076.2280024/1‑150 (MQ=60)
             TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCG                                                                                                                                        >  SRR19973076.4524627/1‑150 (MQ=60)
              AATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGG                                                                                                                                       >  SRR19973076.4343391/1‑150 (MQ=60)
              AATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGG                                                                                                                                       >  SRR19973076.1551986/1‑150 (MQ=60)
                TGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTA                                                                                                                                     <  SRR19973076.2503080/150‑1 (MQ=60)
                TGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTA                                                                                                                                     <  SRR19973076.2503033/150‑1 (MQ=60)
                 GGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAA                                                                                                                                    <  SRR19973076.3205721/150‑1 (MQ=60)
                 GGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAA                                                                                                                                    <  SRR19973076.3205680/150‑1 (MQ=60)
                 GGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAA                                                                                                                                    <  SRR19973076.3205661/150‑1 (MQ=60)
                  GATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAA                                                                                                                                   >  SRR19973076.3369464/1‑150 (MQ=60)
                  GATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAA                                                                                                                                   >  SRR19973076.3369504/1‑150 (MQ=60)
                  GATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAA                                                                                                                                   >  SRR19973076.1934255/1‑150 (MQ=60)
                   ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAG                                                                                                                                  >  SRR19973076.1235183/1‑150 (MQ=60)
                   ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAG                                                                                                                                  >  SRR19973076.1235827/1‑150 (MQ=60)
                    TTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGA                                                                                                                                 >  SRR19973076.4545278/1‑150 (MQ=60)
                     TACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAT                                                                                                                                >  SRR19973076.3795692/1‑150 (MQ=60)
                     TACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAT                                                                                                                                >  SRR19973076.346144/1‑150 (MQ=60)
                     TACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAT                                                                                                                                >  SRR19973076.346362/1‑150 (MQ=60)
                      ACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATG                                                                                                                               >  SRR19973076.676745/1‑150 (MQ=60)
                      ACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTAGTTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATG                                                                                                                               >  SRR19973076.4450920/1‑150 (MQ=60)
                        GCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGTC                                                                                                                             <  SRR19973076.2867480/150‑1 (MQ=60)
                        GCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGTC                                                                                                                             <  SRR19973076.2289453/150‑1 (MQ=60)
                          CAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTG                                                                                                                           <  SRR19973076.2423670/150‑1 (MQ=60)
                              GGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCG                                                                                                                       <  SRR19973076.3369/150‑1 (MQ=60)
                               GTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGA                                                                                                                      <  SRR19973076.3297025/150‑1 (MQ=60)
                                 AAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACG                                                                                                                    >  SRR19973076.2348388/1‑150 (MQ=60)
                                    GTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGA                                                                                                                 <  SRR19973076.3204350/150‑1 (MQ=60)
                                     TGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAA                                                                                                                >  SRR19973076.1458299/1‑150 (MQ=60)
                                      GCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAA                                                                                                               >  SRR19973076.2266813/1‑150 (MQ=60)
                                       CTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAT                                                                                                              >  SRR19973076.1167025/1‑150 (MQ=60)
                                       CTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAT                                                                                                              >  SRR19973076.1167044/1‑150 (MQ=60)
                                        TGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATC                                                                                                             >  SRR19973076.4089686/1‑150 (MQ=60)
                                                  CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                   >  SRR19973076.1415815/1‑150 (MQ=60)
                                                  CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                   >  SRR19973076.1415814/1‑150 (MQ=60)
                                                  CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                   >  SRR19973076.947747/1‑150 (MQ=60)
                                                  CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                   >  SRR19973076.947755/1‑150 (MQ=60)
                                                       CGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTT                                                                                              <  SRR19973076.2299222/150‑1 (MQ=60)
                                                         GATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCA                                                                                            >  SRR19973076.704463/1‑150 (MQ=60)
                                                          ATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAT                                                                                           <  SRR19973076.663909/150‑1 (MQ=60)
                                                              GAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGT                                                                                       >  SRR19973076.1774641/1‑150 (MQ=60)
                                                               AAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTA                                                                                      <  SRR19973076.2671451/150‑1 (MQ=60)
                                                                AACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAA                                                                                     >  SRR19973076.3170666/1‑150 (MQ=60)
                                                                AACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAA                                                                                     >  SRR19973076.3170645/1‑150 (MQ=60)
                                                                 ACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT                                                                                    <  SRR19973076.2033444/150‑1 (MQ=60)
                                                                 ACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT                                                                                    <  SRR19973076.2033402/150‑1 (MQ=60)
                                                                     GATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTC                                                                                >  SRR19973076.1090684/1‑150 (MQ=60)
                                                                      ATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCC                                                                               >  SRR19973076.2800188/1‑150 (MQ=60)
                                                                       TCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                                                                              <  SRR19973076.4325535/150‑1 (MQ=60)
                                                                       TCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                                                                              <  SRR19973076.220689/150‑1 (MQ=60)
                                                                       TCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                                                                              <  SRR19973076.279401/150‑1 (MQ=60)
                                                                        CCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                             <  SRR19973076.62858/150‑1 (MQ=60)
                                                                         CCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCA                                                                            <  SRR19973076.843050/150‑1 (MQ=60)
                                                                           GCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAA                                                                          <  SRR19973076.3126175/150‑1 (MQ=60)
                                                                           GCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAA                                                                          <  SRR19973076.3126257/150‑1 (MQ=60)
                                                                           GCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAA                                                                          <  SRR19973076.3126263/150‑1 (MQ=60)
                                                                            CGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAC                                                                         <  SRR19973076.1106443/150‑1 (MQ=60)
                                                                            CGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAC                                                                         <  SRR19973076.1106455/150‑1 (MQ=60)
                                                                              AGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCG                                                                       <  SRR19973076.1198977/150‑1 (MQ=60)
                                                                              AGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCG                                                                       <  SRR19973076.1201200/150‑1 (MQ=60)
                                                                                  TGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCG                                                                   >  SRR19973076.1632145/1‑150 (MQ=60)
                                                                                   GACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGA                                                                  >  SRR19973076.93701/1‑150 (MQ=60)
                                                                                   GACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGA                                                                  >  SRR19973076.1437105/1‑150 (MQ=60)
                                                                                   GACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGA                                                                  >  SRR19973076.1437083/1‑150 (MQ=60)
                                                                                   GACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGA                                                                  >  SRR19973076.1437016/1‑150 (MQ=60)
                                                                                       AAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAG                                                              <  SRR19973076.1513469/150‑1 (MQ=60)
                                                                                        AAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGG                                                             >  SRR19973076.1816678/1‑150 (MQ=60)
                                                                                              TGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGG                                                       >  SRR19973076.1115126/1‑150 (MQ=60)
                                                                                              TGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGG                                                       >  SRR19973076.3545443/1‑150 (MQ=60)
                                                                                               GACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGT                                                      >  SRR19973076.1693485/1‑150 (MQ=60)
                                                                                                ACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTG                                                     <  SRR19973076.2999215/150‑1 (MQ=60)
                                                                                                ACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTG                                                     <  SRR19973076.2999191/150‑1 (MQ=60)
                                                                                                ACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTG                                                     <  SRR19973076.2997981/150‑1 (MQ=60)
                                                                                                    GCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAG                                                 >  SRR19973076.3149995/1‑150 (MQ=60)
                                                                                                     CTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGA                                                >  SRR19973076.1829272/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973076.1762151/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              <  SRR19973076.1881173/150‑1 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973076.2976046/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973076.2974060/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973076.2554329/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973076.2554098/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              <  SRR19973076.3687549/150‑1 (MQ=60)
                                                                                                          TGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGA                                           <  SRR19973076.2553514/150‑1 (MQ=60)
                                                                                                           GGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAA                                          <  SRR19973076.1928895/150‑1 (MQ=60)
                                                                                                           GGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAA                                          <  SRR19973076.1928915/150‑1 (MQ=60)
                                                                                                           GGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAA                                          <  SRR19973076.1928919/150‑1 (MQ=60)
                                                                                                               GGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAA                                      >  SRR19973076.4204247/1‑150 (MQ=60)
                                                                                                               GGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAA                                      >  SRR19973076.4204600/1‑150 (MQ=60)
                                                                                                               GGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAA                                      >  SRR19973076.4204593/1‑150 (MQ=60)
                                                                                                               GGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAA                                      >  SRR19973076.4204333/1‑150 (MQ=60)
                                                                                                                 GCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGG                                    >  SRR19973076.2541407/1‑150 (MQ=60)
                                                                                                                     GCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTG                                <  SRR19973076.2907834/150‑1 (MQ=60)
                                                                                                                      CGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGA                               >  SRR19973076.2185218/1‑150 (MQ=60)
                                                                                                                        CTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATT                             <  SRR19973076.3613141/150‑1 (MQ=60)
                                                                                                                        CTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATT                             <  SRR19973076.3613548/150‑1 (MQ=60)
                                                                                                                        CTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATT                             <  SRR19973076.3420320/150‑1 (MQ=60)
                                                                                                                                    AAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGT                 >  SRR19973076.3556979/1‑150 (MQ=60)
                                                                                                                                      GATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAA               <  SRR19973076.235757/150‑1 (MQ=60)
                                                                                                                                      GATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAA               <  SRR19973076.2539940/150‑1 (MQ=60)
                                                                                                                                        TTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATG             <  SRR19973076.3002314/150‑1 (MQ=60)
                                                                                                                                          AAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGAC           >  SRR19973076.4329963/1‑150 (MQ=60)
                                                                                                                                              TCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCT       <  SRR19973076.2055623/150‑1 (MQ=60)
                                                                                                                                                   AATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  SRR19973076.3254716/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876300‑876596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑