Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,332,963 C→A 17.9% A154A (GCG→GCT)  mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,332,9630CA17.9% 750.6 / 106.4 255A154A (GCG→GCT) mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (104/105);  new base A (21/25);  total (125/130)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

CGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332815‑3333112
                                                                                                                                                    |                                                                                                                                                     
 gTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAgcgc                                                                                                                                                     >  1:4378879/1‑150 (MQ=255)
     gaAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCa                                                                                                                                                 >  2:289844/1‑150 (MQ=255)
       aaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcg                                                                                                                                               <  1:3268154/150‑1 (MQ=255)
        accaccACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgc                                                                                                                                              >  2:387218/1‑150 (MQ=255)
         ccaccaCACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgcc                                                                                                                                             <  1:276352/150‑1 (MQ=255)
                 caAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATc                                                                                                                                     >  1:1892050/1‑150 (MQ=255)
                 caAGTCACGCCCAGCGCGCTGTTCAGCACAGAAATCAGCGTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACACAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATc                                                                                                                                     <  2:2625160/150‑1 (MQ=255)
                   aGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATccc                                                                                                                                   >  2:3639488/1‑150 (MQ=255)
                   aGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATccc                                                                                                                                   >  2:3602218/1‑150 (MQ=255)
                   aGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATccc                                                                                                                                   <  1:3936220/150‑1 (MQ=255)
                    gTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCa                                                                                                                                  <  1:2685273/150‑1 (MQ=255)
                     tCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCAc                                                                                                                                 >  1:3887948/1‑150 (MQ=255)
                           ccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGaaa                                                                                                                           >  1:2012170/1‑150 (MQ=255)
                            cAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAg                                                                                                                          <  2:1460551/150‑1 (MQ=255)
                            cAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAg                                                                                                                          <  2:3826814/150‑1 (MQ=255)
                             aGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGt                                                                                                                         >  2:1118585/1‑150 (MQ=255)
                             aGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGt                                                                                                                         >  2:552084/1‑150 (MQ=255)
                                 cgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAg                                                                                                                     <  2:3822147/150‑1 (MQ=255)
                                  gcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGACAGTTCAGa                                                                                                                    >  2:3889922/1‑150 (MQ=255)
                                      ttCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGc                                                                                                                >  2:3117910/1‑150 (MQ=255)
                                         aGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAtt                                                                                                             <  2:1922813/150‑1 (MQ=255)
                                           cTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAg                                                                                                           <  1:1945339/150‑1 (MQ=255)
                                           cTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAg                                                                                                           <  1:375564/150‑1 (MQ=255)
                                              aGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGttt                                                                                                        <  1:1164321/150‑1 (MQ=255)
                                              aGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGttt                                                                                                        >  2:1569522/1‑150 (MQ=255)
                                               gAAATCAGCGTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTa                                                                                                       <  2:3300855/150‑1 (MQ=255)
                                                 aaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGc                                                                                                     <  2:3134301/150‑1 (MQ=255)
                                                 aaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGc                                                                                                     <  1:4524769/150‑1 (MQ=255)
                                                     aGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCt                                                                                                 <  1:1685745/150‑1 (MQ=255)
                                                      gCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTc                                                                                                >  1:335196/1‑150 (MQ=255)
                                                       cTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTcc                                                                                               >  1:1317/1‑150 (MQ=255)
                                                        ttCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCa                                                                                              <  1:3718670/150‑1 (MQ=255)
                                                        gtCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCa                                                                                              <  2:4249791/149‑1 (MQ=255)
                                                         tCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCAc                                                                                             >  1:2915998/1‑150 (MQ=255)
                                                           aCCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGc                                                                                           >  2:1873499/1‑150 (MQ=255)
                                                            ccAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCa                                                                                          <  2:4343796/150‑1 (MQ=255)
                                                            ccAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCa                                                                                          >  2:2759674/1‑150 (MQ=255)
                                                              aGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGc                                                                                        >  1:1611282/1‑150 (MQ=255)
                                                              aGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCCCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGc                                                                                        >  1:534963/1‑150 (MQ=255)
                                                                  cGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCa                                                                                    <  2:797091/150‑1 (MQ=255)
                                                                  cGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCa                                                                                    <  2:797135/150‑1 (MQ=255)
                                                                   gCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACTTGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCAc                                                                                   >  1:4462795/1‑150 (MQ=255)
                                                                   gCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCAc                                                                                   <  1:2866460/150‑1 (MQ=255)
                                                                    cccATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACg                                                                                  >  2:4539937/1‑150 (MQ=255)
                                                                    cccATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCCCCACGCAGCCCCACg                                                                                  <  1:2113165/150‑1 (MQ=255)
                                                                     ccATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACgg                                                                                 >  1:3469450/1‑150 (MQ=255)
                                                                       aTGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGcc                                                                               <  1:4286642/150‑1 (MQ=255)
                                                                         ggTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTc                                                                             <  2:4021309/150‑1 (MQ=255)
                                                                         ggTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTc                                                                             <  1:786611/150‑1 (MQ=255)
                                                                          gTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTcc                                                                            >  2:291453/1‑150 (MQ=255)
                                                                            gATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAg                                                                          <  2:2052147/150‑1 (MQ=255)
                                                                            gATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAg                                                                          >  2:1040893/1‑150 (MQ=255)
                                                                            gATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAg                                                                          <  1:4274818/150‑1 (MQ=255)
                                                                              tGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCt                                                                        >  2:105483/1‑150 (MQ=255)
                                                                               gggATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCtt                                                                       >  1:2409694/1‑150 (MQ=255)
                                                                               gggATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCtt                                                                       <  1:1424852/150‑1 (MQ=255)
                                                                                ggATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtc                                                                      >  2:201429/1‑150 (MQ=255)
                                                                                 gATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtca                                                                     <  1:2456980/150‑1 (MQ=255)
                                                                                 gATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtca                                                                     <  1:1560921/150‑1 (MQ=255)
                                                                                 gATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtca                                                                     >  1:3409571/1‑150 (MQ=255)
                                                                                     ttGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcat                                                                 >  2:2713414/1‑150 (MQ=255)
                                                                                      tGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcatc                                                                <  2:1892050/150‑1 (MQ=255)
                                                                                       gCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcatca                                                               <  1:838072/150‑1 (MQ=255)
                                                                                              aaaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACt                                                        <  1:964747/150‑1 (MQ=255)
                                                                                               aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                       >  2:3928424/1‑150 (MQ=255)
                                                                                               aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                       <  2:3907085/150‑1 (MQ=255)
                                                                                                aaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTAt                                                      >  1:3679166/1‑150 (MQ=255)
                                                                                                 cggTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATg                                                     <  2:1695237/149‑1 (MQ=255)
                                                                                                    ttCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaa                                                  >  2:11866/1‑150 (MQ=255)
                                                                                                       atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaacaa                                               >  2:1948725/1‑150 (MQ=255)
                                                                                                       atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaacaa                                               <  1:853903/150‑1 (MQ=255)
                                                                                                         caAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATg                                             <  1:3098577/150‑1 (MQ=255)
                                                                                                          aaaCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATgg                                            <  1:3117910/150‑1 (MQ=255)
                                                                                                          aaaCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATgg                                            >  1:3039857/1‑150 (MQ=255)
                                                                                                              atgatgAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGcgcg                                        >  1:1764413/1‑150 (MQ=255)
                                                                                                               tgatgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCgg                                       <  1:3602218/150‑1 (MQ=255)
                                                                                                                 atgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGa                                     >  1:2479828/1‑150 (MQ=255)
                                                                                                                     gaTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                                 <  2:2271428/150‑1 (MQ=255)
                                                                                                                         cGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGt                             <  2:1108138/150‑1 (MQ=255)
                                                                                                                          ggCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTa                            >  1:1485384/1‑150 (MQ=255)
                                                                                                                           gCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  1:552084/150‑1 (MQ=255)
                                                                                                                           gCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  1:4095443/150‑1 (MQ=255)
                                                                                                                           gCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  1:2203352/150‑1 (MQ=255)
                                                                                                                            cTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATg                          >  1:2569344/1‑150 (MQ=255)
                                                                                                                             tCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGt                         <  1:4373890/150‑1 (MQ=255)
                                                                                                                             tCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGt                         <  1:4371821/150‑1 (MQ=255)
                                                                                                                              ccAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGtt                        <  2:2025073/150‑1 (MQ=255)
                                                                                                                                aGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      >  1:1992482/1‑150 (MQ=255)
                                                                                                                                aGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      <  1:1827593/150‑1 (MQ=255)
                                                                                                                                    cAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCCTTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCa                  >  2:659486/1‑150 (MQ=255)
                                                                                                                                    cAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCa                  >  2:2322458/1‑150 (MQ=255)
                                                                                                                                      aTCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGt                >  1:1020991/1‑150 (MQ=255)
                                                                                                                                       tCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTg               <  1:2192622/150‑1 (MQ=255)
                                                                                                                                        cGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTgg              >  2:4095466/1‑150 (MQ=255)
                                                                                                                                         gCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGa             >  1:483802/1‑150 (MQ=255)
                                                                                                                                            cgcgCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAg          <  2:2162102/150‑1 (MQ=255)
                                                                                                                                               gcCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCg       <  2:3286700/150‑1 (MQ=255)
                                                                                                                                                ccAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGa      >  2:3092994/1‑150 (MQ=255)
                                                                                                                                                ccAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGa      <  2:748138/150‑1 (MQ=255)
                                                                                                                                                ccAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGa      <  2:3153569/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332815‑3333112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A