Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,447 G→A 100% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,4470GA100.0% 575.4 / NA 180D89N (GAT→AAT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (81/99);  total (81/99)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

TGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCT  >  CP009273/876302‑876591
                                                                                                                                                 |                                                                                                                                                
tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAat                                                                                                                                                <  2:2488873/148‑1 (MQ=255)
tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAat                                                                                                                                                >  1:2033774/1‑148 (MQ=255)
 gCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                                                                               <  1:1025037/148‑1 (MQ=255)
  cATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAatat                                                                                                                                              >  2:1871495/1‑148 (MQ=255)
  cATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAatat                                                                                                                                              >  1:724569/1‑148 (MQ=255)
   aTGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATg                                                                                                                                             <  1:3322151/148‑1 (MQ=255)
     ggATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGt                                                                                                                                           >  1:1466430/1‑148 (MQ=255)
     ggATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGt                                                                                                                                           >  2:375796/1‑148 (MQ=255)
      gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                                                                          <  2:349122/148‑1 (MQ=255)
       aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGa                                                                                                                                         <  2:484762/148‑1 (MQ=255)
         tttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                                                                       >  1:710949/1‑148 (MQ=255)
             aTGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcg                                                                                                                                   >  2:1995026/1‑148 (MQ=255)
                 aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                               <  1:1342092/148‑1 (MQ=255)
                 aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaa                                                                                                                               <  1:1342078/148‑1 (MQ=255)
                   tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                             >  1:221234/1‑148 (MQ=255)
                   tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                             >  2:536149/1‑148 (MQ=255)
                   tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                                                                             <  2:1398496/148‑1 (MQ=255)
                      gCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATg                                                                                                                          <  2:1178485/148‑1 (MQ=255)
                        cAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGcc                                                                                                                        >  2:2645274/1‑148 (MQ=255)
                          gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTg                                                                                                                      <  2:3417396/148‑1 (MQ=255)
                           cGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                                                                     >  1:562633/1‑148 (MQ=255)
                               aaaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                 <  1:2657458/148‑1 (MQ=255)
                                     cTGGCAGAAGGCAATGCGTATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaa                                                                                                           <  2:3068238/148‑1 (MQ=255)
                                       ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGATCCTGACTAAACTCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                                                                         <  2:2405026/148‑1 (MQ=255)
                                       ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                                                                         <  1:3000382/148‑1 (MQ=255)
                                       ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                                                                         <  1:3001031/148‑1 (MQ=255)
                                         cAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATcc                                                                                                       <  1:2219971/148‑1 (MQ=255)
                                            aaGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGc                                                                                                    >  2:565718/1‑148 (MQ=255)
                                                  aTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                              >  2:1962186/1‑148 (MQ=255)
                                                  aTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                              >  2:1559127/1‑148 (MQ=255)
                                                  aTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                                                                              >  2:2215805/1‑148 (MQ=255)
                                                       gATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                                                                         >  1:2223324/1‑148 (MQ=255)
                                                        aTGAGAAACTGGATCCCGCGAGCCTTACTTTTATAATTACCCGATATGTGTTTGGGGAGTCGCTTAAGGCAGATAATATTAAACTCACCACTATGGTGACGGTGGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTTCGTGGTTc                                                                                        <  2:2812457/148‑1 (MQ=255)
                                                        aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTc                                                                                        <  2:1907339/148‑1 (MQ=255)
                                                        aTGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTc                                                                                        >  2:1874753/1‑148 (MQ=255)
                                                         tGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                                                                       <  1:1761912/148‑1 (MQ=255)
                                                         tGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                                                                       >  2:2721858/1‑148 (MQ=255)
                                                          gagaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                                                                      >  2:2903368/1‑148 (MQ=255)
                                                           agaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATc                                                                                     <  2:3249662/148‑1 (MQ=255)
                                                             aaaCTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCgg                                                                                   <  1:959782/148‑1 (MQ=255)
                                                               aCTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAGGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTa                                                                                 <  1:3521833/148‑1 (MQ=255)
                                                               aCTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTa                                                                                 >  2:2905632/1‑148 (MQ=255)
                                                                cTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGACATCCGGCACTGCGTGGTTCATCGGTaa                                                                                >  2:233962/1‑148 (MQ=255)
                                                                cTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                                                                                <  1:476863/148‑1 (MQ=255)
                                                                  ggATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATg                                                                              <  1:2305149/148‑1 (MQ=255)
                                                                  ggATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATg                                                                              >  1:2830394/1‑148 (MQ=255)
                                                                          cgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                                                                      <  2:2347595/148‑1 (MQ=255)
                                                                          cgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                                                                      >  2:1624595/1‑148 (MQ=255)
                                                                            aGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAc                                                                    <  2:1638971/148‑1 (MQ=255)
                                                                              ccTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                                                                  >  2:129380/1‑148 (MQ=255)
                                                                              ccTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCg                                                                  <  2:1460490/148‑1 (MQ=255)
                                                                                tGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                >  2:3015804/1‑148 (MQ=255)
                                                                                   cTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                             <  2:400711/148‑1 (MQ=255)
                                                                                   cTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                             >  1:1842414/1‑148 (MQ=255)
                                                                                   cTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                             <  2:2989872/148‑1 (MQ=255)
                                                                                   cTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                             <  2:856568/148‑1 (MQ=255)
                                                                                    tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGAt                                                            >  2:2917314/1‑148 (MQ=255)
                                                                                     aaaaTCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATc                                                           <  1:1801999/148‑1 (MQ=255)
                                                                                          cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGtt                                                      <  1:721524/148‑1 (MQ=255)
                                                                                          cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGtt                                                      <  2:1719301/148‑1 (MQ=255)
                                                                                              aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCgg                                                  <  2:710949/148‑1 (MQ=255)
                                                                                              aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCgg                                                  <  2:711133/148‑1 (MQ=255)
                                                                                                  gCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGc                                              <  2:1216941/148‑1 (MQ=255)
                                                                                                   cTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCa                                             >  2:1037640/1‑148 (MQ=255)
                                                                                                    tagggggTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCACTCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAg                                            <  1:2536965/143‑1 (MQ=255)
                                                                                                     aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                           <  2:171954/148‑1 (MQ=255)
                                                                                                     aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                           <  1:1282712/148‑1 (MQ=255)
                                                                                                     aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                           <  2:724569/148‑1 (MQ=255)
                                                                                                     aTGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                           >  2:106436/1‑148 (MQ=255)
                                                                                                     aTGTGGATGGGCAGGCGCGTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                           <  2:3276621/148‑1 (MQ=255)
                                                                                                      tgtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAc                                          >  1:2841934/1‑148 (MQ=255)
                                                                                                      tgtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAc                                          >  1:2311552/1‑148 (MQ=255)
                                                                                                       gtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                         <  1:576293/148‑1 (MQ=255)
                                                                                                          gTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGa                                      <  1:191714/148‑1 (MQ=255)
                                                                                                          gTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGa                                      <  1:2069804/148‑1 (MQ=255)
                                                                                                          gTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGa                                      <  1:2069864/148‑1 (MQ=255)
                                                                                                           ttGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGaa                                     >  1:103263/1‑148 (MQ=255)
                                                                                                              ggCAGGCGCTTAAGGCCGCTAAGAATAAACTCACCAATAAGCTGACCGTCGGTAAAGAAGCCTGGGCGACGGGAAATCCGGAACTGCGTGGTTCATCGGTAATGTTCCACAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaa                                  >  2:1591340/1‑148 (MQ=255)
                                                                                                               gCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaaa                                 >  2:3440264/1‑148 (MQ=255)
                                                                                                                  ggCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgt                              <  1:536149/148‑1 (MQ=255)
                                                                                                                  ggCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgt                              >  2:1310732/1‑148 (MQ=255)
                                                                                                                   gcgcTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtg                             >  1:1202940/1‑148 (MQ=255)
                                                                                                                      cTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                          <  2:1503343/148‑1 (MQ=255)
                                                                                                                      cTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                          <  2:2715274/148‑1 (MQ=255)
                                                                                                                      cTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                          <  2:1529502/148‑1 (MQ=255)
                                                                                                                        tAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACTGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGAtt                        <  1:1833805/148‑1 (MQ=255)
                                                                                                                           ggCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATc                     >  2:115006/1‑148 (MQ=255)
                                                                                                                           ggCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATc                     <  1:1141336/148‑1 (MQ=255)
                                                                                                                            gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCTGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATcc                    <  2:943756/148‑1 (MQ=255)
                                                                                                                            gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTCTcc                    >  2:2532289/1‑148 (MQ=255)
                                                                                                                            gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATcc                    <  2:1568561/148‑1 (MQ=255)
                                                                                                                              cGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAg                  <  2:1842414/148‑1 (MQ=255)
                                                                                                                                aTAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTc                <  2:2871475/148‑1 (MQ=255)
                                                                                                                                 tAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTcc               >  1:413139/1‑148 (MQ=255)
                                                                                                                                  aaGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCg              <  2:2258382/148‑1 (MQ=255)
                                                                                                                                  aaGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGCGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCg              >  1:3070692/1‑148 (MQ=255)
                                                                                                                                       tAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt         >  2:2180214/1‑148 (MQ=255)
                                                                                                                                       tAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt         >  1:1573213/1‑148 (MQ=255)
                                                                                                                                         aaCTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCACAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGa       >  1:2306477/1‑148 (MQ=255)
                                                                                                                                              aCCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCt  >  2:2173678/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCT  >  CP009273/876302‑876591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876300‑876596
                                                                                                                                                   |                                                                                                                                                     
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAAT                                                                                                                                                     >  SRR19973066.2047965/1‑150 (MQ=60)
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAAT                                                                                                                                                     >  SRR19973066.2047964/1‑150 (MQ=60)
  TGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATAT                                                                                                                                                   >  SRR19973066.729897/1‑150 (MQ=60)
  TGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATAT                                                                                                                                                   >  SRR19973066.729942/1‑150 (MQ=60)
   GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATG                                                                                                                                                  <  SRR19973066.1032583/150‑1 (MQ=60)
     ATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGT                                                                                                                                                <  SRR19973066.3344220/150‑1 (MQ=60)
     ATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGT                                                                                                                                                >  SRR19973066.1476780/1‑150 (MQ=60)
      TGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTG                                                                                                                                               <  SRR19973066.1107287/150‑1 (MQ=60)
         ATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACG                                                                                                                                            >  SRR19973066.716404/1‑150 (MQ=60)
         ATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACG                                                                                                                                            >  SRR19973066.716218/1‑150 (MQ=60)
         ATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACG                                                                                                                                            <  SRR19973066.617922/150‑1 (MQ=60)
                  GATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAA                                                                                                                                   >  SRR19973066.525875/1‑150 (MQ=60)
                   ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAG                                                                                                                                  <  SRR19973066.1351818/150‑1 (MQ=60)
                   ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAG                                                                                                                                  <  SRR19973066.1351832/150‑1 (MQ=60)
                   ATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAG                                                                                                                                  >  SRR19973066.223033/1‑150 (MQ=60)
                          CAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTG                                                                                                                           <  SRR19973066.3329900/150‑1 (MQ=60)
                           AGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGG                                                                                                                          >  SRR19973066.566641/1‑150 (MQ=60)
                                 AAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACG                                                                                                                    <  SRR19973066.2675279/150‑1 (MQ=60)
                                   AGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGG                                                                                                                  <  SRR19973066.420479/150‑1 (MQ=60)
                                       CTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAT                                                                                                              <  SRR19973066.1921574/150‑1 (MQ=60)
                                         GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                            <  SRR19973066.3020306/150‑1 (MQ=60)
                                         GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                            <  SRR19973066.3020963/150‑1 (MQ=60)
                                         GGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCC                                                                                                            <  SRR19973066.3020320/150‑1 (MQ=60)
                                           CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                                                                          <  SRR19973066.2235328/150‑1 (MQ=60)
                                           CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                                                                          <  SRR19973066.1281518/150‑1 (MQ=60)
                                           CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                                                                          <  SRR19973066.1665181/150‑1 (MQ=60)
                                                  CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                                                                                   >  SRR19973066.914723/1‑150 (MQ=60)
                                                   AATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGT                                                                                                  <  SRR19973066.569732/150‑1 (MQ=60)
                                                       CGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTT                                                                                              >  SRR19973066.2235513/1‑150 (MQ=60)
                                                       CGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTT                                                                                              >  SRR19973066.2238694/1‑150 (MQ=60)
                                                         GATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCA                                                                                            <  SRR19973066.3015465/150‑1 (MQ=60)
                                                           TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                                                                          <  SRR19973066.1774558/150‑1 (MQ=60)
                                                           TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                                                                          <  SRR19973066.1774578/150‑1 (MQ=60)
                                                             AGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGG                                                                                        >  SRR19973066.2235274/1‑150 (MQ=60)
                                                              GAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGT                                                                                       <  SRR19973066.2402497/150‑1 (MQ=60)
                                                               AAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTA                                                                                      <  SRR19973066.966819/150‑1 (MQ=60)
                                                                 ACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAGGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT                                                                                    <  SRR19973066.3545280/150‑1 (MQ=60)
                                                                  CTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATG                                                                                   >  SRR19973066.2849412/1‑150 (MQ=60)
                                                                  CTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATG                                                                                   <  SRR19973066.480459/150‑1 (MQ=60)
                                                                    GGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTT                                                                                 <  SRR19973066.2320935/150‑1 (MQ=60)
                                                                             GAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACC                                                                        <  SRR19973066.378750/150‑1 (MQ=60)
                                                                                   GACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGA                                                                  >  SRR19973066.1855584/1‑150 (MQ=60)
                                                                                       AAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAG                                                              <  SRR19973066.1814800/150‑1 (MQ=60)
                                                                                            CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC                                                         <  SRR19973066.726867/150‑1 (MQ=60)
                                                                                                      tagggGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCACTCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                               <  SRR19973066.2554209/145‑1 (MQ=60)
                                                                                                      TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                               >  SRR19973066.2861032/1‑150 (MQ=60)
                                                                                                      TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                               >  SRR19973066.2327367/1‑150 (MQ=60)
                                                                                                      TATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAC                                               >  SRR19973066.2326873/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              <  SRR19973066.2009059/150‑1 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              >  SRR19973066.2266279/1‑150 (MQ=60)
                                                                                                       ATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACT                                              <  SRR19973066.1291979/150‑1 (MQ=60)
                                                                                                         GTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTG                                            <  SRR19973066.580349/150‑1 (MQ=60)
                                                                                                           GGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAA                                          >  SRR19973066.104354/1‑150 (MQ=60)
                                                                                                            GTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAC                                         <  SRR19973066.2084193/150‑1 (MQ=60)
                                                                                                            GTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAC                                         <  SRR19973066.2084192/150‑1 (MQ=60)
                                                                                                            GTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAC                                         <  SRR19973066.2084132/150‑1 (MQ=60)
                                                                                                            GTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAC                                         <  SRR19973066.193365/150‑1 (MQ=60)
                                                                                                             TTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACA                                        <  SRR19973066.1317552/150‑1 (MQ=60)
                                                                                                             TTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACA                                        <  SRR19973066.1576401/150‑1 (MQ=60)
                                                                                                                   AGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTG                                  >  SRR19973066.1211311/1‑150 (MQ=60)
                                                                                                                    GGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGT                                 <  SRR19973066.540052/150‑1 (MQ=60)
                                                                                                                      CGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGA                               >  SRR19973066.1176234/1‑150 (MQ=60)
                                                                                                                          TAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACTGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAT                           <  SRR19973066.1846900/150‑1 (MQ=60)
                                                                                                                          TAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTAT                           <  SRR19973066.1846904/150‑1 (MQ=60)
                                                                                                                            AGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCC                         >  SRR19973066.3086665/1‑150 (MQ=60)
                                                                                                                             GGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCA                        <  SRR19973066.1149494/150‑1 (MQ=60)
                                                                                                                             GGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCA                        <  SRR19973066.2629454/150‑1 (MQ=60)
                                                                                                                             GGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCA                        <  SRR19973066.2740168/150‑1 (MQ=60)
                                                                                                                                 GATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCC                    >  SRR19973066.416369/1‑150 (MQ=60)
                                                                                                                                  ATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGCGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCG                   >  SRR19973066.3091222/1‑150 (MQ=60)
                                                                                                                                       ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT              >  SRR19973066.1584553/1‑150 (MQ=60)
                                                                                                                                       ATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAT              >  SRR19973066.1385730/1‑150 (MQ=60)
                                                                                                                                         TAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCACAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGA            >  SRR19973066.2322274/1‑150 (MQ=60)
                                                                                                                                                   AATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  <  SRR19973066.2416735/150‑1 (MQ=60)
                                                                                                                                                   AATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  <  SRR19973066.2936773/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876300‑876596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑