Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,447 G→A 100% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,4470GA100.0% 828.1 / NA 253D89N (GAT→AAT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (122/131);  total (122/131)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 253 total aligned reads displayed.

CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGT  >  CP009273/876300‑876593
                                                                                                                                                   |                                                                                                                                                  
 gTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                                                                                 >  2:3836057/1‑150 (MQ=255)
  tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAatat                                                                                                                                                >  2:603245/1‑150 (MQ=255)
    cATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGTCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATgg                                                                                                                                              >  2:401465/1‑150 (MQ=255)
      tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                                                                            >  2:1964871/1‑150 (MQ=255)
        gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGAc                                                                                                                                          >  1:2647056/1‑150 (MQ=255)
        gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAc                                                                                                                                          <  1:346807/150‑1 (MQ=255)
         aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                                                                         <  1:4702614/150‑1 (MQ=255)
             tAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcg                                                                                                                                     <  1:991090/150‑1 (MQ=255)
              aaTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcgg                                                                                                                                    >  1:1479030/1‑150 (MQ=255)
                tGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTa                                                                                                                                  <  1:2947144/150‑1 (MQ=255)
                 ggATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                                 <  2:3202691/150‑1 (MQ=255)
                 ggATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                                 >  2:1626226/1‑150 (MQ=255)
                   aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                               >  2:812346/1‑150 (MQ=255)
                   aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                               >  1:2550533/1‑150 (MQ=255)
                   aTTAAGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                               >  2:3234474/1‑150 (MQ=255)
                      aCGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATg                                                                                                                            <  1:3953860/150‑1 (MQ=255)
                        gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGcc                                                                                                                          >  2:485058/1‑150 (MQ=255)
                          cAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTg                                                                                                                        >  1:1594427/1‑150 (MQ=255)
                              ggTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCg                                                                                                                    >  1:2931466/1‑150 (MQ=255)
                               gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                   >  1:4998594/1‑150 (MQ=255)
                                tAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGAc                                                                                                                  <  2:2102901/150‑1 (MQ=255)
                                      gCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaaa                                                                                                            >  2:2525388/1‑150 (MQ=255)
                                      gCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaaa                                                                                                            >  2:1702158/1‑150 (MQ=255)
                                         ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATcc                                                                                                         <  2:4516842/150‑1 (MQ=255)
                                         ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATcc                                                                                                         >  2:3884871/1‑150 (MQ=255)
                                         ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATcc                                                                                                         <  1:2798223/150‑1 (MQ=255)
                                          gCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCg                                                                                                        >  2:2100000/1‑150 (MQ=255)
                                            aGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGc                                                                                                      <  1:37565/150‑1 (MQ=255)
                                               aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATACGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                                                                                   <  1:426322/150‑1 (MQ=255)
                                                  cAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                                <  1:2506906/150‑1 (MQ=255)
                                                     tGCGGATGAGAAACTGGATCGCGCGAGCCTGACTATATTCATTACCAGCTATGTGGTTGGGCAGGCGCTTATGGCCGATAAGATTAAACTCAACAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTgg                                                                                             <  2:1181529/150‑1 (MQ=255)
                                                        ggATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTc                                                                                          <  2:1471723/150‑1 (MQ=255)
                                                         gATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                                                                         <  1:2249026/150‑1 (MQ=255)
                                                          aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                                                                        >  2:5099478/1‑150 (MQ=255)
                                                          aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                                                                        >  2:1836633/1‑150 (MQ=255)
                                                           tGAGAAACTGGATCCCGCGAGTCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATc                                                                                       >  2:1406132/1‑150 (MQ=255)
                                                           tGAGAAACTGGATCCCGCGAGTCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATc                                                                                       >  2:3984304/1‑150 (MQ=255)
                                                             agaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCgg                                                                                     >  2:4234007/1‑150 (MQ=255)
                                                                 aCTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt                                                                                 >  1:3515545/1‑150 (MQ=255)
                                                                    ggATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGtt                                                                              >  1:2210798/1‑150 (MQ=255)
                                                                     gATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTc                                                                             >  1:4188449/1‑150 (MQ=255)
                                                                     gATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTc                                                                             <  1:603245/150‑1 (MQ=255)
                                                                       tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                                                                           <  2:3373999/150‑1 (MQ=255)
                                                                        cccGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                                                                          >  1:4869438/1‑150 (MQ=255)
                                                                        cccGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                                                                          <  1:1913697/150‑1 (MQ=255)
                                                                         ccGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCa                                                                         >  1:1399186/1‑150 (MQ=255)
                                                                           gcgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaaa                                                                       <  1:107991/150‑1 (MQ=255)
                                                                             gAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCTGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAca                                                                     >  1:4551899/1‑149 (MQ=255)
                                                                             gAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                     >  2:3558247/1‑150 (MQ=255)
                                                                             gAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                     <  2:1157247/150‑1 (MQ=255)
                                                                              aGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                                                                    <  2:3031876/150‑1 (MQ=255)
                                                                                ccTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                  <  1:5147330/150‑1 (MQ=255)
                                                                                ccTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                  <  1:1405710/150‑1 (MQ=255)
                                                                                      tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCgggggcgtc                                                            >  2:4755630/1‑144 (MQ=255)
                                                                                      tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCa                                                            <  1:4456479/150‑1 (MQ=255)
                                                                                      tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCa                                                            <  2:684793/150‑1 (MQ=255)
                                                                                      tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCa                                                            <  2:293630/150‑1 (MQ=255)
                                                                                           tCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGttt                                                       >  2:1697110/1‑150 (MQ=255)
                                                                                           tCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGttt                                                       <  1:2742003/150‑1 (MQ=255)
                                                                                            cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                      <  1:4290804/150‑1 (MQ=255)
                                                                                            cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                      <  1:4290653/150‑1 (MQ=255)
                                                                                            cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                      <  1:4119469/150‑1 (MQ=255)
                                                                                            cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                      <  2:2203953/150‑1 (MQ=255)
                                                                                            cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                      <  1:1898800/150‑1 (MQ=255)
                                                                                             attaCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCg                                                     <  2:3693665/147‑1 (MQ=255)
                                                                                                aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTg                                                  <  1:756145/150‑1 (MQ=255)
                                                                                                  cAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGc                                                <  2:1907299/150‑1 (MQ=255)
                                                                                                    gCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAg                                              >  1:3055855/1‑150 (MQ=255)
                                                                                                    gCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAg                                              >  2:717113/1‑150 (MQ=255)
                                                                                                       aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                           >  2:1216775/1‑150 (MQ=255)
                                                                                                         gtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTg                                         >  1:1174995/1‑150 (MQ=255)
                                                                                                            gTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAc                                      <  2:4780038/150‑1 (MQ=255)
                                                                                                             ttGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACa                                     >  2:3998947/1‑150 (MQ=255)
                                                                                                             ttGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACa                                     >  2:1983851/1‑150 (MQ=255)
                                                                                                               gggCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaaa                                   <  2:4133920/150‑1 (MQ=255)
                                                                                                               gggCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaaa                                   >  2:3711164/1‑150 (MQ=255)
                                                                                                                ggCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTCATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAg                                  >  2:4785689/1‑150 (MQ=255)
                                                                                                                 gCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACGAAgg                                 <  2:4932856/150‑1 (MQ=255)
                                                                                                                      cgcTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                            <  1:626524/150‑1 (MQ=255)
                                                                                                                       gcTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCAGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGAt                           >  2:1335100/1‑150 (MQ=255)
                                                                                                                         ttAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGGGTGATTa                         >  2:2550078/1‑150 (MQ=255)
                                                                                                                            aGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATcc                      >  2:4375411/1‑150 (MQ=255)
                                                                                                                             ggCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCa                     <  2:3124947/150‑1 (MQ=255)
                                                                                                                             ggCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCa                     <  1:4913599/150‑1 (MQ=255)
                                                                                                                              gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAg                    <  1:4373079/150‑1 (MQ=255)
                                                                                                                              gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAg                    <  2:2125526/150‑1 (MQ=255)
                                                                                                                                 gATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTcc                 <  2:3542003/150‑1 (MQ=255)
                                                                                                                                    aaGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGt              <  1:85617/150‑1 (MQ=255)
                                                                                                                                     aGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCTCTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTa             <  1:3234474/150‑1 (MQ=255)
                                                                                                                                       aTTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt           <  2:495114/150‑1 (MQ=255)
                                                                                                                                       aTTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt           >  1:232499/1‑150 (MQ=255)
                                                                                                                                       aTTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt           >  1:1995054/1‑150 (MQ=255)
                                                                                                                                           aaCTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACg       <  2:3000104/150‑1 (MQ=255)
                                                                                                                                           aaCTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACg       <  1:1797810/150‑1 (MQ=255)
                                                                                                                                            aCTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGc      >  2:3444344/1‑150 (MQ=255)
                                                                                                                                            aCTCAACAATATGGTGACGGTCGGTAAAGATGCCTGGGAGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAAAAAGGTGTGATTATCCAGTCCGGTAATGACGc      <  2:2630053/150‑1 (MQ=255)
                                                                                                                                              tCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGTCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCt    >  1:4674510/1‑150 (MQ=255)
                                                                                                                                              tCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCt    <  1:4971135/150‑1 (MQ=255)
                                                                                                                                               cACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTg   <  1:2117617/150‑1 (MQ=255)
                                                                                                                                                aCCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGt  <  1:401465/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGT  >  CP009273/876300‑876593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTAT  >  CP009273/876298‑876595
                                                                                                                                                     |                                                                                                                                                    
CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                                                                      <  SRR19973063.3185166/150‑1 (MQ=60)
CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                                                                      <  SRR19973063.1288917/150‑1 (MQ=60)
CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                                                                      <  SRR19973063.3185254/150‑1 (MQ=60)
 GCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAA                                                                                                                                                     >  SRR19973063.1402452/1‑150 (MQ=60)
    TGAATTGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGTCAATGCGGATGAGAAACTGGATCCCGCGAGACTGACTAAAATCATTACCAGCTATGTGGTTGGTCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATAT                                                                                                                                                  <  SRR19973063.1184926/150‑1 (MQ=60)
     GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATG                                                                                                                                                 >  SRR19973063.1090383/1‑150 (MQ=60)
       ATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGT                                                                                                                                               <  SRR19973063.4349956/150‑1 (MQ=60)
        TGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTG                                                                                                                                              >  SRR19973063.1643363/1‑150 (MQ=60)
        TGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTG                                                                                                                                              >  SRR19973063.3126177/1‑150 (MQ=60)
          GATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGAC                                                                                                                                            >  SRR19973063.2655518/1‑150 (MQ=60)
          GATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAC                                                                                                                                            <  SRR19973063.347805/150‑1 (MQ=60)
          GATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGGGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAC                                                                                                                                            >  SRR19973063.4464498/1‑150 (MQ=60)
           ATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACG                                                                                                                                           <  SRR19973063.4717600/150‑1 (MQ=60)
           ATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACG                                                                                                                                           <  SRR19973063.567572/150‑1 (MQ=60)
              TTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTC                                                                                                                                        <  SRR19973063.5061661/150‑1 (MQ=60)
               TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTCTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGTTGACGGTCG                                                                                                                                       >  SRR19973063.5008095/1‑150 (MQ=60)
               TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCG                                                                                                                                       <  SRR19973063.994079/150‑1 (MQ=60)
               TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCG                                                                                                                                       >  SRR19973063.2942658/1‑150 (MQ=60)
                AATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGG                                                                                                                                      >  SRR19973063.1483547/1‑150 (MQ=60)
                AATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGG                                                                                                                                      >  SRR19973063.1483550/1‑150 (MQ=60)
                  TGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTA                                                                                                                                    <  SRR19973063.2956566/150‑1 (MQ=60)
                    GATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAA                                                                                                                                  <  SRR19973063.4687499/150‑1 (MQ=60)
                     ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAG                                                                                                                                 >  SRR19973063.2558662/1‑150 (MQ=60)
                        ACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATG                                                                                                                              <  SRR19973063.3966507/150‑1 (MQ=60)
                        ACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATG                                                                                                                              <  SRR19973063.3745113/150‑1 (MQ=60)
                           CCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCT                                                                                                                           <  SRR19973063.2552705/150‑1 (MQ=60)
                            CAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTG                                                                                                                          >  SRR19973063.1599319/1‑150 (MQ=60)
                            CAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTG                                                                                                                          >  SRR19973063.3703882/1‑150 (MQ=60)
                               CGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGC                                                                                                                       >  SRR19973063.2660796/1‑150 (MQ=60)
                                GGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCG                                                                                                                      >  SRR19973063.2940812/1‑150 (MQ=60)
                                GGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCG                                                                                                                      <  SRR19973063.2106845/150‑1 (MQ=60)
                                 GTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGA                                                                                                                     >  SRR19973063.5014736/1‑150 (MQ=60)
                                       TGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAA                                                                                                               >  SRR19973063.4426739/1‑150 (MQ=60)
                                       TGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAA                                                                                                               <  SRR19973063.91046/150‑1 (MQ=60)
                                           GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                           <  SRR19973063.2807217/150‑1 (MQ=60)
                                           GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                           <  SRR19973063.4965786/150‑1 (MQ=60)
                                           GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCAGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                           <  SRR19973063.4965806/150‑1 (MQ=60)
                                              AGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGC                                                                                                        <  SRR19973063.37713/150‑1 (MQ=60)
                                                 AGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATACGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACT                                                                                                     <  SRR19973063.427513/150‑1 (MQ=60)
                                                    CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                  <  SRR19973063.2514903/150‑1 (MQ=60)
                                                          GGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTC                                                                                            <  SRR19973063.4367147/150‑1 (MQ=60)
                                                           GATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATCAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCA                                                                                           >  SRR19973063.5022740/1‑150 (MQ=60)
                                                           GATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCA                                                                                           <  SRR19973063.2256182/150‑1 (MQ=60)
                                                              GAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCG                                                                                        <  SRR19973063.4502300/150‑1 (MQ=60)
                                                                GAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATCAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGT                                                                                      >  SRR19973063.304679/1‑150 (MQ=60)
                                                                   ACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT                                                                                   >  SRR19973063.3526786/1‑150 (MQ=60)
                                                                      GGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTT                                                                                >  SRR19973063.2217910/1‑150 (MQ=60)
                                                                      GGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTT                                                                                >  SRR19973063.2217765/1‑150 (MQ=60)
                                                                       GATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTC                                                                               <  SRR19973063.605017/150‑1 (MQ=60)
                                                                       GATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTC                                                                               >  SRR19973063.4201823/1‑150 (MQ=60)
                                                                         TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                                                                             <  SRR19973063.2677971/150‑1 (MQ=60)
                                                                         TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACCCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCC                                                                             <  SRR19973063.419576/150‑1 (MQ=60)
                                                                          CCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGTCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                            <  SRR19973063.1919610/150‑1 (MQ=60)
                                                                          CCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                            <  SRR19973063.1919684/150‑1 (MQ=60)
                                                                          CCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                            >  SRR19973063.606842/1‑150 (MQ=60)
                                                                          CCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                            >  SRR19973063.4885132/1‑150 (MQ=60)
                                                                          CCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTC                                                                            <  SRR19973063.1919679/150‑1 (MQ=60)
                                                                           CCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCA                                                                           >  SRR19973063.2834269/1‑150 (MQ=60)
                                                                           CCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCA                                                                           >  SRR19973063.1403418/1‑150 (MQ=60)
                                                                             GCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAA                                                                         <  SRR19973063.108356/150‑1 (MQ=60)
                                                                               GAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCTGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACA                                                                       >  SRR19973063.4566482/1‑150 (MQ=60)
                                                                               GAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACC                                                                       <  SRR19973063.2128401/150‑1 (MQ=60)
                                                                                  CCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGG                                                                    <  SRR19973063.1409964/150‑1 (MQ=60)
                                                                                  CCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGG                                                                    <  SRR19973063.5163897/150‑1 (MQ=60)
                                                                                  CCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGG                                                                    <  SRR19973063.2476501/150‑1 (MQ=60)
                                                                                       CTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATC                                                               >  SRR19973063.3369676/1‑150 (MQ=60)
                                                                                        TAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCA                                                              <  SRR19973063.4470783/150‑1 (MQ=60)
                                                                                           AATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGT                                                           >  SRR19973063.4178416/1‑150 (MQ=60)
                                                                                            ATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGTTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTT                                                          >  SRR19973063.2885570/1‑150 (MQ=60)
                                                                                            ATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTT                                                          <  SRR19973063.4690730/150‑1 (MQ=60)
                                                                                             TCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTT                                                         <  SRR19973063.3978916/150‑1 (MQ=60)
                                                                                             TCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTT                                                         <  SRR19973063.2750856/150‑1 (MQ=60)
                                                                                              CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCACTATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                        <  SRR19973063.4263029/150‑1 (MQ=60)
                                                                                              CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                        <  SRR19973063.4304440/150‑1 (MQ=60)
                                                                                              CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                        <  SRR19973063.4304287/150‑1 (MQ=60)
                                                                                              CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                        <  SRR19973063.4132657/150‑1 (MQ=60)
                                                                                              CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                        <  SRR19973063.1904740/150‑1 (MQ=60)
                                                                                               ATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG                                                       >  SRR19973063.1256008/1‑150 (MQ=60)
                                                                                               ATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG                                                       <  SRR19973063.1470092/150‑1 (MQ=60)
                                                                                               ATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATAGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG                                                       <  SRR19973063.2330512/150‑1 (MQ=60)
                                                                                                 TACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGT                                                     >  SRR19973063.605195/1‑150 (MQ=60)
                                                                                                 TACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGT                                                     >  SRR19973063.395000/1‑150 (MQ=60)
                                                                                                  ACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTG                                                    <  SRR19973063.758348/150‑1 (MQ=60)
                                                                                                      GCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAG                                                >  SRR19973063.3065649/1‑150 (MQ=60)
                                                                                                        TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                              <  SRR19973063.4286919/150‑1 (MQ=60)
                                                                                                        TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                              <  SRR19973063.4286933/150‑1 (MQ=60)
                                                                                                        TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                              >  SRR19973063.2225666/1‑150 (MQ=60)
                                                                                                         ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                             >  SRR19973063.3419273/1‑150 (MQ=60)
                                                                                                         ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                             >  SRR19973063.4304420/1‑150 (MQ=60)
                                                                                                           GTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTG                                           >  SRR19973063.1178542/1‑150 (MQ=60)
                                                                                                             GGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAA                                         >  SRR19973063.4717930/1‑150 (MQ=60)
                                                                                                                    CAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGT                                  >  SRR19973063.3492680/1‑150 (MQ=60)
                                                                                                                       GCGCTTAAGGCCGATAAGATTAAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTG                               <  SRR19973063.2652303/150‑1 (MQ=60)
                                                                                                                        CGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGA                              <  SRR19973063.628367/150‑1 (MQ=60)
                                                                                                                            TAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAT                          <  SRR19973063.713810/150‑1 (MQ=60)
                                                                                                                            TAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAT                          >  SRR19973063.1611223/1‑150 (MQ=60)
                                                                                                                            TAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAT                          <  SRR19973063.3064992/150‑1 (MQ=60)
                                                                                                                             AAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATC                         >  SRR19973063.27868/1‑150 (MQ=60)
                                                                                                                               GGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCA                       >  SRR19973063.4827616/1‑150 (MQ=60)
                                                                                                                               GGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCA                       <  SRR19973063.4929466/150‑1 (MQ=60)
                                                                                                                                GCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAG                      <  SRR19973063.4387077/150‑1 (MQ=60)
                                                                                                                                 CCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGT                     <  SRR19973063.2652926/150‑1 (MQ=60)
                                                                                                                                 CCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGT                     >  SRR19973063.1529514/1‑150 (MQ=60)
                                                                                                                                      TAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGT                >  SRR19973063.4101254/1‑150 (MQ=60)
                                                                                                                                      AAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGT                <  SRR19973063.85910/150‑1 (MQ=60)
                                                                                                                                       AGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCTCTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTA               <  SRR19973063.3244866/150‑1 (MQ=60)
                                                                                                                                       AGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTA               <  SRR19973063.814685/150‑1 (MQ=60)
                                                                                                                                       AGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTA               <  SRR19973063.2357858/150‑1 (MQ=60)
                                                                                                                                       AGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTA               <  SRR19973063.815013/150‑1 (MQ=60)
                                                                                                                                         ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT             >  SRR19973063.2001284/1‑150 (MQ=60)
                                                                                                                                         ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT             >  SRR19973063.2001285/1‑150 (MQ=60)
                                                                                                                                         ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT             >  SRR19973063.233201/1‑150 (MQ=60)
                                                                                                                                         ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT             <  SRR19973063.1407208/150‑1 (MQ=60)
                                                                                                                                          TAAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATG            >  SRR19973063.4581838/1‑150 (MQ=60)
                                                                                                                                            AAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGAC          >  SRR19973063.4890006/1‑150 (MQ=60)
                                                                                                                                             AACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACG         <  SRR19973063.1803416/150‑1 (MQ=60)
                                                                                                                                             AACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACG         <  SRR19973063.1803379/150‑1 (MQ=60)
                                                                                                                                                TCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGTCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCT      >  SRR19973063.4689419/1‑150 (MQ=60)
                                                                                                                                                TCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCT      <  SRR19973063.4987200/150‑1 (MQ=60)
                                                                                                                                                 CACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTG     <  SRR19973063.2124108/150‑1 (MQ=60)
                                                                                                                                                 CACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTG     <  SRR19973063.2124241/150‑1 (MQ=60)
                                                                                                                                                  ACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGT    <  SRR19973063.402597/150‑1 (MQ=60)
                                                                                                                                                  ACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGT    <  SRR19973063.402590/150‑1 (MQ=60)
                                                                                                                                                   CCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTA   >  SRR19973063.142637/1‑150 (MQ=60)
                                                                                                                                                    CAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTAT  <  SRR19973063.2342115/150‑1 (MQ=60)
                                                                                                                                                    CAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTAT  >  SRR19973063.4518353/1‑150 (MQ=60)
                                                                                                                                                    CAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTAT  >  SRR19973063.4355443/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTAT  >  CP009273/876298‑876595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑