Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,288,669 A→T 64.7% intergenic (‑291/‑314) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,288,6690AT64.7% 292.9 / 287.9 252intergenic (‑291/‑314)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base A (46/43);  new base T (82/81);  total (128/124)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 262 total aligned reads displayed.

GCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCCGCT  >  CP009273/1288522‑1288818
                                                                                                                                                   |                                                                                                                                                     
gCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTAt                                                                                                                                                     >  2:701161/1‑150 (MQ=255)
gCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAAt                                                                                                                                                     <  2:791294/150‑1 (MQ=255)
 ccAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTAtt                                                                                                                                                    <  2:2984264/150‑1 (MQ=255)
  cAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTc                                                                                                                                                   <  1:2530770/150‑1 (MQ=255)
    gAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTccc                                                                                                                                                 >  2:4209696/1‑150 (MQ=255)
    gAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTccc                                                                                                                                                 >  2:1675783/1‑150 (MQ=255)
    gAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTccc                                                                                                                                                 >  2:1675825/1‑150 (MQ=255)
     aaaaGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCt                                                                                                                                                >  2:3906665/1‑150 (MQ=255)
       aaGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTAt                                                                                                                                              <  2:2252639/150‑1 (MQ=255)
         gACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTa                                                                                                                                            <  1:2966139/150‑1 (MQ=255)
           cgcgGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAg                                                                                                                                          >  1:1079735/1‑150 (MQ=255)
           cgcgGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAg                                                                                                                                          >  1:1079267/1‑150 (MQ=255)
           cgcgGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAg                                                                                                                                          >  1:1843774/1‑150 (MQ=255)
              ggCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGa                                                                                                                                       >  2:4516712/1‑150 (MQ=255)
              ggCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGa                                                                                                                                       >  2:3686307/1‑150 (MQ=255)
               gCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGaa                                                                                                                                      >  1:880000/1‑150 (MQ=255)
                 atatAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAatg                                                                                                                                    <  2:823771/150‑1 (MQ=255)
                 atatAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAatg                                                                                                                                    >  1:504645/1‑150 (MQ=255)
                 atatAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAatg                                                                                                                                    >  2:678826/1‑150 (MQ=255)
                  tataGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAatga                                                                                                                                   >  1:2343977/1‑150 (MQ=255)
                       cccTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGaaa                                                                                                                              >  1:1797118/1‑150 (MQ=255)
                       cccTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGaaa                                                                                                                              <  2:1250958/150‑1 (MQ=255)
                         cTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAg                                                                                                                            >  2:4069586/1‑150 (MQ=255)
                         cTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAg                                                                                                                            <  2:1461774/150‑1 (MQ=255)
                          tATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGt                                                                                                                           <  2:1720406/150‑1 (MQ=255)
                          tATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGt                                                                                                                           <  1:3812186/150‑1 (MQ=255)
                           aTTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTa                                                                                                                          >  1:1098516/1‑150 (MQ=255)
                           aTTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTa                                                                                                                          >  1:44485/1‑150 (MQ=255)
                           aTTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTa                                                                                                                          <  2:4125389/150‑1 (MQ=255)
                            tttACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAAATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAg                                                                                                                         >  2:2251777/1‑150 (MQ=255)
                              tACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGaa                                                                                                                       >  2:2353997/1‑150 (MQ=255)
                                cacCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACa                                                                                                                     <  2:4272502/150‑1 (MQ=255)
                                cacCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACa                                                                                                                     <  1:61238/150‑1 (MQ=255)
                                  ccGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGt                                                                                                                   <  2:140716/150‑1 (MQ=255)
                                  ccGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGt                                                                                                                   >  2:3569525/1‑150 (MQ=255)
                                   cGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTc                                                                                                                  <  1:4244818/150‑1 (MQ=255)
                                     atgatTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCAGATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGc                                                                                                                >  1:4473348/1‑150 (MQ=255)
                                      tgatTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCa                                                                                                               >  1:4033415/1‑150 (MQ=255)
                                      tgatTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCa                                                                                                               >  2:456859/1‑150 (MQ=255)
                                      tgatTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCa                                                                                                               <  1:1641995/150‑1 (MQ=255)
                                         tttCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATa                                                                                                            <  2:1080442/150‑1 (MQ=255)
                                              cagcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGc                                                                                                       <  1:1495088/150‑1 (MQ=255)
                                               agcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCa                                                                                                      >  1:225830/1‑150 (MQ=255)
                                               agcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCa                                                                                                      >  1:2044499/1‑150 (MQ=255)
                                                gcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCAt                                                                                                     >  2:1378013/1‑150 (MQ=255)
                                                  aCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATgg                                                                                                   >  1:3804327/1‑150 (MQ=255)
                                                       aGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTa                                                                                              <  2:2400101/150‑1 (MQ=255)
                                                        ggTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAg                                                                                             >  2:3003810/1‑150 (MQ=255)
                                                         gTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGGATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATTGACTTAGt                                                                                            >  1:1538016/1‑150 (MQ=255)
                                                            aaaaCTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTAtt                                                                                         <  1:4150153/150‑1 (MQ=255)
                                                                 ttCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACt                                                                                    <  2:3958495/150‑1 (MQ=255)
                                                                  tCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTa                                                                                   >  2:3238628/1‑150 (MQ=255)
                                                                  tCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTa                                                                                   >  1:4324313/1‑150 (MQ=255)
                                                                   ccTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAt                                                                                  >  1:3209416/1‑150 (MQ=255)
                                                                     tGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAtct                                                                                >  1:3472230/1‑150 (MQ=255)
                                                                     tGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAtct                                                                                >  1:1671841/1‑150 (MQ=255)
                                                                     tGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTCTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAtct                                                                                >  2:3357048/1‑150 (MQ=255)
                                                                          cATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTgg                                                                           <  1:3686307/150‑1 (MQ=255)
                                                                            tGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACATTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAg                                                                         <  1:3700267/150‑1 (MQ=255)
                                                                                acaTTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTACTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCa                                                                     >  2:1521226/1‑150 (MQ=255)
                                                                                  aTTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAAc                                                                   >  2:1847436/1‑150 (MQ=255)
                                                                                    tttATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACag                                                                 <  1:1909887/150‑1 (MQ=255)
                                                                                     ttATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACaga                                                                <  1:2651099/150‑1 (MQ=255)
                                                                                      tATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACagag                                                               >  2:2313146/1‑150 (MQ=255)
                                                                                       aTGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAgg                                                              <  2:4324313/150‑1 (MQ=255)
                                                                                              gaTTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTa                                                       >  1:2622960/1‑150 (MQ=255)
                                                                                               aTTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTAc                                                      <  1:1297180/150‑1 (MQ=255)
                                                                                                ttATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACt                                                     >  1:3159171/1‑150 (MQ=255)
                                                                                                 tATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACtt                                                    <  1:2095172/150‑1 (MQ=255)
                                                                                                     gTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTgcgc                                                >  1:3379049/1‑150 (MQ=255)
                                                                                                       aGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCaa                                              <  2:3230445/150‑1 (MQ=255)
                                                                                                        ggCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAAc                                             <  2:303202/150‑1 (MQ=255)
                                                                                                          cTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAg                                           <  2:283461/150‑1 (MQ=255)
                                                                                                          cTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAg                                           <  2:1326430/150‑1 (MQ=255)
                                                                                                            gacgacCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGt                                         >  2:2529459/1‑150 (MQ=255)
                                                                                                            gacgacCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGt                                         >  2:805144/1‑150 (MQ=255)
                                                                                                            gacgacCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGt                                         <  1:4276562/150‑1 (MQ=255)
                                                                                                               gacCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTaa                                      >  2:4440831/1‑150 (MQ=255)
                                                                                                               gacCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTaa                                      >  1:1887526/1‑150 (MQ=255)
                                                                                                                 ccTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAg                                    <  1:3251330/150‑1 (MQ=255)
                                                                                                                   tttCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGAt                                  <  1:919349/150‑1 (MQ=255)
                                                                                                                    ttCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGAtt                                 <  1:662537/150‑1 (MQ=255)
                                                                                                                      cAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATtgt                               <  2:2600676/150‑1 (MQ=255)
                                                                                                                          cttctGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAAt                           <  2:880282/150‑1 (MQ=255)
                                                                                                                          cttctGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAAt                           <  2:1840688/150‑1 (MQ=255)
                                                                                                                           ttctGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATa                          <  1:2052296/150‑1 (MQ=255)
                                                                                                                            tctGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAg                         <  2:1613467/150‑1 (MQ=255)
                                                                                                                             ctGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGt                        <  1:3362166/150‑1 (MQ=255)
                                                                                                                                tATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTAc                     <  2:3874371/150‑1 (MQ=255)
                                                                                                                                tATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTAc                     >  2:3402458/1‑150 (MQ=255)
                                                                                                                                    aGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACcagc                 >  1:3862549/1‑150 (MQ=255)
                                                                                                                                      ttGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACcagcag               >  1:3103908/1‑150 (MQ=255)
                                                                                                                                            ttACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCAttt         >  2:3822313/1‑150 (MQ=255)
                                                                                                                                            ttACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCAttt         <  2:2540865/150‑1 (MQ=255)
                                                                                                                                            ttACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCAttt         <  2:2622970/150‑1 (MQ=255)
                                                                                                                                               cGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTAcc      >  1:3394420/1‑150 (MQ=255)
                                                                                                                                               cGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTAcc      >  1:3393711/1‑150 (MQ=255)
                                                                                                                                                gagtATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTAccc     >  2:2488838/1‑150 (MQ=255)
                                                                                                                                                gagtATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTAccc     <  1:4451344/150‑1 (MQ=255)
                                                                                                                                                gagtATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTAccc     <  1:3191424/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCCGCT  >  CP009273/1288522‑1288818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  >  CP009273/1288520‑1288815
                                                                                                                                                     |                                                                                                                                                  
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGT                                                                                                                                                    >  SRR19973076.773388/1‑150 (MQ=60)
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGT                                                                                                                                                    >  SRR19973076.773387/1‑150 (MQ=60)
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGT                                                                                                                                                    >  SRR19973076.773374/1‑150 (MQ=60)
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGT                                                                                                                                                    >  SRR19973076.773354/1‑150 (MQ=60)
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGT                                                                                                                                                    >  SRR19973076.773353/1‑150 (MQ=60)
    CAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTC                                                                                                                                                <  SRR19973076.2534452/150‑1 (MQ=60)
    CAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTC                                                                                                                                                <  SRR19973076.3702882/150‑1 (MQ=60)
      GAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCC                                                                                                                                              >  SRR19973076.2912803/1‑150 (MQ=60)
       AAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCT                                                                                                                                             <  SRR19973076.4180888/150‑1 (MQ=60)
       AAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCT                                                                                                                                             <  SRR19973076.3837753/150‑1 (MQ=60)
        AAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTA                                                                                                                                            >  SRR19973076.998068/1‑150 (MQ=60)
           GACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTA                                                                                                                                         <  SRR19973076.682518/150‑1 (MQ=60)
           GACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTA                                                                                                                                         <  SRR19973076.2970452/150‑1 (MQ=60)
           GACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTA                                                                                                                                         <  SRR19973076.3226793/150‑1 (MQ=60)
           GACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTA                                                                                                                                         <  SRR19973076.3226445/150‑1 (MQ=60)
            ACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAA                                                                                                                                        >  SRR19973076.1605780/1‑150 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAG                                                                                                                                       <  SRR19973076.684574/150‑1 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAG                                                                                                                                       >  SRR19973076.1080882/1‑150 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAG                                                                                                                                       >  SRR19973076.1846411/1‑150 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAG                                                                                                                                       >  SRR19973076.4100300/1‑150 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAG                                                                                                                                       >  SRR19973076.1080875/1‑150 (MQ=60)
             CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAG                                                                                                                                       >  SRR19973076.1081343/1‑150 (MQ=60)
              GCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGC                                                                                                                                      <  SRR19973076.4348101/150‑1 (MQ=60)
                GGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGA                                                                                                                                    >  SRR19973076.1082051/1‑150 (MQ=60)
                 GCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAA                                                                                                                                   >  SRR19973076.881580/1‑150 (MQ=60)
                 GCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAA                                                                                                                                   >  SRR19973076.881297/1‑150 (MQ=60)
                 GCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAA                                                                                                                                   <  SRR19973076.4511672/150‑1 (MQ=60)
                   ATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATG                                                                                                                                 >  SRR19973076.505456/1‑150 (MQ=60)
                    TATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGA                                                                                                                                >  SRR19973076.2347349/1‑150 (MQ=60)
                         CCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAA                                                                                                                           >  SRR19973076.1799710/1‑150 (MQ=60)
                            TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGT                                                                                                                        <  SRR19973076.3817782/150‑1 (MQ=60)
                             ATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTA                                                                                                                       >  SRR19973076.3381702/1‑150 (MQ=60)
                             ATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTA                                                                                                                       >  SRR19973076.44577/1‑150 (MQ=60)
                             ATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTA                                                                                                                       >  SRR19973076.1100148/1‑150 (MQ=60)
                                TACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAA                                                                                                                    >  SRR19973076.1382332/1‑150 (MQ=60)
                                  CACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACA                                                                                                                  <  SRR19973076.61377/150‑1 (MQ=60)
                                     CGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTC                                                                                                               <  SRR19973076.4251088/150‑1 (MQ=60)
                                     CGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTC                                                                                                               <  SRR19973076.446924/150‑1 (MQ=60)
                                     CGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTC                                                                                                               >  SRR19973076.3874471/1‑150 (MQ=60)
                                       ATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCAGATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGC                                                                                                             >  SRR19973076.4480010/1‑150 (MQ=60)
                                        TGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCA                                                                                                            >  SRR19973076.4039374/1‑150 (MQ=60)
                                        TGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCA                                                                                                            <  SRR19973076.1644403/150‑1 (MQ=60)
                                        TGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCA                                                                                                            <  SRR19973076.1644402/150‑1 (MQ=60)
                                                CAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGC                                                                                                    <  SRR19973076.1497274/150‑1 (MQ=60)
                                                CAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGC                                                                                                    <  SRR19973076.1497261/150‑1 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCA                                                                                                   <  SRR19973076.3391883/150‑1 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCA                                                                                                   >  SRR19973076.226218/1‑150 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCA                                                                                                   >  SRR19973076.226136/1‑150 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCA                                                                                                   >  SRR19973076.2047487/1‑150 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCA                                                                                                   >  SRR19973076.2047514/1‑150 (MQ=60)
                                                 AGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCA                                                                                                   >  SRR19973076.2048112/1‑150 (MQ=60)
                                                    ACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGG                                                                                                >  SRR19973076.3809910/1‑150 (MQ=60)
                                                           GTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGGATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATTGACTTAGT                                                                                         >  SRR19973076.1540271/1‑150 (MQ=60)
                                                              AAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATT                                                                                      <  SRR19973076.4156282/150‑1 (MQ=60)
                                                              AAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATT                                                                                      <  SRR19973076.1900597/150‑1 (MQ=60)
                                                                    TCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAGGAGCATGGACTTAGTATTGCACTA                                                                                <  SRR19973076.4454440/150‑1 (MQ=60)
                                                                    TCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTA                                                                                >  SRR19973076.4330703/1‑150 (MQ=60)
                                                                     CCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAT                                                                               >  SRR19973076.3214027/1‑150 (MQ=60)
                                                                     CCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTAT                                                                               >  SRR19973076.3214074/1‑150 (MQ=60)
                                                                       TGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCT                                                                             >  SRR19973076.297281/1‑150 (MQ=60)
                                                                       TGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCT                                                                             >  SRR19973076.3477254/1‑150 (MQ=60)
                                                                       TGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCT                                                                             >  SRR19973076.1674285/1‑150 (MQ=60)
                                                                            CATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGG                                                                        <  SRR19973076.3691693/150‑1 (MQ=60)
                                                                              TGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACATTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAG                                                                      <  SRR19973076.3705691/150‑1 (MQ=60)
                                                                                TCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGT                                                                    >  SRR19973076.1093977/1‑150 (MQ=60)
                                                                                 CACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTC                                                                   >  SRR19973076.3389893/1‑150 (MQ=60)
                                                                                    ATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAAC                                                                >  SRR19973076.3552108/1‑150 (MQ=60)
                                                                                     TTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAAAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACA                                                               >  SRR19973076.1834307/1‑150 (MQ=60)
                                                                                     TTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAAAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACA                                                               >  SRR19973076.1834290/1‑150 (MQ=60)
                                                                                      TTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAG                                                              <  SRR19973076.1912638/150‑1 (MQ=60)
                                                                                       TTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGA                                                             <  SRR19973076.2654955/150‑1 (MQ=60)
                                                                                        TATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAG                                                            <  SRR19973076.195995/150‑1 (MQ=60)
                                                                                              GAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTAT                                                      <  SRR19973076.3357813/150‑1 (MQ=60)
                                                                                              GAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTAT                                                      <  SRR19973076.3354941/150‑1 (MQ=60)
                                                                                                GATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTA                                                    <  SRR19973076.538805/150‑1 (MQ=60)
                                                                                                GATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTA                                                    >  SRR19973076.2626780/1‑150 (MQ=60)
                                                                                                GATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTA                                                    >  SRR19973076.2626790/1‑150 (MQ=60)
                                                                                                 ATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTAC                                                   <  SRR19973076.1299092/150‑1 (MQ=60)
                                                                                                  TTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACT                                                  >  SRR19973076.3163755/1‑150 (MQ=60)
                                                                                                  TTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACT                                                  >  SRR19973076.3163950/1‑150 (MQ=60)
                                                                                                  TTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACT                                                  >  SRR19973076.3163980/1‑150 (MQ=60)
                                                                                                   TATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTT                                                 <  SRR19973076.3424240/150‑1 (MQ=60)
                                                                                                   TATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTT                                                 <  SRR19973076.2098215/150‑1 (MQ=60)
                                                                                                   TATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTT                                                 <  SRR19973076.4547090/150‑1 (MQ=60)
                                                                                                    ATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTG                                                >  SRR19973076.2418608/1‑150 (MQ=60)
                                                                                                    ATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTG                                                >  SRR19973076.2418208/1‑150 (MQ=60)
                                                                                                       GTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGC                                             >  SRR19973076.3383947/1‑150 (MQ=60)
                                                                                                       GTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGC                                             >  SRR19973076.4257979/1‑150 (MQ=60)
                                                                                                       GTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGC                                             >  SRR19973076.1104459/1‑150 (MQ=60)
                                                                                                         AGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAA                                           >  SRR19973076.4264190/1‑150 (MQ=60)
                                                                                                           GCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACA                                         <  SRR19973076.2202615/150‑1 (MQ=60)
                                                                                                            CTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAG                                        >  SRR19973076.229652/1‑150 (MQ=60)
                                                                                                              GACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGT                                      <  SRR19973076.4282911/150‑1 (MQ=60)
                                                                                                              GACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGT                                      <  SRR19973076.4282883/150‑1 (MQ=60)
                                                                                                                 GACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAA                                   >  SRR19973076.1890168/1‑150 (MQ=60)
                                                                                                                 GACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAA                                   >  SRR19973076.1890242/1‑150 (MQ=60)
                                                                                                                 GACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAA                                   >  SRR19973076.1890246/1‑150 (MQ=60)
                                                                                                                   CCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAG                                 <  SRR19973076.3256058/150‑1 (MQ=60)
                                                                                                                     TTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGAT                               <  SRR19973076.920709/150‑1 (MQ=60)
                                                                                                                     TTTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGAT                               <  SRR19973076.920707/150‑1 (MQ=60)
                                                                                                                      TTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATT                              <  SRR19973076.663531/150‑1 (MQ=60)
                                                                                                                      TTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATT                              <  SRR19973076.3815216/150‑1 (MQ=60)
                                                                                                                      TTCAGTCTTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATT                              <  SRR19973076.3814821/150‑1 (MQ=60)
                                                                                                                       TCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTG                             <  SRR19973076.659442/150‑1 (MQ=60)
                                                                                                                       TCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTG                             <  SRR19973076.659549/150‑1 (MQ=60)
                                                                                                                            CTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAAT                        >  SRR19973076.2574814/1‑150 (MQ=60)
                                                                                                                            CTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAAT                        >  SRR19973076.2574938/1‑150 (MQ=60)
                                                                                                                             TTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATA                       <  SRR19973076.2055299/150‑1 (MQ=60)
                                                                                                                             TTCTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATA                       <  SRR19973076.2055289/150‑1 (MQ=60)
                                                                                                                               CTGTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGT                     <  SRR19973076.3367043/150‑1 (MQ=60)
                                                                                                                                 GTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCTCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTA                   <  SRR19973076.1540101/150‑1 (MQ=60)
                                                                                                                                 GTATTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTA                   <  SRR19973076.3801674/150‑1 (MQ=60)
                                                                                                                                   ATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACC                 >  SRR19973076.3684903/1‑150 (MQ=60)
                                                                                                                                   ATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACC                 >  SRR19973076.1949899/1‑150 (MQ=60)
                                                                                                                                    TTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCA                <  SRR19973076.1759881/150‑1 (MQ=60)
                                                                                                                                    TTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCA                <  SRR19973076.372894/150‑1 (MQ=60)
                                                                                                                                    TTAGTTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCA                <  SRR19973076.153976/150‑1 (MQ=60)
                                                                                                                                      AGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGC              >  SRR19973076.3868242/1‑150 (MQ=60)
                                                                                                                                      AGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGC              >  SRR19973076.1610805/1‑150 (MQ=60)
                                                                                                                                      AGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGC              >  SRR19973076.3868244/1‑150 (MQ=60)
                                                                                                                                       GTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCA             <  SRR19973076.419516/150‑1 (MQ=60)
                                                                                                                                        TTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAG            >  SRR19973076.3108407/1‑150 (MQ=60)
                                                                                                                                        TTGTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAG            >  SRR19973076.1671582/1‑150 (MQ=60)
                                                                                                                                          GTGTTTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTC          <  SRR19973076.3162750/150‑1 (MQ=60)
                                                                                                                                              TTACGAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTT      <  SRR19973076.3407386/150‑1 (MQ=60)
                                                                                                                                                 CGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACC   >  SRR19973076.3398629/1‑150 (MQ=60)
                                                                                                                                                 CGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACC   >  SRR19973076.3398628/1‑150 (MQ=60)
                                                                                                                                                 CGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACC   >  SRR19973076.3399338/1‑150 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATCGTTACCAGCAGTCATTTACCC  <  SRR19973076.4457825/150‑1 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  <  SRR19973076.3196049/150‑1 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  <  SRR19973076.4457971/150‑1 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  <  SRR19973076.2971467/150‑1 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  <  SRR19973076.2799994/150‑1 (MQ=60)
                                                                                                                                                  GAGTATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  <  SRR19973076.4457974/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATGATGAAAAGTAGAACAGTCGCAATAAGAGCATGGACTTAGTATTGCACTATCTCCTGGAGGTCAACAGAGGGCTATTACTTGCGCAACAGGTTAAAGATTGTGAATAGTTACCAGCAGTCATTTACCC  >  CP009273/1288520‑1288815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑